Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,584,255 A→C 100% L95L (CTT→CTG)  yjjL ← L‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,584,2550AC99.4% 806.9 / ‑5.3 203L95L (CTT→CTG) yjjLL‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base C (111/88);  total (112/91)
Fisher's exact test for biased strand distribution p-value = 3.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 203 total aligned reads displayed.

CAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAA  >  CP009273/4584113‑4584398
                                                                                                                                              |                                                                                                                                               
cAATCCCCATACCGATACGCACCCACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAAGAGTGGGCCGCAAGGCAGTTg                                                                                                                                            <  1:2174968/148‑1 (MQ=255)
    ccccATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCa                                                                                                                                        >  1:320566/1‑148 (MQ=255)
       cATACCGATACGCACCAACCCGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGCCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATc                                                                                                                                     <  2:1621638/148‑1 (MQ=255)
       cATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATc                                                                                                                                     >  1:1471732/1‑148 (MQ=255)
       cATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATc                                                                                                                                     <  2:1250263/148‑1 (MQ=255)
       cATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATc                                                                                                                                     <  2:1390016/148‑1 (MQ=255)
            cGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTa                                                                                                                                <  1:2423890/148‑1 (MQ=255)
             gATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTaa                                                                                                                               >  1:2566799/1‑148 (MQ=255)
               tACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGc                                                                                                                             >  2:2612525/1‑148 (MQ=255)
                aCGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCg                                                                                                                            >  1:2739711/1‑148 (MQ=255)
                 cGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGa                                                                                                                           >  2:732625/1‑148 (MQ=255)
                    aCCAACACGAACTGCGTAAAGTTGTGCACCATGCCGGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTg                                                                                                                        >  2:26975/1‑148 (MQ=255)
                     ccAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGa                                                                                                                       >  2:2763454/1‑148 (MQ=255)
                     ccAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGa                                                                                                                       >  2:1010299/1‑148 (MQ=255)
                      cAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaa                                                                                                                      <  1:1999155/148‑1 (MQ=255)
                      cAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaa                                                                                                                      >  2:1497496/1‑148 (MQ=255)
                       aaCACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaaa                                                                                                                     >  2:2529768/1‑148 (MQ=255)
                        acacGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGaaaa                                                                                                                    >  2:2091070/1‑148 (MQ=255)
                          acGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAaca                                                                                                                  <  1:2316379/148‑1 (MQ=255)
                           cGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAacac                                                                                                                 >  2:1067752/1‑148 (MQ=255)
                             aaCTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACgg                                                                                                               <  1:3111395/148‑1 (MQ=255)
                             aaCTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACgg                                                                                                               <  1:234188/148‑1 (MQ=255)
                               cTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGgag                                                                                                             <  1:87066/148‑1 (MQ=255)
                                  cGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGc                                                                                                          >  1:1578375/1‑148 (MQ=255)
                                    tAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCaa                                                                                                        >  1:1944996/1‑148 (MQ=255)
                                    tAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCaa                                                                                                        >  1:1946853/1‑148 (MQ=255)
                                             gCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGAt                                                                                               <  1:3434404/148‑1 (MQ=255)
                                                ccATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTc                                                                                            >  2:2349258/1‑148 (MQ=255)
                                                ccATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGAGTTc                                                                                            >  2:3070204/1‑148 (MQ=255)
                                                 cATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCg                                                                                           >  1:3174046/1‑148 (MQ=255)
                                                 cATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCg                                                                                           <  1:2686761/148‑1 (MQ=255)
                                                 cATGCCAGACAGTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCg                                                                                           <  2:2791204/148‑1 (MQ=255)
                                                  aTGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCgg                                                                                          <  1:1426581/148‑1 (MQ=255)
                                                   tGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGt                                                                                         <  2:2249214/148‑1 (MQ=255)
                                                        gACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCAc                                                                                    <  1:1120925/148‑1 (MQ=255)
                                                        gACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCAc                                                                                    >  2:3341737/1‑148 (MQ=255)
                                                        gACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCAc                                                                                    <  1:198893/148‑1 (MQ=255)
                                                        gACATTGCCTGGAACAGTGACCAGAAGAACAGCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCAc                                                                                    <  1:2017721/148‑1 (MQ=255)
                                                           aTTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTa                                                                                 <  1:366819/148‑1 (MQ=255)
                                                            ttGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGTATTTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCAGATTTCGGTGGCACTTaa                                                                                <  1:3500249/148‑1 (MQ=255)
                                                             tGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAAt                                                                               >  1:1216019/1‑148 (MQ=255)
                                                              gCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATc                                                                              <  2:856848/148‑1 (MQ=255)
                                                                cTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATccc                                                                            >  2:1123053/1‑148 (MQ=255)
                                                                 tGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCa                                                                           >  2:2009500/1‑148 (MQ=255)
                                                                  ggAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCaa                                                                          >  2:617713/1‑148 (MQ=255)
                                                                  ggAAAAGTGACCATTAGAACTTCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCTATAGTGTGCCGCATGGATGTTGCGCTTTCCCGTAAGCGAGTGAAAACACGGATAGAAAAGCGACGATTTCGGTGGCACTTTATCCCaa                                                                          <  2:880665/148‑1 (MQ=255)
                                                                    aaCAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAtt                                                                        >  1:3310008/1‑148 (MQ=255)
                                                                      cAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAttct                                                                      >  2:2453140/1‑148 (MQ=255)
                                                                      cAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAttct                                                                      >  2:2798846/1‑148 (MQ=255)
                                                                       aGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAttctt                                                                     <  1:2117456/148‑1 (MQ=255)
                                                                        gTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAAttcttc                                                                    <  1:1378766/148‑1 (MQ=255)
                                                                              agaagaACATCCCCAGTCCCAGGATCAGGCGTGGGCATTTACGATCCAATAGTGGGCCGCATGGCAGTTGCGCAATCCCGTAAGCGAGTGAATACACGTAGAGCAAAGCGCCGTTTTCGGTGGCACTTAATCCCAATTCTTCACGTAt                                                              <  2:1065535/148‑1 (MQ=255)
                                                                               gaagaaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATc                                                             >  2:3428434/1‑148 (MQ=255)
                                                                                 agaaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGt                                                           >  2:3413642/1‑148 (MQ=255)
                                                                                  gaaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGtt                                                          >  1:2650161/1‑148 (MQ=255)
                                                                                   aaCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTa                                                         <  2:1191622/148‑1 (MQ=255)
                                                                                    aCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTaa                                                        >  2:2555163/1‑148 (MQ=255)
                                                                                    aCATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTaa                                                        <  1:1065480/148‑1 (MQ=255)
                                                                                      aTCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACTATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAt                                                      <  1:2459330/148‑1 (MQ=255)
                                                                                      aTCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAt                                                      >  2:1576625/1‑148 (MQ=255)
                                                                                        ccccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAttt                                                    >  2:833996/1‑148 (MQ=255)
                                                                                        ccccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAttt                                                    >  1:1244384/1‑148 (MQ=255)
                                                                                        ccccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAAttt                                                    >  2:87088/1‑148 (MQ=255)
                                                                                         cccAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTg                                                   >  2:1720789/1‑148 (MQ=255)
                                                                                                 cAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGACTCGTTAAATTTGCTACCGAc                                           >  2:2996474/1‑148 (MQ=255)
                                                                                                 cAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGAc                                           >  1:208154/1‑148 (MQ=255)
                                                                                                 cAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGAc                                           >  1:715516/1‑148 (MQ=255)
                                                                                                  aGCATCCGGCGGGGGCCTTTACGATCCAATCGTGGGCCGCAAGGCAGTTGCGCAAGCCCGTAAGCGAGTGAAAACACGGCGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACa                                          <  1:2397746/148‑1 (MQ=255)
                                                                                                      tCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGa                                      <  2:2338680/148‑1 (MQ=255)
                                                                                                      tCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTAGCTACCGACAGCGa                                      >  1:2889067/1‑148 (MQ=255)
                                                                                                       ctggCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAACCCCGTAAGCGATTGAAAACACGGAGCGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACTAATCGTTAAATTTTCTACCGACAGCGaa                                     <  1:960177/146‑1 (MQ=255)
                                                                                                       cAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGaa                                     >  1:2551126/1‑148 (MQ=255)
                                                                                                         ggCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACt                                   >  2:500172/1‑148 (MQ=255)
                                                                                                          gCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTg                                  >  2:1411168/1‑148 (MQ=255)
                                                                                                          gCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTg                                  >  1:1802073/1‑148 (MQ=255)
                                                                                                           cGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGc                                 <  1:1565817/148‑1 (MQ=255)
                                                                                                           cGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGc                                 <  1:1680002/148‑1 (MQ=255)
                                                                                                            gTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCg                                <  2:3115169/148‑1 (MQ=255)
                                                                                                              gggCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGt                              >  2:1765073/1‑148 (MQ=255)
                                                                                                              gggCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGt                              >  1:1563627/1‑148 (MQ=255)
                                                                                                                gCCTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCg                            <  2:391707/148‑1 (MQ=255)
                                                                                                                  cTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACCCGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCgag                          <  1:2492803/148‑1 (MQ=255)
                                                                                                                  cTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCgag                          <  1:732625/148‑1 (MQ=255)
                                                                                                                  cTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCgag                          <  1:1956299/148‑1 (MQ=255)
                                                                                                                  cTTTACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCgag                          <  1:2917840/148‑1 (MQ=255)
                                                                                                                   tttACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCgaga                         <  2:1518218/148‑1 (MQ=255)
                                                                                                                   tttACGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCgaga                         <  2:245383/148‑1 (MQ=255)
                                                                                                                      aCGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATaa                      <  2:882844/148‑1 (MQ=255)
                                                                                                                      aCGATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATaa                      <  2:1448701/148‑1 (MQ=255)
                                                                                                                       cGATCCAATCGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAt                     <  1:2765229/148‑1 (MQ=255)
                                                                                                                        gATCCAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAAtt                    <  1:1700182/148‑1 (MQ=255)
                                                                                                                           ccAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAt                 >  1:1923568/1‑148 (MQ=255)
                                                                                                                            cAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAtt                >  2:2900268/1‑148 (MQ=255)
                                                                                                                             aaTAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATttatta               <  2:1961504/148‑1 (MQ=255)
                                                                                                                               tAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAcc             >  1:638108/1‑148 (MQ=255)
                                                                                                                               tAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAcc             >  1:1739464/1‑148 (MQ=255)
                                                                                                                                 gTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAccgc           >  1:1256257/1‑148 (MQ=255)
                                                                                                                                  tGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAccgcc          >  1:274926/1‑148 (MQ=255)
                                                                                                                                         cAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaaa   <  1:1615950/148‑1 (MQ=255)
                                                                                                                                         cAAGGCAGAGGCGCAATCCCGTAAGCGAGTGAAAACCCGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaaa   <  2:1318840/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCATGCCAGACATTGCCTGGAACAGTGACCAGAAGAACATCCCCAGTCCCAGCATCAGGCGTGGGCCTTTACGATCCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAA  >  CP009273/4584113‑4584398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑