Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 49,840 G→T 100% A6A (GCG→GCT)  folA → dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927349,8400GT100.0% 687.1 / NA 205A6A (GCG→GCT) folAdihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (103/102);  total (103/102)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

AGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTG  >  CP009273/49696‑49984
                                                                                                                                                 |                                                                                                                                                
 gTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGt                                                                                                                                              <  1:1375157/148‑1 (MQ=255)
 gTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGt                                                                                                                                              <  1:3122770/148‑1 (MQ=255)
  tCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGtt                                                                                                                                             >  2:3381562/1‑148 (MQ=255)
    cAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAg                                                                                                                                           <  2:809695/148‑1 (MQ=255)
    cAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAg                                                                                                                                           <  2:187484/148‑1 (MQ=255)
      gggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCg                                                                                                                                         >  1:2581266/1‑148 (MQ=255)
      gggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCg                                                                                                                                         >  2:634468/1‑148 (MQ=255)
       ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCgg                                                                                                                                        >  1:2951004/1‑148 (MQ=255)
       ggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCgg                                                                                                                                        >  1:359294/1‑148 (MQ=255)
           agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGa                                                                                                                                    <  2:444976/148‑1 (MQ=255)
                gTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATcgcg                                                                                                                               <  2:2455397/148‑1 (MQ=255)
                 tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGt                                                                                                                              <  2:1436715/148‑1 (MQ=255)
                 tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGt                                                                                                                              >  2:2204525/1‑148 (MQ=255)
                 tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACAACATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGt                                                                                                                              <  2:1430405/148‑1 (MQ=255)
                   gACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTa                                                                                                                            <  1:3397847/148‑1 (MQ=255)
                    aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTAt                                                                                                                           >  2:1275152/1‑148 (MQ=255)
                    aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTAt                                                                                                                           <  1:1118984/148‑1 (MQ=255)
                    aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTAt                                                                                                                           >  1:1951182/1‑148 (MQ=255)
                     cTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATc                                                                                                                          <  1:1402616/148‑1 (MQ=255)
                      tCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCg                                                                                                                         <  2:2137654/148‑1 (MQ=255)
                           agcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATg                                                                                                                    <  2:3379627/148‑1 (MQ=255)
                            gcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATgg                                                                                                                   >  2:2755201/1‑148 (MQ=255)
                               gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGaaa                                                                                                                <  1:2222689/148‑1 (MQ=255)
                               gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGaaa                                                                                                                <  1:863776/148‑1 (MQ=255)
                               gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGaaa                                                                                                                <  1:3378381/148‑1 (MQ=255)
                               gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGaaa                                                                                                                >  1:1330987/1‑148 (MQ=255)
                                aaTATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGaaaa                                                                                                               >  2:1621907/1‑148 (MQ=255)
                                  tataAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACg                                                                                                             >  1:1412386/1‑148 (MQ=255)
                                   ataAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGc                                                                                                            <  2:2342059/148‑1 (MQ=255)
                                          tttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCg                                                                                                     >  1:1205479/1‑148 (MQ=255)
                                          tttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCg                                                                                                     <  1:228857/148‑1 (MQ=255)
                                            tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGt                                                                                                    <  2:409888/148‑1 (MQ=255)
                                               tccaCATCATCCCCGCACCCGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGCTCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGac                                                                                                <  1:1775455/145‑2 (MQ=255)
                                                cAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAc                                                                                               >  2:593563/1‑148 (MQ=255)
                                                   catcatCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctg                                                                                            >  1:3241764/1‑148 (MQ=255)
                                                   catcatCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctg                                                                                            >  2:48405/1‑148 (MQ=255)
                                                    atcatcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgc                                                                                           >  1:1754416/1‑148 (MQ=255)
                                                     tcatcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcc                                                                                          >  1:162517/1‑148 (MQ=255)
                                                       atcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcctg                                                                                        >  1:1365098/1‑148 (MQ=255)
                                                       atcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcctg                                                                                        >  2:1669660/1‑148 (MQ=255)
                                                       atcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcctg                                                                                        >  2:1213791/1‑148 (MQ=255)
                                                         ccTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCtgcctgcc                                                                                      <  1:1293928/148‑1 (MQ=255)
                                                           tCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGa                                                                                    >  2:823543/1‑148 (MQ=255)
                                                           tCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGa                                                                                    >  1:688264/1‑148 (MQ=255)
                                                           tCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGa                                                                                    >  2:1261984/1‑148 (MQ=255)
                                                              cACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGACCCTGCCTGCCGAtct                                                                                 >  2:1858935/1‑148 (MQ=255)
                                                               aCCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTGTCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAtctc                                                                                >  1:2270263/1‑148 (MQ=255)
                                                                ccAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCg                                                                               >  1:2776548/1‑148 (MQ=255)
                                                                    tCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTg                                                                           >  1:3288887/1‑148 (MQ=255)
                                                                      gacgacGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGt                                                                         <  1:3179782/148‑1 (MQ=255)
                                                                          acgGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa                                                                     >  2:1019336/1‑148 (MQ=255)
                                                                             gTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACg                                                                  >  1:3514802/1‑148 (MQ=255)
                                                                                   gCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAacac                                                            <  2:359294/148‑1 (MQ=255)
                                                                                     tttACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt                                                          <  1:1235898/148‑1 (MQ=255)
                                                                                       ttcGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTGATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTta                                                        <  2:2911464/146‑1 (MQ=255)
                                                                                       tACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTta                                                        <  1:1118079/148‑1 (MQ=255)
                                                                                        aCGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaa                                                       <  1:1033006/148‑1 (MQ=255)
                                                                                         cGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaa                                                      <  1:430020/148‑1 (MQ=255)
                                                                                            ataGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataa                                                   <  2:2992161/148‑1 (MQ=255)
                                                                                            ataGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataa                                                   >  2:2160148/1‑148 (MQ=255)
                                                                                             taGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataaa                                                  <  2:3443562/148‑1 (MQ=255)
                                                                                             taGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataaa                                                  <  1:2755201/148‑1 (MQ=255)
                                                                                              aGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAc                                                 >  1:3333835/1‑148 (MQ=255)
                                                                                              aGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAc                                                 >  1:2136040/1‑148 (MQ=255)
                                                                                               gTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAcc                                                <  2:1412386/148‑1 (MQ=255)
                                                                                                 ggCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCg                                              <  1:1923811/148‑1 (MQ=255)
                                                                                                 ggCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCg                                              <  2:2834356/148‑1 (MQ=255)
                                                                                                   cGACAA‑TTTTTTTTATCGGGGAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTg                                            <  2:2796942/148‑1 (MQ=255)
                                                                                                   cGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTg                                            <  1:2970250/148‑1 (MQ=255)
                                                                                                   cGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCAGTg                                            <  1:593563/148‑1 (MQ=255)
                                                                                                    gACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGa                                           <  1:2083711/148‑1 (MQ=255)
                                                                                                    gACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGa                                           <  2:1331758/148‑1 (MQ=255)
                                                                                                      cAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGAtt                                         >  2:3117985/1‑148 (MQ=255)
                                                                                                      cAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGAtt                                         >  1:1531702/1‑148 (MQ=255)
                                                                                                       aa‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                        >  2:2620331/1‑148 (MQ=255)
                                                                                                        a‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTAt                                       >  2:146106/1‑148 (MQ=255)
                                                                                                        a‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTAt                                       <  1:1360260/148‑1 (MQ=255)
                                                                                                           tttttttATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                     <  2:436748/148‑1 (MQ=255)
                                                                                                                    cGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATa                            >  1:136416/1‑148 (MQ=255)
                                                                                                                      ggAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATAcc                          <  2:3288854/148‑1 (MQ=255)
                                                                                                                          aTCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTggg                      >  2:290450/1‑148 (MQ=255)
                                                                                                                               aaTGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGGTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca                 >  2:2056670/1‑148 (MQ=255)
                                                                                                                                 tGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaat               >  2:2670135/1‑148 (MQ=255)
                                                                                                                                 tGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaat               <  2:3419829/148‑1 (MQ=255)
                                                                                                                                 tGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaat               <  2:196961/148‑1 (MQ=255)
                                                                                                                                  gATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc              >  1:2395043/1‑148 (MQ=255)
                                                                                                                                   aTCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCg             >  1:1146812/1‑148 (MQ=255)
                                                                                                                                     cAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgt           <  2:1297845/148‑1 (MQ=255)
                                                                                                                                     cAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgt           >  2:1183079/1‑148 (MQ=255)
                                                                                                                                     cAGTCTGATTGCTGCGTTAGCGGTACCTCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCAGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTCAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgt           <  1:2597571/148‑1 (MQ=255)
                                                                                                                                        tCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgt        >  2:1587700/1‑148 (MQ=255)
                                                                                                                                        tCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCAAAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgt        >  2:2041665/1‑148 (MQ=255)
                                                                                                                                         cTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgtc       >  2:1794070/1‑148 (MQ=255)
                                                                                                                                           gATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg     <  2:3333835/148‑1 (MQ=255)
                                                                                                                                           gATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg     <  2:3499277/148‑1 (MQ=255)
                                                                                                                                           gATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg     <  2:1531702/148‑1 (MQ=255)
                                                                                                                                            aTTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt    >  2:1814903/1‑148 (MQ=255)
                                                                                                                                             ttGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAATTAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGtt   >  2:1320608/1‑148 (MQ=255)
                                                                                                                                             ttGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGACGTCCGtt   >  2:2918813/1‑148 (MQ=255)
                                                                                                                                              tgctgcGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTg  >  1:1211323/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTG  >  CP009273/49696‑49984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑