Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,290 G→A 100% intergenic (+45/‑299) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,2900GA100.0% 671.1 / ‑6.7 204intergenic (+45/‑299)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (90/113);  minor base C (0/1);  total (90/114)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 208 total aligned reads displayed.

TGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGC  >  CP009273/450146‑450434
                                                                                                                                                |                                                                                                                                                    
tGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtt                                                                                                                                                   >  1:2574505/1‑148 (MQ=255)
 gcgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAttt                                                                                                                                                  >  1:2522146/1‑148 (MQ=255)
  cgcTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAAtttt                                                                                                                                                 <  2:2639443/148‑1 (MQ=255)
      ggCTCTGCATACTTACACTATTAATGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                             <  1:2370094/148‑1 (MQ=255)
      ggCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                             <  1:2370039/148‑1 (MQ=255)
      ggCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGa                                                                                                                                             <  1:2930684/148‑1 (MQ=255)
        ctctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaa                                                                                                                                           <  1:151326/148‑1 (MQ=255)
          ctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                         <  1:2089271/148‑1 (MQ=255)
          ctGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGaaaaa                                                                                                                                         <  1:3141996/148‑1 (MQ=255)
              aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGc                                                                                                                                     <  1:3333304/148‑1 (MQ=255)
              aTACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGc                                                                                                                                     <  2:2532078/148‑1 (MQ=255)
               tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGANGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                                    >  1:1785293/1‑148 (MQ=255)
               tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                                    <  1:2782474/148‑1 (MQ=255)
               tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                                    >  1:2406339/1‑148 (MQ=255)
               tACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGcc                                                                                                                                    <  2:741562/148‑1 (MQ=255)
                   tACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCg                                                                                                                                >  1:325857/1‑148 (MQ=255)
                   tACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCg                                                                                                                                >  1:1141140/1‑148 (MQ=255)
                      acTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGc                                                                                                                             >  2:1577598/1‑148 (MQ=255)
                          ttAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtt                                                                                                                         >  2:751361/1‑148 (MQ=255)
                          ttAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtt                                                                                                                         >  1:3032791/1‑148 (MQ=255)
                          ttAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtt                                                                                                                         <  2:2913176/148‑1 (MQ=255)
                            aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtttt                                                                                                                       <  2:1472983/148‑1 (MQ=255)
                             aGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg                                                                                                                      >  2:2120048/1‑148 (MQ=255)
                             aGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttg                                                                                                                      <  1:1473142/148‑1 (MQ=255)
                              ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttgt                                                                                                                     <  2:2633888/148‑1 (MQ=255)
                               gAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttttt                                                                                                                    >  1:1847820/1‑148 (MQ=255)
                               gAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttgtt                                                                                                                    <  1:390064/148‑1 (MQ=255)
                               gAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttgtt                                                                                                                    >  1:1169512/1‑148 (MQ=255)
                               gAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttgtt                                                                                                                    >  1:1169606/1‑148 (MQ=255)
                                 gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttttgtttt                                                                                                                  <  2:1973847/148‑1 (MQ=255)
                                  tGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgttgttttttt                                                                                                                 >  1:1878167/1‑147 (MQ=255)
                                   gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTtttgttttgt                                                                                                                <  2:510335/148‑1 (MQ=255)
                                     gAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCGgtggtgttttttgt                                                                                                              >  1:757020/1‑144 (MQ=255)
                                       aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCgtgttttttttttttg                                                                                                            >  1:1287810/1‑142 (MQ=255)
                                        gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGa                                                                                                          <  2:295653/148‑1 (MQ=255)
                                               aGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTgcg                                                                                                   <  2:1705991/148‑1 (MQ=255)
                                                   cacCGTCTTGGCCTCTCCTCCCGGTCGTGGAGCCGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCGTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCttt                                                                                               <  2:2287639/148‑1 (MQ=255)
                                                    acCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCtttt                                                                                              <  2:48129/148‑1 (MQ=255)
                                                      cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTg                                                                                            <  2:2182061/148‑1 (MQ=255)
                                                      cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTg                                                                                            <  2:1564082/148‑1 (MQ=255)
                                                       gTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTGTTTTGTGT‑GGTTTTTTCGCttttttg                                                                                           >  1:2594709/1‑146 (MQ=255)
                                                         cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGccc                                                                                         <  2:2901930/148‑1 (MQ=255)
                                                         cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGccc                                                                                         <  2:3147616/148‑1 (MQ=255)
                                                         cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGccc                                                                                         <  2:2236845/148‑1 (MQ=255)
                                                           ttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTCT‑GGATTTTGCGCTTTTTGccccg                                                                                       >  2:1068699/1‑146 (MQ=255)
                                                             gCCTCTCCTCCCTGTCGTGGAGCAGTAAGAATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCATTATGTTGATAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCa                                                                                     <  1:46078/148‑1 (MQ=255)
                                                             gCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCa                                                                                     <  1:864188/148‑1 (MQ=255)
                                                                tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTc                                                                                  <  1:721243/148‑1 (MQ=255)
                                                                tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTGT‑GGATTTTGCGCTTTTTGCCCAGCATTc                                                                                  >  2:1862970/1‑148 (MQ=255)
                                                                tctcCTCCCTGTCGTGGAGCAGGAAGAATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGACGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGGCCAGcgatc                                                                                  >  2:1862338/1‑144 (MQ=255)
                                                                 ctcctcCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                 <  1:1115497/148‑1 (MQ=255)
                                                                   cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑a                                                                              <  1:2310263/148‑1 (MQ=255)
                                                                   cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑a                                                                              <  2:178476/148‑1 (MQ=255)
                                                                   cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑a                                                                              <  2:294599/148‑1 (MQ=255)
                                                                    ctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGGTTGGTTTTTTGT‑GGGTTTTTGGGTTTTTTTGCCGCAATCAG‑Ac                                                                             >  1:427178/1‑148 (MQ=255)
                                                                        cTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGT‑GGTTTTTTGGCTTTTTGGCCAGCAATAAG‑AC‑G‑aaa                                                                       >  1:2623743/1‑148 (MQ=255)
                                                                        cTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑aaa                                                                       <  2:3373135/148‑1 (MQ=255)
                                                                         tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑aaaa                                                                      <  2:3510196/148‑1 (MQ=255)
                                                                         tGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑aaaa                                                                      <  1:65117/148‑1 (MQ=255)
                                                                            cGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTg                                                                   <  1:392094/148‑1 (MQ=255)
                                                                            cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTg                                                                   >  2:2558214/1‑148 (MQ=255)
                                                                            cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTGGTTTTTGTGGGGTTTTGGGGTTTTTGCCC‑GCATTAAGTAC‑A‑AAAAtt                                                                    >  1:1308921/1‑148 (MQ=255)
                                                                              tGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGTTTTTGCGCTTTTTGACCACCATTCAG‑AC‑G‑AAAATTGcc                                                                 >  2:1181759/1‑148 (MQ=255)
                                                                                    aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGaa                                                           <  1:1660674/148‑1 (MQ=255)
                                                                                    aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGaa                                                           <  2:1918446/148‑1 (MQ=255)
                                                                                     ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAAt                                                          <  1:559610/148‑1 (MQ=255)
                                                                                      gAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTGTTTTTTTGTGGGTTTTTGGGTTTTTTTCCCCCCA‑TAAG‑TC‑G‑AAAATTTGCCGGGGAtt                                                         >  1:1042658/1‑148 (MQ=255)
                                                                                       aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATtg                                                        <  1:2049146/148‑1 (MQ=255)
                                                                                       aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGGTTTGTGT‑GGATTTTGCGGGTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTTCCCGGGAATtg                                                        >  2:1262561/1‑148 (MQ=255)
                                                                                        aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATtgt                                                       <  1:2921265/148‑1 (MQ=255)
                                                                                          cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTAGGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGa                                                     <  2:3278362/148‑1 (MQ=255)
                                                                                             cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGGTGTTTTGTGT‑GGATTTTGCGCTTTTTGCCCCTCATTCAG‑AC‑G‑AAAATTGCCCGGGCATTGTGtaaa                                                  >  2:1395351/1‑148 (MQ=255)
                                                                                                 tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATa                                              <  1:284346/148‑1 (MQ=255)
                                                                                                    aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTGT‑TGATTTTTCGGTTTTTTCCCAGCATTCAG‑AC‑G‑AAAATTTCCCGGGAATTGTGTAAAAATAcgc                                           >  2:3352672/1‑148 (MQ=255)
                                                                                                    aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGT‑GGAGTTTGGGCTTTTTTGCCAGCAGTCAG‑AC‑G‑AAAAATTCCCGGGAATTGTGaaaaaaaaagc                                           >  2:2430896/1‑143 (MQ=255)
                                                                                                    aaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGGTTTTTGT‑GGTTTTTTGGCTTTTTGGCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGGATTGTGTAAAATTAcgc                                           >  1:3210538/1‑148 (MQ=255)
                                                                                                     aaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGT‑TGCTTTTTGGGGTTTTGGCCAGCCTTCAG‑TCGG‑AAAATTG‑CCGGGGATTTTGTAAAAATAagcg                                          >  1:2031334/1‑148 (MQ=255)
                                                                                                      aCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGa                                         >  2:2038563/1‑148 (MQ=255)
                                                                                                            ttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTGTTTTTTGT‑GGATTTTTCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcgc                                   >  2:2268187/1‑148 (MQ=255)
                                                                                                             tGCAACTGTGGGGGCTTTTCCAGTATTTTGCTAAAATTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcgcg                                  <  1:2226800/148‑1 (MQ=255)
                                                                                                               cAACTGTCGGCGCTTTTCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCa                                <  1:2774300/148‑1 (MQ=255)
                                                                                                                aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCaa                               <  2:2796323/148‑1 (MQ=255)
                                                                                                                 aCTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAATGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAAt                              <  2:2046881/148‑1 (MQ=255)
                                                                                                                  cTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGT‑TGATTTTTGGGTTTTTTGCCACCAATCAG‑AC‑GAAAAATTGCCCGGCCA‑TGTGTAAAAATAAGCGACAgcgcgccaaa                             >  1:2267750/1‑144 (MQ=255)
                                                                                                                  cTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                             <  2:3466515/148‑1 (MQ=255)
                                                                                                                  cTGTCGGCGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                             <  2:1729076/148‑1 (MQ=255)
                                                                                                                    gTCGGCGCATTTCCAGTATGTGGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAc                           <  2:1882110/148‑1 (MQ=255)
                                                                                                                    gTCGGAGCTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCTGGCAGTTTTTTTTTGTCT‑TGATTTTTGGCTTTTTTACCAGCAGTCAG‑TA‑G‑AAAATTTCCCTGGAATTTTGTAAAAATACGCGGAAGCGGGCAaaaaa                           >  2:235643/1‑147 (MQ=255)
                                                                                                                         cgcTTTTCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGT‑GGATTTTGCGCTTTTTGCCCAGCATTAAG‑AC‑G‑AAAAATTCCCGGGAATTGTGAAAAAATAAGCGAAAGCGCGCAATaaaccgac                      >  2:1668422/1‑142 (MQ=255)
                                                                                                                            ttttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCACCAATCAG‑AC‑G‑AAAATTGCCCGGGAAATGTGAAAAAATACGCGAAAGCGCGCAATAAACCTTATCg                   >  2:1140074/1‑148 (MQ=255)
                                                                                                                             tttCCAGTATGTTGATAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                  <  1:1330863/148‑1 (MQ=255)
                                                                                                                             gttCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                  <  2:1985670/147‑1 (MQ=255)
                                                                                                                               tCCAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTTGT‑TGATTTTTCGCTTTTTTCCCAGCAATAAG‑AC‑G‑AAAATTTCCCGGGAATTGTGAAAAAATAAGCGACAGCGCGCACAAACCGGTCTCGaaa                >  2:674740/1‑146 (MQ=255)
                                                                                                                                 cAGTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCa              <  2:1736787/148‑1 (MQ=255)
                                                                                                                                   gTATGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTGT‑GGGTTTTTCGCTTTTTTCCCAGCATTCAC‑AC‑G‑AAAAATTCCCGGGTAATGTTTAAAAAAAAACGACAGCGCGCAAAAAACCTTATAGACACATa            >  1:3491725/1‑148 (MQ=255)
                                                                                                                                     aggTTGCTAAAAATTTTATCAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaa          <  2:2370480/146‑1 (MQ=255)
                                                                                                                                     aTGTTGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaa          <  2:1287810/148‑1 (MQ=255)
                                                                                                                                        ttGCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt       <  2:600746/148‑1 (MQ=255)
                                                                                                                                          gCTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa     <  1:3386142/148‑1 (MQ=255)
                                                                                                                                           cTAAAAATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt    <  1:1991818/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TGCGCTGGCTCTGCATACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCT‑GGATTTTGCGCTTTTTGCCCAGCATTCAG‑AC‑G‑AAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGC  >  CP009273/450146‑450434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑