Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,470,873 G→T 100% R928S (CGT→AGT)  valS ← valyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,470,8730GT98.5% 544.1 / ‑2.8 178R928S (CGT→AGT) valSvalyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base T (92/81);  total (96/82)
Fisher's exact test for biased strand distribution p-value = 3.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 182 total aligned reads displayed.

TTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGGCCAGC  >  CP009273/4470728‑4471017
                                                                                                                                                 |                                                                                                                                                
tttACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCActct                                                                                                                                                >  1:2210739/1‑148 (MQ=255)
 ttACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATATCCTTACAGCTTCTCACtctc                                                                                                                                               >  1:1511169/1‑148 (MQ=255)
 ttACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACtctc                                                                                                                                               >  1:726677/1‑148 (MQ=255)
    ccATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCCTAGCCTTCCAGCTTCTCACTCTCttt                                                                                                                                            >  2:2601390/1‑148 (MQ=255)
        ttCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTcgcg                                                                                                                                        <  2:697589/148‑1 (MQ=255)
          cTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGAt                                                                                                                                      <  2:3046038/148‑1 (MQ=255)
                gagaTAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGc                                                                                                                                >  2:443052/1‑148 (MQ=255)
                 agaTAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCt                                                                                                                               >  1:2487936/1‑148 (MQ=255)
                   ataataAAGGCCGGAGCATGCTCCGGCCTTCGGTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCGTCCGCATAGCCTTCCAGCTTCTCACTCGCTTTCGCGATGACCGCTTc                                                                                                                             >  2:2707090/1‑148 (MQ=255)
                   aTAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTc                                                                                                                             >  2:1921507/1‑148 (MQ=255)
                    tAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTcc                                                                                                                            >  1:2740630/1‑148 (MQ=255)
                    tAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTcc                                                                                                                            >  1:3086398/1‑148 (MQ=255)
                    tAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTcc                                                                                                                            >  1:1923191/1‑148 (MQ=255)
                        aaaGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTg                                                                                                                        <  2:878479/148‑1 (MQ=255)
                         aaGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTGGCTTCCGCATAGCCTTCCAGCGTCTCACGCTCTTTGGGGGTGACCGGATCCGGTgg                                                                                                                       >  1:3257035/1‑147 (MQ=255)
                          aGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTgcg                                                                                                                      >  2:3476682/1‑148 (MQ=255)
                            gCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTAGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGcgcg                                                                                                                    >  2:1502404/1‑148 (MQ=255)
                                gAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCg                                                                                                                >  1:1509852/1‑148 (MQ=255)
                                 aGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGa                                                                                                               >  2:3346971/1‑148 (MQ=255)
                                     tGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACaaa                                                                                                           >  1:432878/1‑148 (MQ=255)
                                     tGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCAAAGCCTTCCAGCTTCTCACTCTCTTTCGCGCTGACCGCTTCCGGTGCGCGGGCGCCaaa                                                                                                           >  1:343767/1‑148 (MQ=255)
                                     tGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTACGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACaaa                                                                                                           >  1:2143232/1‑148 (MQ=255)
                                      gCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAg                                                                                                          >  1:3167417/1‑148 (MQ=255)
                                      gCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAg                                                                                                          >  2:2994214/1‑148 (MQ=255)
                                        tCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGcc                                                                                                        <  1:2584333/148‑1 (MQ=255)
                                           ggCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttc                                                                                                     <  2:1291877/148‑1 (MQ=255)
                                             ccTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcgt                                                                                                   <  1:1017413/148‑1 (MQ=255)
                                               ttCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcgttc                                                                                                 >  2:2031145/1‑148 (MQ=255)
                                                tCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcgttcg                                                                                                >  2:3295848/1‑148 (MQ=255)
                                                tCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCttcgttcg                                                                                                >  1:1182934/1‑148 (MQ=255)
                                                   ttttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCa                                                                                             >  2:1812196/1‑148 (MQ=255)
                                                   ttttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCa                                                                                             >  1:1289380/1‑148 (MQ=255)
                                                   ttttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCa                                                                                             <  2:2516851/148‑1 (MQ=255)
                                                    tttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAg                                                                                            >  1:1461942/1‑148 (MQ=255)
                                                     ttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGt                                                                                           >  1:1167800/1‑148 (MQ=255)
                                                     ttCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGAGGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGt                                                                                           <  2:1390915/148‑1 (MQ=255)
                                                      tcatcaCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCGTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGtt                                                                                          <  2:236290/148‑1 (MQ=255)
                                                       catcaCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGttt                                                                                         >  1:1425516/1‑148 (MQ=255)
                                                        atcaCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTg                                                                                        <  2:1214847/148‑1 (MQ=255)
                                                          caCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCGTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGAGGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGtt                                                                                      <  2:2602409/148‑1 (MQ=255)
                                                            cTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTtct                                                                                    >  1:1857703/1‑148 (MQ=255)
                                                            cTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTtct                                                                                    <  2:501219/148‑1 (MQ=255)
                                                            cTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTtct                                                                                    <  2:2803318/148‑1 (MQ=255)
                                                              gtgtTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCg                                                                                  <  1:2714666/148‑1 (MQ=255)
                                                                gtTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGAt                                                                                <  1:459733/148‑1 (MQ=255)
                                                                 ttttGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATa                                                                               <  2:2487936/148‑1 (MQ=255)
                                                                  tttGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATAc                                                                              >  1:287926/1‑148 (MQ=255)
                                                                  tttGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATAc                                                                              >  1:2262502/1‑148 (MQ=255)
                                                                     gATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGc                                                                           <  2:2972128/148‑1 (MQ=255)
                                                                     gATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGc                                                                           <  2:382709/148‑1 (MQ=255)
                                                                      aTTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCt                                                                          <  1:2970472/148‑1 (MQ=255)
                                                                      aTTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCt                                                                          <  1:3472295/148‑1 (MQ=255)
                                                                      aTTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCt                                                                          >  2:1316630/1‑148 (MQ=255)
                                                                        tACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGCCAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGa                                                                        >  1:13404/1‑148 (MQ=255)
                                                                        tACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGa                                                                        >  2:1824420/1‑148 (MQ=255)
                                                                             cgcgGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCa                                                                   >  2:917918/1‑148 (MQ=255)
                                                                              gcggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCAc                                                                  <  1:1921507/148‑1 (MQ=255)
                                                                               cggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCAcc                                                                 <  1:1750683/148‑1 (MQ=255)
                                                                                ggcgATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCt                                                                <  1:1019054/148‑1 (MQ=255)
                                                                                     ttaCAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAAt                                                           <  1:511859/146‑1 (MQ=255)
                                                                                     tAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAAt                                                           <  2:1900732/148‑1 (MQ=255)
                                                                                     tAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAAt                                                           >  2:1909690/1‑148 (MQ=255)
                                                                                        cAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCtt                                                        >  1:2413139/1‑148 (MQ=255)
                                                                                            ctgctgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGcc                                                    <  2:1980099/148‑1 (MQ=255)
                                                                                              gctgTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCAc                                                  <  1:1054328/148‑1 (MQ=255)
                                                                                                   tCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCActtctt                                             <  2:2644303/148‑1 (MQ=255)
                                                                                                    cAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCttt                                            <  2:1656911/148‑1 (MQ=255)
                                                                                                    cAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCttt                                            <  2:276975/148‑1 (MQ=255)
                                                                                                      aTCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCg                                          <  1:3433207/148‑1 (MQ=255)
                                                                                                      aTCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTAGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCg                                          >  2:2404150/1‑148 (MQ=255)
                                                                                                       tCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGc                                         <  1:827056/148‑1 (MQ=255)
                                                                                                            ttcgctttcgctTCCGCATAGCCTTCAAGCTTCTAACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGAc                                    >  2:1321830/1‑148 (MQ=255)
                                                                                                                 tttcgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGcc                               >  2:2409791/1‑148 (MQ=255)
                                                                                                                 tttcgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGcc                               >  2:647774/1‑148 (MQ=255)
                                                                                                                    cgcttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGc                            <  2:726677/148‑1 (MQ=255)
                                                                                                                       ttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCa                         >  1:2849868/1‑148 (MQ=255)
                                                                                                                       ttCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCa                         >  1:2849844/1‑148 (MQ=255)
                                                                                                                        tCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAt                        <  2:852386/148‑1 (MQ=255)
                                                                                                                        tCCGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAt                        <  2:1859673/148‑1 (MQ=255)
                                                                                                                         ccGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtc                       >  2:85398/1‑148 (MQ=255)
                                                                                                                         ccGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtc                       >  1:1707243/1‑148 (MQ=255)
                                                                                                                         ccGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtc                       >  1:2909148/1‑148 (MQ=255)
                                                                                                                         ccGCATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtc                       >  2:2631682/1‑148 (MQ=255)
                                                                                                                         ccGCATAGCCTTCCAGCTTCTCACTCTCTTGCGCGAGGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtc                       <  2:1114203/148‑1 (MQ=255)
                                                                                                                            cATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtcttc                    >  2:1582205/1‑148 (MQ=255)
                                                                                                                            cATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtcttc                    >  2:2099103/1‑148 (MQ=255)
                                                                                                                            cATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtcttc                    <  1:1317316/148‑1 (MQ=255)
                                                                                                                            cATAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtcttc                    >  2:2649660/1‑148 (MQ=255)
                                                                                                                             aTAGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCAtcttct                   <  2:2740630/148‑1 (MQ=255)
                                                                                                                               aGCCTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTACGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCttt                 >  2:2285309/1‑148 (MQ=255)
                                                                                                                                 ccTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGGGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttgt               <  2:2210739/148‑1 (MQ=255)
                                                                                                                                  cTTCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttgtt              >  2:1555170/1‑148 (MQ=255)
                                                                                                                                   ttCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTttgttg             >  1:1255287/1‑148 (MQ=255)
                                                                                                                                    tCCAGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtga            >  1:3016509/1‑148 (MQ=255)
                                                                                                                                       aGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtgatga         <  1:2031145/148‑1 (MQ=255)
                                                                                                                                       aGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtgatga         >  1:701759/1‑148 (MQ=255)
                                                                                                                                       aGCTTCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTtgatga         >  2:773855/1‑148 (MQ=255)
                                                                                                                                          ttCTCACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgc      <  1:881635/148‑1 (MQ=255)
                                                                                                                                           tctcACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgcc     <  1:3333827/148‑1 (MQ=255)
                                                                                                                                              cACTCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGgccagc  >  1:3106965/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTTACCATTTCTGTAAGAGATAAAAAAGGCCGGAGCATGCTCCGGCCTTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCTCACGCTCTTTCGCGATGACCGCTTCCGGTGCGCGGGCGACAAAGCCTTCGTTCGCCAGTTTGTTCTCGATACGGCTGATTTCACCTTCAATCTTCGCCACTTCTTTCGCCAGACGCGCCAGCTCATCTTCTTTGTTGATGAGGCCAGC  >  CP009273/4470728‑4471017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑