BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,382,288	A→C	7.6%	intergenic (+36/‑1)	glyV → / → glyX	tRNA‑Gly/tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,382,288	0	A	C	7.6%	384.2 / 7.5	165	intergenic (+36/‑1)	glyV/glyX	tRNA‑Gly/tRNA‑Gly
Reads supporting (aligned to +/- strand): ref base A (62/87); new base C (2/14); total (64/101)
Fisher's exact test for biased strand distribution p-value = 2.92e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

AAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGC  >  CP009273/4382141‑4382434

aactGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCaa                                                                                                                                                    <  1:1871445/145‑1 (MQ=255)

aaaTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCaa                                                                                                                                                    <  1:1871329/148‑1 (MQ=255)

aaaTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCaa                                                                                                                                                    >  2:1808673/1‑148 (MQ=255)

aaaTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCaa                                                                                                                                                    <  1:1221942/148‑1 (MQ=255)

  aTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGc                                                                                                                                                  <  1:2612771/148‑1 (MQ=255)

  aTGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGc                                                                                                                                                  >  2:3371101/1‑148 (MQ=255)

    gCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCgg                                                                                                                                                <  2:1109363/148‑1 (MQ=255)

    gCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCgg                                                                                                                                                <  1:2910538/148‑1 (MQ=255)

     cGAAAATTTCGAAAGCAAAATTATGTTGTTCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGTGAGTTCGAGTCTCTTTTCCCGCTCAATAATTTGAAATGTGCTGCAAAGAACAGACCACACAAGCggg                                                                                                                                               <  2:1011046/148‑1 (MQ=17)

      gAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGa                                                                                                                                              <  1:2565429/148‑1 (MQ=255)

       aaaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCGGCAAACCCCAGACCACCCACGCgggca                                                                                                                                             >  1:371060/1‑146 (MQ=255)

       aaaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGTAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGaa                                                                                                                                             >  1:371057/1‑148 (MQ=255)

       aaaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGaa                                                                                                                                             <  2:556587/148‑1 (MQ=255)

       aaaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGaa                                                                                                                                             >  1:1432797/1‑148 (MQ=255)

         aaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATa                                                                                                                                           >  1:3258969/1‑148 (MQ=255)

         aaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGAAAAGCACAGACCACCCAAGCGGGAATa                                                                                                                                           >  1:3087425/1‑148 (MQ=255)

          aTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAg                                                                                                                                          <  1:2650733/148‑1 (MQ=255)

             aCGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTc                                                                                                                                       <  2:3142724/148‑1 (MQ=255)

               gAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAg                                                                                                                                     <  2:1490744/148‑1 (MQ=255)

               gAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAg                                                                                                                                     <  2:2140698/148‑1 (MQ=255)

                aaaGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGt                                                                                                                                    <  2:2993594/148‑1 (MQ=255)

                 aaGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCGAGGGGGGATAGCTCAGtt                                                                                                                                   >  2:2323212/1‑148 (MQ=255)

                 aaGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCGGtg                                                                                                                                   >  2:1131829/1‑147 (MQ=255)

                   gCACAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTG‑AAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGt                                                                                                                                <  1:948516/148‑1 (MQ=11)

                      aaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAAGTGCTTCAAAGCACCGACCACCCAATCGGGAATAGCTGCGTTGGTag                                                                                                                              >  1:2449448/1‑148 (MQ=17)

                      aaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTag                                                                                                                              <  1:1202955/148‑1 (MQ=255)

                      aaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTag                                                                                                                              <  1:1175124/148‑1 (MQ=255)

                      aaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTag                                                                                                                              <  1:1175101/148‑1 (MQ=255)

                      aaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTag                                                                                                                              <  1:2604129/148‑1 (MQ=255)

                       aaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAAGTGAAGAAAAGCAAAGTCCACCCGATGGGGTATTGCTGAGTTGGtttg                                                                                                                             >  1:332469/1‑145 (MQ=14)

                               agtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGAcc                                                                                                                     >  1:3249368/1‑148 (MQ=255)

                                  aCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTg                                                                                                                  >  1:972192/1‑148 (MQ=255)

                                       ggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAg                                                                                                             <  2:2275190/148‑1 (MQ=255)

                                        gAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAgg                                                                                                            >  2:1252100/1‑148 (MQ=255)

                                         aaTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGt                                                                                                           <  1:2379864/148‑1 (MQ=255)

                                           tAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                                                                                         <  1:237798/148‑1 (MQ=255)

                                           tAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTGTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                                                                                         <  2:3240731/148‑1 (MQ=255)

                                            aGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                                                        <  1:3027526/148‑1 (MQ=255)

                                            aGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTGTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                                                        <  2:899905/148‑1 (MQ=255)

                                                cAGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTATAAAAGACATTAAAATCACACACCACCCAAGCGGGAATAGCTGTGTTTTTTTTGAACTACCTTGACAAGGACggggac                                                                                                    >  1:1118242/1‑146 (MQ=2)

                                                 aGTTGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcg                                                                                                   <  1:2204936/148‑1 (MQ=255)

                                                   ttGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgcg                                                                                                 >  2:2715761/1‑148 (MQ=255)

                                                   ttGGTAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgcg                                                                                                 >  2:2660929/1‑148 (MQ=255)

                                                       tAGAGCACGACCTTTCCTATGTTGGGGTCGATATTTCGAGTCTCGTTTACCTCTCCAATATTTGAAAAGTGCTGAAAATCTCAGACCTACCAAGCGTGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGtt                                                                                             <  2:3356735/148‑1 (MQ=11)

                                                       tAGAGCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGtt                                                                                             <  1:629387/148‑1 (MQ=255)

                                                         gagCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCg                                                                                           <  2:2716036/148‑1 (MQ=255)

                                                          agCACGACCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                                          <  1:3371101/148‑1 (MQ=255)

                                                            cACGACCTTTCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGt                                                                                        <  2:1257068/148‑1 (MQ=255)

                                                              cGACCTTGCCCAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                                      <  2:3352064/148‑1 (MQ=255)

                                                                aCCTTGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAAGTGCTGAAAATCCCACACCACCCAAGCGGGTATAGCTCAGTTGTTAGTGCACTCACAACCCAAGGGCGGGGTCGCGCGTGCGAGTCTCg                                                                                    >  2:1336092/1‑148 (MQ=11)

                                                                   ttGCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGttt                                                                                 >  2:2869011/1‑148 (MQ=255)

                                                                    tGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                                <  2:1500212/148‑1 (MQ=39)

                                                                     gCCAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTcc                                                                               <  2:1941236/148‑1 (MQ=255)

                                                                       cAAGGTAGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAAGAGCTGCAAAGCACAGACCACCCAAGCGGGTATAGATCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTAGCGTGTTCGAGTATGCTTTCCCg                                                                             >  2:1729521/1‑148 (MQ=14)

                                                                       cAACGTAGGGGTCGCGAGTTCGAGTCACCTTTCCCGCTCAAAAATTTGAAAAGTTCTTCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCg                                                                             <  1:968227/148‑1 (MQ=14)

                                                                              ggggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaa                                                                      <  1:1198021/148‑1 (MQ=39)

                                                                              ggggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaa                                                                      <  1:2510079/148‑1 (MQ=39)

                                                                              ggggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaa                                                                      <  1:747744/148‑1 (MQ=39)

                                                                               gggTCGCCTGTTGGAGTCTCGTTGCCCCCGCCAAAA‑TTGAAAAGTGCTGAAACGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaatt                                                                    <  2:371060/148‑3 (MQ=11)

                                                                                ggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAATAGTGCTGCAAAGCACATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAt                                                                    <  2:2583895/148‑1 (MQ=255)

                                                                                   cgcgAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTg                                                                 >  1:1690262/1‑148 (MQ=255)

                                                                                   cgcgAGTTCGAGTCTCGTTTCCCGCTCCAAAATTGGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTAGCCAAGGTCGGGGACGCGAGGTCGAGACTCGTTTCCCGCGCCCAAATCTg                                                                 >  1:1917365/1‑148 (MQ=14)

                                                                                      gAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaaa                                                              <  1:2980515/148‑1 (MQ=255)

                                                                                       aGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAg                                                             >  1:2081567/1‑148 (MQ=255)

                                                                                         ttCGAGTGTCGTTTCCCGCTCCAAAATTGGAAAAGTGCTGCAAAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTg                                                           <  2:1841075/148‑1 (MQ=255)

                                                                                              gTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTa                                                      <  1:2850274/148‑1 (MQ=255)

                                                                                                 tcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAgg                                                   <  1:1332207/148‑1 (MQ=255)

                                                                                                        ccGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATTAAAGAGCTAAAATGCAAAGaa                                            >  1:1763122/1‑147 (MQ=14)

                                                                                                          gCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGacca                                          <  1:1958523/148‑1 (MQ=255)

                                                                                                           cTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGt                                                                                              >  2:42314/1‑95 (MQ=255)

                                                                                                           cTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGt                                                                                              <  1:42314/95‑1 (MQ=255)

                                                                                                                aaaTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAACGCCCAGACCACCCCAg                                    >  1:1011915/1‑148 (MQ=255)

                                                                                                                   tttGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCgg                                 >  2:488807/1‑148 (MQ=255)

                                                                                                                    ttGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCggg                                >  1:1878154/1‑148 (MQ=255)

                                                                                                                       aaaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTc                                                                          >  1:1002226/1‑103 (MQ=38)

                                                                                                                       aaaaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCGGTCAGGCACAGACCACCCAAGCGGGAAt                             >  1:1317003/1‑148 (MQ=255)

                                                                                                                         aaGTGCTGAAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCATGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAg                           <  2:1432797/148‑1 (MQ=255)

                                                                                                                          tgTGCTGCAAATCACTGACCACGCAAGCGGGATTAGCTCAGTTGGTAGAGCACGACCTTGCAATGGTCGGGGTCGCGAGTGCGAGTCTGGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGc                          <  1:1386857/147‑1 (MQ=17)

                                                                                                                          aGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaattctt                                                                 <  2:2817180/109‑6 (MQ=11)

                                                                                                                           gTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTGAACGTGCTGTAAGGCACAGACCCCCCAAGCGGGAATAGCt                         >  2:1797535/1‑148 (MQ=255)

                                                                                                                              ctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGAACAGACCACCCAAGCGGGAATAGCTCAg                      >  1:2171012/1‑148 (MQ=255)

                                                                                                                               tgcAAAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGt                     <  2:3339279/148‑1 (MQ=255)

                                                                                                                               tgaaaaTCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGt                     <  2:2196304/145‑1 (MQ=255)

                                                                                                                                gcacagcacaGACCACCCACGCGGGAATAGCTCAGGTGGTAGAGCACGACCGTGCCCCGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGtt                    <  2:1917365/148‑1 (MQ=17)

                                                                                                                                gcAAAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGtt                    <  2:3259473/148‑1 (MQ=255)

                                                                                                                                gcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGtt                    <  2:1690262/148‑1 (MQ=255)

                                                                                                                                 cAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTg                   <  1:1252100/148‑1 (MQ=255)

                                                                                                                                  aaaGCACAGACCACGCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTgg                  <  2:3248693/148‑1 (MQ=255)

                                                                                                                                  aaaGCACAGACCACCCCCGCGGGAATAGCTCAGTTGGAAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCACCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTgg                  <  2:3249368/148‑1 (MQ=255)

                                                                                                                                    aGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTa                >  2:1574834/1‑148 (MQ=255)

                                                                                                                                     gCACTGACCACCCACGCGGGAATAGCTCAGTTGGTAGCGCACGACCTTGCCAAGGTCGGGGTCGCGAGTACGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTag               <  2:2335846/148‑1 (MQ=255)

                                                                                                                                      cacaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGACTAGCTGAGTTGGTata              >  2:1710268/1‑146 (MQ=255)

                                                                                                                                        ctgACCACGCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCAAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGc            <  2:2842713/146‑1 (MQ=255)

                                                                                                                                           accaccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACg         >  1:355089/1‑148 (MQ=255)

                                                                                                                                           accaccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACg         <  2:510605/148‑1 (MQ=255)

                                                                                                                                           accaccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACg         >  1:2754418/1‑148 (MQ=255)

                                                                                                                                           accaccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACg         >  1:1840560/1‑148 (MQ=255)

                                                                                                                                            ccaccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGa                               <  1:810994/125‑1 (MQ=255)

                                                                                                                                            ccaccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGa                               >  2:810994/1‑125 (MQ=255)

                                                                                                                                                  aaGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGAACGACCTTGc  >  2:1302637/1‑148 (MQ=255)

AAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGC  >  CP009273/4382141‑4382434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`