Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,445,719 A→T 100% T40S (ACC→TCC)  mpl → UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,445,7190AT97.0% 767.1 / 9.0 236T40S (ACC→TCC) mplUDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base A (3/4);  new base T (93/136);  total (96/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 236 total aligned reads displayed.

GCGGCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCACCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATCCCTT  >  CP009273/4445574‑4445863
                                                                                                                                                 |                                                                                                                                                
 cGGCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCt                                                                                                                                               >  1:2404303/1‑148 (MQ=255)
 cGGCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCt                                                                                                                                               >  2:2737563/1‑148 (MQ=255)
   gCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTa                                                                                                                                             <  2:2986587/148‑1 (MQ=255)
    cAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTAc                                                                                                                                            <  1:155621/148‑1 (MQ=255)
             aaaGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTCGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGc                                                                                                                                   >  2:1746252/1‑148 (MQ=255)
              aaGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCa                                                                                                                                  <  2:703308/148‑1 (MQ=255)
               aGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCaa                                                                                                                                 >  1:3055009/1‑148 (MQ=255)
               aGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCaa                                                                                                                                 <  2:2263855/148‑1 (MQ=255)
                ggATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAg                                                                                                                                >  2:1677480/1‑148 (MQ=255)
                ggATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCACCTTACTTGAGAAGCAAg                                                                                                                                >  1:2254135/1‑148 (MQ=255)
                ggATAGGGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAg                                                                                                                                <  1:2833877/148‑1 (MQ=255)
                 gATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAgg                                                                                                                               >  2:1869255/1‑148 (MQ=255)
                  aTAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGc                                                                                                                              <  1:2338658/148‑1 (MQ=255)
                        aCGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAg                                                                                                                        >  2:2379805/1‑148 (MQ=255)
                        aCGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAg                                                                                                                        <  2:711329/148‑1 (MQ=255)
                        aCGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAg                                                                                                                        <  1:1434260/148‑1 (MQ=255)
                         cGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGc                                                                                                                       <  1:1406571/148‑1 (MQ=255)
                                   ttagtatTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTATGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAggg                                                                                                             <  1:2652502/144‑1 (MQ=255)
                                    tCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAgggg                                                                                                            <  1:2787189/148‑1 (MQ=255)
                                     cATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGt                                                                                                           <  1:1909567/148‑1 (MQ=255)
                                      atatTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTa                                                                                                          <  1:659607/148‑1 (MQ=255)
                                        atTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACg                                                                                                        <  2:1977474/148‑1 (MQ=255)
                                         ttttAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGa                                                                                                       >  2:1898823/1‑148 (MQ=255)
                                         ttttAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGa                                                                                                       >  2:2648299/1‑148 (MQ=255)
                                           ttAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATg                                                                                                     <  1:1608284/148‑1 (MQ=255)
                                            tAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATgc                                                                                                    >  1:597587/1‑148 (MQ=255)
                                            tAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATgc                                                                                                    <  1:1366740/148‑1 (MQ=255)
                                              ggAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATgcca                                                                                                  >  1:3401991/1‑148 (MQ=255)
                                                aaTTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATgccagc                                                                                                <  2:473762/148‑1 (MQ=255)
                                                aaTTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCACCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATgccagc                                                                                                <  2:2254135/148‑1 (MQ=255)
                                                 aTTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATgccagcc                                                                                               <  1:293583/148‑1 (MQ=255)
                                                  tttGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCCATGTGTATCCGCCGATGAGCTCCTTACTTGTGAAGCAAGGCATTGAGCTGATTCTGGGGTACGATgccagcca                                                                                              >  2:2973693/1‑148 (MQ=255)
                                                   ttGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCGTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGccagccag                                                                                             <  2:2790951/148‑1 (MQ=255)
                                                    tgtgGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCcagccagc                                                                                            <  1:3268653/148‑1 (MQ=255)
                                                      tgGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTc                                                                                          >  1:1810374/1‑148 (MQ=255)
                                                            atgtATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCg                                                                                    <  2:359908/145‑1 (MQ=255)
                                                            aTTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCg                                                                                    <  2:2276797/148‑1 (MQ=255)
                                                            aTTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCg                                                                                    <  2:512386/148‑1 (MQ=255)
                                                            aTTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCg                                                                                    >  1:2190943/1‑148 (MQ=255)
                                                             tttATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGc                                                                                   <  2:678085/148‑1 (MQ=255)
                                                             tttATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGc                                                                                   >  2:3478501/1‑148 (MQ=255)
                                                                aTGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGc                                                                                <  1:1624050/148‑1 (MQ=255)
                                                                  gggCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCg                                                                              >  2:864547/1‑148 (MQ=255)
                                                                    gCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGa                                                                            <  2:2544697/148‑1 (MQ=255)
                                                                     cGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGAt                                                                           <  2:2417272/148‑1 (MQ=255)
                                                                     cGGTCTGGCGATGCTGGAGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGAt                                                                           <  1:1802979/148‑1 (MQ=255)
                                                                        tCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTg                                                                        >  1:1153933/1‑148 (MQ=255)
                                                                         cTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTgg                                                                       <  2:2193724/148‑1 (MQ=255)
                                                                         cTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTgg                                                                       <  2:763000/148‑1 (MQ=255)
                                                                           ggCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTg                                                                     <  2:1316756/148‑1 (MQ=255)
                                                                            gCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGa                                                                    <  1:3162977/148‑1 (MQ=255)
                                                                             cGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGat                                                                   <  2:350371/148‑1 (MQ=255)
                                                                              gATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGatt                                                                  <  2:1651602/148‑1 (MQ=255)
                                                                                  cTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTg                                                              <  1:1752461/148‑1 (MQ=255)
                                                                                     gcgcgcCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCa                                                           <  1:1822019/148‑1 (MQ=255)
                                                                                     gcgcgcCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCa                                                           <  1:1079125/148‑1 (MQ=255)
                                                                                        cgcCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACg                                                        <  1:1313235/148‑1 (MQ=255)
                                                                                             gTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATg                                                   >  1:2652915/1‑148 (MQ=255)
                                                                                              ttAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGa                                                  >  2:314420/1‑148 (MQ=255)
                                                                                                  gCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCg                                              >  1:220152/1‑148 (MQ=255)
                                                                                                   ccATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGt                                             <  2:649940/148‑1 (MQ=255)
                                                                                                   ccATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGt                                             <  1:746960/148‑1 (MQ=255)
                                                                                                     aTGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTgg                                           <  1:2872284/148‑1 (MQ=255)
                                                                                                       gAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGaa                                         <  1:2143585/148‑1 (MQ=255)
                                                                                                         aGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAAt                                       <  2:2241161/148‑1 (MQ=255)
                                                                                                         aGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCACCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAAt                                       <  1:3321521/148‑1 (MQ=255)
                                                                                                          gTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATc                                      <  1:160264/148‑1 (MQ=255)
                                                                                                          gTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATc                                      >  2:848068/1‑148 (MQ=255)
                                                                                                          gTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATc                                      >  2:2605640/1‑148 (MQ=255)
                                                                                                          gTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATc                                      <  1:793512/148‑1 (MQ=255)
                                                                                                            aaCGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCg                                    <  1:1086483/148‑1 (MQ=255)
                                                                                                            aaCGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCg                                    <  1:1677480/148‑1 (MQ=255)
                                                                                                            aaCGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCg                                    >  1:2681216/1‑148 (MQ=255)
                                                                                                            aaCGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCg                                    >  1:2267536/1‑148 (MQ=255)
                                                                                                                ggTTCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCgtgtg                                <  1:2033498/148‑1 (MQ=255)
                                                                                                                   tCGGACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTgg                             <  1:243126/148‑1 (MQ=255)
                                                                                                                     ggACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGaa                           >  2:584972/1‑148 (MQ=255)
                                                                                                                      gACGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAg                          <  1:386776/148‑1 (MQ=255)
                                                                                                                        cGCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCg                        <  2:1802335/148‑1 (MQ=255)
                                                                                                                         gCCAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCgg                       <  2:3419224/148‑1 (MQ=255)
                                                                                                                          ccAATGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGt                      >  1:1653326/1‑148 (MQ=255)
                                                                                                                            aaTGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCgtgtg                                >  1:1482680/1‑136 (MQ=255)
                                                                                                                            aaTGTGTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCgtgtg                                <  2:1482680/136‑1 (MQ=255)
                                                                                                                              tgtgTATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTg                  <  2:1046022/148‑1 (MQ=255)
                                                                                                                                tgtATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGa                >  1:3027456/1‑148 (MQ=255)
                                                                                                                                  tATCCGCCGATTAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCTGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGata              <  2:1718780/148‑3 (MQ=255)
                                                                                                                                  tATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGaaa              <  2:2512167/148‑1 (MQ=255)
                                                                                                                                  tATCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGaaa              <  2:476114/148‑1 (MQ=255)
                                                                                                                                    tCCGCCGATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGaaaaa            <  2:3420798/148‑1 (MQ=255)
                                                                                                                                     ccgccgATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGaaaaaa           <  2:3298423/148‑1 (MQ=255)
                                                                                                                                     ccgccgATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGaaaaaa           <  1:2538108/148‑1 (MQ=255)
                                                                                                                                     ccgccgATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGaaaaaa           <  2:1555120/148‑1 (MQ=255)
                                                                                                                                       gccgATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAat         <  1:2910087/148‑1 (MQ=255)
                                                                                                                                          gATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATc      <  1:519714/148‑1 (MQ=255)
                                                                                                                                          gATGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATc      >  1:764304/1‑148 (MQ=255)
                                                                                                                                           aTGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATcc     <  2:3269039/148‑1 (MQ=255)
                                                                                                                                           aTGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATcc     >  2:101644/1‑148 (MQ=255)
                                                                                                                                           aTGAGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATcc     <  2:2596145/148‑1 (MQ=255)
                                                                                                                                              aGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATCCCtt  <  2:2925527/148‑1 (MQ=255)
                                                                                                                                              aGCTCCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATCCCtt  <  2:2734936/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCGGCAGATTAAAAAAGGATAGTGACGTATGCGCATTCATATTTTAGGAATTTGTGGCACATTTATGGGCGGTCTGGCGATGCTGGCGCGCCAGTTAGGCCATGAAGTAACGGGTTCGGACGCCAATGTGTATCCGCCGATGAGCACCTTACTTGAGAAGCAAGGCATTGAGCTGATTCAGGGGTACGATGCCAGCCAGCTCGAGCCGCAGCCGGATCTGGTGATTATTGGCAACGCCATGACCCGTGGAAATCCGTGTGTGGAAGCGGTACTGGAAAAAAATATCCCTT  >  CP009273/4445574‑4445863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑