Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,471,930 T→A 100% P575P (CCA→CCT)  valS ← valyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,471,9300TA99.4% 585.2 / ‑4.5 177P575P (CCA→CCT) valSvalyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (86/90);  total (87/90)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 177 total aligned reads displayed.

TAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTG  >  CP009273/4471785‑4472074
                                                                                                                                                 |                                                                                                                                                
tAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAgg                                                                                                                                                >  1:3073374/1‑148 (MQ=255)
  gACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCa                                                                                                                                              >  2:2543310/1‑148 (MQ=255)
   aCGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAg                                                                                                                                             <  2:2389568/148‑1 (MQ=255)
   aCGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAg                                                                                                                                             <  2:2389589/148‑1 (MQ=255)
          cgccgcCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATa                                                                                                                                      >  1:3176856/1‑148 (MQ=255)
                   ggTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAAcc                                                                                                                             <  1:2452928/148‑1 (MQ=255)
                   ggTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAAcc                                                                                                                             >  2:770855/1‑148 (MQ=255)
                    gTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCa                                                                                                                            <  1:568102/148‑1 (MQ=255)
                      gAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCata                                                                                                                          <  1:489835/148‑1 (MQ=255)
                      gAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCata                                                                                                                          <  1:3479486/148‑1 (MQ=255)
                      gAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCata                                                                                                                          <  1:2115630/148‑1 (MQ=255)
                           gcagcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAg                                                                                                                     <  2:2665552/148‑1 (MQ=255)
                           gcagcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAg                                                                                                                     <  2:279039/148‑1 (MQ=255)
                            cagcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGt                                                                                                                    >  2:1258255/1‑148 (MQ=255)
                             agcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTg                                                                                                                   <  2:2079013/148‑1 (MQ=255)
                              gcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTgg                                                                                                                  >  2:259895/1‑148 (MQ=255)
                              gcgcGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTgg                                                                                                                  >  2:2033106/1‑148 (MQ=255)
                               cgcgTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTggg                                                                                                                 <  2:40381/148‑1 (MQ=255)
                               cgcgTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTggg                                                                                                                 >  2:2346271/1‑148 (MQ=255)
                                gcgTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGt                                                                                                                <  2:2031314/148‑1 (MQ=255)
                                     aGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATa                                                                                                           <  2:21334/148‑1 (MQ=255)
                                         ccGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTCCGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGt                                                                                                       >  1:1742333/1‑148 (MQ=255)
                                           gTGCGGCTCAATACCGTTCGGTAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACTTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACAATATCCAGTGGGTCGATAACGTTa                                                                                                     <  2:1021053/148‑1 (MQ=255)
                                           gTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTa                                                                                                     <  2:1431508/148‑1 (MQ=255)
                                           gTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTa                                                                                                     <  2:350877/148‑1 (MQ=255)
                                           gTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTa                                                                                                     <  2:3239909/148‑1 (MQ=255)
                                           gTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTa                                                                                                     <  2:3179095/148‑1 (MQ=255)
                                            tGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTAc                                                                                                    >  2:2380081/1‑148 (MQ=255)
                                             gCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTAcc                                                                                                   >  1:2742874/1‑148 (MQ=255)
                                             gCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTAcc                                                                                                   >  1:2742878/1‑148 (MQ=255)
                                                gCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCtt                                                                                                <  1:3070743/148‑1 (MQ=255)
                                                   cAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGa                                                                                             <  1:2390253/148‑1 (MQ=255)
                                                   cAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGa                                                                                             <  2:3202339/148‑1 (MQ=255)
                                                   cAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGa                                                                                             <  1:2084869/148‑1 (MQ=255)
                                                     aTACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGAtt                                                                                           <  2:3081120/148‑1 (MQ=255)
                                                     aTACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGAtt                                                                                           <  1:1040845/148‑1 (MQ=255)
                                                      tACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGAttt                                                                                          >  2:3223784/1‑148 (MQ=255)
                                                      tACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGAttt                                                                                          >  2:3223796/1‑148 (MQ=255)
                                                       aCCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTg                                                                                         >  2:155862/1‑148 (MQ=255)
                                                       aCCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTg                                                                                         >  2:145574/1‑148 (MQ=255)
                                                       aCCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTg                                                                                         >  1:2646398/1‑148 (MQ=255)
                                                        ccGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTgg                                                                                        <  2:1051095/148‑1 (MQ=255)
                                                             cGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtc                                                                                   <  2:2809534/148‑1 (MQ=255)
                                                              gggAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtct                                                                                  <  2:1738649/148‑1 (MQ=255)
                                                              gggAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtct                                                                                  <  2:1666193/148‑1 (MQ=255)
                                                              gggAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtct                                                                                  <  2:1631820/148‑1 (MQ=255)
                                                              gggAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtct                                                                                  <  2:307932/148‑1 (MQ=255)
                                                               ggAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtctt                                                                                 >  1:2285909/1‑148 (MQ=255)
                                                               ggAACTGCGTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACAtctt                                                                                 <  2:1858832/148‑1 (MQ=255)
                                                                     gCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGcc                                                                           >  1:1299330/1‑148 (MQ=255)
                                                                        tctcGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTc                                                                        >  1:3198359/1‑148 (MQ=255)
                                                                          tcGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGt                                                                      <  1:2199606/148‑1 (MQ=255)
                                                                            ggTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGtca                                                                    <  1:1491496/148‑1 (MQ=255)
                                                                                   ttACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGaa                                                             >  2:3077182/1‑148 (MQ=255)
                                                                                    tACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAAt                                                            >  2:1477530/1‑148 (MQ=255)
                                                                                    tACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAAt                                                            >  2:1960072/1‑148 (MQ=255)
                                                                                     aCGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATc                                                           <  1:729840/148‑1 (MQ=255)
                                                                                       ggATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAg                                                         >  2:1688858/1‑148 (MQ=255)
                                                                                         aTTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGc                                                       >  2:2277435/1‑148 (MQ=255)
                                                                                          ttttGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGcc                                                      >  1:1546329/1‑148 (MQ=255)
                                                                                          ttttGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGcc                                                      >  1:1452604/1‑148 (MQ=255)
                                                                                          ttttGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGcc                                                      >  2:3029075/1‑148 (MQ=255)
                                                                                           tttGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCg                                                     >  2:726145/1‑148 (MQ=255)
                                                                                            ttGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCgg                                                    <  2:912985/148‑1 (MQ=255)
                                                                                               tCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCa                                                 <  2:1221730/148‑1 (MQ=255)
                                                                                               tCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCa                                                 >  1:752164/1‑148 (MQ=255)
                                                                                                 cGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATg                                               <  1:754168/148‑1 (MQ=255)
                                                                                                   ccAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTa                                             >  1:1524555/1‑148 (MQ=255)
                                                                                                    cAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTaa                                            >  2:2078742/1‑148 (MQ=255)
                                                                                                    cAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTaa                                            >  1:1812453/1‑148 (MQ=255)
                                                                                                    cAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTaa                                            >  1:1810093/1‑148 (MQ=255)
                                                                                                      gCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATAACCAGTGGGTCGATAACGTTACCCTTGGATTTTGACATCTTCTGTCCTTCGTCATCAAGAATCAGGCCGGTCATGTaaaa                                          >  1:911692/1‑147 (MQ=255)
                                                                                                        tGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACgg                                        <  1:2006465/148‑1 (MQ=255)
                                                                                                        tGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACgg                                        <  1:2887778/148‑1 (MQ=255)
                                                                                                          cGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGgtg                                      >  2:1160388/1‑148 (MQ=255)
                                                                                                          cGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGgtg                                      <  1:1684466/148‑1 (MQ=255)
                                                                                                               gCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGaa                                 <  1:2653864/148‑1 (MQ=255)
                                                                                                               gCATCATATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGaa                                 >  1:2902402/1‑148 (MQ=255)
                                                                                                                  tcatATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACgg                              >  2:2635642/1‑148 (MQ=255)
                                                                                                                   catATTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGc                             >  1:1623016/1‑148 (MQ=255)
                                                                                                                    atatTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCa                            >  1:1512220/1‑148 (MQ=255)
                                                                                                                      atTGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCAcc                          >  1:1895933/1‑148 (MQ=255)
                                                                                                                       gtGCCGGTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCt                         <  1:695334/147‑1 (MQ=255)
                                                                                                                             gTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGtt                   <  1:1572834/148‑1 (MQ=255)
                                                                                                                             gTACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGtt                   >  1:2557732/1‑148 (MQ=255)
                                                                                                                              tACGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGttt                  >  2:2562917/1‑148 (MQ=255)
                                                                                                                               aCGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTg                 <  2:1074724/148‑1 (MQ=255)
                                                                                                                                cGTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGc                >  1:1488794/1‑148 (MQ=255)
                                                                                                                                 gTTTTTCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGcc               <  1:877288/148‑1 (MQ=255)
                                                                                                                                   ttttCCCTCAGTTCAGGCAGCGAAATACCGTCAAACATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCTCGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAt             <  2:2917818/148‑1 (MQ=255)
                                                                                                                                   ttttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCAt             <  2:2917831/148‑1 (MQ=255)
                                                                                                                                   ttttCCAGCAGTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGAAAAAGGTGTGGACCGGCACCTGCGGTTTGCCAt             >  2:231105/1‑148 (MQ=255)
                                                                                                                                          gcagTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATc      <  2:3161624/148‑1 (MQ=255)
                                                                                                                                           cagTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCt     <  2:176032/148‑1 (MQ=255)
                                                                                                                                           cagTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCt     >  1:1034629/1‑148 (MQ=255)
                                                                                                                                             gTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCttt   <  1:3223731/148‑1 (MQ=255)
                                                                                                                                             gTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCttt   <  1:3223784/148‑1 (MQ=255)
                                                                                                                                             gTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCttt   <  1:3224270/148‑1 (MQ=255)
                                                                                                                                             gTTCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCttt   >  2:498979/1‑148 (MQ=255)
                                                                                                                                              ttCAGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTtg  >  2:2392603/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAGACGCCAGCGCCGCCAGGGTGAAGCGCAGCGCGTCAGTACCGTGCGGCTCAATACCGTTCGGGAACTGCTTCTCGGTGCGCTTACGGATTTTGTCCGCCAGCTGCGGCTGCATCATATTGCCGGTACGTTTTTCCAGCAGTTCTGGCAGCGAAATACCGTCAACCATATCCAGTGGGTCGATAACGTTACCCTTGGATTTGGACATCTTCTGGCCTTCGTCATCACGAATCAGGCCGGTCATGTAAACGGTGTGGAACGGCACCTGCGGTTTGCCATTTTCATCTTTG  >  CP009273/4471785‑4472074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑