Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,584,381 G→T 100% I53I (ATC→ATA)  yjjL ← L‑galactonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,584,3810GT99.0% 612.3 / ‑1.9 190I53I (ATC→ATA) yjjLL‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (87/101);  total (89/101)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 191 total aligned reads displayed.

CCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGATA  >  CP009273/4584236‑4584525
                                                                                                                                                 |                                                                                                                                                
ccAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAt                                                                                                                                                >  1:1923568/1‑148 (MQ=255)
ccAATAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACATAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTAt                                                                                                                                                >  1:2119851/1‑148 (MQ=255)
 cactcgTGGGCCGCAAGGCAGTTGCGCACTCCCGTCAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCCCGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCTAGATAATTTAtt                                                                                                                                               <  2:792257/143‑1 (MQ=255)
    tAGTGGGCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAcc                                                                                                                                            >  1:638108/1‑148 (MQ=255)
        gggCCGCAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTAccgccg                                                                                                                                        <  2:1591053/148‑1 (MQ=255)
              cAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaaa                                                                                                                                  <  1:2009500/148‑1 (MQ=255)
              cAAGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaaa                                                                                                                                  <  1:1615950/148‑1 (MQ=255)
              cAAGGCAGAGGCGCAATCCCGTAAGCGAGTGAAAACCCGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaaa                                                                                                                                  <  2:1318840/148‑1 (MQ=255)
               aaGGCAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCaaaaaa                                                                                                                                 >  2:279715/1‑148 (MQ=255)
                  gcaGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAaataa                                                                                                                              >  1:2160331/3‑148 (MQ=255)
                  gcaGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAaataa                                                                                                                              >  1:1909581/3‑148 (MQ=255)
                   caGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAaataat                                                                                                                             <  2:1577241/147‑1 (MQ=255)
                      ttGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATaac                                                                                                                          >  1:2865790/1‑148 (MQ=255)
                      ttGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATaac                                                                                                                          >  2:3079802/1‑148 (MQ=255)
                      ttGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATaac                                                                                                                          >  1:3130783/1‑148 (MQ=255)
                       tGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATaaca                                                                                                                         <  1:1733649/148‑1 (MQ=255)
                        tctcAATCCCGTAAGCGTGTGAAAACACGGAGAGCAAAGCGCCTATTTCGGTGGAACTTAATCCCATTTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATaacaa                                                                                                                        <  1:1244593/145‑1 (MQ=255)
                          gcAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAacaaca                                                                                                                      <  2:1256257/148‑1 (MQ=255)
                          gaaaTCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAacaaca                                                                                                                      <  2:1073426/146‑1 (MQ=255)
                           cAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACAt                                                                                                                     <  2:1800097/148‑1 (MQ=255)
                            aaTCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATg                                                                                                                    <  2:1964007/148‑1 (MQ=255)
                                  gTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCggtg                                                                                                              >  1:513307/1‑148 (MQ=255)
                                    aaGCGATTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCggtggt                                                                                                            <  1:3081100/148‑1 (MQ=255)
                                    aaGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCggtggt                                                                                                            <  1:1659024/148‑1 (MQ=255)
                                    aaGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCggtggt                                                                                                            >  1:3244085/1‑148 (MQ=255)
                                    aaGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCggtggt                                                                                                            >  1:2711164/1‑148 (MQ=255)
                                       cGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTg                                                                                                         >  1:2493792/1‑148 (MQ=255)
                                       cGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTg                                                                                                         >  1:2581265/1‑148 (MQ=255)
                                        gAGTTAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGa                                                                                                        <  1:1497496/148‑1 (MQ=255)
                                         aGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGaa                                                                                                       <  1:2792073/148‑1 (MQ=255)
                                         aGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGaa                                                                                                       <  2:2356002/148‑1 (MQ=255)
                                             aaaaCACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCg                                                                                                   >  2:2099619/1‑148 (MQ=255)
                                             aaaaCACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCg                                                                                                   <  2:3174046/148‑1 (MQ=255)
                                               aaCACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACACCATGGCGGTGGTTTGAATGCGtt                                                                                                 <  1:412658/148‑1 (MQ=255)
                                                acacGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGttt                                                                                                <  1:1185694/148‑1 (MQ=255)
                                                 cacGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTa                                                                                               >  2:3263878/1‑148 (MQ=255)
                                                  acGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGAGTTTaa                                                                                              >  2:3225163/1‑148 (MQ=255)
                                                   cGGAGATCAAATCGCAGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGATATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAAt                                                                                             <  2:320566/148‑1 (MQ=255)
                                                     gagaGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTc                                                                                           <  2:2566799/148‑1 (MQ=255)
                                                     gagaGCAAAGCGCCGATTTCGGTGGAACTTAATCCCTATTCTTCAGGAATCGTTAAATTTGCTACCTAAAGCGAACTGCGGTATTGATAATTTATTACCGACTCAAAAAATAATAACAACATGGAGGTGTTTTGAATGCGTTTAATTc                                                                                           <  2:2566755/148‑1 (MQ=255)
                                                        agCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCggg                                                                                        >  2:2416849/1‑148 (MQ=255)
                                                        agCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCggg                                                                                        >  1:661087/1‑148 (MQ=255)
                                                        agCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCggg                                                                                        >  2:574170/1‑148 (MQ=255)
                                                          cAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTa                                                                                      >  2:3413187/1‑148 (MQ=255)
                                                          cAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTa                                                                                      >  1:3189447/1‑148 (MQ=255)
                                                              gcgcCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTCCTGc                                                                                  >  2:2096221/1‑148 (MQ=255)
                                                               cgcCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCg                                                                                 <  1:1009063/148‑1 (MQ=255)
                                                               cgcCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCg                                                                                 >  2:666404/1‑148 (MQ=255)
                                                               cgcCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCg                                                                                 <  1:2064416/148‑1 (MQ=255)
                                                                   gATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGa                                                                             <  2:1754136/148‑1 (MQ=255)
                                                                   gATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGa                                                                             <  2:1216019/148‑1 (MQ=255)
                                                                   gATTTCGGTGGCACTTAATCCCAAGTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGa                                                                             <  2:363498/148‑1 (MQ=255)
                                                                    aTTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGaa                                                                            <  1:362051/148‑1 (MQ=255)
                                                                    aTTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTGTAATTCGGGTACTGCGTTGaa                                                                            >  2:3473474/1‑148 (MQ=255)
                                                                    aTTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTGTGAATGCGTTTAATTCGGGTACTGCGTTGaa                                                                            >  2:3473468/1‑148 (MQ=255)
                                                                      ttCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACt                                                                          >  1:733524/1‑148 (MQ=255)
                                                                       tCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTa                                                                         >  1:3340801/1‑148 (MQ=255)
                                                                       tCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTa                                                                         <  1:2611501/148‑1 (MQ=255)
                                                                        cGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTaa                                                                        <  1:1411185/148‑1 (MQ=255)
                                                                             gCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCAt                                                                   >  1:2550022/1‑148 (MQ=255)
                                                                             gCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCAt                                                                   <  2:1563627/148‑1 (MQ=255)
                                                                              cACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATc                                                                  <  1:2529768/148‑1 (MQ=255)
                                                                                 ttAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCtgg                                                               <  1:91054/148‑1 (MQ=255)
                                                                                 ttAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCtgg                                                               <  1:91035/148‑1 (MQ=255)
                                                                                  tAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCtggt                                                              >  2:3513295/1‑148 (MQ=255)
                                                                                    aTCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCtggtgg                                                            <  1:26975/148‑1 (MQ=255)
                                                                                      cccAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCa                                                          <  2:3310008/148‑1 (MQ=255)
                                                                                      cccAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCa                                                          >  1:1559499/1‑148 (MQ=255)
                                                                                            tcttcACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGaa                                                    <  1:3082332/148‑1 (MQ=255)
                                                                                              ttcACGAATCGTTAAATTTTCTACCGACAGCGAACTGCGGTCTAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAata                                                  <  1:3376724/148‑1 (MQ=255)
                                                                                               tcACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAatat                                                 <  1:682297/148‑1 (MQ=255)
                                                                                               tcACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAatat                                                 <  1:1067752/148‑1 (MQ=255)
                                                                                                    aaTCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGcc                                            >  1:3480692/1‑148 (MQ=255)
                                                                                                    aaTCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGcc                                            >  1:1743460/1‑148 (MQ=255)
                                                                                                           aaaTTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAg                                     <  1:1010299/148‑1 (MQ=255)
                                                                                                           aaaTTTGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGAGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAg                                     <  1:2763454/148‑1 (MQ=255)
                                                                                                               ttGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACACCATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATgg                                 <  2:2669385/148‑1 (MQ=255)
                                                                                                               ttGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATgg                                 <  2:1945774/148‑1 (MQ=255)
                                                                                                                tGCTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGa                                >  1:532599/1‑148 (MQ=255)
                                                                                                                  cTACCGACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAACAAATAATAACAACATGGCGGTGGTTTGATTGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGt                              <  2:2889067/148‑1 (MQ=255)
                                                                                                                  cTACCGACAGCGAACTGCGGTCGAGATAATATATTACCGCCGCAAAAAATAATAAAAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCTTAGAACTAATCCATCTTCTGGAACGTGAATATCTGCCGAAGAAGATCGAGt                              >  2:2774096/1‑148 (MQ=255)
                                                                                                                       gACAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATg                         <  2:402825/148‑1 (MQ=255)
                                                                                                                         cAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATgac                       >  1:2798917/1‑147 (MQ=255)
                                                                                                                         cAGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATgaa                       >  1:1303069/1‑148 (MQ=255)
                                                                                                                          aGCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATgaag                      <  2:1923568/148‑1 (MQ=255)
                                                                                                                           gCGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATgaaga                     <  2:2550022/148‑1 (MQ=255)
                                                                                                                            cGAACTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATgaagaa                    <  1:3262419/148‑1 (MQ=255)
                                                                                                                              aaCTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAAcg                  <  1:617713/148‑1 (MQ=255)
                                                                                                                               aCTGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAAcgc                 >  1:2492566/1‑148 (MQ=255)
                                                                                                                                 tGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCgg               >  1:2884852/1‑148 (MQ=255)
                                                                                                                                 tGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCgg               >  1:1135102/1‑148 (MQ=255)
                                                                                                                                 tGCGGTCGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGGGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCgg               >  1:2797187/1‑148 (MQ=255)
                                                                                                                                       cGAGATAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGAt         >  2:190473/1‑148 (MQ=255)
                                                                                                                                        gagaTAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATg        <  2:2739711/148‑1 (MQ=255)
                                                                                                                                          gaTAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGt      <  1:60956/148‑1 (MQ=255)
                                                                                                                                          gaTAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGt      <  1:1765073/148‑1 (MQ=255)
                                                                                                                                          gaTAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGt      <  1:1398743/148‑1 (MQ=255)
                                                                                                                                            tAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGa    >  2:1749171/1‑148 (MQ=255)
                                                                                                                                            tAATTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGa    >  2:766982/1‑148 (MQ=255)
                                                                                                                                              aTTTATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGata  <  2:1471732/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCAATAGTGGGCCGCAAGGAAGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGCAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATGGTGATA  >  CP009273/4584236‑4584525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑