BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	732,655	G→T	100%	pseudogene (375/1137 nt)	ybfL →	pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	732,655	0	G	T	99.1%	737.7 / ‑2.1	230	pseudogene (375/1137 nt)	ybfL	pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
Reads supporting (aligned to +/- strand): ref base G (2/0); new base T (114/114); total (116/114)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 230 total aligned reads displayed.

GTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGC  >  CP009273/732512‑732798

ggaTCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTc                                                                                                                                             <  2:677476/146‑1 (MQ=37)

gTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTc                                                                                                                                             <  2:1526055/148‑1 (MQ=37)

 tATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCa                                                                                                                                            <  2:786066/148‑1 (MQ=37)

   tCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATg                                                                                                                                          >  2:1672809/1‑148 (MQ=37)

   tCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATg                                                                                                                                          <  1:505754/148‑1 (MQ=37)

      tGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCa                                                                                                                                       <  1:2852055/148‑1 (MQ=37)

          tCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATCAAGACGTCATTGCAATGGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAg                                                                                                                                   <  2:2000826/148‑1 (MQ=255)

           cAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGt                                                                                                                                  >  2:2104868/1‑148 (MQ=37)

             gTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGc                                                                                                                                >  2:114341/1‑148 (MQ=37)

               ccTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGt                                                                                                                              >  2:1805458/1‑148 (MQ=37)

                  gCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTtct                                                                                                                           <  1:3422343/148‑1 (MQ=37)

                    aaaaTTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTca                                                                                                                         <  2:271595/148‑1 (MQ=37)

                     aaaTTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTcaa                                                                                                                        >  1:948533/1‑148 (MQ=37)

                      aaTTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTcaac                                                                                                                       >  1:341755/1‑148 (MQ=37)

                       aTTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTcaaca                                                                                                                      >  1:2618257/1‑148 (MQ=37)

                       aTTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTcaaca                                                                                                                      >  1:2965695/1‑148 (MQ=37)

                          tCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATg                                                                                                                   <  1:2211505/148‑1 (MQ=37)

                           cACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATACAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGc                                                                                                                  <  2:3133533/148‑1 (MQ=255)

                           cACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGc                                                                                                                  <  2:3171621/148‑1 (MQ=37)

                             cGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGcac                                                                                                                >  2:3172613/1‑148 (MQ=37)

                             cGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGcac                                                                                                                >  1:1579543/1‑148 (MQ=37)

                              gAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGcaca                                                                                                               >  2:3027264/1‑148 (MQ=37)

                               aGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAg                                                                                                              >  2:359175/1‑148 (MQ=37)

                               aGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGAACAg                                                                                                              >  2:2661480/1‑148 (MQ=255)

                                 tGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAATTCGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTc                                                                                                            <  2:323089/148‑1 (MQ=255)

                                  gCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCt                                                                                                           <  2:718585/148‑1 (MQ=37)

                                   cTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTg                                                                                                          <  2:2892029/148‑1 (MQ=37)

                                    tttATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTgg                                                                                                         <  2:2262474/148‑1 (MQ=37)

                                    tttATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTgg                                                                                                         <  2:1644196/148‑1 (MQ=37)

                                        ttaACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCAt                                                                                                     >  1:664052/1‑148 (MQ=37)

                                         taACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATc                                                                                                    >  1:3082939/1‑148 (MQ=37)

                                         taACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATc                                                                                                    >  1:3082800/1‑148 (MQ=37)

                                           aCTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCgg                                                                                                  >  2:3139367/1‑148 (MQ=37)

                                           aCTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCgg                                                                                                  >  2:1584983/1‑148 (MQ=37)

                                                   cGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCa                                                                                          <  2:1161092/148‑1 (MQ=255)

                                                     tGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAg                                                                                        <  2:2544799/148‑1 (MQ=255)

                                                     tGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAg                                                                                        <  1:1977755/148‑1 (MQ=255)

                                                       aTTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGAc                                                                                      <  1:571482/148‑1 (MQ=38)

                                                         tGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACgg                                                                                    >  2:3071395/1‑148 (MQ=37)

                                                         tGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACgg                                                                                    >  2:2176293/1‑148 (MQ=37)

                                                         tGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACgg                                                                                    <  2:2099140/148‑1 (MQ=37)

                                                         tGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACgg                                                                                    >  2:1131808/1‑148 (MQ=37)

                                                            cATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATa                                                                                 >  1:3111557/1‑148 (MQ=38)

                                                             aTTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGNAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATaa                                                                                >  1:1783468/1‑148 (MQ=255)

                                                              ttCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAg                                                                               >  1:888312/1‑148 (MQ=255)

                                                              ttCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAg                                                                               >  2:1095823/1‑148 (MQ=255)

                                                               tcatcaAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGa                                                                              >  1:77257/1‑148 (MQ=255)

                                                                  tcaAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAAt                                                                           <  2:3423180/148‑1 (MQ=37)

                                                                        gATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATg                                                                     <  2:2618290/148‑1 (MQ=37)

                                                                        gATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATg                                                                     <  2:2618257/148‑1 (MQ=37)

                                                                        gATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATg                                                                     <  1:53160/148‑1 (MQ=37)

                                                                        gATAAAGACGTCATTGCAATTGATGGAAAAACGCACCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATg                                                                     >  2:1863568/1‑148 (MQ=255)

                                                                          tAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATgag                                                                   >  1:3406921/1‑148 (MQ=37)

                                                                           aaaGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATgaga                                                                  >  2:1293946/1‑148 (MQ=37)

                                                                           aaaGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATgaga                                                                  >  2:1293246/1‑148 (MQ=37)

                                                                               aCGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATcac                                                              >  1:2022641/1‑148 (MQ=255)

                                                                                   cATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCt                                                          >  2:119877/1‑148 (MQ=255)

                                                                                   cATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCt                                                          <  1:14812/148‑1 (MQ=255)

                                                                                         aaTTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCCCAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCt                                                    >  1:1549272/1‑148 (MQ=255)

                                                                                           ttGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGa                                                  <  1:1960996/148‑1 (MQ=255)

                                                                                            tGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGaa                                                 <  1:131832/148‑1 (MQ=255)

                                                                                                ggAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAActtc                                             <  2:2189821/148‑1 (MQ=255)

                                                                                                ggAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAActtc                                             <  2:2747968/148‑1 (MQ=255)

                                                                                                 gAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAActtct                                            >  2:537135/1‑148 (MQ=255)

                                                                                                   aaaaCGCTACGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTa                                          <  2:2858140/148‑1 (MQ=255)

                                                                                                    aaaCGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTaa                                         >  1:3190148/1‑148 (MQ=255)

                                                                                                    aaaCGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTaa                                         >  2:542114/1‑148 (MQ=255)

                                                                                                     aaCGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAAc                                        >  1:1143272/1‑148 (MQ=255)

                                                                                                     aaCGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAAc                                        <  2:2519613/148‑1 (MQ=255)

                                                                                                     aaCGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAAc                                        >  2:2360478/1‑148 (MQ=255)

                                                                                                     aaCGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAAc                                        >  1:230469/1‑148 (MQ=255)

                                                                                                     aaCGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAAc                                        >  1:1143646/1‑148 (MQ=255)

                                                                                                      aCGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACa                                       <  1:2773285/148‑1 (MQ=255)

                                                                                                      aCGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACa                                       <  1:1019843/148‑1 (MQ=255)

                                                                                                         cTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAAct                                               <  2:642605/137‑1 (MQ=255)

                                                                                                         cTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGc                                    >  1:2180276/1‑148 (MQ=255)

                                                                                                           ccGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTg                                  <  2:2495763/148‑1 (MQ=255)

                                                                                                            cGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTgg                                 <  1:262619/148‑1 (MQ=255)

                                                                                                             ggCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGa                                >  2:1053115/1‑148 (MQ=255)

                                                                                                             ggCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGa                                <  1:2154065/148‑1 (MQ=255)

                                                                                                             ggCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGa                                <  1:386615/148‑1 (MQ=255)

                                                                                                              gCACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGat                               >  1:687478/1‑148 (MQ=255)

                                                                                                               cACTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGata                              <  1:307626/148‑1 (MQ=255)

                                                                                                                aCTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGatat                             <  1:3168524/148‑1 (MQ=38)

                                                                                                                aCTCTTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGatat                             <  1:2520631/148‑1 (MQ=38)

                                                                                                                 ctctTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATAtt                            >  2:1773485/1‑148 (MQ=38)

                                                                                                                 ctctTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATAtt                            >  1:2546302/1‑148 (MQ=38)

                                                                                                                   ctTATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTaa                          >  2:2328051/1‑148 (MQ=37)

                                                                                                                     tATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAg                        >  2:146401/1‑148 (MQ=37)

                                                                                                                     tATGACAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAg                        >  2:146486/1‑148 (MQ=37)

                                                                                                                         aCAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGaaa                    >  2:1916339/1‑148 (MQ=37)

                                                                                                                          cAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGaaaa                   >  2:907597/1‑148 (MQ=37)

                                                                                                                          cAAGAGTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGaaaa                   >  2:2487232/1‑148 (MQ=37)

                                                                                                                            agagTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAat                 <  2:945775/148‑1 (MQ=37)

                                                                                                                             gagTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAatc                <  1:3320751/148‑1 (MQ=37)

                                                                                                                             gagTCGCCGCAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAatc                <  2:2764847/148‑1 (MQ=37)

                                                                                                                                     gcAGGGGAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAAc        <  1:1672780/148‑1 (MQ=38)

                                                                                                                                        ggggAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGa     <  2:3161308/148‑1 (MQ=38)

                                                                                                                                          ggAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATg   <  1:2184215/148‑1 (MQ=37)

                                                                                                                                           gAGCTATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGc  >  1:854879/1‑148 (MQ=37)

GTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGATTGCCATTCATCAAATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCACTCTTATGACAAGAGTCGCCGCAGGGGAGCGATTCATGTCATTAGTGCGTTCTCAACAATGCACAGTCTGGTCATCGGGCAGATCAAGACGGATAAGAAATCCAATGAGATCACAGCTATCCCTGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGC  >  CP009273/732512‑732798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`