Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 743,797 G→T 100% R214R (CGC→CGA)  ybgO ← putative fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273743,7970GT98.5% 632.0 / 0.9 200R214R (CGC→CGA) ybgOputative fimbrial protein
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base T (111/86);  total (111/89)
Fisher's exact test for biased strand distribution p-value = 8.65e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 202 total aligned reads displayed.

TGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTG  >  CP009273/743652‑743941
                                                                                                                                                 |                                                                                                                                                
tGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAAAAACCTGTGTTTCCGGTGAAATGGAAAAATTGTCGCTGCAATCCAGCActcg                                                                                                                                                >  1:1384817/1‑148 (MQ=255)
 gCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGa                                                                                                                                               <  2:537383/148‑1 (MQ=255)
  cATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGaa                                                                                                                                              <  2:1571330/148‑1 (MQ=255)
   aTTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAAt                                                                                                                                             <  2:73219/148‑1 (MQ=255)
   aTTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAAt                                                                                                                                             <  2:73603/148‑1 (MQ=255)
    ttGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATg                                                                                                                                            <  2:3115181/148‑1 (MQ=255)
    ttGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATg                                                                                                                                            <  1:1506347/148‑1 (MQ=255)
    ttGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATg                                                                                                                                            <  2:619683/148‑1 (MQ=255)
    ttGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGAAATTTTGTGTCGACGTAAATACAGCAAATTAAAATTACAAAAATCAAACACCTGTGTTTAAGGTGAAATGGAAAAAATGACGCTGCAATCAATCACTCGAATg                                                                                                                                            >  2:2057347/1‑148 (MQ=255)
    ttGATCGTTTTGGCTCTTGGTCGTTTTAATAATGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTAACAAAATCAATCACCAGTGTTTCCGGTGAAATTGAAAAATTGAAGCTGCAATCCAGCACTCGAATg                                                                                                                                            >  1:3314931/1‑148 (MQ=255)
         cGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTc                                                                                                                                       <  1:3503161/148‑1 (MQ=255)
              tGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGAc                                                                                                                                  <  2:3450497/148‑1 (MQ=255)
              tGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAAACAATCAACTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGAc                                                                                                                                  >  1:1362258/1‑148 (MQ=255)
              tGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAAGGAAATTTACCAACATCACTCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGAc                                                                                                                                  <  2:3229460/148‑1 (MQ=255)
                 ctctTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAg                                                                                                                               >  2:1386987/1‑148 (MQ=255)
                  tctTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGt                                                                                                                              >  2:1067776/1‑148 (MQ=255)
                  tctTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGt                                                                                                                              <  1:1129930/148‑1 (MQ=255)
                  tctTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTACAGACCAGt                                                                                                                              >  2:1067728/1‑148 (MQ=255)
                      ggTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAata                                                                                                                          <  2:2408909/148‑1 (MQ=255)
                        tCGTTTTAATACTGAACGTTTTCGACAATTTTTGGCGACGGATATACAGCAAATTAAATTTACCAAAATAAATAACCTGTGTTTACGGTGAAATTGAAAAATTTAAGCTGCAAACCAGCACTCGAATGTTTTACATACCAGTAaaatt                                                                                                                        >  1:2335225/1‑147 (MQ=255)
                         cGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGa                                                                                                                       <  1:1007378/148‑1 (MQ=255)
                         cGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGa                                                                                                                       <  1:2735704/148‑1 (MQ=255)
                          gTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGat                                                                                                                      <  1:2390026/148‑1 (MQ=255)
                            tttAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGatat                                                                                                                    >  2:2824799/1‑148 (MQ=255)
                             ttAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATAtt                                                                                                                   >  1:1257503/1‑148 (MQ=255)
                             ttAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATAtt                                                                                                                   >  2:148139/1‑148 (MQ=255)
                                 tACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAg                                                                                                               >  1:2919147/1‑148 (MQ=255)
                                    tGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGtt                                                                                                            <  1:3300708/148‑1 (MQ=255)
                                     gAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGttt                                                                                                           >  1:2173425/1‑148 (MQ=255)
                                      aaCGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCACTCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGtttt                                                                                                          <  2:1126024/148‑1 (MQ=255)
                                       aCGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCTGACCAGTAATATGATATTTCAGGTTTTg                                                                                                         >  2:3192983/1‑148 (MQ=255)
                                       aCGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTg                                                                                                         >  2:250539/1‑148 (MQ=255)
                                       aCGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTg                                                                                                         <  2:2036229/148‑1 (MQ=255)
                                          ttttCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGc                                                                                                      <  1:2588603/148‑1 (MQ=255)
                                            ttCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAg                                                                                                    >  1:1873578/1‑148 (MQ=255)
                                                aCATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAgg                                                                                                <  2:2126153/148‑1 (MQ=255)
                                                   ttGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTc                                                                                             <  2:2335225/148‑1 (MQ=255)
                                                    tgtgtgGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCt                                                                                            <  2:3442676/148‑1 (MQ=255)
                                                      tgtgGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTc                                                                                          <  2:1507866/148‑1 (MQ=255)
                                                               gTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCt                                                                                 >  2:693260/1‑148 (MQ=255)
                                                               gTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCt                                                                                 >  2:3261409/1‑148 (MQ=255)
                                                                tataTCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCCGTAGGGTCTTCGTTAACGCTa                                                                                >  2:2644080/1‑148 (MQ=255)
                                                                 ctatCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAAGGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTAc                                                                               >  1:1814477/2‑148 (MQ=255)
                                                                      cAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCa                                                                          >  1:514608/1‑148 (MQ=255)
                                                                            attaaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCa                                                                    >  2:972770/1‑148 (MQ=255)
                                                                            attaaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCa                                                                    >  2:1510677/1‑148 (MQ=255)
                                                                             ttaaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCaa                                                                   <  1:933069/148‑1 (MQ=255)
                                                                             ttaaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCaa                                                                   >  1:2550599/1‑148 (MQ=255)
                                                                             ttaaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCaa                                                                   <  2:19928/148‑1 (MQ=255)
                                                                             ttaaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCaa                                                                   <  1:2143479/148‑1 (MQ=255)
                                                                              taaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCaaa                                                                  >  1:1606965/1‑148 (MQ=255)
                                                                                  tttACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGc                                                              >  1:2361489/1‑148 (MQ=255)
                                                                                  tttACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGc                                                              >  1:2432035/1‑148 (MQ=255)
                                                                                  tttACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGc                                                              <  2:876057/148‑1 (MQ=255)
                                                                                  tttACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGc                                                              <  2:2807872/148‑1 (MQ=255)
                                                                                  tttACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGc                                                              >  2:537302/1‑148 (MQ=255)
                                                                                   ttACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCa                                                             >  2:571121/1‑148 (MQ=255)
                                                                                        aaaaTCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCacgacg                                                        <  2:3500090/148‑1 (MQ=255)
                                                                                          aatcaatcACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGat                                                      >  1:693716/1‑148 (MQ=255)
                                                                                          aatcaatcACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGat                                                      >  1:2333396/1‑148 (MQ=255)
                                                                                           atcaatcaCCTGTGTTTCCTGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTAGATAACGCTACCGGCACCGTCAAATTGAACGACtata                                                     >  2:1079214/1‑148 (MQ=255)
                                                                                           atcaatcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGata                                                     >  1:312903/1‑148 (MQ=255)
                                                                                           atcaatcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGata                                                     <  1:1933843/148‑1 (MQ=255)
                                                                                           atcaatcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGata                                                     <  2:722999/148‑1 (MQ=255)
                                                                                            tcaatcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGatat                                                    <  1:1480538/148‑1 (MQ=255)
                                                                                              aatcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAg                                                  <  2:50067/148‑1 (MQ=255)
                                                                                              aatcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAg                                                  <  1:365787/148‑1 (MQ=255)
                                                                                                 caCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCg                                               >  1:2382786/1‑148 (MQ=255)
                                                                                                 caCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCg                                               >  2:2567855/1‑148 (MQ=255)
                                                                                                   ccTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCt                                             >  2:1807933/1‑148 (MQ=255)
                                                                                                    cTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTa                                            >  1:3165838/1‑148 (MQ=255)
                                                                                                      gtgtTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGAAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAg                                          >  1:205444/1‑148 (MQ=255)
                                                                                                       tgtTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGc                                         <  2:2234115/148‑1 (MQ=255)
                                                                                                         tttCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGt                                       >  2:194535/1‑148 (MQ=255)
                                                                                                         tttCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGt                                       <  2:398085/148‑1 (MQ=255)
                                                                                                          ttCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTa                                      <  1:1661345/148‑1 (MQ=255)
                                                                                                          ttCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTa                                      >  1:380000/1‑148 (MQ=255)
                                                                                                              ggTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACttt                                  >  2:1348928/1‑148 (MQ=255)
                                                                                                               gTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTg                                 <  2:2919147/148‑1 (MQ=255)
                                                                                                                tGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGa                                >  2:1653589/1‑148 (MQ=255)
                                                                                                                tGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGa                                >  1:921297/1‑148 (MQ=255)
                                                                                                                   aaTGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATaa                             >  1:1712839/1‑148 (MQ=255)
                                                                                                                    aTGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAAt                            >  2:2346626/1‑148 (MQ=255)
                                                                                                                    aTGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAAt                            >  1:3404546/1‑148 (MQ=255)
                                                                                                                     tGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATc                           <  2:78135/148‑1 (MQ=255)
                                                                                                                      ggAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCa                          >  2:3275483/1‑148 (MQ=255)
                                                                                                                      ggAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCa                          >  2:2549963/1‑148 (MQ=255)
                                                                                                                      ggAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCa                          >  1:323623/1‑148 (MQ=255)
                                                                                                                        aaaaaTTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATg                        >  2:302140/1‑148 (MQ=255)
                                                                                                                              tGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGcggcgg                  >  2:822822/1‑148 (MQ=255)
                                                                                                                              tGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGcggcgg                  >  1:3064248/1‑148 (MQ=255)
                                                                                                                                aCGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCa                >  2:263285/1‑148 (MQ=255)
                                                                                                                                    tGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTtc            >  1:580260/1‑148 (MQ=255)
                                                                                                                                     gCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTtct           >  1:1047308/1‑148 (MQ=255)
                                                                                                                                        aTCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGt        <  1:2662961/148‑1 (MQ=255)
                                                                                                                                        aTCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGt        <  1:909299/148‑1 (MQ=255)
                                                                                                                                         tCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTa       <  2:1727517/148‑1 (MQ=255)
                                                                                                                                          ccAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTAt      <  1:2492322/148‑1 (MQ=255)
                                                                                                                                           cAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTAtt     <  1:1965683/148‑1 (MQ=255)
                                                                                                                                           cAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTAtt     >  2:294185/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTG  >  CP009273/743652‑743941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑