Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 518,820 C→A 100% A35E (GCG→GAG)  rhsD → Rhs family putative polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273518,8200CA98.9% 641.3 / ‑1.8 199A35E (GCG→GAG) rhsDRhs family putative polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (103/93);  total (104/95)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 207 total aligned reads displayed.

CTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGAC  >  CP009273/518676‑518964
                                                                                                                                                |                                                                                                                                                
cTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGgcgtggagtg                                                                                                                                               >  1:192610/1‑148 (MQ=255)
 tAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCTCGCCCACCGGCGTGGAGTGc                                                                                                                                              >  1:1583307/1‑148 (MQ=255)
 tAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGc                                                                                                                                              <  1:897528/148‑1 (MQ=255)
 tAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGc                                                                                                                                              >  1:1807578/1‑148 (MQ=255)
   acacAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTc                                                                                                                                            >  1:233194/1‑148 (MQ=255)
     acaGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCgg                                                                                                                                          <  1:2974672/148‑1 (MQ=255)
      caGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgt                                                                                                                                         <  1:3246732/148‑1 (MQ=255)
        gggATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtgt                                                                                                                                       >  1:2569470/1‑148 (MQ=255)
        gggATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtgt                                                                                                                                       <  1:2654576/148‑1 (MQ=255)
        gggATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtgt                                                                                                                                       <  1:1408029/148‑1 (MQ=255)
          gATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGgtgtgt                                                                                                                                     <  2:3026426/148‑1 (MQ=255)
           atatGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTc                                                                                                                                    <  1:93757/148‑1 (MQ=255)
            tatGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTcc                                                                                                                                   <  2:1790491/148‑1 (MQ=255)
             atGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcg                                                                                                                                  >  1:184847/1‑148 (MQ=255)
             atGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcg                                                                                                                                  >  2:2623001/1‑148 (MQ=255)
             atGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcg                                                                                                                                  >  1:184777/1‑148 (MQ=255)
               gAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcggg                                                                                                                                >  1:1318356/1‑148 (MQ=255)
                   aaTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcgggcgtg                                                                                                                            <  1:1532653/148‑3 (MQ=255)
                   aaTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcgggcggg                                                                                                                            <  2:2814453/148‑1 (MQ=255)
                   aaTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCcgggcggg                                                                                                                            <  2:2479677/148‑1 (MQ=255)
                    aTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGa                                                                                                                           >  2:3100988/1‑148 (MQ=255)
                    aTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGa                                                                                                                           >  1:1268876/1‑148 (MQ=255)
                           aaaGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTc                                                                                                                    >  1:3482107/1‑148 (MQ=255)
                            aaGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCg                                                                                                                   <  2:2865219/148‑1 (MQ=255)
                             aGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCgg                                                                                                                  >  2:1172812/1‑148 (MQ=255)
                             aGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCgg                                                                                                                  >  1:2880739/1‑148 (MQ=255)
                              gggCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCggg                                                                                                                 <  1:901016/148‑1 (MQ=255)
                                 cACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCaa                                                                                                              <  2:1409788/148‑1 (MQ=255)
                                 cACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCaa                                                                                                              >  2:3439/1‑148 (MQ=255)
                                 cACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCaa                                                                                                              >  2:1574639/1‑148 (MQ=255)
                                  aCTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAAc                                                                                                             >  1:1885530/1‑148 (MQ=255)
                                  aCTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAAc                                                                                                             >  1:832842/1‑148 (MQ=255)
                                   cTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAAcc                                                                                                            <  1:3126452/148‑1 (MQ=255)
                                   cTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAAcc                                                                                                            >  1:1106326/1‑148 (MQ=255)
                                         atGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaa                                                                                                      >  1:3297702/1‑148 (MQ=255)
                                         atGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaa                                                                                                      <  1:2689010/148‑1 (MQ=255)
                                         atGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaa                                                                                                      <  2:763828/148‑1 (MQ=255)
                                         atGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaa                                                                                                      >  1:144785/1‑148 (MQ=255)
                                          tGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTaaa                                                                                                     <  2:1248325/148‑1 (MQ=255)
                                          tGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCAGGTaaa                                                                                                     <  2:1971433/148‑1 (MQ=255)
                                            aGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATc                                                                                                   <  2:1672178/148‑1 (MQ=255)
                                              cGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCg                                                                                                 >  1:2420684/1‑148 (MQ=255)
                                              cGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCg                                                                                                 <  1:3469032/148‑1 (MQ=255)
                                                gAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgct                                                                                               >  1:2539953/1‑148 (MQ=255)
                                                 aaaaCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgctg                                                                                              >  1:1254596/1‑148 (MQ=255)
                                                 aaaaCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgctg                                                                                              <  1:1368147/148‑1 (MQ=255)
                                                  cacccAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCgctgc                                                                                             <  2:2000589/145‑1 (MQ=255)
                                                    aCCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGctgctg                                                                                           <  1:666368/148‑1 (MQ=255)
                                                     ccAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTgg                                                                                          >  1:912637/1‑148 (MQ=255)
                                                      cAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTggg                                                                                         <  1:3336654/148‑1 (MQ=255)
                                                       aGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTgggg                                                                                        >  1:2170985/1‑148 (MQ=255)
                                                        gcggcgCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTggggg                                                                                       >  1:1158068/1‑148 (MQ=255)
                                                        gcggcgCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTggggg                                                                                       >  1:1156658/1‑148 (MQ=255)
                                                         cggcgCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGc                                                                                      <  1:374274/148‑1 (MQ=255)
                                                          ggcgCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCg                                                                                     <  1:2058488/148‑1 (MQ=255)
                                                             gcgTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAg                                                                                  <  1:1914029/148‑1 (MQ=255)
                                                                tCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtg                                                                               >  2:2171283/1‑148 (MQ=255)
                                                                tCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtg                                                                               >  2:1334824/1‑148 (MQ=255)
                                                                 cAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgc                                                                              >  2:1213603/1‑148 (MQ=255)
                                                                 cAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgc                                                                              >  2:1320924/1‑148 (MQ=255)
                                                                   gggAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgctg                                                                            >  1:862931/1‑148 (MQ=255)
                                                                    ggAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgctgc                                                                           >  1:100077/1‑148 (MQ=255)
                                                                    ggAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgctgc                                                                           >  1:100041/1‑148 (MQ=255)
                                                                    ggAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGtgctgc                                                                           <  1:3054803/148‑1 (MQ=255)
                                                                     gagaTATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGcc                                                                          >  2:409436/1‑148 (MQ=255)
                                                                        atatGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTACGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCgg                                                                       >  1:255044/1‑148 (MQ=255)
                                                                        atatGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCgg                                                                       >  1:255124/1‑148 (MQ=255)
                                                                        atatGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCgg                                                                       <  1:582646/148‑1 (MQ=255)
                                                                         tatGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGAGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGc                                                                      >  1:2358699/1‑148 (MQ=255)
                                                                          atGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCg                                                                     <  2:503565/148‑1 (MQ=255)
                                                                          atGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCg                                                                     >  2:1286875/1‑148 (MQ=255)
                                                                              cTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGAc                                                                 >  2:2068657/1‑148 (MQ=255)
                                                                                cAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACgg                                                               <  1:1037766/148‑1 (MQ=255)
                                                                                  gTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGAc                                                             >  2:1770393/1‑148 (MQ=255)
                                                                                   tATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGAcc                                                            <  2:192610/148‑1 (MQ=255)
                                                                                    aTGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCt                                                           <  2:533712/148‑1 (MQ=255)
                                                                                              cATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgccc                                                 >  1:436726/1‑148 (MQ=255)
                                                                                                 tGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccggc                                              >  1:1772906/1‑148 (MQ=255)
                                                                                                 tGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccggc                                              >  2:3410976/1‑148 (MQ=255)
                                                                                                    ccAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccggcccg                                           <  2:2841537/148‑1 (MQ=255)
                                                                                                     cAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGc                                          >  2:682323/1‑148 (MQ=255)
                                                                                                     cAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGc                                          >  2:231740/1‑148 (MQ=255)
                                                                                                       gggTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTg                                        <  1:280644/148‑1 (MQ=255)
                                                                                                         gTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGcc                                      >  1:1205710/1‑148 (MQ=255)
                                                                                                                aGGTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCAtt                               <  2:2754004/148‑1 (MQ=255)
                                                                                                                 ggTGTAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtc                              <  1:1827819/148‑1 (MQ=255)
                                                                                                                     tAAGAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctctc                          <  1:1412834/148‑1 (MQ=255)
                                                                                                                        gAATTGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCg                       >  1:2044435/1‑148 (MQ=255)
                                                                                                                            tGGCGCGCCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCAcc                   <  1:600311/148‑1 (MQ=255)
                                                                                                                              gcgcgcCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGCCGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTa                 <  1:169395/148‑1 (MQ=255)
                                                                                                                               cgcgcCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAc                <  2:1081337/148‑1 (MQ=255)
                                                                                                                                 cgcCCACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTAcag              <  1:2276045/148‑1 (MQ=255)
                                                                                                                                     cACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACagcagc          <  2:297073/148‑1 (MQ=255)
                                                                                                                                     cACCGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACagcagc          >  1:3415879/1‑148 (MQ=255)
                                                                                                                                      aCCGGCGTGGACTGCTCGGTGTGTCCGGGCGGGATGAATTCGGGCAACCCGGTAAAACCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTATCCCCCACCTACAACATCt         >  1:1832567/1‑148 (MQ=25)
                                                                                                                                       ccGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTa        <  2:188014/148‑1 (MQ=255)
                                                                                                                                       ccGGCGTGGAGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTa        <  1:3130370/148‑1 (MQ=255)
                                                                                                                                          gcgtggagtgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCg     <  2:255124/148‑1 (MQ=255)
                                                                                                                                          gcgtggagtgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCg     <  2:255044/148‑1 (MQ=255)
                                                                                                                                             tggagtgCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGAc  >  2:1147993/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAGAATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGTCCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGCACCTACAGCAGCTACCGGAC  >  CP009273/518676‑518964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑