Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,588,180 G→T 100% S360* (TCA→TAA)  opgB ← phosphoglycerol transferases I and II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,588,1800GT98.9% 604.8 / ‑1.8 190S360* (TCA→TAA) opgBphosphoglycerol transferases I and II
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (87/100);  total (88/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 190 total aligned reads displayed.

TTGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCACG  >  CP009273/4588039‑4588324
                                                                                                                                             |                                                                                                                                                
 tGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGAtt                                                                                                                                           <  1:192766/148‑1 (MQ=255)
    ggTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCgg                                                                                                                                        <  1:1122659/148‑1 (MQ=255)
    ggTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCgg                                                                                                                                        <  1:1036566/148‑1 (MQ=255)
      tATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGtt                                                                                                                                      >  1:2775157/1‑148 (MQ=255)
       aTTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGttt                                                                                                                                     <  1:3279388/148‑1 (MQ=255)
           ccACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCg                                                                                                                                 >  2:545536/1‑148 (MQ=255)
              cGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCa                                                                                                                              >  1:1532485/1‑148 (MQ=255)
              cGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCa                                                                                                                              >  2:1037752/1‑148 (MQ=255)
                  gtgtTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAAt                                                                                                                          >  1:2197853/1‑148 (MQ=255)
                  gtgtTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAAt                                                                                                                          <  1:574342/148‑1 (MQ=255)
                   tgttgtTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATc                                                                                                                         <  2:1908011/148‑1 (MQ=255)
                   tgttgtTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATc                                                                                                                         <  2:135428/148‑1 (MQ=255)
                    gttgttCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCa                                                                                                                        >  1:2576159/1‑148 (MQ=255)
                      tgttCATAGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCata                                                                                                                      <  1:798099/148‑1 (MQ=255)
                         tCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATAttt                                                                                                                   >  1:1839886/1‑148 (MQ=255)
                         tCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATAttt                                                                                                                   >  2:222755/1‑148 (MQ=255)
                           aTCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATAttttt                                                                                                                 <  1:45155/148‑1 (MQ=255)
                               cTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCg                                                                                                             >  1:190672/1‑148 (MQ=255)
                                 aaaTGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGt                                                                                                           >  2:1248597/1‑148 (MQ=255)
                                 aaaTGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGt                                                                                                           >  2:2680517/1‑148 (MQ=255)
                                  aaTGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGtt                                                                                                          >  1:653455/1‑148 (MQ=255)
                                   aTGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTa                                                                                                         >  2:554098/1‑148 (MQ=255)
                                   aTGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTa                                                                                                         <  1:1175951/148‑1 (MQ=255)
                                     ggTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACa                                                                                                       <  2:2621634/148‑1 (MQ=255)
                                     ggTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACa                                                                                                       <  1:3275485/148‑1 (MQ=255)
                                       tCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAgg                                                                                                     <  2:934527/148‑1 (MQ=255)
                                          gaagaGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTAc                                                                                                  >  1:73265/1‑148 (MQ=255)
                                             gagaCGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACgag                                                                                               <  2:2167069/148‑1 (MQ=255)
                                             gagaCGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACgag                                                                                               <  2:2169328/148‑1 (MQ=255)
                                               gacgacGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACgagag                                                                                             <  1:2326465/148‑1 (MQ=255)
                                                  gacgaTGACGGTATCTTTACACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATa                                                                                          <  2:2078674/148‑1 (MQ=255)
                                                   acgaTGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATaa                                                                                         >  2:2436179/1‑148 (MQ=255)
                                                   acgaTGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATaa                                                                                         >  2:2294533/1‑148 (MQ=255)
                                                      aTGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAAc                                                                                      >  2:2378706/1‑148 (MQ=255)
                                                        gACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCa                                                                                    >  2:3116870/1‑148 (MQ=255)
                                                         aCGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCAt                                                                                   >  1:2622575/1‑148 (MQ=255)
                                                          cGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATc                                                                                  <  2:2463466/148‑1 (MQ=255)
                                                          cGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATc                                                                                  <  1:1474033/148‑1 (MQ=255)
                                                            gTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCg                                                                                >  1:2984657/1‑148 (MQ=255)
                                                             tATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCgg                                                                               >  1:2402636/1‑148 (MQ=255)
                                                             tATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCgg                                                                               >  1:2402440/1‑148 (MQ=255)
                                                              aTCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCggg                                                                              <  1:3458312/148‑1 (MQ=255)
                                                              aTCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCggg                                                                              >  2:205108/1‑148 (MQ=255)
                                                                cTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTg                                                                            <  2:49007/148‑1 (MQ=255)
                                                                  ttAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGAt                                                                          >  2:3196740/1‑148 (MQ=255)
                                                                      aCCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGt                                                                      <  2:2226304/148‑1 (MQ=255)
                                                                      aCCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGt                                                                      <  2:3327240/148‑1 (MQ=255)
                                                                       ccACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTa                                                                     <  1:1190312/148‑1 (MQ=255)
                                                                         aCGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATc                                                                   >  2:2093436/1‑148 (MQ=255)
                                                                         aCGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATc                                                                   >  2:2866524/1‑148 (MQ=255)
                                                                          cGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCg                                                                  >  2:1158888/1‑148 (MQ=255)
                                                                          cGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCg                                                                  >  2:1747672/1‑148 (MQ=255)
                                                                           ggTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGa                                                                 <  2:2876112/148‑1 (MQ=255)
                                                                           ggTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGa                                                                 <  2:1909286/148‑1 (MQ=255)
                                                                             tGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACt                                                               <  2:3242645/148‑1 (MQ=255)
                                                                             tGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTAATGATTAGGTTTAACGTCAAAATCAAATTTTTTTCGGTTAAAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGaat                                                               >  1:3247635/1‑146 (MQ=255)
                                                                                cGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTc                                                            <  1:1770065/148‑1 (MQ=255)
                                                                                   tttGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCGACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAgg                                                         <  2:1109542/148‑1 (MQ=255)
                                                                                   tttGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAgg                                                         <  2:3418112/148‑1 (MQ=255)
                                                                                       aTTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTaa                                                     >  1:1840219/1‑148 (MQ=255)
                                                                                       aTTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTaa                                                     <  1:2269950/148‑1 (MQ=255)
                                                                                        ttttGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTaaa                                                    <  2:3459382/148‑1 (MQ=255)
                                                                                           tgttgATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAg                                                 <  1:1309152/148‑1 (MQ=255)
                                                                                           tgttgATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAg                                                 <  1:3055379/148‑1 (MQ=255)
                                                                                              tgATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTga                                              >  2:1099020/1‑148 (MQ=255)
                                                                                              tgATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTga                                              >  2:3288583/1‑148 (MQ=255)
                                                                                                aTAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTgaga                                            >  2:986064/1‑148 (MQ=255)
                                                                                                  aaaCGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAAt                                          >  1:3310713/1‑148 (MQ=255)
                                                                                                  aaaCGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAAt                                          <  2:1281374/148‑1 (MQ=255)
                                                                                                    aCGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCg                                        <  1:408180/148‑1 (MQ=255)
                                                                                                        cgcgATGTTCTCCTGGCTGCAACTTACCGAGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGAGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGa                                    <  2:6929/148‑1 (MQ=255)
                                                                                                         gcgATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGAc                                   <  2:1999851/148‑1 (MQ=255)
                                                                                                         gcgATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGAc                                   <  2:351206/148‑1 (MQ=255)
                                                                                                         gcgATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGAc                                   <  2:2451663/148‑1 (MQ=255)
                                                                                                          cgATGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAACACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGAcc                                  <  2:2140364/148‑1 (MQ=255)
                                                                                                            aTGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTg                                >  1:2449067/1‑148 (MQ=255)
                                                                                                             tGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGa                               >  1:2101109/1‑148 (MQ=255)
                                                                                                             tGTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGa                               >  2:1533355/1‑148 (MQ=255)
                                                                                                              gTTCTCCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAg                              >  2:1226437/1‑148 (MQ=255)
                                                                                                                tctcCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCg                            <  1:1622930/148‑1 (MQ=255)
                                                                                                                tctcCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCg                            <  2:895285/148‑1 (MQ=255)
                                                                                                                 ctcCTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCgg                           <  1:828727/148‑1 (MQ=255)
                                                                                                                   ccTGGCTGCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGa                         >  1:3158878/1‑148 (MQ=255)
                                                                                                                          gCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGctct                  <  2:1296864/148‑1 (MQ=255)
                                                                                                                          gCAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGcgct                  >  2:2077103/1‑148 (MQ=255)
                                                                                                                           cAACTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCtt                 <  1:1378832/148‑1 (MQ=255)
                                                                                                                              cTTACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCaa              <  2:2197900/148‑1 (MQ=255)
                                                                                                                               ttACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCaaa             >  2:1396211/1‑148 (MQ=255)
                                                                                                                               ttACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCaaa             <  1:1610048/148‑1 (MQ=255)
                                                                                                                               ttACCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCaaa             >  2:1484122/1‑148 (MQ=255)
                                                                                                                                tACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAAc            <  2:1661128/148‑1 (MQ=255)
                                                                                                                                 aCCGCGCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACt           >  2:2493158/1‑148 (MQ=255)
                                                                                                                                   cgcgCTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACttt         <  2:653391/148‑1 (MQ=255)
                                                                                                                                     cgcTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACttttt       >  1:2943462/1‑148 (MQ=255)
                                                                                                                                     cgcTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACttttt       <  2:579454/148‑1 (MQ=255)
                                                                                                                                       cTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTcc     <  1:802707/148‑1 (MQ=255)
                                                                                                                                       cTGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTcc     >  1:941345/1‑148 (MQ=255)
                                                                                                                                        tGAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCa    <  2:687306/148‑1 (MQ=255)
                                                                                                                                         gAATTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCAc   >  1:1437337/1‑148 (MQ=255)
                                                                                                                                          aaTTACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCACg  >  2:2222593/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                
TTGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTTTTTGCGGTTACAGGTACGAGAGATAAAACCATCCGGGTGATGGGTATCGACTGTCAGGGTAAACAGTGAGAATCGCTGACCTGAGCGGGAAAGCTCTTCAAACTTTTTCCACG  >  CP009273/4588039‑4588324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑