Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 390,551 T→G 100% L197R (CTG→CGG)  yaiV → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273390,5510TG98.4% 819.7 / ‑2.1 203L197R (CTG→CGG) yaiVputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base G (96/103);  total (99/104)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

ATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCA  >  CP009273/390406‑390695
                                                                                                                                                 |                                                                                                                                                
 tGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGt                                                                                                                                               >  2:872775/1‑148 (MQ=255)
   aTTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCg                                                                                                                                             <  2:389825/148‑1 (MQ=255)
     tGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCt                                                                                                                                           <  2:1660864/148‑1 (MQ=255)
        cTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATc                                                                                                                                        >  2:205647/1‑148 (MQ=255)
        cTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCATAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATc                                                                                                                                        >  2:2537067/1‑148 (MQ=255)
         tGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCa                                                                                                                                       <  2:1461575/148‑1 (MQ=255)
           gAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAAc                                                                                                                                     >  1:2720416/1‑148 (MQ=255)
           gAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAAc                                                                                                                                     <  2:2526404/148‑1 (MQ=255)
            aaTGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAAcc                                                                                                                                    >  2:2088703/1‑148 (MQ=255)
             aTGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCg                                                                                                                                   >  1:2939341/1‑148 (MQ=255)
             aTGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCg                                                                                                                                   <  1:841567/148‑1 (MQ=255)
              tGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGt                                                                                                                                  <  2:2233936/148‑1 (MQ=255)
              tGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGt                                                                                                                                  >  1:736801/1‑148 (MQ=255)
                 agaaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTCTCAACCGTTTg                                                                                                                               >  2:3214031/1‑148 (MQ=255)
                 agaaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTg                                                                                                                               <  1:566822/148‑1 (MQ=255)
                 agaaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTg                                                                                                                               >  2:3408777/1‑148 (MQ=255)
                  gaaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGc                                                                                                                              <  1:1516492/148‑1 (MQ=255)
                  gaaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGc                                                                                                                              <  1:1814276/148‑1 (MQ=255)
                   aaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATCACGGCACACGGGTCGCTATCAACCGTTTGcc                                                                                                                             <  1:1584116/148‑1 (MQ=255)
                   aaTTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGcc                                                                                                                             >  1:2863336/1‑148 (MQ=255)
                     ttGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCtt                                                                                                                           >  1:2191239/1‑148 (MQ=255)
                     ttGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCtt                                                                                                                           >  1:2191462/1‑148 (MQ=255)
                         gATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCaga                                                                                                                       >  2:602466/1‑148 (MQ=255)
                          aTCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCagag                                                                                                                      >  1:127883/1‑148 (MQ=255)
                          aTCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCagag                                                                                                                      >  1:2815547/1‑148 (MQ=255)
                            cACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTa                                                                                                                    >  1:2528912/1‑148 (MQ=255)
                            cACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTa                                                                                                                    >  1:3081771/1‑148 (MQ=255)
                            cACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTa                                                                                                                    <  2:2330453/148‑1 (MQ=255)
                             aCGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTAt                                                                                                                   <  2:3459507/148‑1 (MQ=255)
                             aCGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTAt                                                                                                                   >  2:24031/1‑148 (MQ=255)
                              cGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTAtt                                                                                                                  >  2:3260186/1‑148 (MQ=255)
                                tATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTaa                                                                                                                <  2:1357018/148‑1 (MQ=255)
                                 aTTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAAt                                                                                                               >  1:3093899/1‑148 (MQ=255)
                                   tGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCa                                                                                                             >  1:206495/1‑148 (MQ=255)
                                     gtgtGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAgg                                                                                                           <  2:2959421/148‑1 (MQ=255)
                                      tgtgATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGa                                                                                                          >  2:536469/1‑148 (MQ=255)
                                         gatgaATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGAcgc                                                                                                       >  1:1164939/1‑148 (MQ=255)
                                         gatgaATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGAcgc                                                                                                       >  1:1724362/1‑148 (MQ=255)
                                           tgaATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCgg                                                                                                     <  1:26066/148‑1 (MQ=255)
                                             aaTTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCgggg                                                                                                   <  2:3496808/148‑1 (MQ=255)
                                               ttATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGAt                                                                                                 >  2:1755130/1‑148 (MQ=255)
                                                tataTCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATa                                                                                                >  2:2010857/1‑148 (MQ=255)
                                                tataTCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATa                                                                                                <  2:2933953/148‑1 (MQ=255)
                                                    tCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCa                                                                                            <  2:3121075/148‑1 (MQ=255)
                                                    tCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCa                                                                                            >  1:1320994/1‑148 (MQ=255)
                                                     ccATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCaa                                                                                           >  1:2873279/1‑148 (MQ=255)
                                                     ccATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCaa                                                                                           <  1:2135925/148‑1 (MQ=255)
                                                        tCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACgg                                                                                        <  1:1710527/148‑1 (MQ=255)
                                                        tCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACgg                                                                                        <  2:1327957/148‑1 (MQ=255)
                                                         cAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGt                                                                                       >  2:604563/1‑148 (MQ=255)
                                                          aaCGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCTTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGtt                                                                                      <  2:206936/148‑1 (MQ=255)
                                                            cGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTa                                                                                    >  1:322517/1‑148 (MQ=255)
                                                              tACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTAtt                                                                                  <  2:1516957/148‑1 (MQ=255)
                                                               acacGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTc                                                                                 <  1:3175822/148‑1 (MQ=255)
                                                                cacGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTcc                                                                                >  1:1346075/1‑148 (MQ=255)
                                                                   gCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGAGGCGGGGATAACCAACGGTTTATTCCCGc                                                                             >  2:3499391/1‑148 (MQ=255)
                                                                    cATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCt                                                                            <  2:3384812/148‑1 (MQ=255)
                                                                     atatCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCtt                                                                           >  2:693082/1‑148 (MQ=255)
                                                                     atatCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCtt                                                                           <  1:489214/148‑1 (MQ=255)
                                                                       atCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAg                                                                         <  1:3342184/148‑1 (MQ=255)
                                                                        tCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGc                                                                        >  1:2231138/1‑148 (MQ=255)
                                                                        tCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGc                                                                        >  1:2908700/1‑148 (MQ=255)
                                                                         cgcgTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCt                                                                       >  1:53096/1‑148 (MQ=255)
                                                                            gTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCgg                                                                    <  1:1252546/148‑1 (MQ=255)
                                                                              tCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTc                                                                  >  2:1922144/1‑148 (MQ=255)
                                                                                 gtcgtcGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCAcc                                                               >  2:1712519/1‑148 (MQ=255)
                                                                                  tcgtcgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCa                                                              >  1:2469157/1‑148 (MQ=255)
                                                                                   cgtcgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAg                                                             >  2:1656933/1‑148 (MQ=255)
                                                                                      cgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGACATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTc                                                          <  2:1704631/148‑1 (MQ=255)
                                                                                      cgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTc                                                          >  1:1035965/1‑148 (MQ=255)
                                                                                         aGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCAtt                                                       >  1:2036013/1‑148 (MQ=255)
                                                                                          gAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTg                                                      <  2:768574/148‑1 (MQ=255)
                                                                                          gAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTg                                                      <  1:430043/148‑1 (MQ=255)
                                                                                           aaGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGa                                                     >  2:1948412/1‑148 (MQ=255)
                                                                                              ttCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGc                                                  >  1:639988/1‑148 (MQ=255)
                                                                                                ctcGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCa                                                <  2:125680/148‑1 (MQ=255)
                                                                                                       ctTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCa                                         >  1:1977134/1‑148 (MQ=255)
                                                                                                         ttGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCata                                       >  1:3486396/1‑148 (MQ=255)
                                                                                                          tGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCatat                                      <  1:2342049/148‑1 (MQ=255)
                                                                                                              tAAAGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATAttttt                                  >  1:3208086/1‑148 (MQ=255)
                                                                                                               aaaGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTa                                 >  2:506360/1‑148 (MQ=255)
                                                                                                                aaGGCGGCTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTaa                                <  2:2752061/148‑1 (MQ=255)
                                                                                                                  ggcggcTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAg                              >  1:3443253/1‑148 (MQ=255)
                                                                                                                    cggcTATATCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAgcg                            <  2:1794815/148‑1 (MQ=255)
                                                                                                                          tatCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCaa                      <  1:988646/148‑1 (MQ=255)
                                                                                                                          tatCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCaa                      <  2:3245774/148‑1 (MQ=255)
                                                                                                                          tatCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCaa                      >  2:1413503/1‑148 (MQ=255)
                                                                                                                           atCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAAc                     <  1:1421306/148‑1 (MQ=255)
                                                                                                                            tCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACg                    <  2:2761779/148‑1 (MQ=255)
                                                                                                                            tCGAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACg                    >  1:3141687/1‑148 (MQ=255)
                                                                                                                              gAAATGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTATCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGc                  <  2:127883/148‑1 (MQ=255)
                                                                                                                                aaTGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGa                <  2:2190437/148‑1 (MQ=255)
                                                                                                                                aaTGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGa                <  1:420610/148‑1 (MQ=255)
                                                                                                                                aaTGAATAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGa                <  1:141018/148‑1 (MQ=255)
                                                                                                                                    aaTAAAGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGa            <  2:2856816/148‑1 (MQ=255)
                                                                                                                                        aaGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGa        <  1:24031/148‑1 (MQ=255)
                                                                                                                                         aGGCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGac       <  1:1135265/148‑1 (MQ=255)
                                                                                                                                          ggCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGacc      >  1:2551717/1‑148 (MQ=255)
                                                                                                                                           gCAAACGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGacca     >  2:2279442/1‑148 (MQ=255)
                                                                                                                                              aaCGGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGAccacca  >  2:4741/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCA  >  CP009273/390406‑390695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑