Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 837,132 C→T 100% intergenic (‑145/+120) fiu ← / ← mcbA catecholate siderophore receptor Fiu/colanic acid mucoidy stimulation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273837,1320CT98.6% 685.6 / 0.2 210intergenic (‑145/+120)fiu/mcbAcatecholate siderophore receptor Fiu/colanic acid mucoidy stimulation protein
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base T (89/118);  total (92/118)
Fisher's exact test for biased strand distribution p-value = 8.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 213 total aligned reads displayed.

TTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCAGTACCG  >  CP009273/836987‑837276
                                                                                                                                                 |                                                                                                                                                
ttttGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTa                                                                                                                                                >  2:1755836/1‑148 (MQ=255)
 tttGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTAt                                                                                                                                               <  1:2303074/148‑1 (MQ=255)
      aGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCAccc                                                                                                                                          <  1:1533126/148‑1 (MQ=255)
       ggTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCa                                                                                                                                         <  2:2477576/148‑1 (MQ=255)
            cTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTgcg                                                                                                                                    >  1:1660371/1‑148 (MQ=255)
            cTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCAACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTgcg                                                                                                                                    <  1:2779100/148‑1 (MQ=255)
              ttttCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGcgcg                                                                                                                                  >  2:2972586/1‑148 (MQ=255)
                ttCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGaa                                                                                                                                >  2:2670740/1‑148 (MQ=255)
                   ttATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGaaaaa                                                                                                                             <  2:196187/148‑1 (MQ=255)
                       atGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGaaa                                                                                                                         >  2:2297955/1‑148 (MQ=255)
                       atGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGaaa                                                                                                                         <  1:1292462/148‑1 (MQ=255)
                        tGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAAt                                                                                                                        <  2:2729519/148‑1 (MQ=255)
                         gTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATa                                                                                                                       >  1:443538/1‑148 (MQ=255)
                         gTTGAAATTTTCACTGCCACGAGAAAGACTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATa                                                                                                                       >  1:445332/1‑148 (MQ=255)
                           tGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACa                                                                                                                     <  2:2687841/148‑1 (MQ=255)
                               aTTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACattta                                                                                                                 <  2:135918/148‑1 (MQ=255)
                               aTTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACattta                                                                                                                 <  2:847111/148‑1 (MQ=255)
                               aTTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACattta                                                                                                                 <  1:771239/148‑1 (MQ=255)
                                 tttCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACatttatt                                                                                                               >  1:2623245/1‑148 (MQ=255)
                                      cTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACAt                                                                                                          >  1:232511/1‑148 (MQ=255)
                                        gCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACAtttattta                                                                                                             >  2:1008070/1‑143 (MQ=255)
                                        gCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACAtttattta                                                                                                             <  1:1008070/143‑1 (MQ=255)
                                         ccACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGa                                                                                                       >  2:3486111/1‑148 (MQ=255)
                                         ccACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGa                                                                                                       >  2:1038651/1‑148 (MQ=255)
                                         ccACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGa                                                                                                       >  1:2675260/1‑148 (MQ=255)
                                          cACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAg                                                                                                      >  1:904623/1‑148 (MQ=255)
                                            cGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGtt                                                                                                    <  2:2188134/148‑1 (MQ=255)
                                               gaAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCat                                                                                                 <  2:322949/148‑1 (MQ=255)
                                                aaaGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCata                                                                                                <  2:1226659/148‑1 (MQ=255)
                                                aaaGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCata                                                                                                >  2:2327343/1‑148 (MQ=255)
                                                    ccTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTg                                                                                            >  2:2706764/1‑148 (MQ=255)
                                                      tctctGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGAt                                                                                          >  2:3357538/1‑148 (MQ=255)
                                                      tctctGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGAt                                                                                          >  1:3426047/1‑148 (MQ=255)
                                                      tctctGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGAt                                                                                          >  1:427553/1‑148 (MQ=255)
                                                      tctctGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGAt                                                                                          >  1:428102/1‑148 (MQ=255)
                                                      tctctGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGAt                                                                                          <  2:1980612/148‑1 (MQ=255)
                                                       ctctGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATg                                                                                         >  1:127728/1‑148 (MQ=255)
                                                         ctGCGGATGCGATGCGACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGtt                                                                                       <  2:445393/148‑1 (MQ=255)
                                                          tGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGttt                                                                                      <  1:342286/148‑1 (MQ=255)
                                                            cGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGttttt                                                                                    <  2:886300/148‑1 (MQ=255)
                                                             ggATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGtttttt                                                                                   <  1:1540886/148‑1 (MQ=255)
                                                             ggATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGtttttt                                                                                   <  1:2920395/148‑1 (MQ=255)
                                                              gatgcgatgcCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTg                                                                                  <  1:2169993/148‑1 (MQ=255)
                                                               atgcgatgcCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGa                                                                                 <  2:2273886/148‑1 (MQ=255)
                                                                 gcgatgcCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCTAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGAtt                                                                               <  2:3471958/148‑1 (MQ=255)
                                                                 gcgatgcCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGAtt                                                                               <  2:527901/148‑1 (MQ=255)
                                                                    atgcCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACa                                                                            <  1:3343393/148‑1 (MQ=255)
                                                                     tgcCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACaa                                                                           >  1:3208908/1‑148 (MQ=255)
                                                                         aCTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACa                                                                       <  2:1087423/148‑1 (MQ=255)
                                                                          cTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAg                                                                      >  2:1111287/1‑148 (MQ=255)
                                                                           tGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGa                                                                     >  1:1977944/1‑148 (MQ=255)
                                                                             ttAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACg                                                                   >  2:62452/1‑148 (MQ=255)
                                                                                 tCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGaaaa                                                               <  2:2198460/148‑1 (MQ=255)
                                                                                 tCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGaaaa                                                               <  2:2270602/148‑1 (MQ=255)
                                                                                 tCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCAGATTTGATGTTTTTTGATTACAAAACAGACGaaaa                                                               >  2:2773845/1‑148 (MQ=255)
                                                                                     gTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGc                                                           >  1:3385748/1‑148 (MQ=255)
                                                                                     gTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGc                                                           <  2:1020563/148‑1 (MQ=255)
                                                                                      tGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCa                                                          >  1:699827/1‑148 (MQ=255)
                                                                                       gATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCAt                                                         <  1:1939418/148‑1 (MQ=255)
                                                                                       gATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCAt                                                         <  1:2970540/148‑1 (MQ=255)
                                                                                        aTAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATc                                                        <  1:2165470/148‑1 (MQ=255)
                                                                                         tAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATcc                                                       >  2:1440292/1‑148 (MQ=255)
                                                                                         tAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATcc                                                       >  2:3244806/1‑148 (MQ=255)
                                                                                           gTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCag                                                     <  1:600096/148‑1 (MQ=255)
                                                                                           gTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCag                                                     <  1:599997/148‑1 (MQ=255)
                                                                                              cTCGTGAAAGTGATAATAATTTTGATAAGCATTATCTCTTGTAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAgg                                                  <  2:3035237/148‑1 (MQ=255)
                                                                                                 gTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGa                                               <  2:2925454/148‑1 (MQ=255)
                                                                                                     aaGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGc                                           <  2:510321/148‑1 (MQ=255)
                                                                                                      aGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCa                                          >  1:1670288/1‑148 (MQ=255)
                                                                                                      aGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCa                                          >  2:2975813/1‑148 (MQ=255)
                                                                                                        tGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGt                                        >  2:989666/1‑148 (MQ=255)
                                                                                                        tGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGt                                        >  1:2221954/1‑148 (MQ=255)
                                                                                                        tGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGt                                        <  2:1624986/148‑1 (MQ=255)
                                                                                                         gATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGtt                                       >  1:374689/1‑148 (MQ=255)
                                                                                                           taataaTTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCTAATGCAGTTAc                                     >  1:3271145/1‑148 (MQ=255)
                                                                                                           taataaTTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTAc                                     >  1:984245/1‑148 (MQ=255)
                                                                                                             ataatTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGt                                   <  1:546632/148‑1 (MQ=255)
                                                                                                             ataatTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGt                                   <  1:763500/148‑1 (MQ=255)
                                                                                                              taatTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGtt                                  >  2:3294480/1‑148 (MQ=255)
                                                                                                              taatTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGtt                                  >  2:1651992/1‑148 (MQ=255)
                                                                                                                atTTTGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTtgg                                <  1:787731/148‑1 (MQ=255)
                                                                                                                  tttGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACGTTTATTTACATTGAGTTAATATTTGATTTTTTTTGATTACAAAACAGACGAAAACTGAATCCAGAGTCGACTGCAGTTACGTtggtg                              >  1:1203185/1‑148 (MQ=255)
                                                                                                                  tttGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGAAGATACGTtggtg                              >  2:2094145/1‑148 (MQ=255)
                                                                                                                   ttGATAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTtggtgg                             <  1:312262/148‑1 (MQ=255)
                                                                                                                      aTAAGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGt                          >  2:2796585/1‑148 (MQ=255)
                                                                                                                         aGCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTAt                       >  2:2407027/1‑148 (MQ=255)
                                                                                                                          gCATTATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTAtt                      <  2:1167747/148‑1 (MQ=255)
                                                                                                                              tATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTa                  >  2:1271342/1‑148 (MQ=255)
                                                                                                                              tATCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTa                  >  2:2365832/1‑148 (MQ=255)
                                                                                                                               aTCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAg                 <  1:2670362/148‑1 (MQ=255)
                                                                                                                               aTCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAg                 <  1:1472290/148‑1 (MQ=255)
                                                                                                                                tCACTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGa                >  2:2007238/1‑148 (MQ=255)
                                                                                                                                  aCTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATg              <  2:443538/148‑1 (MQ=255)
                                                                                                                                   cTTGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATgg             <  1:3486111/148‑1 (MQ=255)
                                                                                                                                    ttGGAGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTGGGTGGGGTTATTTGTAGATGGt            >  2:2870406/1‑148 (MQ=255)
                                                                                                                                        aGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCa        >  2:1070545/1‑148 (MQ=255)
                                                                                                                                        aGCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCa        >  1:1895035/1‑148 (MQ=255)
                                                                                                                                         gCGATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCAg       <  1:2520723/148‑1 (MQ=255)
                                                                                                                                           gATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCAGTa     <  1:2365663/148‑1 (MQ=255)
                                                                                                                                           gATTTTTTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCAGTa     <  1:1271342/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTTTGCAGGTGACTTTTTCTTATATGTTGAAATTTTCACTGCCACGAGAAAGCCTCTCTGCGGATGCGATGCCACTGTTAATCCCGTGATAGTGCTCGTGAAAGTGATAATAATTTTGATAAGCATTATCACTTGGAGCGATTTTCTATCACCCATTGCGCGAAAAAGAAATACATTTATTTACATTGAGTTCATATTTGATGTTTTTTGATTACAAAACAGACGAAAACTGCATCCAGAGGCGAATGCAGTTACGTTGGTGGGGTTATTTGTAGATGGTTGCAGTACCG  >  CP009273/836987‑837276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑