| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 482,757 | G→T | 100% | A256S (GCG→TCG) | mscK → | mechanosensitive channel protein, intermediate conductance, K+ regulated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 482,757 | 0 | G | T | 98.9% | 552.0 / ‑1.7 | 176 | A256S (GCG→TCG) | mscK | mechanosensitive channel protein, intermediate conductance, K+ regulated |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (89/85); total (90/86) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 180 total aligned reads displayed.
GCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCG > CP009273/482612‑482901 | gcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCg < 1:636595/148‑1 (MQ=255)gcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCg < 1:2392816/148‑1 (MQ=255)gcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCg < 1:2392815/148‑1 (MQ=255) agGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGt < 2:2026966/148‑1 (MQ=255) ggCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTa > 2:1624442/1‑148 (MQ=255) gCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTaa > 1:2661976/1‑148 (MQ=255) ttGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACa < 2:2339158/148‑1 (MQ=255) tGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAg > 2:231529/1‑148 (MQ=255) gCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGc < 2:3208119/148‑1 (MQ=255) gCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGc < 2:587330/148‑1 (MQ=255) tGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCaa < 2:1575450/148‑1 (MQ=255) gCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGcc < 2:2067057/148‑1 (MQ=255) ggAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTg < 2:3037257/148‑1 (MQ=255) ggAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTg < 2:2967241/148‑1 (MQ=255) agaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGAc > 1:1754559/1‑148 (MQ=255) agaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGAc > 2:1817168/1‑148 (MQ=255) agaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCGTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGAc < 2:2584179/148‑1 (MQ=255) aTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACCGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACtt > 2:3357151/1‑148 (MQ=255) ttGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACttt > 2:354581/1‑148 (MQ=255) tGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTa > 2:763263/1‑148 (MQ=255) gACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTaa < 2:2391311/148‑1 (MQ=255) aCCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAAc < 2:1395897/148‑1 (MQ=255) cGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑aaaaaa > 2:1739048/1‑148 (MQ=255) tAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACg > 2:2223507/1‑148 (MQ=255) aaaaGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACgg > 2:116594/1‑148 (MQ=255) aaaaGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACgg > 1:1156883/1‑148 (MQ=255) aaGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGgcg > 1:3067567/1‑148 (MQ=255) aaGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGgcg > 1:1433837/1‑148 (MQ=255) aaGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGgcg > 1:1561241/1‑148 (MQ=255) aaGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGgcg > 1:2909380/1‑148 (MQ=255) aGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGgcgc > 2:1082635/1‑148 (MQ=255) cTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAgg < 2:2605626/148‑1 (MQ=255) tGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGa < 1:210559/148‑1 (MQ=255) gAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAg > 1:2938673/1‑148 (MQ=255) aaGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGc < 2:3336216/148‑1 (MQ=255) aaGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGc < 2:3336030/148‑1 (MQ=255) gggAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCg > 1:95791/1‑148 (MQ=255) ggATCACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGt < 2:751333/148‑1 (MQ=255) aaCACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAAACGGCGCAGGAAGCCGtc > 2:2850530/1‑148 (MQ=255) acCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTccc > 1:3030876/1‑148 (MQ=255) ccGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTcccc > 1:151334/1‑148 (MQ=255) cTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGAt > 2:226160/1‑148 (MQ=255) cAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAg > 2:1277644/1‑148 (MQ=255) cAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAg < 1:1474789/148‑1 (MQ=255) cAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAg > 2:3041929/1‑148 (MQ=255) gATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcg > 1:2646242/1‑148 (MQ=255) ccTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcgcgcg > 1:2747784/1‑148 (MQ=255) ttGCAAAAGCAACGTGATTACGTGACGGCGAACCGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTa < 2:954857/148‑1 (MQ=255) tGCAAAAGCAAGGTGTTTCCGTGACGGCGAACCGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTAt < 1:3328477/148‑1 (MQ=255) gCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGTAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTAtt < 1:3126733/148‑1 (MQ=255) aaaGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAg > 2:1128362/1‑148 (MQ=255) aaaGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAg < 1:1327494/148‑1 (MQ=255) aaaGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAg > 2:1496350/1‑148 (MQ=255) aaGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAgg < 2:1253720/148‑1 (MQ=255) aGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGc > 2:315775/1‑148 (MQ=255) gCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCt > 2:3081947/1‑148 (MQ=255) gCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCt > 2:3254461/1‑148 (MQ=255) catcGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTTCACCTGTTTCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTa < 2:3319108/145‑1 (MQ=255) cAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTa < 2:3319133/148‑1 (MQ=255) aaCGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTaa < 1:1936834/148‑1 (MQ=255) aCGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAAt > 1:2487956/1‑148 (MQ=255) aCGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAAt > 1:2488129/1‑148 (MQ=255) gATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGc < 2:1779016/148‑1 (MQ=255) tACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTgg > 1:1891817/1‑148 (MQ=255) tGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGaa < 2:881912/148‑1 (MQ=255) gCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAgg < 2:50070/148‑1 (MQ=255) gCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAgg > 2:881831/1‑148 (MQ=255) gCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAgg < 2:1041084/148‑1 (MQ=255) gCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAgg < 2:2796043/148‑1 (MQ=255) aaCAGCGCGCGTCTGGCGCACCAGTTACAACTGTTGAAAGAATCGGTAAACAGCACGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAAc < 1:2455451/148‑1 (MQ=255) cAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACCGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTg < 2:1608986/148‑1 (MQ=255) cAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTg > 1:437769/1‑148 (MQ=255) cAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTg > 2:2354077/1‑148 (MQ=255) aGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTgg < 1:3477110/148‑1 (MQ=255) aGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTgg < 2:2524680/148‑1 (MQ=255) cgcTCGTCTGTAGCACCATTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGaa < 2:1577414/148‑1 (MQ=255) cTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAAt < 1:788306/148‑1 (MQ=255) cTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAcc > 1:2328401/1‑148 (MQ=255) ggAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACcag < 2:63491/148‑1 (MQ=255) aGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACcagca > 2:2118495/1‑148 (MQ=255) gccccAGTTACAACTGGTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACcagcag < 2:2316927/145‑1 (MQ=255) aCCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGtt < 2:3193381/148‑1 (MQ=255) aCCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGtt < 1:1832603/148‑1 (MQ=255) ccAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTa < 1:1082635/148‑1 (MQ=255) ccAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTa > 2:717063/1‑148 (MQ=255) cAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTaa > 2:3472771/1‑148 (MQ=255) aGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAg < 2:1416780/148‑1 (MQ=255) aGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAg < 1:1499309/148‑1 (MQ=255) gTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGt > 1:2396247/1‑148 (MQ=255) gTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGt > 1:1236499/1‑148 (MQ=255) gTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGt > 1:633672/1‑148 (MQ=255) ttACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTc < 2:3132008/148‑1 (MQ=255) ttACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTc > 1:523520/1‑148 (MQ=255) aCAAGTGTTGCAATAATCGGTAACCCGCAAGCGCGTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAg < 1:38014/148‑1 (MQ=255) aaCTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCg > 1:344509/1‑148 (MQ=255) cTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTc > 2:691535/1‑148 (MQ=255) cTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTc > 1:2426207/1‑148 (MQ=255) aagaatCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTAc > 1:448970/1‑148 (MQ=255) agaatCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTAcc > 2:1843472/1‑148 (MQ=255) gaatCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACcg > 2:1969616/1‑148 (MQ=255) | GCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCG‑AAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCG > CP009273/482612‑482901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |