Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 730,891 G→T 100% pseudogene (1303/1521 nt) rhsO → pseudogene, Rhs family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273730,8910GT97.8% 863.0 / 8.0 272pseudogene (1303/1521 nt)rhsOpseudogene, Rhs family protein
Reads supporting (aligned to +/- strand):  ref base G (5/1);  new base T (158/108);  total (163/109)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 285 total aligned reads displayed.

ACAATTAAGACTGCCATATCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATGATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATTAATAAACCCGATGGGCCAGCAA  >  CP009273/730746‑731035
                                                                                                                                                 |                                                                                                                                                
aCAATTAAGACTGCCATATCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGAATAGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGgattat                                                                                                                                                >  2:1033997/1‑148 (MQ=255)
 cAATTAAGACTGCCATATCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTCAAACCAAATCTGACTTGTACGCCACAGGattatt                                                                                                                                               >  2:2072701/1‑148 (MQ=255)
 cAATTAAGACTGCCATATCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGattatt                                                                                                                                               >  2:1837252/1‑148 (MQ=255)
  aaTTAAGACTGCCATATCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTc                                                                                                                                              <  2:714019/148‑1 (MQ=255)
   aTTAAGACTGCCATATCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTcc                                                                                                                                             <  1:3072859/148‑1 (MQ=255)
       aGACTGCCATATCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGt                                                                                                                                         <  1:1497926/148‑1 (MQ=255)
        gACTGCCATATCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTa                                                                                                                                        >  1:2407167/1‑148 (MQ=255)
           tGCCATATCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATcc                                                                                                                                     <  1:2022656/148‑1 (MQ=255)
            gCCATATCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCa                                                                                                                                    >  2:2798233/1‑148 (MQ=255)
              cATATCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAgg                                                                                                                                  >  1:2048956/1‑148 (MQ=255)
                tatCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGgga                                                                                                                                <  2:893889/148‑1 (MQ=255)
                 atCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGggat                                                                                                                               >  2:178719/1‑148 (MQ=255)
                   ccTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACGGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGggatgg                                                                                                                             <  2:179802/148‑1 (MQ=255)
                    cTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGggatgga                                                                                                                            <  2:96005/148‑1 (MQ=255)
                      aTTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATa                                                                                                                          >  1:445185/1‑148 (MQ=255)
                        tGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACa                                                                                                                        >  2:1452108/1‑148 (MQ=255)
                          aCCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACaaa                                                                                                                      <  2:662944/148‑1 (MQ=255)
                            cAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGa                                                                                                                    >  2:1615580/1‑148 (MQ=255)
                            cAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGa                                                                                                                    >  2:3355208/1‑148 (MQ=255)
                             aGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGAc                                                                                                                   >  1:3140145/1‑148 (MQ=255)
                              ggCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACa                                                                                                                  >  2:1997379/1‑148 (MQ=255)
                                 cAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCa                                                                                                               <  1:2166118/148‑1 (MQ=255)
                                 cAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCa                                                                                                               <  2:1462959/148‑1 (MQ=255)
                                   gtagtGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAg                                                                                                             >  2:1733095/1‑148 (MQ=255)
                                    tagtGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGt                                                                                                            <  1:956058/148‑1 (MQ=255)
                                    tagtGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGt                                                                                                            <  1:1209076/148‑1 (MQ=255)
                                    tagtGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGt                                                                                                            >  1:3051850/1‑148 (MQ=255)
                                     agtGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTg                                                                                                           >  1:3494045/1‑148 (MQ=255)
                                      gtGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGa                                                                                                          >  2:936636/1‑148 (MQ=255)
                                       tGCGCCTACTGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTGGTACGCCACAGGATTATTCCCAGTATCCCGGGATGGATACCAAGACAGCACGTGAt                                                                                                         >  2:3492318/1‑148 (MQ=255)
                                       tGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGAt                                                                                                         <  2:527834/148‑1 (MQ=255)
                                       tGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGAt                                                                                                         <  2:2873875/148‑1 (MQ=255)
                                       tGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGAt                                                                                                         >  1:2544460/1‑148 (MQ=255)
                                           ccTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTAcc                                                                                                     >  1:3371544/1‑148 (MQ=255)
                                                      cAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACa                                                                                          >  2:1774361/1‑148 (MQ=255)
                                                         aaCATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAAt                                                                                       <  1:2844155/148‑1 (MQ=255)
                                                         aaCATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAAt                                                                                       >  1:2700741/1‑148 (MQ=255)
                                                           cATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTg                                                                                     >  1:3008572/1‑148 (MQ=255)
                                                           cATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTg                                                                                     <  1:2871317/148‑1 (MQ=255)
                                                           cATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTg                                                                                     >  2:2340770/1‑148 (MQ=255)
                                                             ttcttcAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGc                                                                                   >  1:2455299/1‑148 (MQ=255)
                                                               cttcAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCca                                                                                 >  1:63513/1‑148 (MQ=255)
                                                                 tcAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCcaac                                                                               >  1:3041651/1‑148 (MQ=255)
                                                                 tcAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCcaac                                                                               >  2:3458168/1‑148 (MQ=255)
                                                                   agagCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACACATTGGCcaacaa                                                                             >  2:2426629/1‑148 (MQ=255)
                                                                   agagCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCcaacaa                                                                             <  2:1922072/148‑1 (MQ=255)
                                                                   agagCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCcaacaa                                                                             <  2:1529625/148‑1 (MQ=255)
                                                                     agCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACt                                                                           >  1:780828/1‑148 (MQ=255)
                                                                     agCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACt                                                                           >  1:1958817/1‑148 (MQ=255)
                                                                     agCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATGATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACt                                                                           <  1:1125957/148‑1 (MQ=255)
                                                                        aTTAAGCCCTTACGACTACACACTTTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACATGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCaa                                                                        <  2:2110000/148‑1 (MQ=255)
                                                                         ttAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAAt                                                                       <  2:2461133/148‑1 (MQ=255)
                                                                         ttAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAAt                                                                       >  2:470540/1‑148 (MQ=255)
                                                                           aaGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTa                                                                     <  1:2682153/148‑1 (MQ=255)
                                                                             gCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTacc                                                                   >  1:1684404/1‑148 (MQ=255)
                                                                               ccTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAACTTGGCCAACAACTCAATTaccac                                                                 >  2:3318646/1‑148 (MQ=255)
                                                                                cTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCATGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTaccacc                                                                <  1:1330818/148‑1 (MQ=255)
                                                                                 ttACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTAccacca                                                               >  2:3065631/1‑148 (MQ=255)
                                                                                  tACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAg                                                              >  2:1261948/1‑148 (MQ=255)
                                                                                   aCGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAgg                                                             >  2:2698043/1‑148 (MQ=255)
                                                                                    cGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGa                                                            >  1:998330/1‑148 (MQ=255)
                                                                                     gACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGat                                                           <  2:3144062/148‑1 (MQ=255)
                                                                                     gACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGat                                                           >  1:273737/1‑148 (MQ=255)
                                                                                         acacacTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCcaacaa                                                                             >  2:1427139/1‑126 (MQ=255)
                                                                                         acacacTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCcaacaa                                                                             <  1:1427159/126‑1 (MQ=255)
                                                                                         acacacTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATAc                                                       >  1:2607622/1‑148 (MQ=255)
                                                                                         acacacTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATAc                                                       >  2:2074044/1‑148 (MQ=255)
                                                                                           acacTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACtt                                                     >  2:277639/1‑148 (MQ=255)
                                                                                            cacTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTtg                                                    >  2:787289/1‑148 (MQ=255)
                                                                                              cTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTtgtg                                                  <  1:117030/148‑1 (MQ=255)
                                                                                               tGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGc                                                 <  2:1139378/148‑1 (MQ=255)
                                                                                                     gCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAAcc                                           <  1:2259147/148‑1 (MQ=255)
                                                                                                     gCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAAcc                                           <  2:1380171/148‑1 (MQ=255)
                                                                                                     gCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAAcc                                           <  2:180994/148‑1 (MQ=255)
                                                                                                     gCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAAcc                                           >  2:747516/1‑148 (MQ=255)
                                                                                                      cAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAAccc                                          <  1:1537323/148‑1 (MQ=255)
                                                                                                       aGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCt                                         <  2:2598159/148‑1 (MQ=255)
                                                                                                         gAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCtat                                       <  2:1199811/148‑1 (MQ=255)
                                                                                                          aaGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCtatt                                      <  2:1522038/148‑1 (MQ=255)
                                                                                                           aGTGGGTAAAACCAAATCGGACTTGTACGCCACAGGATTATTCCCAGTATCCATGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCtattt                                     <  2:1973348/148‑1 (MQ=255)
                                                                                                            gTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCtattta                                    <  2:1947722/148‑1 (MQ=255)
                                                                                                               tgTAAATCCAAATCTGACTTGTACGCCACAGGATTTTTCGCAGTATCCAGGGATGGATACAAAGACAGGAAGTGAGTACGTGCGTCTGACTATTTGTCCATCCTGTCACGTACCACCTGGATATACTTGTGCAGTTCCCttttttttg                                 <  2:1954577/147‑2 (MQ=255)
                                                                                                                  aaaaCCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCa                              >  1:1892396/1‑148 (MQ=255)
                                                                                                                     aCCAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGAc                           >  1:1361655/1‑148 (MQ=255)
                                                                                                                      ccAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACa                          <  1:1659243/148‑1 (MQ=255)
                                                                                                                       cAAATCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACAt                         <  1:1812070/148‑1 (MQ=255)
                                                                                                                        aaaTCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACAtt                        >  1:416002/1‑148 (MQ=255)
                                                                                                                         aaTCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATta                       >  1:2377456/1‑148 (MQ=255)
                                                                                                                         aaTCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATta                       <  2:2003788/148‑1 (MQ=255)
                                                                                                                         aaTCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATta                       >  1:723196/1‑148 (MQ=255)
                                                                                                                          aTCTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATtaa                      >  2:3229474/1‑148 (MQ=255)
                                                                                                                            cTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATtaata                    >  1:1748323/1‑148 (MQ=255)
                                                                                                                            cTGACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATtaata                    <  1:2722378/148‑1 (MQ=255)
                                                                                                                              gACTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATTAATaaa                  <  2:1862854/148‑1 (MQ=255)
                                                                                                                               aCTTGTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATTAATAAAc                 <  2:187290/148‑1 (MQ=255)
                                                                                                                                   gTACGCCACAGGATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATTAATAAACCCGa             <  2:3380861/148‑1 (MQ=255)
                                                                                                                                             ggATTATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATTAATAAACCCGATGGGCCAGCa   <  1:1034037/148‑1 (MQ=255)
                                                                                                                                              gattatTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATTAATAAACCCGATGGGCCAGCaa  >  1:3244492/1‑148 (MQ=255)
                                                                                                                                              gattatTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATTAATAAACCCGATGGGCCAGCaa  >  1:3247730/1‑148 (MQ=255)
                                                                                                                                              gattatTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATTAATAAACCCGATGGGCCAGCaa  <  1:936636/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACAATTAAGACTGCCATATCCTATTGACCAGGCCAGTAGTGCGCCTAATGTTTTCAAAACATTCTTCAGAGCATTAAGCCCTTACGACTACACACTGTATTGCAGGAAGTGGGTAAAACCAAATCTGACTTGTACGCCACAGGATGATTCCCAGTATCCAGGGATGGATACAAAGACAGCAAGTGATTACCTGCCACAGACAAATTGGCCAACAACTCAATTACCACCAGGATATACTTGTGCAGAACCCTATTTATTCCCAGACATTAATAAACCCGATGGGCCAGCAA  >  CP009273/730746‑731035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑