Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 372,091 C→A 100% R393R (CGG→AGG)  mhpT → 3‑hydroxyphenylpropionic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273372,0910CA98.6% 479.5 / ‑1.3 153R393R (CGG→AGG) mhpT3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base A (68/82);  total (71/82)
Fisher's exact test for biased strand distribution p-value = 9.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 153 total aligned reads displayed.

CGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGG  >  CP009273/371946‑372231
                                                                                                                                                 |                                                                                                                                            
 gTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGga                                                                                                                                           <  2:3099823/148‑1 (MQ=255)
  tGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGgag                                                                                                                                          <  2:1109303/148‑1 (MQ=255)
  tGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGgag                                                                                                                                          >  2:2917930/1‑148 (MQ=255)
    gCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGAt                                                                                                                                        >  2:3502256/1‑148 (MQ=255)
      ggTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCa                                                                                                                                      <  2:1575520/148‑1 (MQ=255)
          gggCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGaa                                                                                                                                  <  2:1107199/148‑1 (MQ=255)
          gggCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGaa                                                                                                                                  >  2:1982916/1‑148 (MQ=255)
           ggCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAAt                                                                                                                                 >  1:1838527/1‑148 (MQ=255)
                cTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGc                                                                                                                            <  1:1403187/148‑1 (MQ=255)
                     ggCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTgc                                                                                                                       >  2:2060367/1‑148 (MQ=255)
                     ggCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTgc                                                                                                                       >  2:2141113/1‑148 (MQ=255)
                      gCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTgcg                                                                                                                      <  1:3105502/148‑1 (MQ=255)
                         aTGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCg                                                                                                                   >  2:1781543/1‑148 (MQ=255)
                         aTGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCg                                                                                                                   <  2:449908/148‑1 (MQ=255)
                         aTGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCg                                                                                                                   <  2:449896/148‑1 (MQ=255)
                            aGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATg                                                                                                                >  2:143615/1‑148 (MQ=255)
                              cGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGcc                                                                                                              >  1:2057478/1‑148 (MQ=255)
                              cGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGcc                                                                                                              >  1:2057493/1‑148 (MQ=255)
                                  ccGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGGTGGGTTGGCGGTGTTTATTTTGATTAGCAGGAGTTCACGAATACAGCCGTGCGCCGATGCCTGAc                                                                                                          >  1:1519902/1‑148 (MQ=255)
                                    gTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGt                                                                                                        >  2:245867/1‑148 (MQ=255)
                                           gCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCttat                                                                                                 >  2:2732008/1‑148 (MQ=255)
                                           gCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCttat                                                                                                 >  1:3482865/1‑148 (MQ=255)
                                           gCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGGGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCttat                                                                                                 >  1:1906955/1‑148 (MQ=255)
                                            ccGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCttatt                                                                                                <  1:1229263/148‑1 (MQ=255)
                                               ggAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAgg                                                                                             >  2:3151263/1‑148 (MQ=255)
                                               ggAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAgg                                                                                             <  1:986708/148‑1 (MQ=255)
                                               ggAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGAGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAgg                                                                                             <  1:436811/148‑1 (MQ=255)
                                                gAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGc                                                                                            >  2:666826/1‑148 (MQ=255)
                                                 aaaaTGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCa                                                                                           <  2:1920045/148‑1 (MQ=255)
                                                     tGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAgggg                                                                                       <  1:2175883/148‑1 (MQ=255)
                                                         ggCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGaa                                                                                   <  2:924770/148‑1 (MQ=255)
                                                             ttAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAggg                                                                               <  1:1618851/148‑1 (MQ=255)
                                                              tAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGa                                                                              <  2:1408240/148‑1 (MQ=255)
                                                               aGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGAc                                                                             <  2:1358382/148‑1 (MQ=255)
                                                               aGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTAGTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGAc                                                                             <  1:2939932/148‑1 (MQ=255)
                                                                ggCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACt                                                                            <  2:2044723/148‑1 (MQ=255)
                                                                   aCTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCa                                                                         <  2:2235442/148‑1 (MQ=255)
                                                                      ggCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCAccc                                                                      <  2:3282522/148‑1 (MQ=255)
                                                                        cACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCa                                                                    <  1:419882/148‑1 (MQ=255)
                                                                        cACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCa                                                                    <  2:2894370/148‑1 (MQ=255)
                                                                           ggTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATa                                                                 >  1:1307970/1‑148 (MQ=255)
                                                                            gTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATac                                                                <  2:2502712/148‑1 (MQ=255)
                                                                             tCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATaca                                                               <  1:362909/148‑1 (MQ=255)
                                                                               ggCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACAcc                                                             <  1:69742/148‑1 (MQ=255)
                                                                                gCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCt                                                            <  1:943940/148‑1 (MQ=255)
                                                                                 cGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTa                                                           >  2:1261880/1‑148 (MQ=255)
                                                                                 cGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTa                                                           <  1:1216401/148‑1 (MQ=255)
                                                                                 cGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTa                                                           >  1:137207/1‑148 (MQ=255)
                                                                                 cGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTa                                                           >  2:3011093/1‑148 (MQ=255)
                                                                                    aaTGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACgg                                                        >  1:3357065/1‑148 (MQ=255)
                                                                                     aTGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCTCGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACGCCAATACACCTACGGc                                                       >  2:1974473/1‑148 (MQ=255)
                                                                                     aTGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGc                                                       >  2:631530/1‑148 (MQ=255)
                                                                                     aTGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGc                                                       >  2:1974535/1‑148 (MQ=255)
                                                                                          cGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTa                                                  >  1:3264568/1‑148 (MQ=255)
                                                                                              tCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAgg                                              <  2:105737/148‑1 (MQ=255)
                                                                                              tCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAgg                                              >  1:1065876/1‑148 (MQ=255)
                                                                                              tCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAgg                                              >  1:1066000/1‑148 (MQ=255)
                                                                                              tCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTAAGGCGTTTACAgg                                              >  1:3380065/1‑148 (MQ=255)
                                                                                               cTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGt                                             <  1:2473892/148‑1 (MQ=255)
                                                                                               cTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGt                                             <  1:71809/148‑1 (MQ=255)
                                                                                                tGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGta                                            <  2:1347815/148‑1 (MQ=255)
                                                                                                  cACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGtata                                          >  2:2172176/1‑148 (MQ=255)
                                                                                                    ccGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACt                                        >  2:32444/1‑148 (MQ=255)
                                                                                                    ccGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACt                                        >  1:860469/1‑148 (MQ=255)
                                                                                                       ggTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGc                                     <  1:2757822/148‑1 (MQ=255)
                                                                                                           ttCTTGTTTCTGTGTTGGATTTTTTTTTTTTGATGAGCAGGAGATCACTAATACATCCGTGCGCCGATGCCTGACTTGACTTATTATGCAGTGGAGTTAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATTCTAGCttat                                 <  2:2372235/148‑4 (MQ=255)
                                                                                                           ttCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaa                                 >  1:2254387/1‑148 (MQ=255)
                                                                                                           ttCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaa                                 >  1:2661406/1‑148 (MQ=255)
                                                                                                            tCTTGTTGCTTGGTTGGCGGTGTTTATTTTGATGAGCATTAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCATTATTAGGCAGTGGCGAAAGGGACGTCACCCCAATACACATAAGGCGTTTACATGTATACTCGCTaaaa                                >  2:3203540/1‑148 (MQ=255)
                                                                                                            tCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaaa                                <  1:936495/148‑1 (MQ=255)
                                                                                                             cTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaaaa                               <  1:974727/148‑1 (MQ=255)
                                                                                                               tgttgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAatt                             <  2:2767599/148‑1 (MQ=255)
                                                                                                                 ttgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAattat                           <  2:755462/148‑1 (MQ=255)
                                                                                                                  tgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAattatt                          <  1:3094782/148‑1 (MQ=255)
                                                                                                                  tgCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAattatt                          <  1:1700461/148‑1 (MQ=255)
                                                                                                                   gCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTc                         >  1:3502807/1‑148 (MQ=255)
                                                                                                                   gCTGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTc                         >  1:1762765/1‑148 (MQ=255)
                                                                                                                     tGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAg                       <  1:283907/148‑1 (MQ=255)
                                                                                                                     tGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAg                       >  2:3087314/1‑148 (MQ=255)
                                                                                                                     tGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAg                       >  2:3087319/1‑148 (MQ=255)
                                                                                                                     tGGGTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAg                       <  2:1664944/148‑1 (MQ=255)
                                                                                                                       ggTTGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCg                     >  2:3353485/1‑148 (MQ=255)
                                                                                                                          tGGCGGTGTTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGt                  <  2:1655190/148‑1 (MQ=255)
                                                                                                                               gtgtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCTCTAAAAATTATTCAGCGGGTTTGGa             >  1:3016714/1‑148 (MQ=255)
                                                                                                                                tgtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaa            <  2:1461883/148‑1 (MQ=255)
                                                                                                                                tgtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaa            <  1:1568800/148‑1 (MQ=255)
                                                                                                                                tgtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaa            <  1:1568951/148‑1 (MQ=255)
                                                                                                                                tgtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaa            >  1:1682412/1‑148 (MQ=255)
                                                                                                                                tgtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaa            >  1:2378601/1‑148 (MQ=255)
                                                                                                                                tgtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaa            <  2:264830/148‑1 (MQ=255)
                                                                                                                                tgtTTATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaa            <  1:3309052/148‑1 (MQ=255)
                                                                                                                                  tttATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAAc          >  2:3444921/1‑148 (MQ=255)
                                                                                                                                  tttATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAAc          >  1:590370/1‑148 (MQ=255)
                                                                                                                                  tttATTTTGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAAc          >  1:590232/1‑148 (MQ=255)
                                                                                                                                   ttATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACa         >  1:1868698/1‑148 (MQ=255)
                                                                                                                                   ttATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACa         >  1:89630/1‑148 (MQ=255)
                                                                                                                                       tttGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGTCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGa     >  1:2729454/1‑148 (MQ=255)
                                                                                                                                       tttGATGAGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGa     <  2:748295/148‑1 (MQ=255)
                                                                                                                                         tgatgaGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATg   <  2:3482865/148‑1 (MQ=255)
                                                                                                                                          gatgaGCAGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATgg  <  2:1906955/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
CGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGG  >  CP009273/371946‑372231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑