Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 748,707 A→T 100% I406I (ATT→ATA)  gltA ← citrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273748,7070AT97.1% 646.8 / 5.8 202I406I (ATT→ATA) gltAcitrate synthase
Reads supporting (aligned to +/- strand):  ref base A (4/2);  new base T (100/96);  total (104/98)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 202 total aligned reads displayed.

TTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTAC  >  CP009273/748563‑748851
                                                                                                                                                |                                                                                                                                                
tttttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATc                                                                                                                                               >  1:3222329/1‑148 (MQ=255)
     gtgtTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCAt                                                                                                                                          <  1:426535/148‑1 (MQ=255)
     gtgtTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCAt                                                                                                                                          <  1:943273/148‑1 (MQ=255)
     gtgtTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCAt                                                                                                                                          <  1:944253/148‑1 (MQ=255)
       gtTTAGGCATATAAAAATCAACCCGCCCTATGAACGGCGGGTTAAACTATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATAc                                                                                                                                        <  2:3127848/148‑1 (MQ=255)
       gtTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATAc                                                                                                                                        >  2:1082720/1‑148 (MQ=255)
        tttAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATAcc                                                                                                                                       >  1:2171354/1‑148 (MQ=255)
            ggCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCa                                                                                                                                   >  2:1688454/1‑148 (MQ=255)
             gCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCAc                                                                                                                                  >  2:1020561/1‑148 (MQ=255)
                 ataAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACtgtg                                                                                                                              >  1:3339100/1‑148 (MQ=255)
                 ataAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACtgtg                                                                                                                              >  1:2153108/1‑148 (MQ=255)
                   aaaaaTCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCa                                                                                                                            <  2:3082545/148‑1 (MQ=255)
                     aaaTCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCAtt                                                                                                                          >  1:1146149/1‑148 (MQ=255)
                     aaaTCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCAtt                                                                                                                          <  1:958537/148‑1 (MQ=255)
                      aaTCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCAttt                                                                                                                         <  2:1226744/148‑1 (MQ=255)
                         cAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATGTCCTGTATACAGCTGACGCGGACGGGCTCTCTTCATACCGTCACTGGGCATTTCGc                                                                                                                      >  2:100224/1‑148 (MQ=255)
                           aCCCGCCATATGAACGGCGGGTTAAGATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTc                                                                                                                    <  1:1272535/148‑1 (MQ=255)
                             ccGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTACATACCGTCACTGTGCATTTCGCTCCa                                                                                                                  >  2:52301/1‑148 (MQ=255)
                              cGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAg                                                                                                                 >  2:170812/1‑148 (MQ=255)
                                ccATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTg                                                                                                               <  1:1604329/148‑1 (MQ=255)
                                 cATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTgg                                                                                                              <  1:2986533/148‑1 (MQ=255)
                                  atatGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTggg                                                                                                             >  2:3200109/1‑148 (MQ=255)
                                     tGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGa                                                                                                          <  1:2442743/148‑1 (MQ=255)
                                      gAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGAt                                                                                                         >  2:3464056/1‑148 (MQ=255)
                                          ggcggGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAg                                                                                                     <  1:1041118/148‑1 (MQ=255)
                                             gggTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCa                                                                                                  <  2:1954788/148‑1 (MQ=255)
                                               gTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAAc                                                                                                >  1:1398015/1‑148 (MQ=255)
                                               gTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAAc                                                                                                >  1:3145575/1‑148 (MQ=255)
                                                   aaaTATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTa                                                                                            >  2:419084/1‑148 (MQ=255)
                                                      tatTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGATGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGt                                                                                         >  1:1586782/1‑148 (MQ=255)
                                                      tatTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGt                                                                                         >  2:185900/1‑148 (MQ=255)
                                                                  aGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaaa                                                                             <  2:779382/148‑1 (MQ=255)
                                                                   gCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaaaa                                                                            <  1:3427294/148‑1 (MQ=255)
                                                                   gCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaaaa                                                                            <  2:1304992/148‑1 (MQ=255)
                                                                   gCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaaaa                                                                            >  2:614062/1‑148 (MQ=255)
                                                                    cAATCAACCATTAACGCTGGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAAt                                                                           >  1:2339366/1‑148 (MQ=255)
                                                                     aaTCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATg                                                                          <  2:1673284/148‑1 (MQ=255)
                                                                     aaTCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATg                                                                          <  1:1654888/148‑1 (MQ=255)
                                                                     aaTCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATg                                                                          <  1:1747786/148‑1 (MQ=255)
                                                                     aaTCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATg                                                                          <  2:3412796/148‑1 (MQ=255)
                                                                      aTCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGa                                                                         <  2:2503356/148‑1 (MQ=255)
                                                                          aCCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGt                                                                     >  1:245209/1‑148 (MQ=255)
                                                                          aCCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGt                                                                     >  2:2360821/1‑148 (MQ=255)
                                                                           ccATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTg                                                                    >  1:519949/1‑148 (MQ=255)
                                                                            cATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGa                                                                   <  2:2175764/148‑1 (MQ=255)
                                                                              ttAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAAc                                                                 <  1:2545725/148‑1 (MQ=255)
                                                                              ttAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAAc                                                                 >  1:2254359/1‑148 (MQ=255)
                                                                               tAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGACAATGACGGTGAACa                                                                >  2:2990461/1‑148 (MQ=255)
                                                                               tAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACa                                                                >  2:2882919/1‑148 (MQ=255)
                                                                               tAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACa                                                                >  2:2133316/1‑148 (MQ=255)
                                                                                aaCGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTTCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACAt                                                               <  1:555242/148‑1 (MQ=255)
                                                                                 aCGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATg                                                              >  1:1165675/1‑148 (MQ=255)
                                                                                  cGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATgg                                                             >  1:1561752/1‑148 (MQ=255)
                                                                                     ttGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAg                                                          <  1:1123401/148‑1 (MQ=255)
                                                                                        atatCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACg                                                       >  2:666134/1‑148 (MQ=255)
                                                                                        atatCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACg                                                       >  2:666059/1‑148 (MQ=255)
                                                                                          atCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCCTTGCGAAAATGACGGTGAACATGGAAGACGGa                                                     >  2:3201250/1‑148 (MQ=255)
                                                                                          atCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGa                                                     >  1:1662370/1‑148 (MQ=255)
                                                                                          atCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGa                                                     <  1:2360204/148‑1 (MQ=255)
                                                                                            cGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAAt                                                   >  1:2847432/1‑148 (MQ=255)
                                                                                            cGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAAt                                                   <  2:3048932/148‑1 (MQ=255)
                                                                                              cTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATAc                                                 <  2:2272898/148‑1 (MQ=255)
                                                                                              cTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTAGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATaa                                                 >  1:1791708/1‑147 (MQ=255)
                                                                                                    aaGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCg                                           >  1:3480589/1‑148 (MQ=255)
                                                                                                      gTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCCTTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCt                                         >  2:161085/1‑148 (MQ=255)
                                                                                                      gTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCt                                         >  2:159085/1‑148 (MQ=255)
                                                                                                      gTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCt                                         <  2:3426997/148‑1 (MQ=255)
                                                                                                       tCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCtt                                        <  2:1398015/148‑1 (MQ=255)
                                                                                                       tCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCtt                                        <  2:2308933/148‑1 (MQ=255)
                                                                                                       tCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCtt                                        <  1:819369/148‑1 (MQ=255)
                                                                                                          cgTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCa                                     >  1:771776/1‑148 (MQ=255)
                                                                                                          cgTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCa                                     <  2:2716347/148‑1 (MQ=255)
                                                                                                            tttttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAgg                                   >  2:1183023/1‑148 (MQ=255)
                                                                                                               ttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgatg                                <  1:171332/148‑1 (MQ=255)
                                                                                                                  atatCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTCCGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATa                             >  1:3143601/1‑148 (MQ=255)
                                                                                                                     tCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCa                          >  2:1419516/1‑148 (MQ=255)
                                                                                                                     tCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCa                          >  1:355463/1‑148 (MQ=255)
                                                                                                                      ccTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCag                         >  2:1499859/1‑148 (MQ=255)
                                                                                                                      ccTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCag                         >  1:2507179/1‑148 (MQ=255)
                                                                                                                      ccTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCag                         >  1:2488257/1‑148 (MQ=255)
                                                                                                                       cTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCaga                        <  1:707453/148‑1 (MQ=255)
                                                                                                                         gTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGt                      <  1:3103319/148‑1 (MQ=255)
                                                                                                                          tataCAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTa                     <  2:2505641/148‑1 (MQ=255)
                                                                                                                           ataCAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAg                    >  2:577640/1‑148 (MQ=255)
                                                                                                                            taCAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGa                   <  1:3330258/148‑1 (MQ=255)
                                                                                                                             aCAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTCCGTGCCATTGCGAAAGTGACGGTGAACATGGAAGACGGAATACCCCTCGCTTTCAGGATGATACCAGAGTAGaa                  >  2:377649/1‑148 (MQ=255)
                                                                                                                             aCAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGaa                  <  2:551433/148‑1 (MQ=255)
                                                                                                                               aGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAAt                <  2:2401393/148‑1 (MQ=255)
                                                                                                                               aGCGGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAAt                <  2:2652481/148‑1 (MQ=255)
                                                                                                                                 cTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGTAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCg              >  1:1461424/1‑148 (MQ=255)
                                                                                                                                 cTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCg              <  1:2631594/148‑1 (MQ=255)
                                                                                                                                 cTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCg              >  2:1435715/1‑148 (MQ=255)
                                                                                                                                  tGACTCGGAAGTGCTATCTTCATACCTAAACTGTGCATTTCGATCCACTGGGCGATCCATCCAACGCTAAGTGCCATTTAGAAAATGACGGTGAACATGGAAGACGGAATACACATCGCTTTCAGGATTATACCAGAGTAGAAATCGa             >  2:1542024/1‑148 (MQ=255)
                                                                                                                                  tGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGa             >  2:3284122/1‑148 (MQ=255)
                                                                                                                                    aCGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACg           <  2:2621281/148‑1 (MQ=255)
                                                                                                                                      gcgGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGtt         <  2:2123384/148‑1 (MQ=255)
                                                                                                                                       cgGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTc        <  1:1667342/148‑1 (MQ=255)
                                                                                                                                          aCGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCggg     <  2:79342/148‑1 (MQ=255)
                                                                                                                                            gggCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTa   <  1:470643/148‑1 (MQ=255)
                                                                                                                                             ggCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTAc  >  2:2769732/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTAC  >  CP009273/748563‑748851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑