Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 461,499 G→T 100% R424S (CGC→AGC)  ybaE ← putative ABC superfamily transporter periplasmic‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273461,4990GT98.8% 779.3 / 0.2 242R424S (CGC→AGC) ybaEputative ABC superfamily transporter periplasmic‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (126/112);  total (128/114)
Fisher's exact test for biased strand distribution p-value = 6.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 244 total aligned reads displayed.

AGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGA  >  CP009273/461354‑461642
                                                                                                                                                 |                                                                                                                                               
 gagTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTc                                                                                                                                              <  2:1897687/148‑1 (MQ=255)
 gagTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCGTCCGCTGCCAGTGTCGCCTGTAGGCTTTc                                                                                                                                              <  2:2263450/148‑1 (MQ=255)
   gTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTg                                                                                                                                            >  1:822976/1‑148 (MQ=255)
   gTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCGGTAGGCTTTCTg                                                                                                                                            >  1:822986/1‑148 (MQ=255)
    tataTTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCttc                                                                                                                                           >  2:2355224/1‑146 (MQ=255)
      tatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCa                                                                                                                                         >  1:2993432/1‑148 (MQ=255)
      tatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCa                                                                                                                                         >  1:1145435/1‑148 (MQ=255)
      tatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCa                                                                                                                                         >  1:150157/1‑148 (MQ=255)
      tatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCa                                                                                                                                         >  1:150305/1‑148 (MQ=255)
       atTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCAt                                                                                                                                        >  1:2424647/1‑148 (MQ=255)
        ttCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATg                                                                                                                                       >  2:3148630/1‑148 (MQ=255)
        ttCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATg                                                                                                                                       >  2:236540/1‑148 (MQ=255)
         tCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATgg                                                                                                                                      >  2:2881704/1‑148 (MQ=255)
          ccGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGt                                                                                                                                     <  2:2211517/148‑1 (MQ=255)
          ccGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGt                                                                                                                                     <  2:1208166/148‑1 (MQ=255)
              tGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGa                                                                                                                                 <  2:521728/148‑1 (MQ=255)
              tGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGa                                                                                                                                 >  1:1189561/1‑148 (MQ=255)
              tGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGa                                                                                                                                 <  2:1567469/148‑1 (MQ=255)
               gCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTGTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGaa                                                                                                                                <  2:683645/148‑1 (MQ=255)
                    gCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCt                                                                                                                           <  1:3307393/148‑1 (MQ=255)
                     ccAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTa                                                                                                                          >  2:1129168/1‑148 (MQ=255)
                     ccAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTa                                                                                                                          <  2:939451/148‑1 (MQ=255)
                      cAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTAt                                                                                                                         >  1:1111815/1‑148 (MQ=255)
                      cAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTAt                                                                                                                         >  1:2657961/1‑148 (MQ=255)
                        aTTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCg                                                                                                                       >  1:1647511/1‑148 (MQ=255)
                         ttAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCCGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCgg                                                                                                                      >  1:2365393/1‑148 (MQ=255)
                          tAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGt                                                                                                                     <  1:2984686/148‑1 (MQ=255)
                          tAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGt                                                                                                                     >  2:2076781/1‑148 (MQ=255)
                          tAAACTGTCGCCCATCATGAGGTCTGCGTGTGCCTGGAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGt                                                                                                                     <  2:1283504/148‑1 (MQ=255)
                           aaTCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTa                                                                                                                    <  1:336880/148‑1 (MQ=255)
                           aaTCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTa                                                                                                                    <  2:2489504/148‑1 (MQ=255)
                           aaTCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTa                                                                                                                    <  2:1419968/148‑1 (MQ=255)
                            aTCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAg                                                                                                                   <  1:3180303/148‑1 (MQ=255)
                             tCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAgg                                                                                                                  <  1:1058235/148‑1 (MQ=255)
                              cTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGt                                                                                                                 <  1:3474452/148‑1 (MQ=255)
                              cTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGt                                                                                                                 <  2:1397049/148‑1 (MQ=255)
                                gTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGa                                                                                                               >  2:618764/1‑148 (MQ=255)
                                gTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTGTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGa                                                                                                               <  2:3289248/148‑1 (MQ=255)
                                 tCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGAt                                                                                                              <  1:557924/148‑1 (MQ=255)
                                    cccATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATaaa                                                                                                           >  1:3311374/1‑148 (MQ=255)
                                      catcatGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAAcc                                                                                                         >  2:2997077/1‑148 (MQ=255)
                                         catGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGc                                                                                                      >  2:2683484/1‑148 (MQ=255)
                                         catGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCATCCGCAGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGc                                                                                                      >  1:247941/1‑148 (MQ=255)
                                          atGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCg                                                                                                     <  1:701372/148‑1 (MQ=255)
                                                 cTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGt                                                                                              >  1:1397375/1‑148 (MQ=255)
                                                     gtgtGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTc                                                                                          <  2:1853588/148‑1 (MQ=255)
                                                       gtgCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTTGTTTATAAACAACCTTAAAGGTTTTggg                                                                                        >  1:1610543/1‑148 (MQ=255)
                                                       gtgCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCgg                                                                                        <  2:816223/148‑1 (MQ=255)
                                                         gCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTa                                                                                      >  2:2964615/1‑148 (MQ=255)
                                                           cTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGt                                                                                    <  2:3194550/148‑1 (MQ=255)
                                                           cTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATAGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGt                                                                                    >  1:1540729/1‑148 (MQ=255)
                                                            tGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGtt                                                                                   <  1:2126508/148‑1 (MQ=255)
                                                                    cgtgtcgtCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCa                                                                           >  1:2967975/1‑148 (MQ=255)
                                                                      tgtcgtCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATc                                                                         >  2:1741861/1‑148 (MQ=255)
                                                                        tcgtcCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCg                                                                       >  1:2216385/1‑148 (MQ=255)
                                                                             ccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAcc                                                                  >  2:1795794/1‑148 (MQ=255)
                                                                             ccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAcc                                                                  <  1:2258964/148‑1 (MQ=255)
                                                                                gTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGc                                                               <  2:2899216/148‑1 (MQ=255)
                                                                                gTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGc                                                               <  2:150305/148‑1 (MQ=255)
                                                                                 ttttttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGcc                                                              >  2:2778820/1‑148 (MQ=255)
                                                                                  tttttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCa                                                             <  2:1082157/148‑1 (MQ=255)
                                                                                  tttttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCa                                                             <  2:899877/148‑1 (MQ=255)
                                                                                  tttttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCa                                                             >  1:1487539/1‑148 (MQ=255)
                                                                                   ttttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCaa                                                            <  2:977225/148‑1 (MQ=255)
                                                                                       gCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCg                                                        <  1:2773645/148‑1 (MQ=255)
                                                                                          ttATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGaa                                                     >  2:477263/1‑148 (MQ=255)
                                                                                          ttATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGaa                                                     <  2:2621543/148‑1 (MQ=255)
                                                                                            aTGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATa                                                   >  2:673200/1‑148 (MQ=255)
                                                                                            aTGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATa                                                   <  1:3354874/148‑1 (MQ=255)
                                                                                              gAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGt                                                 <  2:342698/148‑1 (MQ=255)
                                                                                               aaaaaTAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTc                                                <  1:2528855/148‑1 (MQ=255)
                                                                                                aaaaTAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTcc                                               >  2:1962686/1‑148 (MQ=255)
                                                                                                aaaaTAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTcc                                               <  1:1703997/148‑1 (MQ=255)
                                                                                                    taatTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGcc                                           <  2:2556861/148‑1 (MQ=255)
                                                                                                    taatTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGcc                                           >  1:2844399/1‑148 (MQ=255)
                                                                                                    taatTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGcc                                           <  2:3477200/148‑1 (MQ=255)
                                                                                                    taatTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCAAGcc                                           >  1:687665/1‑148 (MQ=255)
                                                                                                            aGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTa                                   >  2:1489519/1‑148 (MQ=255)
                                                                                                             gTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTaa                                  <  1:1561833/148‑1 (MQ=255)
                                                                                                               tCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATg                                >  2:3483787/1‑148 (MQ=255)
                                                                                                                cacaGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGc                               <  1:1466454/148‑1 (MQ=255)
                                                                                                                 acaGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCa                              <  2:401744/148‑1 (MQ=255)
                                                                                                                   aGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATg                            >  2:2708687/1‑148 (MQ=255)
                                                                                                                     ccTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTTACTTCATCCGGTACCTGCCAATGCGGACTAGTCCAGCCTGGCAGTAATGCATGAc                          >  1:1008304/1‑148 (MQ=255)
                                                                                                                     ccTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGAc                          >  1:508505/1‑148 (MQ=255)
                                                                                                                        tCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTgg                       >  2:125139/1‑148 (MQ=255)
                                                                                                                        tCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTgg                       >  2:3470458/1‑148 (MQ=255)
                                                                                                                            cTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGt                   >  2:1186257/1‑148 (MQ=255)
                                                                                                                             tGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTg                  <  2:3121244/148‑1 (MQ=255)
                                                                                                                              gCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGa                 <  2:2424647/148‑1 (MQ=255)
                                                                                                                              gCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGa                 >  1:1240078/1‑148 (MQ=255)
                                                                                                                                  gtgtCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAg             >  1:3182294/1‑148 (MQ=255)
                                                                                                                                   tgtCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAgg            >  1:1331563/1‑148 (MQ=255)
                                                                                                                                   tgtCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAgg            <  2:1868077/148‑1 (MQ=255)
                                                                                                                                    gtCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGt           <  1:1871896/148‑1 (MQ=255)
                                                                                                                                    gtCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGt           <  1:1866496/148‑1 (MQ=255)
                                                                                                                                         cTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTtct      <  2:2230505/148‑1 (MQ=255)
                                                                                                                                           gTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTcc    <  1:2881704/148‑1 (MQ=255)
                                                                                                                                             aGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGa  <  1:2008916/148‑1 (MQ=255)
                                                                                                                                             aGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGa  <  1:2355224/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGA  >  CP009273/461354‑461642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑