Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 829,009 G→T 100% D162Y (GAT→TAT)  dinG → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273829,0090GT98.0% 628.9 / 3.5 199D162Y (GAT→TAT) dinGATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (82/113);  total (83/116)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 199 total aligned reads displayed.

GGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCT  >  CP009273/828865‑829155
                                                                                                                                                |                                                                                                                                                  
gggcgtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTAtc                                                                                                                                                 <  1:717140/148‑1 (MQ=255)
gggcgtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTATTTACGACGAACAGACGCCGAACAATCAGGAAGAGCAAAAAAGTTGTGCGAAGCAGAAGGGCTAtc                                                                                                                                                 >  1:917864/1‑148 (MQ=255)
   cgtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCg                                                                                                                                              <  1:2126736/148‑1 (MQ=255)
    gtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGa                                                                                                                                             <  2:2679107/148‑1 (MQ=255)
      gggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATa                                                                                                                                           >  2:2286518/1‑148 (MQ=255)
      gggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATa                                                                                                                                           <  1:2871987/148‑1 (MQ=255)
       ggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATAc                                                                                                                                          <  2:631919/148‑1 (MQ=255)
       ggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATAc                                                                                                                                          <  2:3310555/148‑1 (MQ=255)
          gcTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTta                                                                                                                                       <  1:3052238/148‑1 (MQ=255)
           cTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTtat                                                                                                                                      >  2:663401/1‑148 (MQ=255)
             aCGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATaa                                                                                                                                    >  2:2879748/1‑148 (MQ=255)
              cGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATaaa                                                                                                                                   <  2:3137269/148‑1 (MQ=255)
              cGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATaaa                                                                                                                                   >  2:456892/1‑148 (MQ=255)
               gTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAAt                                                                                                                                  <  2:3024970/148‑1 (MQ=255)
               gTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAAt                                                                                                                                  <  2:3262684/148‑1 (MQ=255)
                tttGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATg                                                                                                                                 >  1:1749659/1‑148 (MQ=255)
                 ttGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATgg                                                                                                                                >  2:1458550/1‑148 (MQ=255)
                  tGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATggg                                                                                                                               <  1:1381098/148‑1 (MQ=255)
                     ccGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATg                                                                                                                            <  2:1975339/148‑1 (MQ=255)
                     ccGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATg                                                                                                                            >  2:518902/1‑148 (MQ=255)
                     ccGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATg                                                                                                                            <  2:1270430/148‑1 (MQ=255)
                          tAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTg                                                                                                                       <  1:1340648/148‑1 (MQ=255)
                            aTCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTgcg                                                                                                                     <  2:2653897/148‑1 (MQ=255)
                               tGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGa                                                                                                                  <  1:1312637/148‑1 (MQ=255)
                                gACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGAt                                                                                                                 <  1:3481245/148‑1 (MQ=255)
                                   ggCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCAt                                                                                                              <  1:1904610/148‑1 (MQ=255)
                                   ggCGCTCGCCAGTACAGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCAt                                                                                                              <  2:1656689/148‑1 (MQ=255)
                                     cgcTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATAc                                                                                                            >  1:162562/1‑148 (MQ=255)
                                        tCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGa                                                                                                         <  2:3178142/148‑1 (MQ=255)
                                          gCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGata                                                                                                       <  1:1675850/148‑1 (MQ=255)
                                              gTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGc                                                                                                   <  1:1687171/148‑1 (MQ=255)
                                              gTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGc                                                                                                   <  1:2514995/148‑1 (MQ=255)
                                               tACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAAAGTTGTGCGAAGCTGAAGGGCTATCTCGATACGTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCt                                                                                                  >  1:451312/1‑148 (MQ=255)
                                               tACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAAAGTTGTGCGAAGCGGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCt                                                                                                  >  1:3323205/1‑148 (MQ=255)
                                               tACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACAGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCt                                                                                                  >  1:1962090/1‑148 (MQ=255)
                                                   gAACCCACGCAACAGGATCTGCTGGCGTGTATTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATTGGATGGTCTGCGCGATCAAACTGATATCGCTATAg                                                                                              >  2:2379326/1‑148 (MQ=255)
                                                    aaCCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGa                                                                                             <  2:2050135/148‑1 (MQ=255)
                                                     aCCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGAt                                                                                            >  1:813020/1‑148 (MQ=255)
                                                      cccACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATg                                                                                           <  1:2800400/148‑1 (MQ=255)
                                                      cccACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATg                                                                                           <  1:1653749/148‑1 (MQ=255)
                                                       ccACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGa                                                                                          <  1:3380053/148‑1 (MQ=255)
                                                       ccACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGa                                                                                          <  1:1444446/148‑1 (MQ=255)
                                                        cACGCAACAGGATCTGCTGGCGTTTCTTGACGACTAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGAc                                                                                         <  2:10493/148‑1 (MQ=255)
                                                         aCGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACg                                                                                        <  1:3146205/148‑1 (MQ=255)
                                                          cGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGa                                                                                       >  2:1128801/1‑148 (MQ=255)
                                                              aCAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGAtctc                                                                                   <  1:2097500/148‑1 (MQ=255)
                                                               cAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATctct                                                                                  >  1:1448654/1‑148 (MQ=255)
                                                                 ggATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTgg                                                                                <  1:3259327/148‑1 (MQ=255)
                                                                  gATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCGATAGATGACGATCTCTGGc                                                                               >  2:1026946/1‑148 (MQ=255)
                                                                   aTCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGcg                                                                              >  2:2126885/1‑148 (MQ=255)
                                                                   aTCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGcg                                                                              >  2:3435386/1‑148 (MQ=255)
                                                                    tCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGcgt                                                                             <  1:149650/148‑1 (MQ=255)
                                                                         tgGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATACATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGttt                                                                        <  1:2410847/148‑1 (MQ=255)
                                                                         tgGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGttt                                                                        <  2:234348/148‑1 (MQ=255)
                                                                          ggCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTa                                                                       <  2:1654502/148‑1 (MQ=255)
                                                                          ggCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTa                                                                       <  2:977227/148‑1 (MQ=255)
                                                                           gCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTaa                                                                      >  2:2401527/1‑148 (MQ=255)
                                                                            cGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAg                                                                     <  1:826367/148‑1 (MQ=255)
                                                                              tttCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTa                                                                   <  2:2635260/148‑1 (MQ=255)
                                                                               ttCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTAc                                                                  >  2:2213965/1‑148 (MQ=255)
                                                                               ttCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTAc                                                                  >  1:1370275/1‑148 (MQ=255)
                                                                               ttCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTAc                                                                  >  1:1465597/1‑148 (MQ=255)
                                                                                tCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTAcc                                                                 >  2:3501536/1‑148 (MQ=255)
                                                                                  ttGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGa                                                               <  1:2725271/148‑1 (MQ=255)
                                                                                     acgacgAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACaa                                                            <  1:103870/148‑1 (MQ=255)
                                                                                     acgacgAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACaa                                                            <  2:2688854/148‑1 (MQ=255)
                                                                                        acgaACTGACGCCGAACAGTCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGc                                                         >  2:2198213/1‑148 (MQ=255)
                                                                                        acgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGc                                                         >  1:2304600/1‑148 (MQ=255)
                                                                                        acgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGc                                                         <  2:847283/148‑1 (MQ=255)
                                                                                            aCTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGc                                                     >  2:1149418/1‑148 (MQ=255)
                                                                                                aCGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGcc                                                 <  1:3106924/148‑1 (MQ=255)
                                                                                                       aCAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCg                                          >  1:3030915/1‑148 (MQ=255)
                                                                                                           tCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAt                                      >  2:1530755/1‑148 (MQ=255)
                                                                                                            cAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAtt                                     <  1:1209742/148‑1 (MQ=255)
                                                                                                            cAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCGGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAtt                                     <  2:2409428/148‑1 (MQ=255)
                                                                                                             aGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCCGCTGCCTCAACCGCCATTg                                    >  2:2013146/1‑148 (MQ=255)
                                                                                                             aGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTg                                    <  2:1352156/148‑1 (MQ=255)
                                                                                                               gAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCCATTGtt                                  >  2:3037264/1‑148 (MQ=255)
                                                                                                                aaGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTta                                 <  1:708147/148‑1 (MQ=255)
                                                                                                                 agagCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtac                                >  2:2666568/1‑148 (MQ=255)
                                                                                                                 agagCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtac                                <  1:2668278/148‑1 (MQ=255)
                                                                                                                  gagCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAGTGTtact                               >  1:2867599/1‑148 (MQ=255)
                                                                                                                     cAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTactact                            >  1:2740661/1‑148 (MQ=255)
                                                                                                                     cAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTactact                            <  1:476121/148‑1 (MQ=255)
                                                                                                                         aaCGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCg                        >  2:2096964/1‑148 (MQ=255)
                                                                                                                          aCGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGt                       <  1:3501536/148‑1 (MQ=255)
                                                                                                                          aCGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGt                       >  1:498940/1‑148 (MQ=255)
                                                                                                                           cGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTg                      >  1:214534/1‑148 (MQ=255)
                                                                                                                                    aaGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGt             <  1:2038197/148‑1 (MQ=255)
                                                                                                                                     aGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATTCCCGtt            >  2:1717501/1‑148 (MQ=255)
                                                                                                                                      gCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGttt           >  2:260417/1‑148 (MQ=255)
                                                                                                                                       cTGCAGGGCTATCTCGATACTGATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGtttt          <  2:3512403/148‑1 (MQ=255)
                                                                                                                                       cTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGtttt          <  2:1558695/148‑1 (MQ=255)
                                                                                                                                       cTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGtttt          <  2:2649425/148‑1 (MQ=255)
                                                                                                                                       cTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTGTAAGTAACGACAAAGCCAGCTGCCTCAACCCCAATTGTTACTACTATCGTGAATGCCCGtttt          >  1:1556881/1‑148 (MQ=255)
                                                                                                                                          aaGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTg       <  2:2027307/148‑1 (MQ=255)
                                                                                                                                          aaGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTAATATCGTGAATGCCCGTTTTTTg       >  2:908027/1‑148 (MQ=255)
                                                                                                                                           aGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGt      >  1:1158848/1‑148 (MQ=255)
                                                                                                                                            gggCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAATGTTACTACTATCGTGAATGCCCGTTTTTTGTc     >  2:3078983/1‑148 (MQ=255)
                                                                                                                                               cGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCt  >  1:2318979/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCT  >  CP009273/828865‑829155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑