Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 32,802 C→A 100% I662I (ATC→ATA)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927332,8020CA98.8% 574.0 / ‑3.8 200I662I (ATC→ATA) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (1/6);  new base A (93/100);  total (94/106)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 200 total aligned reads displayed.

ACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGG  >  CP009273/32657‑32942
                                                                                                                                                 |                                                                                                                                            
acagCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAgg                                                                                                                                            <  2:1269317/145‑1 (MQ=255)
acagCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAgg                                                                                                                                            >  2:148922/4‑148 (MQ=255)
acagCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAgg                                                                                                                                            <  2:3017382/145‑1 (MQ=255)
acagCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAgg                                                                                                                                            >  2:3187072/4‑148 (MQ=255)
    ccTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCAcc                                                                                                                                        >  1:1348122/1‑148 (MQ=255)
      tctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAg                                                                                                                                      >  1:2822851/1‑148 (MQ=255)
       ctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGc                                                                                                                                     <  2:2517010/148‑1 (MQ=255)
           ttCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCgg                                                                                                                                 >  1:3077207/1‑148 (MQ=255)
              gAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATg                                                                                                                              <  1:2446078/148‑1 (MQ=255)
               aGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGc                                                                                                                             <  2:2150177/148‑1 (MQ=255)
               aGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGc                                                                                                                             >  1:1647955/1‑148 (MQ=255)
                gCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCt                                                                                                                            >  2:2310701/1‑148 (MQ=255)
                gCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCt                                                                                                                            <  2:3130099/148‑1 (MQ=255)
                gCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCt                                                                                                                            >  2:2847442/1‑148 (MQ=255)
                gCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCt                                                                                                                            >  1:1321732/1‑148 (MQ=255)
                  cGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTAt                                                                                                                          <  1:2673967/148‑1 (MQ=255)
                   ggTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATc                                                                                                                         >  2:2110183/1‑148 (MQ=255)
                    gTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCg                                                                                                                        >  2:1773897/1‑148 (MQ=255)
                      aaCTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGAc                                                                                                                      >  2:2894464/1‑148 (MQ=255)
                       aCTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGAcc                                                                                                                     <  2:2628608/148‑1 (MQ=255)
                         tctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGt                                                                                                                   >  1:2643864/1‑148 (MQ=255)
                             gAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCag                                                                                                               >  2:627500/1‑148 (MQ=255)
                              aaGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCaga                                                                                                              >  1:1923920/1‑148 (MQ=255)
                                gATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCagaag                                                                                                            <  2:2247705/148‑1 (MQ=255)
                                       tGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGa                                                                                                     <  1:2961042/148‑1 (MQ=255)
                                        ggAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGaa                                                                                                    <  1:2625188/148‑1 (MQ=255)
                                        ggAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGaa                                                                                                    <  1:1515843/148‑1 (MQ=255)
                                         gAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAAc                                                                                                   >  1:1316980/1‑148 (MQ=255)
                                          aaaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACg                                                                                                  <  2:1344673/148‑1 (MQ=255)
                                          aaaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACg                                                                                                  >  1:80541/1‑148 (MQ=255)
                                          aaaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACg                                                                                                  <  2:3304493/148‑1 (MQ=255)
                                           aaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGc                                                                                                 >  2:3427751/1‑148 (MQ=255)
                                             tCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCtt                                                                                               <  2:3251718/148‑1 (MQ=255)
                                             tCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCtt                                                                                               <  2:3475787/148‑1 (MQ=255)
                                              cGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTc                                                                                              >  1:2034753/1‑148 (MQ=255)
                                                tGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGACCGCTTCCa                                                                                            >  2:2394140/1‑148 (MQ=255)
                                                    tATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCAt                                                                                        >  1:1462824/1‑148 (MQ=255)
                                                       cGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCg                                                                                     <  1:2730340/148‑1 (MQ=255)
                                                        gagaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCgg                                                                                    <  1:2802382/148‑1 (MQ=255)
                                                          gaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGtt                                                                                  <  1:3478652/148‑1 (MQ=255)
                                                          gaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGAATGCGGtt                                                                                  >  2:3146713/1‑148 (MQ=255)
                                                           aaGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCTTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTg                                                                                 <  2:162086/148‑1 (MQ=255)
                                                           aaGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTg                                                                                 <  2:1592792/148‑1 (MQ=255)
                                                             gCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAg                                                                               >  2:2370036/1‑148 (MQ=255)
                                                                     gCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaa                                                                       >  2:2769687/1‑148 (MQ=255)
                                                                     gCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaa                                                                       <  2:144415/148‑1 (MQ=255)
                                                                      cGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaa                                                                      >  2:4847/1‑148 (MQ=255)
                                                                         tATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactg                                                                   >  1:126969/1‑148 (MQ=255)
                                                                          aTCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactga                                                                  >  2:943003/1‑148 (MQ=255)
                                                                           tCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactgaa                                                                 >  2:660986/1‑148 (MQ=255)
                                                                           tCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactgaa                                                                 >  2:2732466/1‑148 (MQ=255)
                                                                           tCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactgaa                                                                 >  2:1502425/1‑148 (MQ=255)
                                                                              tCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAaaca                                                              <  1:1565196/148‑1 (MQ=255)
                                                                              tCCAGTACGGCGGTCAGACCCAGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTAGCGGTTATAGGAACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAaaca                                                              <  1:3509293/148‑1 (MQ=255)
                                                                               ccAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTACAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAaacaa                                                             <  1:453098/148‑1 (MQ=255)
                                                                                       gcggTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGaa                                                     <  2:1233760/148‑1 (MQ=255)
                                                                                        cggTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAAc                                                    >  1:58900/1‑148 (MQ=255)
                                                                                          gTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGc                                                  <  1:2406360/148‑1 (MQ=255)
                                                                                          gTCAGACCCCGCTGAAACGGGCGCGCGCGCTGGAAGCTGCTGGCGTACGGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGc                                                  <  1:2406859/148‑1 (MQ=255)
                                                                                           tCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGcc                                                 >  2:2072088/1‑148 (MQ=255)
                                                                                            cAGACCCCGCTTAAACTGGCGCGCGCGCTGGAAGCTGCGGGCGTACGGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCa                                                <  1:219651/148‑1 (MQ=255)
                                                                                            cAGACCCCGCTGAAACTGGCGCGCGCGCTGGACGCTGCTGGCGTACCGTTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCa                                                <  1:2806983/148‑1 (MQ=255)
                                                                                            cAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCa                                                <  2:2962639/148‑1 (MQ=255)
                                                                                             aGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGTTTATAGGCACCAGCCCGTTTGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCTTGGGGTTGAGCGTCTTAAACTGAAACAACCGGCGAACGCCAc                                               <  1:3334235/148‑1 (MQ=255)
                                                                                               aCCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGACACAACCGGCGAACGCCACCg                                             >  1:3016324/1‑148 (MQ=255)
                                                                                               aCCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCg                                             >  1:1102554/1‑148 (MQ=255)
                                                                                                 cccGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGtt                                           <  2:2449394/148‑1 (MQ=255)
                                                                                                 cccGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGtt                                           <  2:1837565/148‑1 (MQ=255)
                                                                                                       gAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCt                                     >  1:419537/1‑148 (MQ=255)
                                                                                                       gAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCt                                     >  1:2589861/1‑148 (MQ=255)
                                                                                                         aaCTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTAt                                   <  2:696365/148‑1 (MQ=255)
                                                                                                         aaCTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTAt                                   <  2:2136435/148‑1 (MQ=255)
                                                                                                               cgcgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAAt                             <  2:3282001/148‑1 (MQ=255)
                                                                                                                   cgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCg                         <  1:2342587/148‑1 (MQ=255)
                                                                                                                   cgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCg                         <  1:2342920/148‑1 (MQ=255)
                                                                                                                    gcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCgg                        <  2:2822767/148‑1 (MQ=255)
                                                                                                                     cgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGt                       >  2:3359227/1‑148 (MQ=255)
                                                                                                                       cTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTag                     <  1:427435/148‑1 (MQ=255)
                                                                                                                        tGGCAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTaga                    <  2:435226/148‑1 (MQ=255)
                                                                                                                        tGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTaga                    <  2:76250/148‑1 (MQ=255)
                                                                                                                          gAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAgaga                  >  1:511797/1‑148 (MQ=255)
                                                                                                                           acgctgctGGCGTACCGGTTATCGGCACCAGCCGGGTTGCTATCGACCGTGAAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGaa                 <  1:2364959/146‑1 (MQ=255)
                                                                                                                           aaGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGaa                 <  1:1747419/148‑1 (MQ=255)
                                                                                                                            aGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAg                <  1:3177374/148‑1 (MQ=255)
                                                                                                                             gctgctGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAgg               >  2:3124570/1‑148 (MQ=255)
                                                                                                                             gctgctGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAgg               >  1:1543424/1‑148 (MQ=255)
                                                                                                                              ctgctgGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGc              >  2:1005903/1‑148 (MQ=255)
                                                                                                                               tgctgGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCg             <  1:1773897/148‑1 (MQ=255)
                                                                                                                                gctgGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGa            >  2:25419/1‑148 (MQ=255)
                                                                                                                                 ctgGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGaa           <  2:2034753/148‑1 (MQ=255)
                                                                                                                                  tgGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGaaa          <  2:3079008/148‑1 (MQ=255)
                                                                                                                                   ggCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAag         >  2:287911/1‑148 (MQ=255)
                                                                                                                                   ggCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAag         <  1:2927928/148‑1 (MQ=255)
                                                                                                                                      gTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAgaga      <  1:148922/148‑1 (MQ=255)
                                                                                                                                       tACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGATAAGGCGAAAGAGAt     <  2:1152565/148‑1 (MQ=255)
                                                                                                                                       tACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGAt     <  1:3484175/148‑1 (MQ=255)
                                                                                                                                       tACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGAt     <  1:3296629/148‑1 (MQ=255)
                                                                                                                                       tACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGAt     <  2:2643864/148‑1 (MQ=255)
                                                                                                                                          cGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTgg  <  1:1357708/148‑1 (MQ=255)
                                                                                                                                          cGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTgg  >  1:935770/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
ACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGG  >  CP009273/32657‑32942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑