Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 480,820 C→T 100% D86N (GAC→AAC)  acrA ← multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273480,8200CT100.0% 741.9 / ‑6.7 223D86N (GAC→AAC) acrAmultidrug efflux system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (123/99);  minor base A (1/0);  total (124/99)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 223 total aligned reads displayed.

CCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGG  >  CP009273/480675‑480964
                                                                                                                                                 |                                                                                                                                                
ccGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGc                                                                                                                                                <  1:738188/148‑1 (MQ=255)
      aGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACctt                                                                                                                                          <  2:532857/148‑1 (MQ=255)
       gTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACcttc                                                                                                                                         >  1:2623027/1‑148 (MQ=255)
        tttCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACcttct                                                                                                                                        <  2:2453417/148‑1 (MQ=255)
         ttCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACcttctt                                                                                                                                       <  2:546855/148‑1 (MQ=255)
          tCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCttt                                                                                                                                      <  2:2727990/148‑1 (MQ=255)
           cTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTg                                                                                                                                     <  2:446058/148‑1 (MQ=255)
              aTAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGaaa                                                                                                                                  >  1:2764607/1‑148 (MQ=255)
               tAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAAt                                                                                                                                 <  2:2695382/148‑1 (MQ=255)
                aaCGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAAtt                                                                                                                                <  1:2993657/148‑1 (MQ=255)
                aaCGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAAtt                                                                                                                                <  1:1248235/148‑1 (MQ=255)
                   gATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACg                                                                                                                             <  1:987492/148‑1 (MQ=255)
                    aTTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGc                                                                                                                            <  1:3171733/148‑1 (MQ=255)
                     ttCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCt                                                                                                                           >  2:615514/1‑148 (MQ=255)
                      tCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCtt                                                                                                                          <  2:1738136/148‑1 (MQ=255)
                      tCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCtt                                                                                                                          >  1:1137573/1‑148 (MQ=255)
                       cACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTc                                                                                                                         >  1:928088/1‑148 (MQ=255)
                        aCCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCa                                                                                                                        <  2:1191118/148‑1 (MQ=255)
                        aCCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCa                                                                                                                        <  1:22971/148‑1 (MQ=255)
                        aCCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCa                                                                                                                        <  2:489260/148‑1 (MQ=255)
                          cGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAgg                                                                                                                      <  1:2835699/148‑1 (MQ=255)
                            tCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGAt                                                                                                                    >  1:3071056/1‑148 (MQ=255)
                                ttGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATc                                                                                                                <  1:1650113/148‑1 (MQ=255)
                                ttGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACAACTGCTTCGATGTAGCTACCTTATTTGAAATTACGCTTCAGGATAATc                                                                                                                >  2:281887/1‑148 (MQ=255)
                                     cgATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCt                                                                                                           >  2:2534213/1‑148 (MQ=255)
                                        tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAAc                                                                                                        >  1:2095392/1‑148 (MQ=255)
                                         atTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACt                                                                                                       >  1:2648229/1‑148 (MQ=255)
                                           tGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTg                                                                                                     >  1:1328533/1‑148 (MQ=255)
                                           tGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTg                                                                                                     <  2:3212540/148‑1 (MQ=255)
                                             gCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTCACTGGAg                                                                                                   >  1:2679345/1‑148 (MQ=255)
                                             gCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAg                                                                                                   >  1:3498544/1‑148 (MQ=255)
                                             gCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAg                                                                                                   >  1:2508337/1‑148 (MQ=255)
                                              cTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAgg                                                                                                  <  1:191361/148‑1 (MQ=255)
                                                 cAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACg                                                                                               >  2:1434662/1‑148 (MQ=255)
                                                  aGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGa                                                                                              <  2:3409567/148‑1 (MQ=255)
                                                   gCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGaa                                                                                             <  1:1790054/148‑1 (MQ=255)
                                                     cTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACt                                                                                           <  2:3021917/148‑1 (MQ=255)
                                                       gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTc                                                                                         >  1:25094/1‑148 (MQ=255)
                                                        ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCt                                                                                        >  2:241956/1‑148 (MQ=255)
                                                            ttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGa                                                                                    >  2:1374818/1‑148 (MQ=255)
                                                                ccAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                                                                >  2:878532/1‑148 (MQ=255)
                                                                  aGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                                                              <  2:1629623/148‑1 (MQ=255)
                                                                    aTCACCTTTCGCGCTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAg                                                                            >  2:1228095/1‑148 (MQ=255)
                                                                         cTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                                                                       <  1:602804/148‑1 (MQ=255)
                                                                                 cTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAc                                                               <  1:1002250/148‑1 (MQ=255)
                                                                                   gTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAccc                                                             >  2:356013/1‑148 (MQ=255)
                                                                                     cGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg                                                           >  2:2966569/1‑148 (MQ=255)
                                                                                       tATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGaa                                                         >  1:752089/1‑148 (MQ=255)
                                                                                       tATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGaa                                                         >  1:1244114/1‑148 (MQ=255)
                                                                                             gCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg                                                   <  2:84921/148‑1 (MQ=255)
                                                                                              ccTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGt                                                  <  2:3243767/148‑1 (MQ=255)
                                                                                               cTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt                                                 <  1:2461029/148‑1 (MQ=255)
                                                                                               cTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt                                                 >  2:1832499/1‑148 (MQ=255)
                                                                                                 gATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgt                                               <  1:2152064/148‑1 (MQ=255)
                                                                                                 gATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgt                                               >  2:220885/1‑148 (MQ=255)
                                                                                                 gATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgt                                               >  2:935961/1‑148 (MQ=255)
                                                                                                 gATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgt                                               <  2:1508737/148‑1 (MQ=255)
                                                                                                       tCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTg                                         <  2:1358323/148‑1 (MQ=255)
                                                                                                            ggATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCagag                                    >  2:1884933/1‑148 (MQ=255)
                                                                                                             gATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAgg                                   <  2:201400/148‑1 (MQ=255)
                                                                                                             gATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAgg                                   >  2:2087703/1‑148 (MQ=255)
                                                                                                               tCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGtt                                 >  1:3070296/1‑148 (MQ=255)
                                                                                                                cAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTc                                >  2:3144461/1‑148 (MQ=255)
                                                                                                                cAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTc                                >  2:3281624/1‑148 (MQ=255)
                                                                                                                cAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTc                                >  1:1023747/1‑148 (MQ=255)
                                                                                                                  aTCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAg                              <  1:3493489/148‑1 (MQ=255)
                                                                                                                   tCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGt                             >  2:3305930/1‑148 (MQ=255)
                                                                                                                    cTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGtt                            >  1:979588/1‑148 (MQ=255)
                                                                                                                     tGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGttt                           <  2:1475927/148‑1 (MQ=255)
                                                                                                                     tGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGttt                           <  1:2674306/148‑1 (MQ=255)
                                                                                                                      gATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGtttt                          >  1:3352630/1‑148 (MQ=255)
                                                                                                                       aTAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTg                         <  1:1018813/148‑1 (MQ=255)
                                                                                                                        tAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGa                        >  2:1313002/1‑148 (MQ=255)
                                                                                                                         agagagagACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGAc                       >  2:671198/1‑148 (MQ=255)
                                                                                                                         agagagagACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGAc                       >  2:2385665/1‑148 (MQ=255)
                                                                                                                           agagagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTg                     <  1:1441585/148‑1 (MQ=255)
                                                                                                                            gagagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGt                    >  2:2330634/1‑148 (MQ=255)
                                                                                                                            gagagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGt                    >  1:3206195/1‑148 (MQ=255)
                                                                                                                            gagagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGt                    <  1:330506/148‑1 (MQ=255)
                                                                                                                              gagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTta                  <  2:2643482/148‑1 (MQ=255)
                                                                                                                              gagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTta                  >  1:1087973/1‑148 (MQ=255)
                                                                                                                               agaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTtac                 >  1:2540312/1‑148 (MQ=255)
                                                                                                                                gaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTtact                <  1:2470905/148‑1 (MQ=255)
                                                                                                                                gaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTtact                <  1:176830/148‑1 (MQ=255)
                                                                                                                                  aacCTGCTTCGATGTTGCTACATTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGTACGAACTTCTGCGATCCGGTAGGCACAGGTGCGACCAGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTCTtactac              >  2:732773/2‑148 (MQ=255)
                                                                                                                                    ccTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGc            >  2:1342334/1‑148 (MQ=255)
                                                                                                                                    ccTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGc            >  2:1962557/1‑148 (MQ=255)
                                                                                                                                    ccTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGc            >  2:2947301/1‑148 (MQ=255)
                                                                                                                                      tGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCa          >  1:883223/1‑148 (MQ=255)
                                                                                                                                      tGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCa          <  2:2578337/148‑1 (MQ=255)
                                                                                                                                       gCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCaa         <  2:1531515/148‑1 (MQ=255)
                                                                                                                                         ttCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcg       >  2:1980050/1‑148 (MQ=255)
                                                                                                                                          tCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcgg      <  1:402841/148‑1 (MQ=255)
                                                                                                                                          tCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcgg      <  1:1709048/148‑1 (MQ=255)
                                                                                                                                           cGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcggc     >  2:2727271/1‑148 (MQ=255)
                                                                                                                                              tGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCggg  >  2:2629263/1‑148 (MQ=255)
                                                                                                                                              tGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCggg  >  2:824149/1‑148 (MQ=255)
                                                                                                                                              tGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCggg  >  2:2870240/1‑148 (MQ=255)
                                                                                                                                              tGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCggg  >  1:552519/1‑148 (MQ=255)
                                                                                                                                              tGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCggg  >  2:1089316/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGG  >  CP009273/480675‑480964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑