Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,412,786 G→T 100% E42* (GAA→TAA)  ulaE → L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,412,7860GT99.4% 544.0 / ‑4.2 167E42* (GAA→TAA) ulaEL‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (110/56);  total (111/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 167 total aligned reads displayed.

ACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACG  >  CP009273/4412641‑4412930
                                                                                                                                                 |                                                                                                                                                
aCTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTaa                                                                                                                                                >  1:3413017/1‑148 (MQ=255)
 cTGTGGGGCTAAGGCGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTaaa                                                                                                                                               <  1:656637/148‑1 (MQ=255)
  tgtgGGTCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAAc                                                                                                                                              <  2:2233778/148‑1 (MQ=255)
  tgtgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTGTGTCGAAATGTCGGTAGATTAAAc                                                                                                                                              >  1:2910661/1‑148 (MQ=255)
      gggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGAc                                                                                                                                          >  2:387821/1‑148 (MQ=255)
            aGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGc                                                                                                                                    <  1:136309/148‑1 (MQ=255)
            aGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGc                                                                                                                                    <  2:1816027/148‑1 (MQ=255)
            aGGAGCGGATATGTTGTCAAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGc                                                                                                                                    <  1:1519312/148‑1 (MQ=255)
             ggAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGcc                                                                                                                                   >  1:550222/1‑148 (MQ=255)
              gAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCt                                                                                                                                  >  2:801653/1‑148 (MQ=255)
              gAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCt                                                                                                                                  >  2:31340/1‑148 (MQ=255)
              gAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCt                                                                                                                                  >  2:2824282/1‑148 (MQ=255)
              gAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCt                                                                                                                                  >  2:2164469/1‑148 (MQ=255)
              gAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGAGCTCCCCGCCGGGGAGAGCTGGCTGGAACGCCTGCACCTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGAAGATTAAACAGACGAGCGCCt                                                                                                                                  >  1:141393/1‑148 (MQ=255)
                    atatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGTTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTcgcg                                                                                                                            >  2:106492/1‑148 (MQ=255)
                    atatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTcgcg                                                                                                                            >  1:143505/1‑148 (MQ=255)
                     tatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCgcgc                                                                                                                           <  2:2075331/148‑1 (MQ=255)
                     tatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCgcgc                                                                                                                           <  2:2075342/148‑1 (MQ=255)
                     tatGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCgcgc                                                                                                                           >  2:3072479/1‑148 (MQ=255)
                       tgttgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCt                                                                                                                         >  2:1676996/1‑148 (MQ=255)
                       tgttgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCt                                                                                                                         <  2:2995600/148‑1 (MQ=255)
                         ttgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCa                                                                                                                       >  1:1886399/1‑148 (MQ=255)
                          tgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCaa                                                                                                                      >  1:1192741/1‑148 (MQ=255)
                            tCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACt                                                                                                                    >  2:726618/1‑148 (MQ=255)
                               aaacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGa                                                                                                                 <  2:11318/148‑1 (MQ=255)
                                aacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTAGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAg                                                                                                                <  2:1856064/148‑1 (MQ=255)
                                   aaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCACCTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCcg                                                                                                             >  2:2439029/1‑148 (MQ=255)
                                    aaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCcgc                                                                                                            >  2:561287/1‑148 (MQ=255)
                                    aaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCcgc                                                                                                            >  1:3011324/1‑148 (MQ=255)
                                       cccGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                                                                                         >  1:2742673/1‑148 (MQ=255)
                                       cccGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                                                                                         >  2:273388/1‑148 (MQ=255)
                                       cccGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                                                                                         >  1:3266984/1‑148 (MQ=255)
                                        ccGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGagc                                                                                                        >  2:3154556/1‑148 (MQ=255)
                                             tGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGt                                                                                                   <  1:3480675/148‑1 (MQ=255)
                                              ggCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTc                                                                                                  <  2:1726811/148‑1 (MQ=255)
                                              ggCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTc                                                                                                  <  1:3398091/148‑1 (MQ=255)
                                               gCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCt                                                                                                 >  2:534033/1‑148 (MQ=255)
                                                   cTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcg                                                                                             >  1:1859798/1‑148 (MQ=255)
                                                     aTGAAAAAGCGCGCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCt                                                                                           <  1:1304290/148‑1 (MQ=255)
                                                      tGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGCACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTg                                                                                          <  1:961678/148‑1 (MQ=255)
                                                      tGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTg                                                                                          >  1:2374557/1‑148 (MQ=255)
                                                           aaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCGGGTCaa                                                                                     >  2:2275229/1‑148 (MQ=255)
                                                             gcgcTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGAGTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATg                                                                                   >  2:569490/1‑148 (MQ=255)
                                                                cTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGa                                                                                >  1:14193/1‑148 (MQ=255)
                                                                 tCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAt                                                                               <  2:806889/148‑1 (MQ=255)
                                                                  ccccGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAtt                                                                              >  1:813013/1‑148 (MQ=255)
                                                                  ccccGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAtt                                                                              >  2:1239112/1‑148 (MQ=255)
                                                                  ccccGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAtt                                                                              >  2:1239044/1‑148 (MQ=255)
                                                                   cccGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAttg                                                                             >  1:317847/1‑148 (MQ=255)
                                                                   cccGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAgtg                                                                             >  1:2724908/1‑148 (MQ=255)
                                                                         ggggAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGaaa                                                                       <  2:951651/148‑1 (MQ=255)
                                                                             aGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCgg                                                                   >  2:3028996/1‑148 (MQ=255)
                                                                             aGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCgg                                                                   <  1:450044/148‑1 (MQ=255)
                                                                              gTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgc                                                                  >  1:731515/1‑148 (MQ=255)
                                                                                           cGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGt                                                     <  2:1318924/148‑1 (MQ=255)
                                                                                           cGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGt                                                     >  1:3308889/1‑148 (MQ=255)
                                                                                            gCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTc                                                    >  1:1745258/1‑148 (MQ=255)
                                                                                              cTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCa                                                  <  2:2245024/148‑1 (MQ=255)
                                                                                              cTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCa                                                  >  2:1947534/1‑148 (MQ=255)
                                                                                               tGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCAt                                                 <  1:1518622/148‑1 (MQ=255)
                                                                                                     tGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCt                                           <  1:2021626/148‑1 (MQ=255)
                                                                                                      ggCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCtt                                          <  2:1632132/148‑1 (MQ=255)
                                                                                                            aaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCt                                    >  1:2621644/1‑148 (MQ=255)
                                                                                                            aaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCt                                    >  1:2621831/1‑148 (MQ=255)
                                                                                                              cGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCa                                  <  2:3174824/148‑1 (MQ=255)
                                                                                                              cGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCa                                  >  2:1001965/1‑148 (MQ=255)
                                                                                                                ttAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtc                                >  1:2182844/1‑148 (MQ=255)
                                                                                                                 tAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcg                               >  1:1460519/1‑148 (MQ=255)
                                                                                                                 tAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcg                               >  1:2714675/1‑148 (MQ=255)
                                                                                                                 tAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcg                               >  1:2706746/1‑148 (MQ=255)
                                                                                                                 tAGGCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcg                               >  1:1857765/1‑148 (MQ=255)
                                                                                                                   ggCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtc                             >  1:517730/1‑148 (MQ=255)
                                                                                                                   ggCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtc                             >  1:2009714/1‑148 (MQ=255)
                                                                                                                   ggCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtc                             >  2:1378505/1‑148 (MQ=255)
                                                                                                                   ggCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtc                             >  1:849858/1‑148 (MQ=255)
                                                                                                                    gCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtcg                            >  1:586504/1‑148 (MQ=255)
                                                                                                                    gCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtcg                            >  2:2893316/1‑148 (MQ=255)
                                                                                                                    gCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtcg                            >  2:2731817/1‑148 (MQ=255)
                                                                                                                    gCTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATAGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtcg                            >  2:422412/1‑148 (MQ=255)
                                                                                                                     cTTCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATcgtcgt                           >  2:905842/1‑148 (MQ=255)
                                                                                                                      ttCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGtt                          >  1:2271299/1‑148 (MQ=255)
                                                                                                                      ttCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGtt                          >  1:1825627/1‑148 (MQ=255)
                                                                                                                      ttCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGtt                          <  2:120373/148‑1 (MQ=255)
                                                                                                                       tCGATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGttt                         <  2:3103735/148‑1 (MQ=255)
                                                                                                                         gATTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTcc                       <  2:3360273/148‑1 (MQ=255)
                                                                                                                          aTTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTGTccc                      >  2:2146650/1‑148 (MQ=255)
                                                                                                                          aTTTTGTCGAAATGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTGTccc                      >  1:56460/1‑148 (MQ=255)
                                                                                                                           ttttGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCT‑CTCATCGTCGTTTCCCGc                    >  2:2572760/1‑148 (MQ=255)
                                                                                                                                   aaaTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAg             >  2:210228/1‑148 (MQ=255)
                                                                                                                                   aaaTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAg             >  2:1420449/1‑148 (MQ=255)
                                                                                                                                   aaaTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCGGGGCAg             >  2:210448/1‑148 (MQ=255)
                                                                                                                                    aaTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGt            <  1:1190309/148‑1 (MQ=255)
                                                                                                                                     aTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTGCCGCCGGGCCGTg           >  1:2070493/1‑148 (MQ=255)
                                                                                                                                     aTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGc               <  1:1879987/144‑1 (MQ=255)
                                                                                                                                     aTGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGc               >  2:1879987/1‑144 (MQ=255)
                                                                                                                                      tGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGccggga          >  2:1111224/1‑143 (MQ=255)
                                                                                                                                      tGTCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGa          >  2:1110859/1‑148 (MQ=255)
                                                                                                                                        tCGGTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAg        >  2:287193/1‑148 (MQ=255)
                                                                                                                                           gTAGATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATg     <  1:1603610/148‑1 (MQ=255)
                                                                                                                                              gATTAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTACCGCTGGGCCGTGAAGATGaag  >  1:1391577/1‑146 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACG  >  CP009273/4412641‑4412930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑