Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 800,779 G→T 100% E607D (GAG→GAT)  ybhJ → putative hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273800,7790GT100.0% 679.9 / NA 206E607D (GAG→GAT) ybhJputative hydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (105/101);  total (105/101)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 211 total aligned reads displayed.

GTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGT  >  CP009273/800634‑800923
                                                                                                                                                 |                                                                                                                                                
gTGAACCCTCTCCTTATCGTACAAAGCCGTTTGGTGGGGCGGATTTTACCCTGTCTCGCCGCGAGCCCGGTTTTGTTCCCACAATTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATgt                                                                                                                                                <  1:2756848/148‑1 (MQ=255)
   aaaCCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGATTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGtt                                                                                                                                             <  1:2593808/148‑1 (MQ=255)
       ctctTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgcg                                                                                                                                         <  2:2215867/148‑1 (MQ=255)
       atcttctTAGCGTACAAATCCGTTGGGTCGGGCGGTGTTTACCGTGCCCCGCCGCGATCCCGGTTATGTTAGCACAATTACAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgcg                                                                                                                                         <  1:1251955/147‑1 (MQ=255)
        tcttctTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgcgc                                                                                                                                        >  1:860222/1‑148 (MQ=255)
        tcttctTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgcgc                                                                                                                                        >  2:2299189/1‑148 (MQ=255)
         cttcttATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgcgcg                                                                                                                                       >  2:1157566/1‑148 (MQ=255)
         cttcttATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgcgcg                                                                                                                                       >  1:2124432/1‑148 (MQ=255)
           tcttATCGTTAAAAGCCGTTTGGTGTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCa                                                                                                                                     <  1:1153083/148‑1 (MQ=255)
            cttATCGTTCAAATCGGTTTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCGGTTTATGTTAGCAGAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCAt                                                                                                                                    <  2:3278629/148‑1 (MQ=255)
              tATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTa                                                                                                                                  <  2:2006679/148‑1 (MQ=255)
              tATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTa                                                                                                                                  >  2:589796/1‑148 (MQ=255)
              tATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTa                                                                                                                                  <  2:216138/148‑1 (MQ=255)
               actgtacAAATCCGATTGGTCTGGGGTAGTTTACCCGGTCTCGCCGCGATCCCGGTTTTGTTAGCATATGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTaa                                                                                                                                 <  2:937631/142‑1 (MQ=255)
               aTCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTaa                                                                                                                                 >  1:678320/1‑148 (MQ=255)
                tcgtaaaaaTCCTTTTGGTCGGGGGTTGTTTACCGTGGCGCGCCGCGATCCGGGTTTTGTTTGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAg                                                                                                                                <  1:2984879/142‑1 (MQ=255)
                tCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAg                                                                                                                                >  1:1001832/1‑148 (MQ=255)
                tCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAg                                                                                                                                >  1:999956/1‑148 (MQ=255)
                  gTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCa                                                                                                                              <  2:2346986/148‑1 (MQ=255)
                   ttCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAg                                                                                                                             >  1:1871974/1‑148 (MQ=255)
                   ttCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAg                                                                                                                             <  2:1904771/148‑1 (MQ=255)
                    tCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGa                                                                                                                            <  2:2772015/148‑1 (MQ=255)
                    tCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGa                                                                                                                            >  2:1867791/1‑148 (MQ=255)
                    tCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGa                                                                                                                            >  2:226478/1‑148 (MQ=255)
                     cAAATCCTATTGGGCGGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTTTGTTAGCATAATTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGAt                                                                                                                           <  1:2458027/148‑1 (MQ=255)
                       aaTCCGATTGGTCTGGCGTAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTg                                                                                                                         <  2:1114557/148‑1 (MQ=255)
                       aaTCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATTTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTg                                                                                                                         <  2:3382753/148‑1 (MQ=255)
                       aaTCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCATAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTg                                                                                                                         <  2:3383052/148‑1 (MQ=255)
                             tttGGTCTGGCGGAGTTTACCCTGTCTCGCCGCTATCCCGTTTTTGTTAGCATAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTc                                                                                                                   <  1:2926955/147‑1 (MQ=255)
                             aTTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTc                                                                                                                   >  1:1386278/1‑148 (MQ=255)
                             aTTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTc                                                                                                                   >  1:733300/1‑148 (MQ=255)
                              ttGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCa                                                                                                                  >  2:2586202/1‑148 (MQ=255)
                               tGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAg                                                                                                                 >  2:694724/1‑148 (MQ=255)
                                     ggCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCat                                                                                                           >  2:319263/1‑148 (MQ=255)
                                     ggCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCat                                                                                                           >  1:1700678/1‑148 (MQ=255)
                                     ggCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCat                                                                                                           >  1:749812/1‑148 (MQ=255)
                                      gCGGATTTTACCCTGTCTCGCCGCGATCCCGGTTATTTTAGAAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCata                                                                                                          <  1:884073/148‑1 (MQ=255)
                                       cGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCatat                                                                                                         >  1:400435/1‑148 (MQ=255)
                                          aGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGa                                                                                                      >  1:3176443/1‑148 (MQ=255)
                                            tttACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATc                                                                                                    >  2:1940716/1‑148 (MQ=255)
                                                  cTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGc                                                                                              >  1:469800/1‑148 (MQ=255)
                                                    gTCTCGCCGCGATCCCGTTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCaa                                                                                            <  2:1629700/148‑1 (MQ=255)
                                                    gTCGCGCCGCGCTCCCGGTTATGTTAGCACAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCaa                                                                                            <  1:2401035/148‑1 (MQ=255)
                                                     tctcGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACCGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCaaa                                                                                           >  2:1805806/1‑148 (MQ=255)
                                                     tctcGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCaaa                                                                                           >  2:1805950/1‑148 (MQ=255)
                                                        cgccgcGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTg                                                                                        >  1:22942/1‑148 (MQ=255)
                                                             cgATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAAtt                                                                                   >  1:1940834/1‑148 (MQ=255)
                                                             cgATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGATTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGaaaat                                                                                   >  2:2941584/1‑146 (MQ=255)
                                                              taTCCGGTTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTg                                                                                  <  1:2202870/147‑1 (MQ=255)
                                                              gATCCCGGTTATTTTAGCATAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTg                                                                                  <  1:2202947/148‑1 (MQ=255)
                                                               aTCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTgg                                                                                 <  2:2826713/148‑1 (MQ=255)
                                                               aTCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTgg                                                                                 >  2:2430206/1‑148 (MQ=255)
                                                                    ggTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgcagca                                                                            >  1:955198/1‑148 (MQ=255)
                                                                     gTTATTTTAGCAGAATTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCAt                                                                           <  1:1267109/148‑1 (MQ=255)
                                                                      ttATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATg                                                                          >  1:1686910/1‑148 (MQ=255)
                                                                        aTGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGt                                                                        >  2:170065/1‑148 (MQ=255)
                                                                          gTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCt                                                                      >  2:3425665/1‑148 (MQ=255)
                                                                          gTTAGAACAAGAAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCt                                                                      <  1:3300639/148‑1 (MQ=255)
                                                                           ttAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTa                                                                     >  1:306592/1‑148 (MQ=255)
                                                                           ttAGAAGAAGTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTa                                                                     <  1:2084371/148‑1 (MQ=255)
                                                                            tAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTAt                                                                    >  2:2974272/1‑148 (MQ=255)
                                                                               cAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCg                                                                 >  2:776395/1‑148 (MQ=255)
                                                                                 taattAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGt                                                               <  1:888101/144‑1 (MQ=255)
                                                                                 gAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGt                                                               <  1:111396/148‑1 (MQ=255)
                                                                                 gAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGt                                                               >  2:1436862/1‑148 (MQ=255)
                                                                                   aGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGa                                                             >  2:7062/1‑148 (MQ=255)
                                                                                    ttAAAGCGACTGCTGAGCTGGAAAATCAGCGTCGGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGaa                                                            <  2:2666984/147‑1 (MQ=255)
                                                                                     tAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGTAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGaaa                                                           >  1:3397873/1‑148 (MQ=255)
                                                                                     tAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGaaa                                                           >  1:3012085/1‑148 (MQ=255)
                                                                                      aacgCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAAc                                                          <  1:3319209/145‑1 (MQ=255)
                                                                                       aaGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAAcc                                                         >  1:2726945/1‑148 (MQ=255)
                                                                                        aGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCa                                                        <  1:3383066/148‑1 (MQ=255)
                                                                                            aCTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCg                                                    >  1:517385/1‑148 (MQ=255)
                                                                                                 tgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGt                                               <  2:1249224/148‑1 (MQ=255)
                                                                                                 tgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGt                                               <  2:1198009/148‑1 (MQ=255)
                                                                                                     cTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTg                                           >  2:3232280/1‑148 (MQ=255)
                                                                                                       ggAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTgcg                                         >  2:1669556/1‑148 (MQ=255)
                                                                                                         aaaaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGcgcg                                       <  1:3414934/148‑1 (MQ=255)
                                                                                                          aaaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGt                                      >  1:3352249/1‑148 (MQ=255)
                                                                                                              cAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAAc                                  >  2:3084727/1‑148 (MQ=255)
                                                                                                               aGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACa                                 >  1:2222771/1‑148 (MQ=255)
                                                                                                                 cGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAgg                               <  1:3425665/148‑1 (MQ=255)
                                                                                                                     tGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAggcggc                           <  1:3135836/148‑1 (MQ=255)
                                                                                                                      ggCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGgcggcg                          <  2:2461594/148‑1 (MQ=255)
                                                                                                                        cGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAg                        <  2:2802790/148‑1 (MQ=255)
                                                                                                                         ggggAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGc                       <  2:785471/148‑1 (MQ=255)
                                                                                                                          gggACTGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCt                      >  1:1226964/1‑148 (MQ=255)
                                                                                                                           gtaaTGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTg                     <  2:964039/146‑1 (MQ=255)
                                                                                                                               tGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAg                 >  1:1213366/1‑148 (MQ=255)
                                                                                                                                 tCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCg               <  2:140497/148‑1 (MQ=255)
                                                                                                                                  cAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCgt              >  1:2518025/1‑148 (MQ=255)
                                                                                                                                     cGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGtgtg           >  2:563523/1‑148 (MQ=255)
                                                                                                                                        tcTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGAGTGAACAGGCGGCGAGCTGCCAGCGTGTGAtt        <  1:1802417/147‑1 (MQ=255)
                                                                                                                                        gCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAtt        <  1:694724/148‑1 (MQ=255)
                                                                                                                                            aCAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCg    >  2:3258249/1‑148 (MQ=255)
                                                                                                                                             cAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCgg   >  1:1315869/1‑148 (MQ=255)
                                                                                                                                             cAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCgg   >  2:1379111/1‑148 (MQ=255)
                                                                                                                                             cAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCgg   <  2:1572956/148‑1 (MQ=255)
                                                                                                                                              agatGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGt  >  1:2472068/1‑148 (MQ=255)
                                                                                                                                              agatGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGt  <  1:696012/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGT  >  CP009273/800634‑800923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑