Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,630,770 G→T 100% T4T (ACG→ACT)  yjtD → putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,630,7700GT100.0% 739.7 / ‑6.7 228T4T (ACG→ACT) yjtDputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (122/105);  total (123/105)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 230 total aligned reads displayed.

GAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGT  >  CP009273/4630623‑4630914
                                                                                                                                                   |                                                                                                                                                
gAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACg                                                                                                                                                  >  2:2524091/1‑148 (MQ=255)
  aaTAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTat                                                                                                                                                <  1:2593209/148‑1 (MQ=255)
  aaTAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTat                                                                                                                                                >  1:1810044/1‑148 (MQ=255)
   aTAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTatt                                                                                                                                               <  2:1663688/148‑1 (MQ=255)
    tAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTatta                                                                                                                                              >  2:896932/1‑148 (MQ=255)
    tAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTatta                                                                                                                                              <  2:1147856/148‑1 (MQ=255)
      gTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTattatt                                                                                                                                            <  2:1788884/148‑1 (MQ=255)
      gTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTattatt                                                                                                                                            <  2:835407/148‑1 (MQ=255)
           gCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGt                                                                                                                                       >  1:3046150/1‑148 (MQ=255)
           gCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGt                                                                                                                                       >  2:792661/1‑148 (MQ=255)
            ccTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTc                                                                                                                                      >  1:2963001/1‑148 (MQ=255)
              ttttttAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGc                                                                                                                                    <  2:2435511/148‑1 (MQ=255)
                ttttAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCAc                                                                                                                                  >  1:718215/1‑148 (MQ=255)
                 tttAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCAcc                                                                                                                                 <  1:574634/148‑1 (MQ=255)
                   tAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCg                                                                                                                               <  2:3203352/148‑1 (MQ=255)
                     aGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGcc                                                                                                                             >  1:1390076/1‑148 (MQ=255)
                     aGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGcc                                                                                                                             <  2:1453988/148‑1 (MQ=255)
                     aGCGAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCACGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGcc                                                                                                                             >  2:2930545/1‑148 (MQ=255)
                      gCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCa                                                                                                                            >  1:825180/1‑148 (MQ=255)
                       cTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCag                                                                                                                           <  1:2964049/148‑1 (MQ=255)
                          aGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATACCTATTATTCTGGTCGCACCCGCCCGAGc                                                                                                                        >  2:2861842/1‑148 (MQ=255)
                           gTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCa                                                                                                                       >  2:634873/1‑148 (MQ=255)
                           gTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCa                                                                                                                       >  2:2590150/1‑148 (MQ=255)
                           gTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCa                                                                                                                       >  2:1089986/1‑148 (MQ=255)
                           gTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCa                                                                                                                       >  2:1180055/1‑148 (MQ=255)
                           gTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCa                                                                                                                       >  2:677449/1‑148 (MQ=255)
                            tAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAg                                                                                                                      <  2:2999228/148‑1 (MQ=255)
                            tAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAg                                                                                                                      >  2:3475222/1‑148 (MQ=255)
                               aGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGaaa                                                                                                                   <  1:2906068/148‑1 (MQ=255)
                               aGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGaaa                                                                                                                   <  1:1957790/148‑1 (MQ=255)
                               aGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGaaa                                                                                                                   <  2:1810003/148‑1 (MQ=255)
                               aGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGaaa                                                                                                                   >  1:47283/1‑148 (MQ=255)
                                   cTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAatat                                                                                                               <  2:740395/148‑1 (MQ=255)
                                      tttCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTgg                                                                                                            <  1:2833548/148‑1 (MQ=255)
                                       ttCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTggg                                                                                                           <  2:3093402/148‑1 (MQ=255)
                                       ttCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTggg                                                                                                           <  2:2500590/148‑1 (MQ=255)
                                        tCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTgggg                                                                                                          >  1:2829753/1‑148 (MQ=255)
                                           gCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAg                                                                                                       >  2:3146988/1‑148 (MQ=255)
                                                    gTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCggg                                                                                              >  1:1164485/1‑148 (MQ=255)
                                                     ttAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGc                                                                                             >  2:3252980/1‑148 (MQ=255)
                                                     ttAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGc                                                                                             >  2:2087595/1‑148 (MQ=255)
                                                        aGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATAGGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAAt                                                                                          <  2:3307337/148‑1 (MQ=255)
                                                          aaTTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGa                                                                                        <  1:2693875/148‑1 (MQ=255)
                                                          aaTTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGa                                                                                        >  2:896162/1‑148 (MQ=255)
                                                            tttGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGaaa                                                                                      >  2:273039/1‑148 (MQ=255)
                                                             ttGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGaaaa                                                                                     >  2:231097/1‑148 (MQ=255)
                                                             ttGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGaaaa                                                                                     >  2:1368430/1‑148 (MQ=255)
                                                                 aaaTTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAt                                                                                 >  2:2306779/1‑148 (MQ=255)
                                                                   aTTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATgg                                                                               >  1:1095839/1‑148 (MQ=255)
                                                                     tCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATgggg                                                                             <  2:503155/148‑1 (MQ=255)
                                                                     tCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATgggg                                                                             <  1:1749187/148‑1 (MQ=255)
                                                                     tCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATgggg                                                                             >  2:3404223/1‑148 (MQ=255)
                                                                      cGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGTTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGt                                                                            <  1:917219/148‑1 (MQ=255)
                                                                      cGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGt                                                                            >  2:1588249/1‑148 (MQ=255)
                                                                      cGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGt                                                                            >  2:3329126/1‑148 (MQ=255)
                                                                        cACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAGGGGGttt                                                                          >  1:196967/1‑148 (MQ=255)
                                                                         aCCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTa                                                                         >  2:1413325/1‑148 (MQ=255)
                                                                              gTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGAt                                                                    <  2:308260/148‑1 (MQ=255)
                                                                              gTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGAt                                                                    <  2:1231076/148‑1 (MQ=255)
                                                                               taataaGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATc                                                                   <  2:3241476/148‑1 (MQ=255)
                                                                                aataaGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCt                                                                  >  1:2452921/1‑148 (MQ=255)
                                                                                  taaGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGc                                                                <  1:1437850/148‑1 (MQ=255)
                                                                                   aaGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCg                                                               <  1:1180055/148‑1 (MQ=255)
                                                                                    aGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCgg                                                              <  2:1456923/148‑1 (MQ=255)
                                                                                     gTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGa                                                             <  1:1353354/148‑1 (MQ=255)
                                                                                          cAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTc                                                        >  1:1261936/1‑148 (MQ=255)
                                                                                             tGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGAt                                                     <  2:718023/148‑1 (MQ=255)
                                                                                             tGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAAATATTATTCTGTTCGCACCCGCCATAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGAt                                                     <  1:13698/148‑1 (MQ=255)
                                                                                              gATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATa                                                    >  1:704351/1‑148 (MQ=255)
                                                                                               aTCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCTGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAg                                                   <  1:1654681/148‑1 (MQ=255)
                                                                                                 cACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTc                                                 <  1:1495465/148‑1 (MQ=255)
                                                                                                 cACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTc                                                 <  1:3475222/148‑1 (MQ=255)
                                                                                                   cccGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCACTGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAg                                               >  1:3108684/1‑148 (MQ=255)
                                                                                                   cccGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAg                                               >  1:266235/1‑148 (MQ=255)
                                                                                                   cccGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAg                                               >  1:3108600/1‑148 (MQ=255)
                                                                                                        ttCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGcaca                                          >  1:2796117/1‑148 (MQ=255)
                                                                                                         tCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCacac                                         <  2:1961909/148‑1 (MQ=255)
                                                                                                          cgcgGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACAcc                                        <  2:1844549/148‑1 (MQ=255)
                                                                                                          cgcgGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACAcc                                        <  2:2963001/148‑1 (MQ=255)
                                                                                                                tATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGagc                                  >  1:2214061/1‑148 (MQ=255)
                                                                                                                tATTTGATCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGagc                                  >  2:1346387/1‑148 (MQ=255)
                                                                                                                      aTCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAgccagcca                            >  1:308324/1‑148 (MQ=255)
                                                                                                                      aTCAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAgccagcca                            >  1:2081382/1‑148 (MQ=255)
                                                                                                                        cAAGAAGAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCAcc                          <  1:3408175/148‑1 (MQ=255)
                                                                                                                         aagaagAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCAccc                         >  2:2199555/1‑148 (MQ=255)
                                                                                                                         aagaagAGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCAccc                         >  2:697396/1‑148 (MQ=255)
                                                                                                                            aagaGTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCt                      >  2:1766069/1‑148 (MQ=255)
                                                                                                                             agagTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTg                     >  2:506763/1‑148 (MQ=255)
                                                                                                                               agTGGCAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTggg                   >  2:2047235/1‑148 (MQ=255)
                                                                                                                                    cAATATGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGca              >  2:2547445/1‑148 (MQ=255)
                                                                                                                                      atatGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGcaca            <  2:2187590/148‑1 (MQ=255)
                                                                                                                                         tGCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATgg         <  1:3218234/148‑1 (MQ=255)
                                                                                                                                          gCGTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGa        <  2:937085/148‑1 (MQ=255)
                                                                                                                                            gTATAACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATc      >  1:378220/1‑148 (MQ=255)
                                                                                                                                             tataACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCt     >  2:2135963/1‑148 (MQ=255)
                                                                                                                                              ataACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTg    >  1:3306214/1‑148 (MQ=255)
                                                                                                                                              ataACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTg    >  2:1872630/1‑148 (MQ=255)
                                                                                                                                              ataACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTg    <  1:2930556/148‑1 (MQ=255)
                                                                                                                                               taACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTgg   >  1:1483811/1‑148 (MQ=255)
                                                                                                                                               taACTATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTgg   <  1:896527/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTATTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGT  >  CP009273/4630623‑4630914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑