Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,410,964 A→C 100% I390L (ATC→CTC)  ulaA → L‑ascorbate‑specific enzyme IIC permease component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,410,9640AC99.6% 831.0 / ‑5.4 206I390L (ATC→CTC) ulaAL‑ascorbate‑specific enzyme IIC permease component of PTS
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (108/97);  total (109/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 211 total aligned reads displayed.

TGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAA  >  CP009273/4410819‑4411108
                                                                                                                                                 |                                                                                                                                                
tGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTc                                                                                                                                                <  1:637067/148‑1 (MQ=255)
   cATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGaa                                                                                                                                             <  1:875827/148‑1 (MQ=255)
   cATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGaa                                                                                                                                             <  2:744379/148‑1 (MQ=255)
    aTCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGaaa                                                                                                                                            >  1:2579077/1‑148 (MQ=255)
    aTCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGaaa                                                                                                                                            >  2:2205512/1‑148 (MQ=255)
    aTCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCGGGTGATGGGGATGCTCGaaa                                                                                                                                            >  2:436862/1‑148 (MQ=255)
              ccTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTg                                                                                                                                  <  1:1749400/148‑1 (MQ=255)
                 gCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGt                                                                                                                               <  2:1394671/148‑1 (MQ=255)
                   ggCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCt                                                                                                                             <  2:2454975/148‑1 (MQ=255)
                    gCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTg                                                                                                                            <  1:2607278/148‑1 (MQ=255)
                     ctcctcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTgg                                                                                                                           >  2:205561/1‑148 (MQ=255)
                        ctcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCg                                                                                                                        >  1:1839401/1‑148 (MQ=255)
                        ctcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCg                                                                                                                        >  1:2634336/1‑148 (MQ=255)
                        ctcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCg                                                                                                                        >  2:386713/1‑148 (MQ=255)
                         tcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCgg                                                                                                                       <  1:1168417/148‑1 (MQ=255)
                         tcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACGTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCgg                                                                                                                       >  2:601023/1‑148 (MQ=255)
                             tCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGaa                                                                                                                   >  1:1658691/1‑148 (MQ=255)
                              ccTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGaaa                                                                                                                  <  2:2106054/148‑1 (MQ=255)
                              ccTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGaaa                                                                                                                  <  1:3198662/148‑1 (MQ=255)
                              ccTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGaaa                                                                                                                  <  1:3198483/148‑1 (MQ=255)
                               cTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCCCTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAAc                                                                                                                 <  1:296455/148‑1 (MQ=255)
                               cTGATTAGTCCGGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAAc                                                                                                                 <  2:2705464/148‑1 (MQ=255)
                                  attattCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCa                                                                                                              <  1:2544494/148‑1 (MQ=255)
                                   ttattCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCAc                                                                                                             >  2:2308645/1‑148 (MQ=255)
                                    tattCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCAcc                                                                                                            >  2:3162431/1‑148 (MQ=255)
                                      ttCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACATCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTTGTGATGGGGATGCGCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCgg                                                                                                          >  1:512852/1‑148 (MQ=255)
                                       tCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGCTGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGt                                                                                                         >  1:1405048/1‑148 (MQ=255)
                                       tCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGt                                                                                                         >  1:32992/1‑148 (MQ=255)
                                       tCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCGTTGGTTGCGTCTGGGCGGTGAAACTCACCGGt                                                                                                         >  1:1520835/1‑148 (MQ=255)
                                          tGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCGGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATg                                                                                                      >  2:2964511/1‑148 (MQ=255)
                                           ggCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGa                                                                                                     >  1:1227538/1‑148 (MQ=255)
                                            gCTTTATCCCGATGGTCGTCTCTACCGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGAGGGGGATGCTCGAAATCTTTGGGTGCGTCTGGGCGGTGAAACTCACCGGTATGAg                                                                                                    <  2:2686064/148‑1 (MQ=255)
                                             cTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGt                                                                                                   >  1:1039436/1‑148 (MQ=255)
                                             cTTAATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACAACTTCGGCGGATGGCGTGCGGCGCTGAATATTTGTCGGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAAATCACCGGTATGAGt                                                                                                   >  1:3042849/1‑148 (MQ=255)
                                               ttATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATTGGGATGCTCGAAATATTTGGTTGCGTATGGGCGGTGTAACTCACCGGTATGAGTGc                                                                                                 >  1:1270371/1‑148 (MQ=255)
                                               ttATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGc                                                                                                 <  2:2123543/148‑1 (MQ=255)
                                               ttATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGc                                                                                                 <  2:349185/148‑1 (MQ=255)
                                                   cccGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTgg                                                                                             >  2:718314/1‑148 (MQ=255)
                                                    ccGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGa                                                                                            >  2:3067168/1‑148 (MQ=255)
                                                      gATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATg                                                                                          >  1:3151431/1‑148 (MQ=255)
                                                      gATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATg                                                                                          >  1:1879356/1‑148 (MQ=255)
                                                      gATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATg                                                                                          <  2:3303445/148‑1 (MQ=255)
                                                        tGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATggg                                                                                        >  1:3477339/1‑148 (MQ=255)
                                                           tcttctCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCAt                                                                                     >  1:2015143/1‑148 (MQ=255)
                                                           tcttctCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCAt                                                                                     >  2:322885/1‑148 (MQ=255)
                                                              tctcTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATggt                                                                                  >  2:172000/1‑147 (MQ=255)
                                                              tctcTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGc                                                                                  >  2:1532074/1‑148 (MQ=255)
                                                               ctctAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCg                                                                                 >  2:979945/1‑148 (MQ=255)
                                                               ctctAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCg                                                                                 <  1:1794100/148‑1 (MQ=255)
                                                                 ctAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGa                                                                               <  2:1506888/148‑1 (MQ=255)
                                                                     cGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTgg                                                                           <  2:2478757/148‑1 (MQ=255)
                                                                       ccaccaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGTTTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTc                                                                         >  2:1149764/1‑148 (MQ=255)
                                                                       ccaccaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTc                                                                         >  1:864520/1‑148 (MQ=255)
                                                                       ccaccaTCGGCGTGTTCGCTAACCACTTATGCGGCAGGCGTGGGGCGCTGAAGTTGTGGGTGGAGATGGGGATGCTCGAAAACTTTGGTTGCGTCTGGGCGGTGAAACTAACAGGTATGAGTGAGTGGAAGGGCATAGCGGACTgggc                                                                         >  2:1899094/1‑146 (MQ=255)
                                                                         accaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGa                                                                       <  2:2406070/148‑1 (MQ=255)
                                                                         accaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGa                                                                       >  2:1295875/1‑148 (MQ=255)
                                                                            aTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTc                                                                    >  1:2450339/1‑148 (MQ=255)
                                                                             tCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCt                                                                   >  2:594780/1‑148 (MQ=255)
                                                                                gCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGc                                                                >  1:2253708/1‑148 (MQ=255)
                                                                                 cGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTTTATGGTGATGGGGATGCTCGAAATCTTTGGTTTCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATTGGCATGGCGGAATGGTCGATACTGGCa                                                               >  1:2927042/1‑148 (MQ=255)
                                                                                       cGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCAccgccg                                                         <  2:1580266/148‑1 (MQ=255)
                                                                                       cGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCAccgccg                                                         <  2:1839401/148‑1 (MQ=255)
                                                                                       cGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCAccgccg                                                         <  2:2634336/148‑1 (MQ=255)
                                                                                        gCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCga                                                        <  1:1868840/148‑1 (MQ=255)
                                                                                        gCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCga                                                        <  1:3366345/148‑1 (MQ=255)
                                                                                         cTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCgat                                                       <  2:2551594/148‑1 (MQ=255)
                                                                                         cTAACCACGTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCgat                                                       <  1:2679379/148‑1 (MQ=255)
                                                                                             ccACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGatgatg                                                   >  1:78861/1‑148 (MQ=255)
                                                                                             ccACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGatgatg                                                   >  2:2387918/1‑148 (MQ=255)
                                                                                             ccACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGatgatg                                                   >  2:1739088/1‑148 (MQ=255)
                                                                                               tcTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCTCCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCa                                                 <  2:2175266/147‑1 (MQ=255)
                                                                                               aCTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCa                                                 >  1:3008577/1‑148 (MQ=255)
                                                                                                  tCGGCGGCTGGCGTGCGGCGCTGAAGCTTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAgg                                              <  2:2003628/148‑1 (MQ=255)
                                                                                                          tGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTtcttct                                      <  2:2429784/148‑1 (MQ=255)
                                                                                                           ggCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTtctc                                     >  2:1101201/1‑148 (MQ=255)
                                                                                                            gCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTcc                                    >  1:3490581/1‑148 (MQ=255)
                                                                                                             cgagcggcgCTGAAGATGTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCAGGATGGGCATGGCGGACGGGTCGATTCTGCCACCGCCGATGATGCAAGGCTTCTTCTCCa                                   <  2:1976412/145‑1 (MQ=255)
                                                                                                                  ggcgCTGAAGATTTGTCTTGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGt                              <  2:16035/148‑1 (MQ=255)
                                                                                                                  ggcgCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGt                              <  2:3038518/148‑1 (MQ=255)
                                                                                                                      cTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCg                          >  1:1245299/1‑148 (MQ=255)
                                                                                                                        gAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGcc                        <  1:2878135/148‑1 (MQ=255)
                                                                                                                          aGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCtt                      <  2:2842811/148‑1 (MQ=255)
                                                                                                                           gATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCttt                     <  1:158716/148‑1 (MQ=255)
                                                                                                                           gATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCttt                     <  1:3189638/148‑1 (MQ=255)
                                                                                                                              ttGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATg                  >  1:1483375/1‑148 (MQ=255)
                                                                                                                               tGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATgg                 <  2:1842906/148‑1 (MQ=255)
                                                                                                                               tGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATgg                 <  2:1569262/148‑1 (MQ=255)
                                                                                                                                gTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGc                <  1:463516/148‑1 (MQ=255)
                                                                                                                                  cTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCg              >  1:1634264/1‑148 (MQ=255)
                                                                                                                                    ggTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtc            >  1:1170220/1‑148 (MQ=255)
                                                                                                                                     gTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtca           >  2:2860638/1‑148 (MQ=255)
                                                                                                                                      tGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtcat          <  2:3341505/148‑1 (MQ=255)
                                                                                                                                         tGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTcatcat       <  1:2287300/148‑1 (MQ=255)
                                                                                                                                         tGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTcatcat       <  1:2947488/148‑1 (MQ=255)
                                                                                                                                           gggATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTg     <  2:1227538/148‑1 (MQ=255)
                                                                                                                                           gggATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTg     >  1:1444476/1‑148 (MQ=255)
                                                                                                                                            ggATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGt    <  2:1404776/148‑1 (MQ=255)
                                                                                                                                              atgctCGAACTCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTaa  <  1:1030736/148‑1 (MQ=255)
                                                                                                                                              atgctCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTaa  <  2:32992/148‑1 (MQ=255)
                                                                                                                                              atgctCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTaa  <  2:1520835/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAA  >  CP009273/4410819‑4411108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑