Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 446,369 T→G 100% Q233P (CAG→CCG)  cyoA ← cytochrome o ubiquinol oxidase subunit II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273446,3690TG98.8% 933.9 / ‑2.8 230Q233P (CAG→CCG) cyoAcytochrome o ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (123/104);  total (125/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 231 total aligned reads displayed.

AAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGG  >  CP009273/446224‑446513
                                                                                                                                                 |                                                                                                                                                
     tGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGaa                                                                                                                                           >  2:815022/1‑148 (MQ=255)
     tGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGaa                                                                                                                                           <  2:1025856/148‑1 (MQ=255)
     tGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGaa                                                                                                                                           >  1:1523769/1‑148 (MQ=255)
     tGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGaa                                                                                                                                           >  1:1752769/1‑148 (MQ=255)
       ttAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATg                                                                                                                                         <  1:1988399/148‑1 (MQ=255)
          aTTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcgg                                                                                                                                      <  1:1628681/148‑1 (MQ=255)
           ttACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcggc                                                                                                                                     >  1:898736/1‑148 (MQ=255)
            tACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcggcg                                                                                                                                    >  1:1109895/1‑148 (MQ=255)
            tACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATgcggcg                                                                                                                                    >  2:1326189/1‑148 (MQ=255)
              cATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGcgcg                                                                                                                                  <  2:327834/148‑1 (MQ=255)
               aTCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGa                                                                                                                                 >  2:1999100/1‑148 (MQ=255)
                tCGGCAAACAAGTCTGGTGTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGAt                                                                                                                                <  2:1824380/148‑1 (MQ=255)
                 cGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATc                                                                                                                               >  2:909338/1‑148 (MQ=255)
                 cGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATc                                                                                                                               <  1:2759242/148‑1 (MQ=255)
                 cGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATc                                                                                                                               >  2:2624493/1‑148 (MQ=255)
                  ggCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATcc                                                                                                                              <  1:855568/148‑1 (MQ=255)
                    cAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCgg                                                                                                                            >  2:488420/1‑148 (MQ=255)
                      aaCAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTCTTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGgtg                                                                                                                          >  2:1864514/1‑148 (MQ=255)
                       aCAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGgtgt                                                                                                                         <  1:3092855/148‑1 (MQ=255)
                       aCAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGgtgt                                                                                                                         <  1:3104394/148‑1 (MQ=255)
                           gTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCa                                                                                                                     <  2:2233860/148‑1 (MQ=255)
                           gTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCa                                                                                                                     <  1:349854/148‑1 (MQ=255)
                           gTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCa                                                                                                                     <  1:1015194/148‑1 (MQ=255)
                             cTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAAt                                                                                                                   <  1:2259958/148‑1 (MQ=255)
                             cTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAAt                                                                                                                   <  1:2260016/148‑1 (MQ=255)
                             cTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAAt                                                                                                                   >  2:2746901/1‑148 (MQ=255)
                               ggTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAg                                                                                                                 <  1:2185905/148‑1 (MQ=255)
                               ggTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAg                                                                                                                 <  2:1514804/148‑1 (MQ=255)
                                gTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGc                                                                                                                <  1:2465881/148‑1 (MQ=255)
                                gTTTCACGTTGGAGAAATATTCCAACTGGTTGTATTCGCTAGGCGCGGCCAGTTTATCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGAGCGATCCGGTGTTGCAATAGc                                                                                                                >  2:170970/1‑148 (MQ=255)
                                         tGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACtt                                                                                                       <  1:2119742/148‑1 (MQ=255)
                                         tGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACtt                                                                                                       >  1:1073460/1‑148 (MQ=255)
                                         tGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACtt                                                                                                       <  1:53807/148‑1 (MQ=255)
                                          ggAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTc                                                                                                      <  1:3376678/148‑1 (MQ=255)
                                                  atTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTga                                                                                              >  1:592404/1‑148 (MQ=255)
                                                    tCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTgaga                                                                                            >  2:233649/1‑148 (MQ=255)
                                                    tCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTgaga                                                                                            >  1:2037911/1‑148 (MQ=255)
                                                    tCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTgaga                                                                                            >  1:1165314/1‑148 (MQ=255)
                                                      cACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAg                                                                                          >  1:2309375/1‑148 (MQ=255)
                                                      cACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGAGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAg                                                                                          <  1:1549773/148‑1 (MQ=255)
                                                      cACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGAGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAg                                                                                          <  1:3291676/148‑1 (MQ=255)
                                                       aCCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGc                                                                                         <  1:1099978/148‑1 (MQ=255)
                                                        ccTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGcc                                                                                        >  1:1754007/1‑148 (MQ=255)
                                                             ttGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGc                                                                                   >  1:2725923/1‑148 (MQ=255)
                                                             ttGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGc                                                                                   >  1:512302/1‑148 (MQ=255)
                                                              tGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGcc                                                                                  >  1:1021756/1‑148 (MQ=255)
                                                                tATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGc                                                                                <  2:1538575/148‑1 (MQ=255)
                                                                  ttCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTg                                                                              <  2:2635936/148‑1 (MQ=255)
                                                                  ttCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTg                                                                              >  1:928541/1‑148 (MQ=255)
                                                                     gCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAg                                                                           <  2:2550015/148‑1 (MQ=255)
                                                                     gCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAg                                                                           <  2:646609/148‑1 (MQ=255)
                                                                      cTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGc                                                                          >  1:2931721/1‑148 (MQ=255)
                                                                          gcgcGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGc                                                                      >  2:1869902/1‑148 (MQ=255)
                                                                             cgGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGga                                                                   <  2:739088/148‑1 (MQ=255)
                                                                             cgGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGga                                                                   <  2:1465857/148‑1 (MQ=255)
                                                                              ggCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGgag                                                                  <  2:1057125/148‑1 (MQ=255)
                                                                               gCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGgaga                                                                 >  2:2128500/1‑148 (MQ=255)
                                                                               gCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGgaga                                                                 <  1:1474616/148‑1 (MQ=255)
                                                                                ccAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAt                                                                <  2:3506495/148‑1 (MQ=255)
                                                                                 cAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATa                                                               <  1:214652/148‑1 (MQ=255)
                                                                                 cAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATa                                                               <  2:1551986/148‑1 (MQ=255)
                                                                                  aGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATAc                                                              <  2:2667480/148‑1 (MQ=255)
                                                                                    tttttCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCg                                                            >  2:1839760/1‑148 (MQ=255)
                                                                                     ttttCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGt                                                           >  1:2377803/1‑148 (MQ=255)
                                                                                       ttCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCa                                                         >  1:655086/1‑148 (MQ=255)
                                                                                       ttCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCa                                                         >  1:1317774/1‑148 (MQ=255)
                                                                                          gAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATaa                                                      <  1:1948446/148‑1 (MQ=255)
                                                                                            aCGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGt                                                    <  1:2723475/148‑1 (MQ=255)
                                                                                            aCGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGt                                                    <  1:3327223/148‑1 (MQ=255)
                                                                                              gCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGc                                                  >  1:1882989/1‑148 (MQ=255)
                                                                                               cAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGcc                                                 >  2:172392/1‑148 (MQ=255)
                                                                                                  ccATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCggg                                              >  2:172777/1‑148 (MQ=255)
                                                                                                   cATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGt                                             <  1:1837781/148‑1 (MQ=255)
                                                                                                    aTGTCAGACATGGTGTTCGGCGACTGCTTCGCTATTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGtt                                            <  2:2436650/148‑1 (MQ=255)
                                                                                                     tGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGGTc                                           <  2:1948871/148‑1 (MQ=255)
                                                                                                      gTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCg                                          <  2:521897/148‑1 (MQ=255)
                                                                                                      gTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCg                                          >  2:419629/1‑148 (MQ=255)
                                                                                                       tCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGt                                         >  2:3399882/1‑148 (MQ=255)
                                                                                                       tCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGt                                         <  1:1758290/148‑1 (MQ=255)
                                                                                                       tCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGt                                         <  1:799471/148‑1 (MQ=255)
                                                                                                          gACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTgg                                      >  2:292572/1‑148 (MQ=255)
                                                                                                            cATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCg                                    >  2:2160852/1‑148 (MQ=255)
                                                                                                             aTGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGa                                   <  1:2036691/148‑1 (MQ=255)
                                                                                                               ggTGTTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATc                                 >  2:3340501/1‑148 (MQ=255)
                                                                                                                gtgtTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCa                                >  2:1569357/1‑148 (MQ=255)
                                                                                                                gtgtTCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCa                                >  1:1665936/1‑148 (MQ=255)
                                                                                                                   ttCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGAt                             >  1:2083770/1‑148 (MQ=255)
                                                                                                                    tCGGCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATg                            >  1:818772/1‑148 (MQ=255)
                                                                                                                      ggCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCCATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCGGTAGCTGGCGGAGATAACGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCa                          >  1:2447466/1‑148 (MQ=255)
                                                                                                                      ggCGACTGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCa                          >  1:2339030/1‑148 (MQ=255)
                                                                                                                            tGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAg                    >  2:554895/1‑148 (MQ=255)
                                                                                                                            tGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAg                    <  1:2288883/148‑1 (MQ=255)
                                                                                                                            tGCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAg                    >  2:3475805/1‑148 (MQ=255)
                                                                                                                             gCTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGt                   >  1:247898/1‑148 (MQ=255)
                                                                                                                              cTTCGCTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTc                  <  1:1340317/148‑1 (MQ=255)
                                                                                                                                   cTTTTGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCAt             <  1:2986486/148‑1 (MQ=255)
                                                                                                                                    ttttGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATa            >  2:2773782/1‑148 (MQ=255)
                                                                                                                                     tttGCGACCCACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATAc           <  2:2050202/148‑1 (MQ=255)
                                                                                                                                            cccACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCAt    <  1:2573417/148‑1 (MQ=255)
                                                                                                                                              cACGGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATgg  >  1:578318/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGG  >  CP009273/446224‑446513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑