Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 456,298 T→A 100% L652Q (CTG→CAG)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273456,2980TA100.0% 711.9 / NA 217L652Q (CTG→CAG) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (92/125);  total (92/125)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 217 total aligned reads displayed.

ACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGA  >  CP009273/456153‑456442
                                                                                                                                                 |                                                                                                                                                
 cGGCAGTGGGCGGTGACGTGCTGACCATTGCCACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAggg                                                                                                                                               <  2:2296463/148‑1 (MQ=255)
   gAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGa                                                                                                                                             <  2:1945505/148‑1 (MQ=255)
    aaGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGAt                                                                                                                                            <  2:740009/148‑1 (MQ=255)
      gTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCa                                                                                                                                          <  1:731913/148‑1 (MQ=255)
         ggCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAAcc                                                                                                                                       >  2:1962703/1‑148 (MQ=255)
         ggCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAAcc                                                                                                                                       <  1:2998393/148‑1 (MQ=255)
         ggCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAAcc                                                                                                                                       <  1:1884947/148‑1 (MQ=255)
         ggCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAAcc                                                                                                                                       <  1:1195179/148‑1 (MQ=255)
             gTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGa                                                                                                                                   <  1:411894/148‑1 (MQ=255)
             gTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGa                                                                                                                                   <  1:3444800/148‑1 (MQ=255)
             gTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGa                                                                                                                                   <  2:1792045/148‑1 (MQ=255)
              tGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAt                                                                                                                                  <  2:1323987/148‑1 (MQ=255)
                aCTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGAttt                                                                                                                                >  1:51050/1‑148 (MQ=255)
                    gCTGACCATTGAAAACGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCTAATTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTAc                                                                                                                            >  1:217668/1‑148 (MQ=255)
                          cATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGaaaaa                                                                                                                      >  1:101564/1‑148 (MQ=255)
                            ttGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACg                                                                                                                    >  2:3464647/1‑148 (MQ=255)
                            ttGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACg                                                                                                                    <  1:938361/148‑1 (MQ=255)
                              gAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTg                                                                                                                  >  2:2540462/1‑148 (MQ=255)
                              gAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTg                                                                                                                  <  2:616742/148‑1 (MQ=255)
                                aaCCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGAc                                                                                                                <  1:472344/148‑1 (MQ=255)
                                aaCCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGAc                                                                                                                >  2:1683103/1‑148 (MQ=255)
                                aaCCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGAc                                                                                                                >  2:3420282/1‑148 (MQ=255)
                                 aCCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACa                                                                                                               >  2:3251220/1‑148 (MQ=255)
                                  ccGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAt                                                                                                              <  1:104820/148‑1 (MQ=255)
                                  ccGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAt                                                                                                              <  2:1117843/148‑1 (MQ=255)
                                   cGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtc                                                                                                             <  1:1550023/148‑1 (MQ=255)
                                   cGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtc                                                                                                             <  2:2722063/148‑1 (MQ=255)
                                           ttCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccacgtcca                                                                                                     >  2:1054470/1‑148 (MQ=255)
                                            tCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccacgtccac                                                                                                    >  2:1882503/1‑148 (MQ=255)
                                             ccGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtccacgtccacg                                                                                                   >  2:1870566/1‑148 (MQ=255)
                                                ggTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCAGTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTAc                                                                                                <  2:2459943/148‑1 (MQ=255)
                                                ggTAAAGGCAAACTGACCTATACCGGGTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTAc                                                                                                <  2:2239906/148‑1 (MQ=255)
                                                 gTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTAcc                                                                                               >  2:2585976/1‑148 (MQ=255)
                                                  tAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCg                                                                                              >  2:350266/1‑148 (MQ=255)
                                                      ggCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAg                                                                                          <  1:81082/148‑1 (MQ=255)
                                                      ggCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAg                                                                                          <  2:147264/148‑1 (MQ=255)
                                                       tcAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAgg                                                                                         <  2:2533591/147‑1 (MQ=255)
                                                       gCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAgg                                                                                         <  2:794768/148‑1 (MQ=255)
                                                        cAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGt                                                                                        <  1:2247725/148‑1 (MQ=255)
                                                          aaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGc                                                                                      <  1:3475769/148‑1 (MQ=255)
                                                          aaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGc                                                                                      >  2:2359755/1‑148 (MQ=255)
                                                          aaCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGc                                                                                      >  1:3392254/1‑148 (MQ=255)
                                                           aCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCg                                                                                     <  1:278950/148‑1 (MQ=255)
                                                           aCTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCg                                                                                     >  1:1969899/1‑148 (MQ=255)
                                                                ccTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGcc                                                                                >  1:165754/1‑148 (MQ=255)
                                                                ccTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGcc                                                                                <  2:3309179/148‑1 (MQ=255)
                                                                   ataCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGaa                                                                             >  1:2813107/1‑148 (MQ=255)
                                                                     aCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAg                                                                           >  1:79814/1‑148 (MQ=255)
                                                                       cGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGAt                                                                         >  2:2758784/1‑148 (MQ=255)
                                                                        ggTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCTGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATg                                                                        <  2:1436845/148‑1 (MQ=255)
                                                                          ttCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGt                                                                      >  2:1897974/1‑148 (MQ=255)
                                                                          ttCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGt                                                                      >  1:3323387/1‑148 (MQ=255)
                                                                          ttCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGt                                                                      >  1:3512705/1‑148 (MQ=255)
                                                                              cTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGa                                                                  >  2:2924871/1‑148 (MQ=255)
                                                                                 ggCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTg                                                               <  1:2638256/148‑1 (MQ=255)
                                                                                       gTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTa                                                         <  1:1127494/148‑1 (MQ=255)
                                                                                        tGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTAt                                                        >  2:2413363/1‑148 (MQ=255)
                                                                                         gATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTAtt                                                       <  1:2835926/148‑1 (MQ=255)
                                                                                            gCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCt                                                    <  2:3169478/148‑1 (MQ=255)
                                                                                            gCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCt                                                    >  1:2298736/1‑148 (MQ=255)
                                                                                             cAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTa                                                   <  2:1380024/148‑1 (MQ=255)
                                                                                                gAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTAtgt                                                >  1:212290/1‑148 (MQ=255)
                                                                                                 aGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTAtgtg                                               <  1:628765/148‑1 (MQ=255)
                                                                                                 aGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTAtgtg                                               <  1:1602931/148‑1 (MQ=255)
                                                                                                   tCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCa                                             >  2:969325/1‑148 (MQ=255)
                                                                                                   tCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCa                                             <  2:1248614/148‑1 (MQ=255)
                                                                                                        tCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACcgcg                                        <  2:2245382/148‑1 (MQ=255)
                                                                                                           ggcggcGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTg                                     >  1:3080096/1‑148 (MQ=255)
                                                                                                           ggcggcGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTg                                     <  1:3251220/148‑1 (MQ=255)
                                                                                                            gcggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATNCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTgg                                    <  1:1783300/148‑1 (MQ=255)
                                                                                                            gcggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTgg                                    >  1:2478011/1‑148 (MQ=255)
                                                                                                             cggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGt                                   <  1:2540462/148‑1 (MQ=255)
                                                                                                               gcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGttt                                 <  2:2130825/148‑1 (MQ=255)
                                                                                                               gcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGttt                                 <  2:363066/148‑1 (MQ=255)
                                                                                                               gcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGttt                                 >  1:1440962/1‑148 (MQ=255)
                                                                                                                cgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTc                                >  2:1552481/1‑148 (MQ=255)
                                                                                                                cgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTc                                <  2:2904705/148‑1 (MQ=255)
                                                                                                                cgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTc                                >  2:1229648/1‑148 (MQ=255)
                                                                                                                 gTTAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCt                               <  2:879238/148‑1 (MQ=255)
                                                                                                                   tAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTg                             >  2:2978903/1‑148 (MQ=255)
                                                                                                                   tAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTg                             >  2:3346017/1‑148 (MQ=255)
                                                                                                                   tAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTg                             >  2:975913/1‑148 (MQ=255)
                                                                                                                   tAACGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTg                             >  2:194867/1‑148 (MQ=255)
                                                                                                                    aaCGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGc                            <  1:882635/148‑1 (MQ=255)
                                                                                                                    aaCGGTGGTTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGc                            <  1:934183/148‑1 (MQ=255)
                                                                                                                        gtggtTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTCTGTGCACCGCGCTGGTTTCTTGCCTGa                        >  2:402458/1‑148 (MQ=255)
                                                                                                                           gtTCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCg                     >  1:2984325/1‑148 (MQ=255)
                                                                                                                            ttCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCgg                    <  2:2581308/148‑1 (MQ=255)
                                                                                                                            ttCGTGCGCGTGCGGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCgg                    <  2:3511757/148‑1 (MQ=255)
                                                                                                                                tgcgcgtgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAAc                >  2:1408173/1‑148 (MQ=255)
                                                                                                                                 gcgcgtgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTTCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAAcc               <  2:540562/148‑1 (MQ=255)
                                                                                                                                      tgcgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGtt          <  1:2783723/148‑1 (MQ=255)
                                                                                                                                        cgGAAAAACAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCg        <  1:2388426/148‑1 (MQ=255)
                                                                                                                                           aaaaaCAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGc     >  1:2907869/1‑148 (MQ=255)
                                                                                                                                           aaaaaCAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGc     <  1:1836444/148‑1 (MQ=255)
                                                                                                                                           aaaaaCAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGc     <  1:1625854/148‑1 (MQ=255)
                                                                                                                                             aaaCAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCg   <  2:1270642/148‑1 (MQ=255)
                                                                                                                                             aaaCAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCg   >  2:2369738/1‑148 (MQ=255)
                                                                                                                                             aaaCAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCg   <  2:2612973/148‑1 (MQ=255)
                                                                                                                                              aaCAGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGa  >  1:2682500/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCACGTCCACGTACCGGAAGGTGCGACGCCGAAAGATGGTCCGAGTGCCGGTATTGCTATGTGCACCGCGCTGGTTTCTTGCCTGACCGGTAACCCGGTTCGTGCCGA  >  CP009273/456153‑456442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑