Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 432,762 G→T 100% P277Q (CCG→CAG)  yajO ← 2‑carboxybenzaldehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273432,7620GT97.3% 581.8 / 6.1 188P277Q (CCG→CAG) yajO2‑carboxybenzaldehyde reductase
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base T (111/72);  total (113/75)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 188 total aligned reads displayed.

TATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTC  >  CP009273/432618‑432906
                                                                                                                                                |                                                                                                                                                
      aaTCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTca                                                                                                                                         >  2:493324/1‑148 (MQ=255)
      aaTCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTca                                                                                                                                         >  1:2677308/1‑148 (MQ=255)
        tCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTcaac                                                                                                                                       >  1:1418538/1‑148 (MQ=255)
           tACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCaacaac                                                                                                                                    >  2:1487245/1‑148 (MQ=255)
             cGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCa                                                                                                                                  <  2:2047197/148‑1 (MQ=255)
             cGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCa                                                                                                                                  <  2:2047212/148‑1 (MQ=255)
             cGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCa                                                                                                                                  <  2:2620748/148‑1 (MQ=255)
                   gATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAg                                                                                                                            >  1:1730082/1‑148 (MQ=255)
                   gATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTATTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAAACATGcccg                                                                                                                            >  1:1437051/1‑146 (MQ=255)
                              taCGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCACTGCCCTGTTTACTCAACAACCAGGCCCGCGCAACTtgtg                                                                                                                 >  2:701382/1‑148 (MQ=255)
                               aCGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCCAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGc                                                                                                                >  2:1075804/1‑148 (MQ=255)
                               aCGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGc                                                                                                                >  1:2032817/1‑148 (MQ=255)
                                  gCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCg                                                                                                             <  1:677619/148‑1 (MQ=255)
                                   cGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTACGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCACCTTGTGCTCgt                                                                                                            >  2:216203/1‑148 (MQ=255)
                                     tttCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCgtgt                                                                                                          >  1:1293827/1‑148 (MQ=255)
                                         cAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCCACTTGTGCTCGTGTCGcc                                                                                                      >  2:2386322/1‑148 (MQ=255)
                                          aGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGccc                                                                                                     <  1:2805627/148‑1 (MQ=255)
                                            ttCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGccccc                                                                                                   >  2:235670/1‑148 (MQ=255)
                                            ttCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGccccc                                                                                                   >  2:390858/1‑148 (MQ=255)
                                                 cAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttc                                                                                              >  1:2427444/1‑148 (MQ=255)
                                                   aTCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt                                                                                            >  2:1149760/1‑148 (MQ=255)
                                                   aTCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt                                                                                            >  2:2118907/1‑148 (MQ=255)
                                                   aTCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt                                                                                            >  2:2180515/1‑148 (MQ=255)
                                                    tCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAA‑CAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCa                                                                                          <  2:1109382/148‑1 (MQ=255)
                                                        ttCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTg                                                                                       <  2:2731975/148‑1 (MQ=255)
                                                          ccGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGAc                                                                                     >  2:3194468/1‑148 (MQ=255)
                                                           cGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACg                                                                                    <  1:3238915/148‑1 (MQ=255)
                                                           cGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACg                                                                                    >  1:315570/1‑148 (MQ=255)
                                                             gCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGcc                                                                                  <  1:3322522/148‑1 (MQ=255)
                                                                tCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGt                                                                               >  2:3173335/1‑148 (MQ=255)
                                                                tCAAAGTGATATCCACCGCGTTAAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCAGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGt                                                                               <  1:3011335/148‑1 (MQ=255)
                                                                    aGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAc                                                                           >  2:3072098/1‑148 (MQ=255)
                                                                    aGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAc                                                                           >  1:1014961/1‑148 (MQ=255)
                                                                    aGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAc                                                                           >  2:3072019/1‑148 (MQ=255)
                                                                     gTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAcc                                                                          >  1:2930158/1‑148 (MQ=255)
                                                                      tGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCg                                                                         >  1:2825810/1‑148 (MQ=255)
                                                                      tGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCg                                                                         >  1:1023034/1‑148 (MQ=255)
                                                                      tGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGGTAACCg                                                                         >  2:2923136/1‑148 (MQ=255)
                                                                        atatCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGct                                                                       <  1:734017/148‑1 (MQ=255)
                                                                            ccACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGc                                                                   >  1:587416/1‑148 (MQ=255)
                                                                            ccACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGc                                                                   >  1:607188/1‑148 (MQ=255)
                                                                            ccACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCGTCACTGACGCCTGTTAACCGCTCTGc                                                                   >  2:3471962/1‑148 (MQ=255)
                                                                                cgcgTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATc                                                               >  1:3449328/1‑148 (MQ=255)
                                                                                  cgTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTg                                                             >  2:3475474/1‑148 (MQ=255)
                                                                                  cgTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTg                                                             <  1:2377757/148‑1 (MQ=255)
                                                                                    ttCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTgcg                                                           <  2:1705590/148‑1 (MQ=255)
                                                                                     tCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTgcgc                                                          >  1:2553387/1‑148 (MQ=255)
                                                                                     tCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTgcgc                                                          >  1:2747294/1‑148 (MQ=255)
                                                                                     tCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTgcgc                                                          >  1:2748220/1‑148 (MQ=255)
                                                                                       aaTAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGt                                                        <  1:482747/148‑1 (MQ=255)
                                                                                         tAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa                                                      >  2:1940184/1‑148 (MQ=255)
                                                                                          aGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATTATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAt                                                     >  2:1825052/1‑148 (MQ=255)
                                                                                          aGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCAt                                                     <  2:936195/148‑1 (MQ=255)
                                                                                              catcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTc                                                 >  1:2571212/1‑148 (MQ=255)
                                                                                              catcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTc                                                 <  2:2005545/148‑1 (MQ=255)
                                                                                              catcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTc                                                 <  2:1302/148‑1 (MQ=255)
                                                                                               atcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCa                                                >  1:2808115/1‑148 (MQ=255)
                                                                                               atcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCa                                                <  2:1588770/148‑1 (MQ=255)
                                                                                               atcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCa                                                <  2:2328558/148‑1 (MQ=255)
                                                                                                tcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCAt                                               >  1:112471/1‑148 (MQ=255)
                                                                                                tcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCAt                                               >  1:367812/1‑148 (MQ=255)
                                                                                                tcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCAt                                               >  1:2808390/1‑148 (MQ=255)
                                                                                                tcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCAt                                               >  1:2974804/1‑148 (MQ=255)
                                                                                                tcaAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATATTCAt                                               >  1:367601/1‑148 (MQ=255)
                                                                                                 caAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATc                                              >  1:842457/1‑148 (MQ=255)
                                                                                                    gCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGct                                           >  2:2415423/1‑148 (MQ=255)
                                                                                                    gCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGct                                           >  2:182396/1‑148 (MQ=255)
                                                                                                    gCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGct                                           <  2:2762119/148‑1 (MQ=255)
                                                                                                    gCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGct                                           <  1:1893107/148‑1 (MQ=255)
                                                                                                      tGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGcttt                                         <  1:3185631/148‑1 (MQ=255)
                                                                                                      tGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGcttt                                         <  1:3481835/148‑1 (MQ=255)
                                                                                                       gTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttc                                        >  1:779656/1‑148 (MQ=255)
                                                                                                        ttcttcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttct                                       >  1:3002486/1‑148 (MQ=255)
                                                                                                        ttcttcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttct                                       >  1:1204840/1‑148 (MQ=255)
                                                                                                          cttcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGctttcttt                                     <  2:1657799/148‑1 (MQ=255)
                                                                                                             cgcgcgAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTtata                                  <  1:1543064/148‑1 (MQ=255)
                                                                                                              gcgcgAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATag                                 >  2:105916/1‑148 (MQ=255)
                                                                                                                 cgAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAgaga                              <  1:2226412/148‑1 (MQ=255)
                                                                                                                 cgAAGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAgaga                              <  1:1726255/148‑1 (MQ=255)
                                                                                                                    aGTTCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAttt                           <  2:3042784/148‑1 (MQ=255)
                                                                                                                      ttCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAttttt                         <  2:1293827/148‑1 (MQ=255)
                                                                                                                      ttCCGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGAttttt                         <  2:1198485/148‑1 (MQ=255)
                                                                                                                        ccGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTcc                       >  2:449721/1‑148 (MQ=255)
                                                                                                                         cGATAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTccc                      <  2:1437051/148‑1 (MQ=255)
                                                                                                                           aTAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCa                    >  1:882406/1‑148 (MQ=255)
                                                                                                                            tAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCAc                   >  1:400736/1‑148 (MQ=255)
                                                                                                                            tAATCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCAc                   >  1:2120995/1‑148 (MQ=255)
                                                                                                                              aTCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCt                 <  1:253288/148‑1 (MQ=255)
                                                                                                                               tCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtc                <  2:792799/148‑1 (MQ=255)
                                                                                                                               tCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtc                >  1:928176/1‑148 (MQ=255)
                                                                                                                               tCGGTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtc                <  2:1730082/148‑1 (MQ=255)
                                                                                                                                 ggTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtcat              <  2:887001/148‑1 (MQ=255)
                                                                                                                                  gTGCGGCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCtcatc             >  2:732291/1‑148 (MQ=255)
                                                                                                                                      ggCAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCTCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGac         <  1:2292660/148‑1 (MQ=255)
                                                                                                                                       gCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGaca        >  2:3333337/1‑148 (MQ=255)
                                                                                                                                        cAATGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGacac       >  2:1239170/1‑148 (MQ=255)
                                                                                                                                         aaTGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACAcc      <  1:571968/148‑1 (MQ=255)
                                                                                                                                          aTGCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCa     >  1:1735888/1‑148 (MQ=255)
                                                                                                                                            gCCCTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGt   <  2:855547/148‑1 (MQ=255)
                                                                                                                                             cccTGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTATTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCACATCAGACACCAGTc  >  1:962384/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTC  >  CP009273/432618‑432906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑