Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,417,122 G→T 100% R40S (CGT→AGT)  yjfZ ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,417,1220GT98.8% 738.6 / 0.3 245R40S (CGT→AGT) yjfZuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (101/139);  total (104/141)
Fisher's exact test for biased strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 246 total aligned reads displayed.

ATCCAAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAA  >  CP009273/4416978‑4417269
                                                                                                                                                |                                                                                                                                                   
 tccaAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGAttt                                                                                                                                                 >  1:2288431/1‑148 (MQ=255)
         aaTTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTaa                                                                                                                                         <  2:1670051/148‑1 (MQ=255)
         aaTTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTaa                                                                                                                                         >  1:2811459/1‑148 (MQ=255)
          aTTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAAt                                                                                                                                        <  2:273476/148‑1 (MQ=255)
           ttATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTTTAAGTAAtt                                                                                                                                       >  1:56989/1‑147 (MQ=255)
           ttATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATg                                                                                                                                       >  2:743142/1‑148 (MQ=255)
            tATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGt                                                                                                                                      <  1:1591306/148‑1 (MQ=255)
            tATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGt                                                                                                                                      >  1:1764510/1‑148 (MQ=255)
             aTCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTa                                                                                                                                     <  1:1047086/148‑1 (MQ=255)
              tCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTAAACAGTACGTCTGCCATCGCAATTATTATCTCTTACACTATTTGTAAGTAATGTAg                                                                                                                                    >  1:473775/1‑148 (MQ=255)
                 ccATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTTAAAGTAATTTAgcgc                                                                                                                                 >  2:2712769/1‑148 (MQ=255)
                 ccATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAgcgc                                                                                                                                 >  2:2047104/1‑148 (MQ=255)
                  cattcattGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGcgcg                                                                                                                                <  2:152854/148‑1 (MQ=255)
                        ttGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCgcgtgcgtt                                                                                                                          >  2:3312254/1‑147 (MQ=255)
                        ttGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTAGAAAGTAATGTAGCGCGTGCGTa                                                                                                                          >  2:755736/1‑148 (MQ=255)
                            gATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTAAGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATtga                                                                                                                      >  2:320790/1‑148 (MQ=255)
                             aTTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATtgat                                                                                                                     <  1:2391210/148‑1 (MQ=255)
                               tCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATtgatga                                                                                                                   <  1:3513277/148‑1 (MQ=255)
                                 cATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGaaa                                                                                                                 <  1:1900273/148‑1 (MQ=255)
                                  aTCGTAAGCATTACACCTCGTCGCTTACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTAAACAGTACGTCTGCCATCGCAATTATTTTCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATTAaat                                                                                                                >  2:1192980/1‑148 (MQ=255)
                                  aTCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaat                                                                                                                <  1:3120047/148‑1 (MQ=255)
                                      tAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCCTCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaatttta                                                                                                            >  2:180792/1‑148 (MQ=255)
                                       aaGCATTACACCTCGTCGCTAACTTATTGAATGCTTTCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCAACGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGGGCGTATTGATGTaattttaa                                                                                                           >  1:1013938/1‑148 (MQ=255)
                                        aGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaat                                                                                                          <  2:3205655/148‑1 (MQ=255)
                                         gCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTATTTTGTGGATCTCAACAGTACGTCTGCCATCGCAAGTAAGATCTCTTCCACTCTTTGAAAGTAATGTAGCGCGTGCGTATTGATTAaattttaatt                                                                                                         >  2:634869/1‑148 (MQ=255)
                                         gCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaatt                                                                                                         >  2:2545523/1‑148 (MQ=255)
                                         gCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGCATTGATGAaattttaatt                                                                                                         <  1:3232923/148‑1 (MQ=255)
                                           aTTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAaattttaatttt                                                                                                       <  2:1334508/148‑1 (MQ=255)
                                           aTTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGAGCGTGCGTATTGATGAaattttaatttt                                                                                                       <  2:2366388/148‑1 (MQ=255)
                                            ttACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAattttaatttta                                                                                                      >  1:865648/1‑148 (MQ=255)
                                               cacCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTg                                                                                                   <  1:3004203/148‑1 (MQ=255)
                                                acCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTgg                                                                                                  <  2:2404578/148‑1 (MQ=255)
                                                 ccTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGt                                                                                                 <  1:1463326/148‑1 (MQ=255)
                                                 ccTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGt                                                                                                 <  2:139670/148‑1 (MQ=255)
                                                  cTCGTCGCTAACTTATTTAATTCTTGCCAGTCTGAGATATCTAGTTTTTTGATCTAAAAAGTACGTATTCCATCGCAAATATTATATCTTCCACAATTTTAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTAATTGtt                                                                                                >  1:3372014/1‑148 (MQ=255)
                                                  cTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGtt                                                                                                >  2:1854390/1‑148 (MQ=255)
                                                    cgtcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTg                                                                                              >  1:3466139/1‑148 (MQ=255)
                                                      tcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTc                                                                                            <  1:3254348/148‑1 (MQ=255)
                                                      tcgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTc                                                                                            <  1:2158214/148‑1 (MQ=255)
                                                       cgCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCg                                                                                           >  2:2433893/1‑148 (MQ=255)
                                                          tAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTg                                                                                        <  2:1666289/148‑1 (MQ=255)
                                                          tAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTg                                                                                        >  2:1915187/1‑148 (MQ=255)
                                                           aaCTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTgg                                                                                       <  1:1126426/148‑1 (MQ=255)
                                                             cTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGAc                                                                                     >  1:1000320/1‑148 (MQ=255)
                                                                  tGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGaaaa                                                                                >  2:484434/1‑148 (MQ=255)
                                                                     aTGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACg                                                                             <  2:3085932/148‑1 (MQ=255)
                                                                     aTGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACg                                                                             <  1:1843839/148‑1 (MQ=255)
                                                                       gCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACgag                                                                           <  2:986055/148‑1 (MQ=255)
                                                                       gCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACgag                                                                           <  2:2626800/148‑1 (MQ=255)
                                                                       gCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACgag                                                                           >  2:434059/1‑148 (MQ=255)
                                                                       gCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACgag                                                                           <  2:986702/148‑1 (MQ=255)
                                                                       gCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACgag                                                                           <  1:1811295/148‑1 (MQ=255)
                                                                        cTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACgaga                                                                          <  2:2057808/148‑1 (MQ=255)
                                                                         tagCCCGTCAGAGAGATCAAGTTGGTCGACCTCAACAGAACGTCAGCCAGCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGAt                                                                         <  2:3404864/146‑1 (MQ=255)
                                                                          tGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATa                                                                        <  1:2603767/148‑1 (MQ=255)
                                                                          tGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATa                                                                        >  2:2573420/1‑148 (MQ=255)
                                                                            ccAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATaaa                                                                      <  2:2201547/148‑1 (MQ=255)
                                                                                tCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATg                                                                  <  1:631061/148‑1 (MQ=255)
                                                                                 cTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGc                                                                 <  1:3216234/148‑1 (MQ=255)
                                                                                   gagagaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAg                                                               >  1:717192/1‑148 (MQ=255)
                                                                                     gagaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGa                                                             >  2:446317/1‑148 (MQ=255)
                                                                                       gaTCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGaaa                                                           <  2:196222/148‑1 (MQ=255)
                                                                                         tCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATg                                                         <  2:2274499/148‑1 (MQ=255)
                                                                                           tAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaa                                                       <  2:8774/148‑1 (MQ=255)
                                                                                             gTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaata                                                     <  2:3485754/148‑1 (MQ=255)
                                                                                              tttGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGaataa                                                    >  1:399906/1‑148 (MQ=255)
                                                                                                     aTCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCg                                             <  2:90750/148‑1 (MQ=255)
                                                                                                        tcAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTg                                          <  2:3295045/148‑1 (MQ=255)
                                                                                                           aCAGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTa                                       <  1:2129592/148‑1 (MQ=255)
                                                                                                             aGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAAt                                     >  1:209409/1‑148 (MQ=255)
                                                                                                             aGTACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAAt                                     >  2:790480/1‑148 (MQ=255)
                                                                                                               tACGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGt                                   <  1:1449345/148‑1 (MQ=255)
                                                                                                                aCGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTc                                  <  2:2116839/148‑1 (MQ=255)
                                                                                                                 cGTCTGCCATCGCAATTATGATCTCTTCCACTATTTGAATGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCa                                 >  2:1293748/1‑148 (MQ=255)
                                                                                                                   tCTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCAtt                               >  1:1947168/1‑148 (MQ=255)
                                                                                                                    cTGCCATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTg                              >  2:302920/1‑148 (MQ=255)
                                                                                                                       ccATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTaa                           <  1:2963352/148‑1 (MQ=255)
                                                                                                                       ccATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTaa                           >  2:2771582/1‑148 (MQ=255)
                                                                                                                        cATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAAt                          <  2:2019360/148‑1 (MQ=255)
                                                                                                                        cATCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAAt                          >  1:281072/1‑148 (MQ=255)
                                                                                                                         aTCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATg                         <  2:1571135/148‑1 (MQ=255)
                                                                                                                         aTCGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATg                         <  2:1480589/148‑1 (MQ=255)
                                                                                                                           cGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGtt                       <  1:743142/148‑1 (MQ=255)
                                                                                                                           cGCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGtt                       <  1:1556318/148‑1 (MQ=255)
                                                                                                                            gCAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTa                      <  1:2545523/148‑1 (MQ=255)
                                                                                                                            gCAATAATTATCTCTTCCACTATTAGAAAGTAATGAAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGAGAAATATGCAGGAAATGAATACATAGTCGTTGGTAATGTCATTGTAATGTTa                      >  2:2685622/1‑148 (MQ=255)
                                                                                                                             cAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTTGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTAc                     <  1:2573420/148‑1 (MQ=255)
                                                                                                                             cAATTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTAc                     <  2:2811459/148‑1 (MQ=255)
                                                                                                                               aTTATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCt                   >  1:2851991/1‑148 (MQ=255)
                                                                                                                                ttATGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTg                  >  1:2832346/1‑148 (MQ=255)
                                                                                                                                  aTGATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACTAAAAACGATATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGtt                >  2:2961287/1‑148 (MQ=255)
                                                                                                                                    gATCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTa              <  2:1750912/148‑1 (MQ=255)
                                                                                                                                     aTCTCTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTaa             >  1:1197184/1‑148 (MQ=255)
                                                                                                                                      tctcTTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTGTAAt            >  2:827241/1‑148 (MQ=255)
                                                                                                                                       ctctTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATg           >  1:1840844/1‑148 (MQ=255)
                                                                                                                                        tctTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGt          <  1:2831138/148‑1 (MQ=255)
                                                                                                                                         ctTCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGtt         <  1:2712769/148‑1 (MQ=255)
                                                                                                                                          ttCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGttt        <  2:621026/148‑1 (MQ=255)
                                                                                                                                          ttCCACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGttt        >  2:3145180/1‑148 (MQ=255)
                                                                                                                                             cACTATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTta     <  2:1049289/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ATCCAAAAGAATTATCACCATTCATTGTGATTCCATCGTAAGCATTACACCTCGTCGCTAACTTATTGAATGCTTGCCAGTCTGAGAGATCTAGTTTGTGGATCTCAACAGTACGTCTGCCATCGCAATTATGATCTCTTCCACGATTTGAAAGTAATGTAGCGCGTGCGTATTGATGAAATTTTAATTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAA  >  CP009273/4416978‑4417269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑