Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,512,693 T→A 100% K407* (AAG→TAG)  yjhG ← putative dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,512,6930TA99.5% 620.9 / ‑4.5 188K407* (AAG→TAG) yjhGputative dehydratase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (96/91);  total (97/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 188 total aligned reads displayed.

GCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGC  >  CP009273/4512548‑4512837
                                                                                                                                                 |                                                                                                                                                
gCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGa                                                                                                                                                <  1:1676119/148‑1 (MQ=255)
    tGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCg                                                                                                                                            <  2:2029038/148‑1 (MQ=255)
       aTTTGATCACCGAACCTTCTTGCGCAATATTGCCCACCTGGAAGGTGATAGTTGAGTTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTg                                                                                                                                         <  1:1014700/148‑1 (MQ=255)
         ttGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGAc                                                                                                                                       >  1:1222046/1‑148 (MQ=255)
         ttGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGAc                                                                                                                                       >  2:2021134/1‑148 (MQ=255)
           gATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGc                                                                                                                                     >  1:2942914/1‑148 (MQ=255)
                         cTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTc                                                                                                                       <  2:745565/148‑1 (MQ=255)
                          tGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTTCTAGAACCGCTGACGCCGTTCGGAGTGCTcc                                                                                                                      <  2:967170/148‑1 (MQ=255)
                           ggCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTccc                                                                                                                     <  2:1510125/148‑1 (MQ=255)
                            gcgcAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCcca                                                                                                                    >  1:3327962/1‑148 (MQ=255)
                             cgcAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCccac                                                                                                                   >  1:3410534/1‑148 (MQ=255)
                                aaTATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAg                                                                                                                >  1:1310038/1‑148 (MQ=255)
                                   atTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCg                                                                                                             <  2:1367468/148‑1 (MQ=255)
                                           ccGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttc                                                                                                     <  1:2053207/148‑1 (MQ=255)
                                           ccGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttc                                                                                                     <  2:631461/148‑1 (MQ=255)
                                                aaGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCa                                                                                                >  1:2364342/1‑148 (MQ=255)
                                                aaGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCa                                                                                                >  1:3028682/1‑148 (MQ=255)
                                                 aGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAg                                                                                               >  2:2295640/1‑148 (MQ=255)
                                                  ggTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGc                                                                                              <  2:2186844/148‑1 (MQ=255)
                                                   gTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCg                                                                                             <  1:465995/148‑1 (MQ=255)
                                                   gTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCg                                                                                             <  2:1332533/148‑1 (MQ=255)
                                                    tGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttct                                                                                                    >  1:1838642/1‑140 (MQ=255)
                                                    tGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttct                                                                                                    <  2:1838642/140‑1 (MQ=255)
                                                    tGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGt                                                                                            >  1:96550/1‑148 (MQ=255)
                                                    tGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGt                                                                                            >  1:1025026/1‑148 (MQ=255)
                                                     gATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtg                                                                                           <  1:815359/148‑1 (MQ=255)
                                                     gATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtg                                                                                           >  2:2596687/1‑148 (MQ=255)
                                                     gATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtg                                                                                           <  1:2575183/148‑1 (MQ=255)
                                                      aTAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgc                                                                                          <  2:3424614/148‑1 (MQ=255)
                                                      aTAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgc                                                                                          <  1:3479268/148‑1 (MQ=255)
                                                       tAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgct                                                                                         >  1:200997/1‑148 (MQ=255)
                                                        aGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgctg                                                                                        <  1:501660/148‑1 (MQ=255)
                                                         gTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgctgc                                                                                       <  2:848397/148‑1 (MQ=255)
                                                         gTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgctgc                                                                                       <  1:207040/148‑1 (MQ=255)
                                                             aGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGt                                                                                   <  1:2313256/148‑1 (MQ=255)
                                                             aGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGt                                                                                   <  2:1553877/148‑1 (MQ=255)
                                                               gTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTaa                                                                                 >  1:1235435/1‑148 (MQ=255)
                                                                   aTCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGt                                                                             <  1:2199247/148‑1 (MQ=255)
                                                                    tCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTc                                                                            <  1:1496298/148‑1 (MQ=255)
                                                                    tCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTc                                                                            >  2:2328316/1‑148 (MQ=255)
                                                                     ccGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCa                                                                           >  2:2033811/1‑148 (MQ=255)
                                                                       gcgcgcTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATa                                                                         <  1:890317/148‑1 (MQ=255)
                                                                        cgcgcTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATaa                                                                        <  2:3345775/148‑1 (MQ=255)
                                                                            cTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTc                                                                    <  1:1703138/148‑1 (MQ=255)
                                                                            cTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTc                                                                    >  1:2252498/1‑148 (MQ=255)
                                                                            cTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTc                                                                    >  2:2245445/1‑148 (MQ=255)
                                                                                tgcttgctGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACTTCTTCa                                                                <  2:251536/148‑1 (MQ=255)
                                                                                tgcttgctGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCa                                                                <  1:1467401/148‑1 (MQ=255)
                                                                                tgcttgctGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCa                                                                <  1:1370417/148‑1 (MQ=255)
                                                                                       tgcGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGcaaca                                                         <  2:2960930/148‑1 (MQ=255)
                                                                                        gcGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGcaacaa                                                        <  2:1160176/148‑1 (MQ=255)
                                                                                         cGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAAt                                                       <  1:818631/148‑1 (MQ=255)
                                                                                         cGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAAt                                                       <  2:2842950/148‑1 (MQ=255)
                                                                                          ggAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATc                                                      <  2:2069482/148‑1 (MQ=255)
                                                                                          ggAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATc                                                      <  2:3196092/148‑1 (MQ=255)
                                                                                             gaCATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGa                                                   <  1:1722800/148‑1 (MQ=255)
                                                                                              aCATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAg                                                  >  2:1674695/1‑148 (MQ=255)
                                                                                               cATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAgg                                                 >  1:670794/1‑148 (MQ=255)
                                                                                               cATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAgg                                                 <  2:1795037/148‑1 (MQ=255)
                                                                                                aTGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTATTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGc                                                <  1:593485/148‑1 (MQ=255)
                                                                                                  gATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTg                                              <  2:1222046/148‑1 (MQ=255)
                                                                                                  gATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTg                                              >  1:2482921/1‑148 (MQ=255)
                                                                                                     cACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGACGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTgcgc                                           >  1:77092/1‑148 (MQ=255)
                                                                                                      aCTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCa                                          >  1:1822810/1‑148 (MQ=255)
                                                                                                      aCTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCa                                          >  1:3414831/1‑148 (MQ=255)
                                                                                                      aCTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCa                                          >  1:2636692/1‑148 (MQ=255)
                                                                                                       cTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAg                                         <  2:2364342/148‑1 (MQ=255)
                                                                                                       cTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAg                                         >  2:2469169/1‑148 (MQ=255)
                                                                                                        ttCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGa                                        <  1:1828900/148‑1 (MQ=255)
                                                                                                        ttCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGa                                        <  1:1829057/148‑1 (MQ=255)
                                                                                                         tCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGAt                                       >  2:2133156/1‑148 (MQ=255)
                                                                                                         tCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGAt                                       >  2:2133212/1‑148 (MQ=255)
                                                                                                            tCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCa                                    >  2:238403/1‑148 (MQ=255)
                                                                                                            tCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCa                                    >  2:2257191/1‑148 (MQ=255)
                                                                                                               gCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCNGCGTGCTGCCGGTAACCGTCATAANGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACa                                 <  1:2021138/148‑1 (MQ=255)
                                                                                                               gCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACa                                 <  2:3102376/148‑1 (MQ=255)
                                                                                                                cGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACAt                                >  2:2631420/1‑148 (MQ=255)
                                                                                                                cGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACAt                                >  2:1705993/1‑148 (MQ=255)
                                                                                                                cGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGACGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTACATGCAACAATCCGAGGCTGCGCAGATGCAACAt                                >  2:533113/1‑148 (MQ=255)
                                                                                                                  ttGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGa                              >  2:2712277/1‑148 (MQ=255)
                                                                                                                   tGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGAc                             <  1:1015928/148‑1 (MQ=255)
                                                                                                                   tGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCAGAGGCTGCGCAGATGCAACATGAc                             <  1:1543189/148‑1 (MQ=255)
                                                                                                                    gATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACt                            <  1:2148090/148‑1 (MQ=255)
                                                                                                                    gATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACt                            >  1:2995244/1‑148 (MQ=255)
                                                                                                                     aTTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACtt                           <  1:836381/148‑1 (MQ=255)
                                                                                                                      tttGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTc                          >  1:1546198/1‑148 (MQ=255)
                                                                                                                       ttGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTcc                         <  2:448315/148‑1 (MQ=255)
                                                                                                                           tCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGca                     >  2:1040319/1‑148 (MQ=255)
                                                                                                                           tCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGca                     >  1:2354224/1‑148 (MQ=255)
                                                                                                                            ccTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGcac                    >  1:3510313/1‑148 (MQ=255)
                                                                                                                            ccTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGcac                    <  2:2942914/148‑1 (MQ=255)
                                                                                                                               gATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCAcacc                 >  1:1061744/1‑148 (MQ=255)
                                                                                                                                aTCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCAcacca                >  1:3050045/1‑148 (MQ=255)
                                                                                                                                  cGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACaccacc              <  2:1822810/148‑1 (MQ=255)
                                                                                                                                     gCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGc           >  1:2608946/1‑148 (MQ=255)
                                                                                                                                        ggAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCAt        >  2:2777179/1‑148 (MQ=255)
                                                                                                                                            ttGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAg    >  1:3236795/1‑148 (MQ=255)
                                                                                                                                             tGCTAGAACCGCTGACGCCGTTCGGATTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAgg   <  1:2660227/148‑1 (MQ=255)
                                                                                                                                             tGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAgg   <  1:2660098/148‑1 (MQ=255)
                                                                                                                                              gCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGc  <  2:1055610/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGC  >  CP009273/4512548‑4512837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑