Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 784,094 G→T 100% A67E (GCG→GAG)  galM ← aldose 1‑epimerase; type‑1 mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273784,0940GT97.9% 770.1 / 5.6 241A67E (GCG→GAG) galMaldose 1‑epimerase; type‑1 mutarotase
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base T (124/112);  total (126/115)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 241 total aligned reads displayed.

AGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAA  >  CP009273/783951‑784237
                                                                                                                                               |                                                                                                                                               
aGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGc                                                                                                                                             <  2:1270933/148‑1 (MQ=255)
  cGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcct                                                                                                                                           >  2:797399/1‑148 (MQ=255)
     cGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGtctgat                                                                                                                                        >  2:710804/1‑148 (MQ=255)
     cGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgat                                                                                                                                        >  2:439594/1‑148 (MQ=255)
      gTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatc                                                                                                                                       <  2:1172006/148‑1 (MQ=255)
       tttGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcc                                                                                                                                      <  1:3359922/148‑1 (MQ=255)
       tttGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcc                                                                                                                                      <  2:1903017/148‑1 (MQ=255)
         tGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcctg                                                                                                                                    <  1:1151744/148‑1 (MQ=255)
         tGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcctg                                                                                                                                    <  1:1275127/148‑1 (MQ=255)
           tCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGcctgatcctgat                                                                                                                                  <  2:2391536/148‑1 (MQ=255)
            cGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATa                                                                                                                                 <  1:416634/148‑1 (MQ=255)
              aaCCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGc                                                                                                                               >  2:90018/1‑148 (MQ=255)
                cccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGTTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCAt                                                                                                                             <  1:6479/148‑1 (MQ=255)
                cccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCAt                                                                                                                             <  1:2066055/148‑1 (MQ=255)
                 ccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCAtt                                                                                                                            <  1:3226914/148‑1 (MQ=255)
                 ccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCAtt                                                                                                                            >  1:915787/1‑148 (MQ=255)
                 ccTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCAtt                                                                                                                            <  1:2686632/148‑1 (MQ=255)
                  cTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTc                                                                                                                           <  1:2946048/148‑1 (MQ=255)
                   ttCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTcc                                                                                                                          >  2:763073/1‑148 (MQ=255)
                   ttCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTcc                                                                                                                          >  2:2232021/1‑148 (MQ=255)
                      cGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCggg                                                                                                                       >  1:810638/1‑148 (MQ=255)
                       ggCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGc                                                                                                                      <  1:2822405/148‑1 (MQ=255)
                       ggCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGc                                                                                                                      <  1:2579022/148‑1 (MQ=255)
                           cgccgTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGc                                                                                                                  >  2:1560295/1‑148 (MQ=255)
                               gTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAg                                                                                                              <  1:2866672/148‑1 (MQ=255)
                                tGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGc                                                                                                             >  2:1781777/1‑148 (MQ=255)
                                tGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTAAAACCGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGc                                                                                                             >  2:207156/1‑148 (MQ=255)
                                    gcgggTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGag                                                                                                         <  1:1666102/145‑1 (MQ=255)
                                          tAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAgcgc                                                                                                   >  2:3047840/1‑148 (MQ=255)
                                           aaCGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGcc                                                                                                  <  1:1633243/148‑1 (MQ=255)
                                            aCGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCt                                                                                                 <  1:1706242/148‑1 (MQ=255)
                                             cGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTc                                                                                                >  2:3187538/1‑148 (MQ=255)
                                                ccTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTcgcg                                                                                             >  2:679253/1‑148 (MQ=255)
                                                ccTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTcgcg                                                                                             >  1:1873508/1‑148 (MQ=255)
                                                 cTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCgg                                                                                            >  2:2894214/1‑148 (MQ=255)
                                                 cTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCgg                                                                                            >  2:1878515/1‑148 (MQ=255)
                                                    aCTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACg                                                                                         >  2:2542981/1‑148 (MQ=255)
                                                     cTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGc                                                                                        <  2:1914206/148‑1 (MQ=255)
                                                     cTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAAGAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGc                                                                                        >  1:2603797/1‑148 (MQ=255)
                                                         gCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGcc                                                                                    <  2:1596676/148‑1 (MQ=255)
                                                          cGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCa                                                                                   <  2:3113910/148‑1 (MQ=255)
                                                            aaaGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATc                                                                                 >  1:3446735/1‑148 (MQ=255)
                                                            aaaGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGAGAAGGGTGGCATCACTAACAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCCTc                                                                                 >  1:3447643/1‑148 (MQ=255)
                                                             aaGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCg                                                                                >  2:652003/1‑148 (MQ=255)
                                                              aGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCgg                                                                               >  1:1143049/1‑148 (MQ=255)
                                                               gCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCCTCGGa                                                                              >  1:2708895/1‑148 (MQ=255)
                                                                cGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGaa                                                                             <  2:1666050/148‑1 (MQ=255)
                                                                cGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGaa                                                                             <  2:3161762/148‑1 (MQ=255)
                                                                   cACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGc                                                                          <  2:1289539/148‑1 (MQ=255)
                                                                      ggTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGTCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGa                                                                       <  2:2556648/148‑1 (MQ=255)
                                                                        tttCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAAt                                                                     >  2:1127383/1‑148 (MQ=255)
                                                                        tttCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAAt                                                                     >  1:3103689/1‑148 (MQ=255)
                                                                         ttCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATa                                                                    <  2:3447643/148‑1 (MQ=255)
                                                                           cACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACg                                                                  <  1:552877/148‑1 (MQ=255)
                                                                            aCCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACgg                                                                 >  1:408279/1‑148 (MQ=255)
                                                                             ccGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACggg                                                                <  2:1046686/148‑1 (MQ=255)
                                                                                  aaaGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaa                                                           <  2:2991444/148‑1 (MQ=255)
                                                                                   aaGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaa                                                          <  2:3438202/148‑1 (MQ=255)
                                                                                    aGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaag                                                         <  1:760112/148‑1 (MQ=255)
                                                                                     ggTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCTAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagt                                                        >  1:3359334/1‑148 (MQ=255)
                                                                                       tataACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaa                                                      <  2:861865/148‑1 (MQ=255)
                                                                                        ataACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGcaagtaaa                                                     >  2:1996048/1‑148 (MQ=255)
                                                                                        ataACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaa                                                     <  2:1617698/148‑1 (MQ=255)
                                                                                        ataACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaa                                                     <  1:2791878/148‑1 (MQ=255)
                                                                                         taACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaag                                                    <  1:1781777/148‑1 (MQ=255)
                                                                                          aaCGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCCGCGCCTCGCGGACGCTGCCAGCGGAAAGCGGAATACGGGCGGaaagtaaagt                                                   >  1:307185/1‑148 (MQ=255)
                                                                                          aaCGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaagt                                                   <  2:1269756/148‑1 (MQ=255)
                                                                                          aaCGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaagt                                                   <  1:1900958/148‑1 (MQ=255)
                                                                                          aaCGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGaaagtaaagt                                                   <  2:2084801/148‑1 (MQ=255)
                                                                                               cTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCAc                                              >  2:2375604/1‑148 (MQ=255)
                                                                                                 aTTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCAccc                                            >  2:1525970/1‑148 (MQ=255)
                                                                                                 aTTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCAccc                                            >  2:1525930/1‑148 (MQ=255)
                                                                                                   tGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCa                                          >  1:396051/1‑148 (MQ=255)
                                                                                                     gCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGt                                        <  2:2336913/148‑1 (MQ=255)
                                                                                                         tACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCAt                                    >  2:2023850/1‑148 (MQ=255)
                                                                                                         tACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCAt                                    >  1:2219729/1‑148 (MQ=255)
                                                                                                         tACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCAt                                    >  1:2656887/1‑148 (MQ=255)
                                                                                                          aCGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATc                                   <  1:2344086/148‑1 (MQ=255)
                                                                                                             gTTGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGc                                >  1:1101450/1‑148 (MQ=255)
                                                                                                              ttGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCg                               <  1:90018/148‑1 (MQ=255)
                                                                                                              ttGGCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCg                               >  2:2216346/1‑148 (MQ=255)
                                                                                                                ggCATAACGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTg                             <  2:3246765/148‑1 (MQ=255)
                                                                                                                     aaCGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTAc                        >  1:2900401/1‑148 (MQ=255)
                                                                                                                       cGACCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCa                      >  1:1938547/1‑148 (MQ=255)
                                                                                                                         aCCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATc                    >  2:514239/1‑148 (MQ=255)
                                                                                                                         aCCAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATc                    >  2:1936308/1‑148 (MQ=255)
                                                                                                                          ccAATAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATcc                   >  2:3362270/1‑148 (MQ=255)
                                                                                                                            aaTAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCt                 >  2:2529315/1‑148 (MQ=255)
                                                                                                                              tAGAGGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGc               >  1:1874131/1‑148 (MQ=255)
                                                                                                                               agagGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCg              >  1:2276742/1‑148 (MQ=255)
                                                                                                                               agagGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCg              <  1:1080297/148‑1 (MQ=255)
                                                                                                                                gagGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgt             <  1:1152007/148‑1 (MQ=255)
                                                                                                                                 agGCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgtt            <  1:763073/148‑1 (MQ=255)
                                                                                                                                  ggCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgttg           <  2:3486666/148‑1 (MQ=255)
                                                                                                                                  ggCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgttg           <  2:366570/148‑1 (MQ=255)
                                                                                                                                   gCCCCCAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCgttgt          >  2:1271604/1‑148 (MQ=255)
                                                                                                                                       ccAGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACg      >  1:2157782/1‑148 (MQ=255)
                                                                                                                                         aGAAACTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAAAAAGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCa    >  1:1568184/1‑148 (MQ=255)
                                                                                                                                          gAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCaa   <  1:2542981/148‑1 (MQ=255)
                                                                                                                                           aaaCTCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCaaa  <  1:246011/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
AGCGACGTTTGTCGAACCCTTCCGGCCCGCCGTGCAGCTGGTTAACGCCCTGACTTGGCGAAAGCGTCACGGTTTCACCGTCAAAGGTATAACGGCTATTGGCGATACGGTTGGCATAACGACCAATAGAGGCCCCCAGAAACGCGGCCTGATCCTGATAGCATTCCGGGCTGGCACAGCCGAGCAGCGCCTCGCGGACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAA  >  CP009273/783951‑784237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑