Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,591,139 C→A 100% D66Y (GAT→TAT)  dnaT ← DNA biosynthesis protein (primosomal protein I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,591,1390CA99.0% 608.9 / ‑1.6 193D66Y (GAT→TAT) dnaTDNA biosynthesis protein (primosomal protein I)
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (101/89);  total (103/90)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 193 total aligned reads displayed.

TCTCTTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTG  >  CP009273/4590994‑4591283
                                                                                                                                                 |                                                                                                                                                
tctctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGCGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGc                                                                                                                                                >  2:132952/1‑148 (MQ=255)
tctctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGc                                                                                                                                                <  1:203944/148‑1 (MQ=255)
tctctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGc                                                                                                                                                >  2:1959452/1‑148 (MQ=255)
tctctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGc                                                                                                                                                >  1:1470289/1‑148 (MQ=255)
 ctctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCt                                                                                                                                               >  2:1737142/1‑148 (MQ=255)
   ctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTc                                                                                                                                             <  2:2853281/148‑1 (MQ=255)
    ttAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTcc                                                                                                                                            <  1:2506022/148‑1 (MQ=255)
           cACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGAcgc                                                                                                                                     >  2:2048481/1‑148 (MQ=255)
           cACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGAcgc                                                                                                                                     >  2:45061/1‑148 (MQ=255)
                    tAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCttt                                                                                                                            >  2:3142442/1‑148 (MQ=255)
                    tAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCttt                                                                                                                            >  1:688273/1‑148 (MQ=255)
                     aGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCtttt                                                                                                                           <  1:3271482/148‑1 (MQ=255)
                     aGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCtttt                                                                                                                           <  1:3271509/148‑1 (MQ=255)
                        gccgccAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTttctt                                                                                                                        <  2:1508656/148‑1 (MQ=255)
                          cgccAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTcc                                                                                                                      <  2:1566130/148‑1 (MQ=255)
                            ccAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAg                                                                                                                    <  1:2835148/148‑1 (MQ=255)
                              aGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAgcg                                                                                                                  <  2:2673484/148‑1 (MQ=255)
                               ggCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAgcgc                                                                                                                 >  2:1161822/1‑148 (MQ=255)
                                gCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGcc                                                                                                                >  1:3112437/1‑148 (MQ=255)
                                   gATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCcagc                                                                                                             <  1:2087879/148‑1 (MQ=255)
                                   gATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCcagc                                                                                                             >  2:3273053/1‑148 (MQ=255)
                                     tAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCcagcag                                                                                                           >  1:3403511/1‑148 (MQ=255)
                                         aTCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCACATTTCTACAGCAACGCGTCGGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCa                                                                                                       >  1:3448134/1‑148 (MQ=255)
                                          tCGGCATCGGGTTCCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCCGCAGTTCAg                                                                                                      >  2:1394970/1‑148 (MQ=255)
                                           cGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGc                                                                                                     <  2:352595/148‑1 (MQ=255)
                                           cGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGc                                                                                                     >  1:94267/1‑148 (MQ=255)
                                           cGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGc                                                                                                     <  1:2095901/148‑1 (MQ=255)
                                            ggCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGcc                                                                                                    <  1:3499574/148‑1 (MQ=255)
                                            ggCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGcc                                                                                                    <  1:3499305/148‑1 (MQ=255)
                                              cATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcag                                                                                                  >  2:2369499/1‑148 (MQ=255)
                                                 cGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcg                                                                                               <  2:302337/148‑1 (MQ=255)
                                                   ggTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtg                                                                                             >  1:2886258/1‑148 (MQ=255)
                                                   ggTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtg                                                                                             <  1:2718479/148‑1 (MQ=255)
                                                    gTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtgc                                                                                            <  2:1328362/148‑1 (MQ=255)
                                                         cAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtgcaggcg                                                                                       <  2:2537139/148‑1 (MQ=255)
                                                         cAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtgcaggcg                                                                                       >  1:1133496/1‑148 (MQ=255)
                                                           gTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtgcaggcgtg                                                                                     <  1:1761883/148‑1 (MQ=255)
                                                               ggAGACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACgg                                                                                 <  1:1295031/148‑1 (MQ=255)
                                                                 aTACATGGCGAATTTCCCCCTGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCa                                                                               <  1:3065215/148‑1 (MQ=255)
                                                                 aTACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCa                                                                               <  1:3064987/148‑1 (MQ=255)
                                                                 aTACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCa                                                                               >  1:519294/1‑148 (MQ=255)
                                                                 aTACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGATTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCa                                                                               >  2:2642978/1‑148 (MQ=255)
                                                                 aTACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGATTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCa                                                                               >  2:524887/1‑148 (MQ=255)
                                                                  tACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCAt                                                                              <  1:1008191/148‑1 (MQ=255)
                                                                     aTGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATaaa                                                                           >  2:1982705/1‑148 (MQ=255)
                                                                       ggCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAAc                                                                         >  2:479603/1‑148 (MQ=255)
                                                                       ggCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAAc                                                                         >  2:1826414/1‑148 (MQ=255)
                                                                        gCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACg                                                                        >  1:1272112/1‑148 (MQ=255)
                                                                        gCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACg                                                                        >  1:3021338/1‑148 (MQ=255)
                                                                             tttCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCggg                                                                   >  2:427892/1‑148 (MQ=255)
                                                                               tCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAACACGCCGGGGc                                                                 <  1:2615939/148‑1 (MQ=255)
                                                                                ccccATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCa                                                                <  2:94267/148‑1 (MQ=255)
                                                                                    aTGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGt                                                            <  2:1250147/148‑1 (MQ=255)
                                                                                     tGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGtt                                                           >  1:320455/1‑148 (MQ=255)
                                                                                          aCAGCAAGGGGAGTGGGTTGGGTTTCGTGATAGAGTTGATCGGCCTGAGCGACTTAGCTGCCCGGACGGGCGAGCTTTTCTTCATGCGCGTGAAGTTCAGCCAGGCGTGCAGGCTTGTCGGCATAAAACGCCGGGGCATTGTTAGcta                                                      <  2:378057/148‑3 (MQ=255)
                                                                                          aCAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCTACATAGCTTCCCGGACGCGCCAGCTTTTCTTCAAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCaa                                                      <  1:117321/148‑1 (MQ=255)
                                                                                          aCAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCaa                                                      <  2:3319826/148‑1 (MQ=255)
                                                                                          aCAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCaa                                                      <  2:2786715/148‑1 (MQ=255)
                                                                                           cAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCaaa                                                     >  1:1650487/1‑148 (MQ=255)
                                                                                            aGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAAt                                                    <  2:2138792/148‑1 (MQ=255)
                                                                                            aGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAAt                                                    <  1:132862/148‑1 (MQ=255)
                                                                                            aGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAAt                                                    <  2:369765/148‑1 (MQ=255)
                                                                                              cAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATAc                                                  >  1:3353544/1‑148 (MQ=255)
                                                                                                aCGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACgg                                                <  2:320455/148‑1 (MQ=255)
                                                                                                aCGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACgg                                                >  2:933334/1‑148 (MQ=255)
                                                                                                 cGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGc                                               >  2:477152/1‑148 (MQ=255)
                                                                                                  gggAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCa                                              <  2:1524581/148‑1 (MQ=255)
                                                                                                    gAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAAc                                            >  2:343559/1‑148 (MQ=255)
                                                                                                    gAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAAc                                            >  2:2113420/1‑148 (MQ=255)
                                                                                                        ggCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACcaca                                        <  1:1014974/148‑1 (MQ=255)
                                                                                                           ttGCGGTTCCTGATAGAGTTGATCGTCAAGAGCGACATTGCTTCCGGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCTGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCg                                     <  2:724613/148‑1 (MQ=255)
                                                                                                             gCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGcc                                   <  2:2436406/148‑1 (MQ=255)
                                                                                                              cGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCt                                  <  2:2963426/148‑1 (MQ=255)
                                                                                                                gTTCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTc                                >  2:2568036/1‑148 (MQ=255)
                                                                                                                 ttCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCa                               >  2:1439231/1‑148 (MQ=255)
                                                                                                                  tCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCCGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAg                              <  2:73804/148‑1 (MQ=255)
                                                                                                                  tCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAg                              <  2:419478/148‑1 (MQ=255)
                                                                                                                  tCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAg                              <  2:2930642/148‑1 (MQ=255)
                                                                                                                  tCCTGATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAg                              >  2:311278/1‑148 (MQ=255)
                                                                                                                      gATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCttt                          <  2:347465/148‑1 (MQ=255)
                                                                                                                      gATAGAGTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCttt                          <  2:645967/148‑1 (MQ=255)
                                                                                                                         agagTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGc                       >  2:2778415/1‑148 (MQ=255)
                                                                                                                         agagTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGc                       >  2:1025598/1‑148 (MQ=255)
                                                                                                                            gTTGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAg                    <  2:2182610/148‑1 (MQ=255)
                                                                                                                              tGATCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGaa                  >  2:764027/1‑148 (MQ=255)
                                                                                                                                aTCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACg                >  1:1664497/1‑148 (MQ=255)
                                                                                                                                aTCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACg                >  1:1512622/1‑148 (MQ=255)
                                                                                                                                aTCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACg                >  1:505210/1‑148 (MQ=255)
                                                                                                                                aTCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGCCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACg                >  1:1512641/1‑148 (MQ=255)
                                                                                                                                 tCGTCCAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACgg               <  2:1957461/148‑1 (MQ=255)
                                                                                                                                      cAGAGCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTgg          <  1:2492719/148‑1 (MQ=255)
                                                                                                                                       agagCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGt         <  2:3264809/148‑1 (MQ=255)
                                                                                                                                        gagCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTg        >  1:2133836/1‑148 (MQ=255)
                                                                                                                                        gagCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTg        >  2:1853938/1‑148 (MQ=255)
                                                                                                                                        gagCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTg        >  2:2288199/1‑148 (MQ=255)
                                                                                                                                         agCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGa       >  1:2386948/1‑148 (MQ=255)
                                                                                                                                         agCGACATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGa       >  2:1081146/1‑148 (MQ=255)
                                                                                                                                           cGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATc     >  1:3167933/1‑148 (MQ=255)
                                                                                                                                             aCATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCgt   <  1:505123/148‑1 (MQ=255)
                                                                                                                                              cATAGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCgtg  >  1:2330123/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCTCTTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTTGCCAGAACGGTTTGGTGATCGTG  >  CP009273/4590994‑4591283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑