Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 581,633 G→T 90.0% L244L (CTC→CTA)  envY ← porin thermoregulatory transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273581,6330GT90.0% 499.6 / 50.6 197L244L (CTC→CTA) envYporin thermoregulatory transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (7/13);  new base T (86/91);  total (93/104)
Fisher's exact test for biased strand distribution p-value = 3.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 200 total aligned reads displayed.

TTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACT  >  CP009273/581488‑581778
                                                                                                                                                 |                                                                                                                                                 
ttgACTTAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCtag                                                                                                                                                 >  2:1260882/1‑148 (MQ=255)
ttgACTTAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCtag                                                                                                                                                 <  2:497940/148‑1 (MQ=255)
  gACTTAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGAt                                                                                                                                               <  2:1144572/148‑1 (MQ=255)
   aCTTAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATa                                                                                                                                              <  1:2550805/148‑1 (MQ=255)
   aCTTAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATa                                                                                                                                              >  1:1929913/1‑148 (MQ=255)
     ttAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGTAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAAt                                                                                                                                            <  2:811419/148‑1 (MQ=255)
     ttAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAAt                                                                                                                                            >  2:1094882/1‑148 (MQ=255)
      tAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAAtt                                                                                                                                           <  1:1395284/148‑1 (MQ=255)
      tAAAATGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTAATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAAtt                                                                                                                                           >  1:1475543/1‑148 (MQ=255)
        aaaTGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCa                                                                                                                                         <  1:2619115/148‑1 (MQ=255)
        aaaTGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCa                                                                                                                                         <  1:1660798/148‑1 (MQ=255)
         aaTGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCaa                                                                                                                                        >  2:391130/1‑148 (MQ=255)
         aaTGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCaa                                                                                                                                        >  1:3009982/1‑148 (MQ=255)
         aaTGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCaa                                                                                                                                        >  1:1261199/1‑148 (MQ=255)
           tGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACg                                                                                                                                      >  1:1813180/1‑148 (MQ=255)
           tGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACg                                                                                                                                      <  1:123602/148‑1 (MQ=255)
           tGTCTTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACg                                                                                                                                      <  2:309184/148‑1 (MQ=255)
                 tCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCa                                                                                                                                <  1:2536908/148‑1 (MQ=255)
                   tAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAgg                                                                                                                              <  2:955133/148‑1 (MQ=255)
                    aGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATACGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGc                                                                                                                             >  1:1752014/1‑148 (MQ=255)
                     ggAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGcc                                                                                                                            >  2:1192794/1‑148 (MQ=255)
                     ggAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGcc                                                                                                                            >  2:291903/1‑148 (MQ=255)
                           tCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTaaaa                                                                                                                      >  2:730386/1‑148 (MQ=255)
                             tAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACg                                                                                                                    >  1:724361/1‑148 (MQ=255)
                              aaGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGc                                                                                                                   <  1:1569969/148‑1 (MQ=255)
                              aaGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGc                                                                                                                   >  1:962156/1‑148 (MQ=255)
                               aGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGcc                                                                                                                  >  1:3088465/1‑148 (MQ=255)
                                 tCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCtt                                                                                                                >  1:2134354/1‑148 (MQ=255)
                                     gggCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTaaaa                                                                                                            <  2:775557/148‑1 (MQ=255)
                                       gCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCAAATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAAc                                                                                                          <  2:3356670/148‑1 (MQ=255)
                                         cGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACag                                                                                                        <  1:2384219/148‑1 (MQ=255)
                                         cGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACag                                                                                                        <  1:2247534/148‑1 (MQ=255)
                                              gTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATa                                                                                                   <  1:530798/148‑1 (MQ=255)
                                              gTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATa                                                                                                   >  2:1219060/1‑148 (MQ=255)
                                              gTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTATTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATa                                                                                                   >  2:2144115/1‑148 (MQ=255)
                                               ttCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCTCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATaa                                                                                                  <  1:1154332/148‑1 (MQ=255)
                                               ttCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATaa                                                                                                  <  1:59925/148‑1 (MQ=255)
                                                 cGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAg                                                                                                <  2:962156/148‑1 (MQ=255)
                                                 cGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAg                                                                                                <  1:2670918/148‑1 (MQ=255)
                                                  gAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGTCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGt                                                                                               <  2:2790984/148‑1 (MQ=255)
                                                        tGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTacgacg                                                                                         <  1:503985/148‑1 (MQ=255)
                                                         gCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGt                                                                                        >  1:3371927/1‑148 (MQ=255)
                                                           aGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGtgc                                                                                      <  1:673655/148‑1 (MQ=255)
                                                             ggCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGtgctg                                                                                    >  2:404608/1‑148 (MQ=255)
                                                             ggCGCGCCATTTCTGCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGtgctg                                                                                    <  2:2537307/148‑1 (MQ=255)
                                                                  gcCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCtata                                                                               <  2:2605449/148‑1 (MQ=255)
                                                                     aTTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGcc                                                                            <  1:828005/148‑1 (MQ=255)
                                                                     aTTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGcc                                                                            >  2:356392/1‑148 (MQ=255)
                                                                     aTTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGcc                                                                            <  1:2097465/148‑1 (MQ=255)
                                                                     aTTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGACATTCACCACATCACAATAACACGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGcc                                                                            >  1:3448069/1‑148 (MQ=255)
                                                                         cttcCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACAt                                                                        >  2:2008912/1‑148 (MQ=255)
                                                                         cttcCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACAt                                                                        >  2:1044010/1‑148 (MQ=255)
                                                                           tcCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTAGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATaa                                                                      >  2:2364044/1‑148 (MQ=255)
                                                                            ccTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGTTAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAAt                                                                     <  1:910459/148‑1 (MQ=255)
                                                                            ccTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAAt                                                                     <  2:2998960/148‑1 (MQ=255)
                                                                              tCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTg                                                                   <  2:777493/148‑1 (MQ=255)
                                                                              tCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTg                                                                   <  2:1339567/148‑1 (MQ=255)
                                                                              tCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTg                                                                   <  2:2377048/148‑1 (MQ=255)
                                                                              tCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTg                                                                   >  2:690228/1‑148 (MQ=255)
                                                                               cATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAAc                                                                                                          >  2:118046/1‑108 (MQ=255)
                                                                               cATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAAc                                                                                                          <  1:118046/108‑1 (MQ=255)
                                                                               cATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTgc                                                                  >  2:4474/1‑148 (MQ=255)
                                                                                 tttATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTgcgc                                                                <  1:36621/148‑1 (MQ=255)
                                                                                   tATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCa                                                              >  2:1717651/1‑148 (MQ=255)
                                                                                       cccGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTg                                                          <  2:675618/148‑1 (MQ=255)
                                                                                              tATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGata                                                   <  2:3083378/148‑1 (MQ=255)
                                                                                               aTCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGatat                                                  >  1:2924227/1‑148 (MQ=255)
                                                                                               aTCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGatat                                                  <  2:263983/148‑1 (MQ=255)
                                                                                               aTCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGatat                                                  <  2:1929913/148‑1 (MQ=255)
                                                                                               aTCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGatat                                                  <  2:2586128/148‑1 (MQ=255)
                                                                                               aTCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGatat                                                  <  1:729367/148‑1 (MQ=255)
                                                                                                  cGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATAtttt                                               <  2:2066442/148‑1 (MQ=255)
                                                                                                  cGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATAtttt                                               <  2:1679946/148‑1 (MQ=255)
                                                                                                    tttCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTg                                             <  1:764229/148‑1 (MQ=255)
                                                                                                     ttCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGt                                            >  2:3161886/1‑148 (MQ=255)
                                                                                                         gCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATc                                        >  2:2201734/1‑148 (MQ=255)
                                                                                                           tttGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCa                                      >  1:1601092/1‑148 (MQ=255)
                                                                                                            ttGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCAt                                     <  2:2505185/148‑1 (MQ=255)
                                                                                                            ttGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCAt                                     <  2:1261199/148‑1 (MQ=255)
                                                                                                            ttGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCAt                                     <  2:3009982/148‑1 (MQ=255)
                                                                                                                 cTTCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATa                                >  1:1001267/1‑148 (MQ=255)
                                                                                                                  ttCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATaa                               <  2:740590/148‑1 (MQ=255)
                                                                                                                   tCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTTCGCCACCTGAGTGATATTTTTGTTATCCATCAATaaa                              >  1:93722/1‑147 (MQ=255)
                                                                                                                   tCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAAc                              >  1:695851/1‑148 (MQ=255)
                                                                                                                   tCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAAc                              <  1:2145728/148‑1 (MQ=255)
                                                                                                                   tCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAAc                              <  1:377651/148‑1 (MQ=255)
                                                                                                                   tCACCACATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAAc                              >  1:93751/1‑148 (MQ=255)
                                                                                                                    caccacATCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACa                             >  1:126494/1‑148 (MQ=255)
                                                                                                                       cacaTCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCt                          <  2:1752014/148‑1 (MQ=255)
                                                                                                                        acaTCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTg                         >  2:3251927/1‑148 (MQ=255)
                                                                                                                         caTCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGt                        <  1:2899186/148‑1 (MQ=255)
                                                                                                                         caTCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGt                        >  2:3402050/1‑148 (MQ=255)
                                                                                                                           tCACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTAc                      >  1:1998226/1‑148 (MQ=255)
                                                                                                                            cACTTTTTGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACg                     >  1:2309875/1‑148 (MQ=255)
                                                                                                                                 tttGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTa                <  1:1555678/148‑1 (MQ=255)
                                                                                                                                 tttGTCGCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTa                <  1:3296529/148‑1 (MQ=255)
                                                                                                                                       gCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCAt          <  2:1011075/148‑1 (MQ=255)
                                                                                                                                       gCTGTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCAt          <  1:3161886/148‑1 (MQ=255)
                                                                                                                                          gTTTGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACg       <  2:769292/148‑1 (MQ=255)
                                                                                                                                            ttGGCTAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGac     >  2:2583612/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCTTAAAAACAGAGATAAAGTACGACGTGCTGCTATAGCCACATAATTGCGCCACCTGAGTGATATTTTTGTTATCCATCAATAACATCTGTACGGCGTAACGCATACGACACT  >  CP009273/581488‑581778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑