Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 80,123 C→T 100% G25S (GGC→AGC)  leuL ← leu operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927380,1230CT99.6% 705.3 / ‑5.9 214G25S (GGC→AGC) leuLleu operon leader peptide
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (104/108);  minor base G (1/0);  total (106/108)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 214 total aligned reads displayed.

CAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGA  >  CP009273/79981‑80264
                                                                                                                                              |                                                                                                                                             
cAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCa                                                                                                                                          >  1:3382250/1‑148 (MQ=255)
cAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCa                                                                                                                                          >  1:1815466/1‑148 (MQ=255)
      ggTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTc                                                                                                                                    >  1:451193/1‑148 (MQ=255)
        tATCGAAAATAATGACTTGCTTGCTCATGGTTTTGGTCATTGTCTCTTTTAGAGCGCCTAGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGATTAATGCTGGATGCTGCTCACTCGTCTa                                                                                                                                  <  2:1807444/148‑1 (MQ=255)
        tATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTa                                                                                                                                  >  1:2442696/1‑148 (MQ=255)
        tATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGATTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTa                                                                                                                                  >  1:2626245/1‑148 (MQ=255)
         aTCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTATTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTAc                                                                                                                                 <  2:3495120/148‑1 (MQ=255)
              aaaTAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCgcgc                                                                                                                            >  2:217621/1‑148 (MQ=255)
               aataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCa                                                                                                                           >  1:2197877/1‑148 (MQ=255)
                ataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCaa                                                                                                                          >  1:3234926/1‑148 (MQ=255)
                  aatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAAAGGCGCGGGTTTTTTTTTTGACTGAGTGCTGGCTTAATGCTGGATGCTGCTAACTCGTCTACCGCGCAaag                                                                                                                        >  1:903942/1‑148 (MQ=255)
                   atGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCATTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAaaga                                                                                                                       <  2:263931/148‑1 (MQ=255)
                     gACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAaagaag                                                                                                                     >  2:1739527/1‑148 (MQ=255)
                     gACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAaagaag                                                                                                                     >  2:1871638/1‑148 (MQ=255)
                     gACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAaagaag                                                                                                                     <  2:129645/148‑1 (MQ=255)
                        ttGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATg                                                                                                                  >  2:2821191/1‑148 (MQ=255)
                        ttGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGGTGGACGGCGTGGGGGGTTAGTGCTGGATGGGGGTCCCTCGGCTCCCGCGCCCAGACGATg                                                                                                                  >  2:958100/1‑148 (MQ=255)
                         tGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGc                                                                                                                 >  1:1585896/1‑148 (MQ=255)
                           cTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGt                                                                                                               <  1:3047370/148‑1 (MQ=255)
                              gCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTta                                                                                                            >  2:1320753/1‑148 (MQ=255)
                              gCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTta                                                                                                            >  2:1780315/1‑148 (MQ=255)
                                tCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt                                                                                                          <  1:735825/148‑1 (MQ=255)
                                 cATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagta                                                                                                         >  1:3252224/1‑148 (MQ=255)
                                 cATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagta                                                                                                         <  2:984918/148‑1 (MQ=255)
                                   tGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagt                                                                                                       >  1:1772820/1‑148 (MQ=255)
                                    ggTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagta                                                                                                      >  2:2010271/1‑148 (MQ=255)
                                        tGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagtag                                                                                                  >  2:3017562/1‑148 (MQ=255)
                                        tGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagtag                                                                                                  <  1:143610/148‑1 (MQ=255)
                                          ggTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGAc                                                                                                <  2:144272/148‑1 (MQ=255)
                                          ggTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGAc                                                                                                <  1:2891183/148‑1 (MQ=255)
                                              cTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGAt                                                                                            <  1:663572/148‑1 (MQ=255)
                                               ttGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATa                                                                                           >  1:260970/1‑148 (MQ=255)
                                                tGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaa                                                                                          >  1:1964652/1‑148 (MQ=255)
                                                tGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaa                                                                                          <  1:2821191/148‑1 (MQ=255)
                                                tGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaa                                                                                          <  2:2565802/148‑1 (MQ=255)
                                                tGTCTCTTTTAGAGCGCCTCGATTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaa                                                                                          <  1:988079/148‑1 (MQ=255)
                                                 gTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaaa                                                                                         >  1:1259660/1‑148 (MQ=255)
                                                       tttAGAGCGCCTCGCTTCGGGCATAAAAATACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAAc                                                                                   >  2:769764/1‑148 (MQ=255)
                                                       tttAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAAc                                                                                   >  2:732387/1‑148 (MQ=255)
                                                        ttAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACg                                                                                  >  1:392946/1‑148 (MQ=255)
                                                        ttAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACg                                                                                  <  2:1536252/148‑1 (MQ=255)
                                                          agagCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGAt                                                                                <  1:1777728/148‑1 (MQ=255)
                                                          agagCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGAt                                                                                <  1:1409115/148‑1 (MQ=255)
                                                          agagCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGAt                                                                                <  1:2604070/148‑1 (MQ=255)
                                                            agCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGAtgt                                                                              >  2:805353/1‑148 (MQ=255)
                                                            agCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTGTAGTAGTAGTAGACCGATAAAGCGAACGAtgt                                                                              >  2:618077/1‑148 (MQ=255)
                                                             gcgcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGAtgtg                                                                             >  2:1786535/1‑148 (MQ=255)
                                                               gcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAg                                                                           <  2:3048806/148‑1 (MQ=255)
                                                                ccTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGt                                                                          <  1:837775/148‑1 (MQ=255)
                                                                ccTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGt                                                                          <  2:3019692/148‑1 (MQ=255)
                                                                 cTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTc                                                                         >  2:2118238/1‑148 (MQ=255)
                                                                 cTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTc                                                                         <  2:1756123/148‑1 (MQ=255)
                                                                  tCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCa                                                                        <  1:1884310/148‑1 (MQ=255)
                                                                     cTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCTTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTa                                                                     >  1:2846354/1‑148 (MQ=255)
                                                                      ttCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTaa                                                                    >  2:3156899/1‑148 (MQ=255)
                                                                       tCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTaaa                                                                   >  2:2662908/1‑148 (MQ=255)
                                                                       tCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTaaa                                                                   <  1:2159455/148‑1 (MQ=255)
                                                                        cGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAAt                                                                  <  2:2863650/148‑1 (MQ=255)
                                                                        cGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAAt                                                                  <  2:2362976/148‑1 (MQ=255)
                                                                         gggCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATc                                                                 <  2:2626300/148‑1 (MQ=255)
                                                                            cATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGc                                                              >  1:3075764/1‑148 (MQ=255)
                                                                              tAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTATTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTc                                                            <  1:1644352/148‑1 (MQ=255)
                                                                                  aaaaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGa                                                        <  2:572995/148‑1 (MQ=255)
                                                                                    aaCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATg                                                      <  2:2749626/148‑1 (MQ=255)
                                                                                     aCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGa                                                     <  2:3076463/148‑1 (MQ=255)
                                                                                      cccGCGCACTGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGaa                                                    <  2:80014/148‑1 (MQ=255)
                                                                                      cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGaa                                                    >  2:281177/1‑148 (MQ=255)
                                                                                      cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGaa                                                    >  1:3217215/1‑148 (MQ=255)
                                                                                          cgcAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCg                                                <  1:1198968/148‑1 (MQ=255)
                                                                                            cAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGat                                              >  2:3432877/1‑148 (MQ=255)
                                                                                            cAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGat                                              >  2:791328/1‑148 (MQ=255)
                                                                                             aaTGGAGCGGGTTTTTTTTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGata                                             >  2:2593199/1‑148 (MQ=255)
                                                                                              ctGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGatat                                            <  1:309806/147‑1 (MQ=255)
                                                                                              aTGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGatat                                            >  2:2530075/1‑148 (MQ=255)
                                                                                              aTGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGatat                                            >  2:86355/1‑148 (MQ=255)
                                                                                               tGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATg                                           >  1:3061460/1‑148 (MQ=255)
                                                                                               tGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATg                                           >  2:2072229/1‑148 (MQ=255)
                                                                                                ggCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGc                                          <  1:2026003/148‑1 (MQ=255)
                                                                                                  cgcgGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCtt                                        <  1:3510557/148‑1 (MQ=255)
                                                                                                   gcgGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCttt                                       <  2:3206105/148‑1 (MQ=255)
                                                                                                   gcgGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCttt                                       <  2:3228885/148‑1 (MQ=255)
                                                                                                    cgGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCtttt                                      <  2:903942/148‑1 (MQ=255)
                                                                                                     gggTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTa                                     <  2:3273671/148‑1 (MQ=255)
                                                                                                      ggTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTag                                    <  1:1871638/148‑1 (MQ=255)
                                                                                                       gTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTaga                                   <  2:1772820/148‑1 (MQ=255)
                                                                                                          ttttGTTTGACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGtt                                <  1:1876605/148‑1 (MQ=255)
                                                                                                           tttgtttgACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTa                               <  2:2358561/148‑1 (MQ=255)
                                                                                                                ttgACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGa                          >  2:2467442/1‑148 (MQ=255)
                                                                                                                 tgACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGAt                         <  2:379339/148‑1 (MQ=255)
                                                                                                                 tgACTGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGAt                         <  1:609754/148‑1 (MQ=255)
                                                                                                                     tGCGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACaa                     >  2:482965/1‑148 (MQ=255)
                                                                                                                       cGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACaaaa                   <  2:3382250/148‑1 (MQ=255)
                                                                                                                       cGTGCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACaaaa                   >  2:1385222/1‑148 (MQ=255)
                                                                                                                          gCTGGCTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACg                >  2:1252626/1‑148 (MQ=255)
                                                                                                                               cTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGt           >  1:1535990/1‑148 (MQ=255)
                                                                                                                               cTTAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGt           <  2:1815466/148‑1 (MQ=255)
                                                                                                                                 tAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACaaaa                   >  2:1120655/1‑138 (MQ=255)
                                                                                                                                 tAATGCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACaaaa                   <  1:1120655/138‑1 (MQ=255)
                                                                                                                                     gCTGGATGCTGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAAccc     <  2:690543/148‑1 (MQ=255)
                                                                                                                                        ggATGCAGCTCACTCGTCTACCGCGCAAAGAAGATGCGATTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGACATTAAATCAGCTCCAGATGAATGCGATATGCTTATAGAGTTACAGGATACAAAAACGGATGTCAACCCTGa  >  2:1703749/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                             
CAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGA  >  CP009273/79981‑80264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑