Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 402,340 G→T 100% V160V (GTG→GTT)  aroL → shikimate kinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273402,3400GT100.0% 777.7 / NA 233V160V (GTG→GTT) aroLshikimate kinase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (104/129);  total (104/129)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 233 total aligned reads displayed.

AGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGA  >  CP009273/402196‑402484
                                                                                                                                                |                                                                                                                                                
aGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTAtt                                                                                                                                               >  1:2668583/1‑148 (MQ=255)
  cTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTc                                                                                                                                             >  1:233707/1‑148 (MQ=255)
   tGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCt                                                                                                                                            <  1:960521/148‑1 (MQ=255)
    gCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTg                                                                                                                                           <  2:1330406/148‑1 (MQ=255)
      aCCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGaa                                                                                                                                         <  2:2226545/148‑1 (MQ=255)
           aagaagATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCg                                                                                                                                    >  2:1368050/1‑148 (MQ=255)
            agaagaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGc                                                                                                                                   >  2:931397/1‑148 (MQ=255)
              aagaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAg                                                                                                                                 <  1:1358672/148‑1 (MQ=255)
              aagaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAg                                                                                                                                 <  1:3491128/148‑1 (MQ=255)
               agaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAgc                                                                                                                                >  1:2614499/1‑148 (MQ=255)
                gaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAgcg                                                                                                                               >  2:2665731/1‑148 (MQ=255)
                gaTTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAgcg                                                                                                                               >  2:288031/1‑148 (MQ=255)
                      cGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTgg                                                                                                                         >  1:2702065/1‑148 (MQ=255)
                          cAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGcaca                                                                                                                     >  2:2922424/1‑148 (MQ=255)
                           aaCCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAg                                                                                                                    >  2:3235120/1‑148 (MQ=255)
                              cTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACg                                                                                                                 <  1:1049733/148‑1 (MQ=255)
                               ttAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGa                                                                                                                >  2:3344785/1‑148 (MQ=255)
                               ttAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGa                                                                                                                <  1:317167/148‑1 (MQ=255)
                               ttAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGa                                                                                                                <  1:2770156/148‑1 (MQ=255)
                                tAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGAt                                                                                                               <  2:1202487/148‑1 (MQ=255)
                                  aCGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCa                                                                                                             <  2:3441953/148‑1 (MQ=255)
                                  aCGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCa                                                                                                             >  2:460349/1‑148 (MQ=255)
                                  aCGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCa                                                                                                             >  2:2001607/1‑148 (MQ=255)
                                  aCGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCa                                                                                                             <  1:660355/148‑1 (MQ=255)
                                  aCGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCa                                                                                                             <  1:73897/148‑1 (MQ=255)
                                   ctggAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTTGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACTAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCaa                                                                                                            <  2:1983927/146‑1 (MQ=255)
                                       aaaaCCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAttgt                                                                                                        <  2:2817048/148‑1 (MQ=255)
                                        aaaCCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAttgtt                                                                                                       >  1:3444639/1‑148 (MQ=255)
                                        aaaCCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAAttgtt                                                                                                       >  2:1521359/1‑148 (MQ=255)
                                         aaCCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGTTCAAttgttg                                                                                                      <  2:2889397/148‑1 (MQ=255)
                                           ccGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGAt                                                                                                    >  1:132233/1‑148 (MQ=255)
                                             gCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGAttt                                                                                                  <  2:3449939/148‑1 (MQ=255)
                                                 aGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGa                                                                                              >  1:1635221/1‑148 (MQ=255)
                                                   cGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAgc                                                                                            >  2:197451/1‑148 (MQ=255)
                                                       gaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCt                                                                                        >  1:879985/1‑148 (MQ=255)
                                                       gaagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCt                                                                                        >  1:2621596/1‑148 (MQ=255)
                                                        aagTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCta                                                                                       >  2:1934950/1‑148 (MQ=255)
                                                             cAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACtt                                                                                  >  1:282780/1‑148 (MQ=255)
                                                              aGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTa                                                                                 <  1:617859/148‑1 (MQ=255)
                                                              aGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTa                                                                                 >  2:288549/1‑148 (MQ=255)
                                                               ggAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTaa                                                                                <  1:460349/148‑1 (MQ=255)
                                                               ggAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTaa                                                                                <  1:2001607/148‑1 (MQ=255)
                                                               ggAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTaa                                                                                <  2:714531/148‑1 (MQ=255)
                                                                  aGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGt                                                                             <  2:3226591/148‑1 (MQ=255)
                                                                  aGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGt                                                                             <  1:1705892/148‑1 (MQ=255)
                                                                    tGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTc                                                                           <  1:3443561/148‑1 (MQ=255)
                                                                     gCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCa                                                                          <  2:1230811/148‑1 (MQ=255)
                                                                      cTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCAt                                                                         <  1:1980649/148‑1 (MQ=255)
                                                                         gaagaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATccc                                                                      >  2:2745759/1‑148 (MQ=255)
                                                                         gaagaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATccc                                                                      >  2:2625076/1‑148 (MQ=255)
                                                                          aagaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCg                                                                     <  2:558985/148‑1 (MQ=255)
                                                                          aagaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCg                                                                     <  2:267993/148‑1 (MQ=255)
                                                                            gaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTg                                                                   <  2:2502138/148‑1 (MQ=255)
                                                                            gaaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTg                                                                   >  1:2417584/1‑148 (MQ=255)
                                                                             aaCGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGa                                                                  >  2:1059507/1‑148 (MQ=255)
                                                                               cgcgATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGaaa                                                                <  2:2859239/148‑1 (MQ=255)
                                                                                    tGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAgg                                                           <  1:1161599/148‑1 (MQ=255)
                                                                                       gcTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGgaag                                                        <  2:982591/148‑1 (MQ=255)
                                                                                          atatCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAAc                                                     >  1:2221005/1‑148 (MQ=255)
                                                                                           tatCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACg                                                    <  2:914417/148‑1 (MQ=255)
                                                                                            atCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGa                                                   <  2:1664035/148‑1 (MQ=255)
                                                                                            atCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGa                                                   >  1:1279011/1‑148 (MQ=255)
                                                                                              cgcgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATg                                                 <  2:1749116/148‑1 (MQ=255)
                                                                                               gcgAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGc                                                >  1:2827400/1‑148 (MQ=255)
                                                                                                 taaGTTGCGCATTTTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACTATCATTTGTTGATTTTCGAGCGCCTTTACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCa                                              <  1:1495443/147‑1 (MQ=255)
                                                                                                 gAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCa                                              <  1:476253/148‑1 (MQ=255)
                                                                                                    gTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACg                                           <  2:3376465/148‑1 (MQ=255)
                                                                                                     ttGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGa                                          >  1:297453/1‑148 (MQ=255)
                                                                                                     ttGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGa                                          <  1:2468090/148‑1 (MQ=255)
                                                                                                      tGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGaa                                         <  1:3085128/148‑1 (MQ=255)
                                                                                                       gcgcATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGaaa                                        <  1:924430/148‑1 (MQ=255)
                                                                                                        cgcATATTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAAc                                       >  2:2387114/1‑148 (MQ=255)
                                                                                                           atatTATCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGc                                    >  2:180460/1‑148 (MQ=255)
                                                                                                            tattatCATCGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGcc                                   <  1:288557/148‑1 (MQ=255)
                                                                                                                atcatcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTAt                               >  1:1952419/1‑148 (MQ=255)
                                                                                                                atcatcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTAt                               <  1:931505/148‑1 (MQ=255)
                                                                                                                 tcatcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATc                              >  2:1618307/1‑148 (MQ=255)
                                                                                                                  catcGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATcc                             <  2:385141/148‑1 (MQ=255)
                                                                                                                     cGACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCg                          >  2:1687883/1‑148 (MQ=255)
                                                                                                                      gACGCAACAAACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGt                         >  1:1441518/1‑148 (MQ=255)
                                                                                                                           aacaaACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGc                    <  2:233707/148‑1 (MQ=255)
                                                                                                                             caaACGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCat                  <  1:986265/148‑1 (MQ=255)
                                                                                                                              aaaCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCata                 <  1:698021/148‑1 (MQ=255)
                                                                                                                               aaCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatat                <  1:2467086/148‑1 (MQ=255)
                                                                                                                                aCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatata               <  2:311320/148‑1 (MQ=255)
                                                                                                                                aCGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatata               <  2:313264/148‑1 (MQ=255)
                                                                                                                                 cGAACCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCatatat              >  1:1559073/1‑148 (MQ=255)
                                                                                                                                   aaCCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAg            <  1:3348904/148‑1 (MQ=255)
                                                                                                                                   aaCCCAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAg            >  2:2205279/1‑148 (MQ=255)
                                                                                                                                     cccAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTg          >  2:2771287/1‑148 (MQ=255)
                                                                                                                                     cccAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTg          <  1:2472603/148‑1 (MQ=255)
                                                                                                                                     cccAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTg          <  2:2614499/148‑1 (MQ=255)
                                                                                                                                     cccAGCCAGGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTg          <  2:211202/148‑1 (MQ=255)
                                                                                                                                     cccAGCCAGGTTATTTCTGAAAGTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTg          <  2:211162/148‑1 (MQ=255)
                                                                                                                                      ccagccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGa         <  2:140351/148‑1 (MQ=255)
                                                                                                                                       cagccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGAc        >  2:2654347/1‑148 (MQ=255)
                                                                                                                                        agccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACg       <  1:3185640/148‑1 (MQ=255)
                                                                                                                                         gccagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGa      <  1:808429/148‑1 (MQ=255)
                                                                                                                                           cagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAtt    >  1:2705032/1‑148 (MQ=255)
                                                                                                                                           cagGTTATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGAtt    >  1:306258/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTGATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGA  >  CP009273/402196‑402484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑