Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,388,439 T→A 100% V404E (GTG→GAG)  mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,388,4390TA97.9% 511.1 / 0.1 171V404E (GTG→GAG) mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base A (78/88);  total (78/93)
Fisher's exact test for biased strand distribution p-value = 6.36e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 172 total aligned reads displayed.

GTGGCGGCGGGGCGCAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAGCG  >  CP009273/4388294‑4388580
                                                                                                                                                 |                                                                                                                                             
 tGGCGGCGGGGCGCAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGTCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTa                                                                                                                                            <  2:3187847/148‑1 (MQ=255)
 tGGCGGCGGGGCGCAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGTCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTa                                                                                                                                            <  2:551731/148‑1 (MQ=255)
  ggcggcGGGGCGCAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTAt                                                                                                                                           >  1:2198927/1‑148 (MQ=255)
  ggcggcGGGGCGCAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTAt                                                                                                                                           >  1:2198065/1‑148 (MQ=255)
  ggcggcGGGGCGCAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTAt                                                                                                                                           >  1:2883729/1‑148 (MQ=255)
     ggcggGGCGCAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGc                                                                                                                                        <  1:2211948/148‑1 (MQ=255)
       cggGGCGCAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCa                                                                                                                                      <  1:590714/148‑1 (MQ=255)
         gggCGCAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGc                                                                                                                                    <  2:1515206/148‑1 (MQ=255)
          ggCGCAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCt                                                                                                                                   <  1:3175828/148‑1 (MQ=255)
          ggCGCAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCt                                                                                                                                   >  2:1410178/1‑148 (MQ=255)
            cgcAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCttt                                                                                                                                 <  2:1605626/148‑1 (MQ=255)
            cgcAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCttt                                                                                                                                 <  1:2249759/148‑1 (MQ=255)
            cgcAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCttt                                                                                                                                 <  1:2249061/148‑1 (MQ=255)
                aTCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCa                                                                                                                             >  2:1608024/1‑148 (MQ=255)
                  cACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCaaa                                                                                                                           <  1:12478/148‑1 (MQ=255)
                     tttGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGc                                                                                                                        >  2:1411193/1‑148 (MQ=255)
                      ttGCAGAAGCGGCAGCTCGTGAGCCGGTATCTCCGCGATACACTCCTGCGCAAGCATAATGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAAACGAAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGcc                                                                                                                       <  1:786887/148‑1 (MQ=255)
                           gAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCgcgc                                                                                                                  >  2:369012/1‑148 (MQ=255)
                              ccGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGACGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGa                                                                                                               >  2:407309/1‑148 (MQ=255)
                              ccGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGa                                                                                                               <  2:3489137/148‑1 (MQ=255)
                              ccGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGa                                                                                                               >  2:447869/1‑148 (MQ=255)
                              ccGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGa                                                                                                               >  2:407344/1‑148 (MQ=255)
                              ccGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGa                                                                                                               <  1:2887306/148‑1 (MQ=255)
                              ccGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCGGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGa                                                                                                               <  2:1230361/148‑1 (MQ=255)
                                ggCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACCGCAAGGTGAAGATTATCGCCAGCTTTTGAAAACGCCCGCGCCGATg                                                                                                             <  1:3363440/148‑1 (MQ=255)
                                 gCAGCTCGTGAGCCGGTTGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGATACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGc                                                                                                            <  1:246776/148‑1 (MQ=255)
                                    gCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCaaa                                                                                                         >  1:2843994/1‑148 (MQ=255)
                                    gCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCaaa                                                                                                         >  2:3073317/1‑148 (MQ=255)
                                     cTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCCGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCACAAACACCACGGTTAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCaaaa                                                                                                        <  1:894510/148‑1 (MQ=255)
                                     cTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCaaaa                                                                                                        >  2:1556054/1‑148 (MQ=255)
                                     cTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCaaaa                                                                                                        >  2:1579674/1‑148 (MQ=255)
                                     cTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCaaaa                                                                                                        >  2:3319558/1‑148 (MQ=255)
                                       cGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAAt                                                                                                      >  2:716265/1‑148 (MQ=255)
                                           aGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCCGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTaaa                                                                                                  <  1:2559089/148‑1 (MQ=255)
                                           aGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTaaa                                                                                                  >  1:486914/1‑148 (MQ=255)
                                             ccGGTATCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAg                                                                                                <  2:2564955/148‑1 (MQ=255)
                                             ccGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCGGGCCGAATGCGCAGCCAGGCTACCAGAAACCCCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAg                                                                                                <  1:1311932/148‑1 (MQ=255)
                                               ggTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAgcg                                                                                              <  2:461797/148‑1 (MQ=255)
                                               ggTAGCTCCGCGATACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGATTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAgcg                                                                                              <  1:511687/148‑1 (MQ=255)
                                                   gCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCACACGCCCGCGCCGATGCAAAAATTAAAAGCGCCgg                                                                                          >  2:2781960/1‑148 (MQ=255)
                                                   gCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCgg                                                                                          >  2:2781478/1‑148 (MQ=255)
                                                    cTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAAGGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGa                                                                                         <  2:2297642/148‑1 (MQ=255)
                                                     tCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGac                                                                                        >  1:309042/1‑147 (MQ=255)
                                                     tCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGaa                                                                                        <  2:699294/148‑1 (MQ=255)
                                                         cgcTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGc                                                                                    >  2:100540/1‑148 (MQ=255)
                                                             acacTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGa                                                                                <  1:3164178/148‑1 (MQ=255)
                                                             acacTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGa                                                                                <  2:3479847/148‑1 (MQ=255)
                                                               accccTGCGCCAGCATCCGCCAGTCGCCGGGCGGCCCCCTGGCCGATTGCGCACCCAGGCTACCAAAAACCCCACGGTGACGATTACCGCCCGCTTTTGAAAACGCCCGCGCCGATGAAAAAATAAAAAGCGCCGGAACCGCAGGAAc                                                                              <  1:3383719/145‑1 (MQ=255)
                                                                cTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGATTGCGCAGCCAGGCTACCAGAAACCGCACGGTGAAGAGTATCGCCAGCTTTGGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAAcc                                                                             <  2:1191727/148‑1 (MQ=255)
                                                                cTCCTGCCCCAGAATCAGGCAGTCGTCCGGCTGCCCCCGGGCCGAAGGCGCAGCCAGCCTACCACAAACCCCAAGTTTAAGAGTATGGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAAcc                                                                             <  2:1192013/148‑1 (MQ=255)
                                                                    tGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCa                                                                         >  2:1473601/1‑148 (MQ=255)
                                                                      cgcCAGAATCAGGCAGTCGTCCGGCGGCCCCCTGGCCGAAGGCGCACCCTGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGAAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACt                                                                       <  1:793269/148‑1 (MQ=255)
                                                                        ccAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTATTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTg                                                                     <  2:3012654/148‑1 (MQ=255)
                                                                         cAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTgc                                                                    >  1:144695/1‑148 (MQ=255)
                                                                                     gtcgtcCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCa                                                        <  2:870482/148‑1 (MQ=255)
                                                                                        gtcCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCATAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCagag                                                     <  1:1414779/148‑1 (MQ=255)
                                                                                          cgggCTGCCCCCGGGCGGATTGCTCTCCCTGGATACCATAAACCGCACGGTTATTTTTATGCCCTTTTTTTCAAAACGCCCGCGCCTATGAAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGtt                                                   <  1:1608022/146‑1 (MQ=255)
                                                                                          ccGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGtt                                                   >  1:2878305/1‑148 (MQ=255)
                                                                                          ccGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGtt                                                   <  2:790836/148‑1 (MQ=255)
                                                                                          ccGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGtt                                                   >  2:2143695/1‑148 (MQ=255)
                                                                                          ccGGCTGCCCCCGGGCCTAAGGCGCAGCCCGGCTACCACAACCACCAGGGTTAAGATTATCGCCAGTTTTTGCAAACGCCCGCGCCGATGAAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGtt                                                   <  1:1608024/148‑1 (MQ=255)
                                                                                           cGGCTGCCCCCGGGCCTAATGCGCACCCAGGCTACCACAAACAGCACGGTGAAGAGTATCCCCAGATTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGttt                                                  <  1:961908/148‑1 (MQ=255)
                                                                                             gCTGCCCCCTGGCCGAATGCGCATCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTg                                                <  2:1306237/148‑1 (MQ=255)
                                                                                             gCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTg                                                >  1:2468737/1‑148 (MQ=255)
                                                                                              cTGCCCCCTGGCCGAATGCGCAGCCAGGCTCCCACAAACACCACGGTGAAGATTATCGCCAGCTTTTGAAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTgg                                               <  1:1246277/148‑1 (MQ=255)
                                                                                                gCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGACACCCCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTc                                             <  2:2719497/148‑1 (MQ=255)
                                                                                                gCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTc                                             <  2:965309/148‑1 (MQ=255)
                                                                                                 cccccTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCg                                            >  1:790647/1‑148 (MQ=255)
                                                                                                   cccTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCggg                                          >  1:1040450/1‑148 (MQ=255)
                                                                                                   cccTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCggg                                          >  1:1040630/1‑148 (MQ=255)
                                                                                                    ccTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGt                                         <  1:3112535/148‑1 (MQ=255)
                                                                                                     cTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTTAAGAGTATCGCCAGCTTTTGAAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTa                                        <  1:1283670/148‑1 (MQ=255)
                                                                                                        gCCGACTGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTg                                     <  2:1911770/148‑1 (MQ=255)
                                                                                                        gCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTg                                     <  2:135675/148‑1 (MQ=255)
                                                                                                        gCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTg                                     <  1:559582/148‑1 (MQ=255)
                                                                                                            aaTGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTa                                 <  2:1111626/148‑1 (MQ=255)
                                                                                                            aaTGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTa                                 >  1:644438/1‑148 (MQ=255)
                                                                                                            aaTGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTa                                 >  1:2253120/1‑148 (MQ=255)
                                                                                                              tGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATc                               >  2:2985444/1‑148 (MQ=255)
                                                                                                               gcgcAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCg                              >  2:1123286/1‑148 (MQ=255)
                                                                                                               gcgcAGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCg                              >  2:2837209/1‑148 (MQ=255)
                                                                                                                   aGCCAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCa                          <  2:1806722/148‑1 (MQ=255)
                                                                                                                     ccAGGCTACCAGAAACCGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCAtt                        <  2:1730071/148‑1 (MQ=255)
                                                                                                                     ccAGGCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCAtt                        <  2:2253095/148‑1 (MQ=255)
                                                                                                                        ggCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCg                     >  2:742777/1‑148 (MQ=255)
                                                                                                                        ggCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCg                     >  2:3336327/1‑148 (MQ=255)
                                                                                                                        ggCTACCACAAACCCCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCg                     <  1:113561/148‑1 (MQ=255)
                                                                                                                         gCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGa                    <  2:3387663/148‑1 (MQ=255)
                                                                                                                         gCTACCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGa                    >  2:1621000/1‑148 (MQ=255)
                                                                                                                            aCCAGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACtg                 >  2:2606302/1‑148 (MQ=255)
                                                                                                                              cAGAACCCGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACtgtg               <  1:193302/148‑1 (MQ=255)
                                                                                                                               aGAAACAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGc              >  2:365089/1‑148 (MQ=255)
                                                                                                                                 aaaCAGCAAGGTGAAGAGTATCGCCACCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGt            <  1:2723922/148‑1 (MQ=255)
                                                                                                                                   aCAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTg          <  2:2026604/148‑1 (MQ=255)
                                                                                                                                   aCAGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTg          <  2:2026661/148‑1 (MQ=255)
                                                                                                                                    cAGCACGGTGACGAGTCTCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGc         <  1:2432956/148‑1 (MQ=255)
                                                                                                                                     aGCAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCt        <  1:1994672/148‑1 (MQ=255)
                                                                                                                                       cAAGGTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTgg      >  1:2949378/1‑148 (MQ=255)
                                                                                                                                          ggTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAgc   >  1:270063/1‑148 (MQ=255)
                                                                                                                                          ggTGAAGAGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAgc   >  1:1279821/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
GTGGCGGCGGGGCGCAATCACTTTGCAGAACCGGCAGCTCGTGAGCCGGTAGCTCCGCGCTACACTCCTGCGCCAGCATCAGGCAGTCGTCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAGCG  >  CP009273/4388294‑4388580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑