Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,617,341 C→A 100% F70L (TTC→TTA)  nadR → trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,617,3410CA99.5% 648.4 / ‑4.3 196F70L (TTC→TTA) nadRtrifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (107/88);  total (107/89)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 196 total aligned reads displayed.

GATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCC  >  CP009273/4617198‑4617484
                                                                                                                                               |                                                                                                                                               
gATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTa                                                                                                                                             >  2:3023647/1‑148 (MQ=255)
gATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTa                                                                                                                                             <  1:3109912/148‑1 (MQ=255)
       gCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTa                                                                                                                                      >  2:34597/1‑148 (MQ=255)
           tATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCa                                                                                                                                  >  2:1794384/1‑148 (MQ=255)
           tATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCa                                                                                                                                  <  1:1804239/148‑1 (MQ=255)
            aTGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAACAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCAc                                                                                                                                 <  2:458017/148‑1 (MQ=255)
            aTGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCAc                                                                                                                                 >  1:2909851/1‑148 (MQ=255)
            aTGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCAc                                                                                                                                 <  2:1187901/148‑1 (MQ=255)
            aTGACCAAAGGGTATTTAAGCCAGTTACTGAATGACAAAATCAAAAGCCCCAGCGCGCAAAATCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCAc                                                                                                                                 >  1:2322217/1‑148 (MQ=255)
              gACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTg                                                                                                                               >  2:2840267/1‑148 (MQ=255)
              gACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTg                                                                                                                               <  1:2456744/148‑1 (MQ=255)
               aCCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGc                                                                                                                              >  2:448258/1‑148 (MQ=255)
                ccAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCa                                                                                                                             >  1:1399891/1‑148 (MQ=255)
                 cAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCAt                                                                                                                            >  1:2936584/1‑148 (MQ=255)
                 cAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCAt                                                                                                                            >  2:2642276/1‑148 (MQ=255)
                      ggTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCgg                                                                                                                       >  1:895473/1‑148 (MQ=255)
                          tttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACat                                                                                                                   <  1:2960799/148‑1 (MQ=255)
                           ttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACata                                                                                                                  >  1:2979945/1‑148 (MQ=255)
                             aaGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATc                                                                                                                >  1:1526520/1‑148 (MQ=255)
                             aaGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATc                                                                                                                <  2:53910/148‑1 (MQ=255)
                              aGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCt                                                                                                               <  1:1067066/148‑1 (MQ=255)
                               gCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTa                                                                                                              >  1:3358749/1‑148 (MQ=255)
                               gCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTa                                                                                                              >  1:2522787/1‑148 (MQ=255)
                                ccAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTAc                                                                                                             >  2:2734729/1‑148 (MQ=255)
                                 cAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTAcc                                                                                                            >  1:1760993/1‑148 (MQ=255)
                                 cAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTAcc                                                                                                            <  1:104612/148‑1 (MQ=255)
                                   gTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCtt                                                                                                          <  1:196069/148‑1 (MQ=255)
                                   gTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCtt                                                                                                          >  2:1624852/1‑148 (MQ=255)
                                   gTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCtt                                                                                                          >  2:856197/1‑148 (MQ=255)
                                   gTTACTGAATGCAAAAATCAAACGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCtt                                                                                                          <  1:3231771/148‑1 (MQ=255)
                                     tACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTAt                                                                                                        <  1:368369/148‑1 (MQ=255)
                                     tACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTAt                                                                                                        <  1:3125778/148‑1 (MQ=255)
                                      aCTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATc                                                                                                       <  2:3210304/148‑1 (MQ=255)
                                         gAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACCTATCTACCTTATCCAg                                                                                                    >  1:1950937/1‑148 (MQ=255)
                                              ccAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAgcgcgc                                                                                               >  1:1459305/1‑148 (MQ=255)
                                               cAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGcc                                                                                              <  1:2268254/148‑1 (MQ=255)
                                                 aaaTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTg                                                                                            <  2:417645/148‑1 (MQ=255)
                                                  aaTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGt                                                                                           <  1:964047/148‑1 (MQ=255)
                                                  aaTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGt                                                                                           <  1:1336678/148‑1 (MQ=255)
                                                    tCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAg                                                                                         >  1:1243899/1‑148 (MQ=255)
                                                     cAAAAGCCCCCGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGc                                                                                        <  1:793913/148‑1 (MQ=255)
                                                      aaaaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGTAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGcc                                                                                       >  2:612893/1‑148 (MQ=255)
                                                       aaaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCa                                                                                      <  1:710091/148‑1 (MQ=255)
                                                       aaaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCa                                                                                      >  1:2868741/1‑148 (MQ=255)
                                                         aGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAgg                                                                                    <  1:1645193/148‑1 (MQ=255)
                                                         aGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAgg                                                                                    >  2:2532167/1‑148 (MQ=255)
                                                         aGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAgg                                                                                    <  1:2058310/148‑1 (MQ=255)
                                                              cAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGAc                                                                               >  1:2975042/1‑148 (MQ=255)
                                                                 cgcgcAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAg                                                                            <  1:1656677/148‑1 (MQ=255)
                                                                  gcgcAACAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGc                                                                           <  2:1995037/148‑1 (MQ=255)
                                                                  gcgcAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGc                                                                           <  2:1416355/148‑1 (MQ=255)
                                                                       aaaGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCat                                                                      >  1:1685381/1‑148 (MQ=255)
                                                                        aaGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCata                                                                     >  2:480385/1‑148 (MQ=255)
                                                                        aaGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCata                                                                     >  1:283492/1‑148 (MQ=255)
                                                                          gCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATc                                                                   >  2:2971788/1‑148 (MQ=255)
                                                                               aGGCGTTGCACCGTTTTTTGGGGCTTGTGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTAt                                                              <  1:2351857/148‑1 (MQ=255)
                                                                                    ttGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGtt                                                         <  1:2996922/148‑1 (MQ=255)
                                                                                    ttGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGtt                                                         >  2:2117679/1‑148 (MQ=255)
                                                                                    ttGCACCGTTTTTTGGGGCTTGAGTGTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGtt                                                         <  1:1488520/148‑1 (MQ=255)
                                                                                     tGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGttt                                                        >  2:2291916/1‑148 (MQ=255)
                                                                                     tGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGttt                                                        >  2:2963890/1‑148 (MQ=255)
                                                                                     tGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGttt                                                        <  2:478543/148‑1 (MQ=255)
                                                                                       cACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTg                                                      <  2:2749967/148‑1 (MQ=255)
                                                                                       cACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTg                                                      <  2:3171066/148‑1 (MQ=255)
                                                                                       cACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTg                                                      <  2:3236268/148‑1 (MQ=255)
                                                                                        aCCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGa                                                     <  1:1967953/148‑1 (MQ=255)
                                                                                         ccGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGAc                                                    >  1:2980862/1‑148 (MQ=255)
                                                                                          cGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACg                                                   >  2:1884467/1‑148 (MQ=255)
                                                                                            ttttttGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGAt                                                 >  2:3086732/1‑148 (MQ=255)
                                                                                             tttttGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATa                                                <  1:1624835/148‑1 (MQ=255)
                                                                                             tttttGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATa                                                <  2:1600604/148‑1 (MQ=255)
                                                                                               tttGGGGCTTGAGTTTCCCCGGCGGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATAcc                                              >  1:2774049/1‑148 (MQ=255)
                                                                                               tttGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATAcc                                              >  2:1045176/1‑148 (MQ=255)
                                                                                                           gTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACcgcgcg                                  >  1:1478759/1‑148 (MQ=255)
                                                                                                           gTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACcgcgcg                                  <  1:1156311/148‑1 (MQ=255)
                                                                                                           gTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACcgcgcg                                  <  1:1876976/148‑1 (MQ=255)
                                                                                                           gTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACcgcgcg                                  <  1:2800668/148‑1 (MQ=255)
                                                                                                              tCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCgttg                               <  2:1587648/148‑1 (MQ=255)
                                                                                                              tCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCgttg                               <  2:3329325/148‑1 (MQ=255)
                                                                                                              tCCCCGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCgttg                               <  2:2898408/148‑1 (MQ=255)
                                                                                                                cccGGCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCGACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCgttgtt                             >  1:661761/1‑148 (MQ=255)
                                                                                                                   ggCAGAAGAAAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGa                          >  2:238831/1‑148 (MQ=255)
                                                                                                                   ggCAGAAGAAAACGATCGGTGTCGGATTAGGTACGGTCTACCCACTGCATACCGGACATCTCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATCTCATGATGGGTTTTGACGATACCCGTGACCGCGCGGTGTTCGa                          >  2:1315316/1‑148 (MQ=255)
                                                                                                                       gaagaaAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGAc                      >  1:1696573/1‑148 (MQ=255)
                                                                                                                        aagaaAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACa                     >  2:2046188/1‑148 (MQ=255)
                                                                                                                        aagaaAACGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACa                     >  1:2603048/1‑148 (MQ=255)
                                                                                                                              aCGATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGGTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCa               >  2:2760772/1‑148 (MQ=255)
                                                                                                                                gATCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATg             <  1:128563/148‑1 (MQ=255)
                                                                                                                                 aTCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGt            <  1:2046188/148‑1 (MQ=255)
                                                                                                                                  tCGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTc           >  2:810990/1‑148 (MQ=255)
                                                                                                                                   cGGTGTCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCg          <  2:1399887/148‑1 (MQ=255)
                                                                                                                                     gtgtCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgca        >  1:85735/1‑148 (MQ=255)
                                                                                                                                     gtgtCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgca        >  2:1419920/1‑148 (MQ=255)
                                                                                                                                     gtgtCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgca        <  2:1034190/148‑1 (MQ=255)
                                                                                                                                       gtCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgcagc      >  2:329556/1‑148 (MQ=255)
                                                                                                                                       gtCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgcagc      >  2:2575681/1‑148 (MQ=255)
                                                                                                                                       gtCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgcagc      >  1:2755221/1‑148 (MQ=255)
                                                                                                                                       gtCGTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgcagc      >  2:990225/1‑148 (MQ=255)
                                                                                                                                          gTATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCagcagc   >  2:3187969/1‑148 (MQ=255)
                                                                                                                                           tATTAGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGcc  >  2:1536563/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCC  >  CP009273/4617198‑4617484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑