Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 888,964 C→T 100% intergenic (+75/‑276) ybjN → / → potF negative regulator of motility; multicopy suppressor of coaA(Ts)/putrescine transporter subunit: periplasmic‑binding component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273888,9640CT100.0% 899.6 / NA 275intergenic (+75/‑276)ybjN/potFnegative regulator of motility; multicopy suppressor of coaA(Ts)/putrescine transporter subunit: periplasmic‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (119/156);  total (119/156)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 278 total aligned reads displayed.

CCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAG  >  CP009273/888820‑889108
                                                                                                                                                |                                                                                                                                                
 cATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTat                                                                                                                                              >  2:655331/1‑147 (MQ=255)
 cATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTaa                                                                                                                                              >  1:932654/1‑148 (MQ=255)
    cAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGa                                                                                                                                           <  2:2946713/148‑1 (MQ=255)
    cAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGa                                                                                                                                           >  2:2542190/1‑148 (MQ=255)
    cAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGa                                                                                                                                           <  1:2789056/148‑1 (MQ=255)
    cAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGa                                                                                                                                           <  1:2580966/148‑1 (MQ=255)
     aaCTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGat                                                                                                                                          <  1:772307/148‑1 (MQ=255)
      aCTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCCAGCAGTCGAGGGTGCAGTGGCTGCGCGCTGAAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGatt                                                                                                                                         <  1:2831883/148‑1 (MQ=255)
       ctgctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGatta                                                                                                                                        <  2:1392581/148‑1 (MQ=255)
        tgctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGattat                                                                                                                                       <  2:2425139/148‑1 (MQ=255)
        tgctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGattat                                                                                                                                       <  1:678012/148‑1 (MQ=255)
         gctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGattatt                                                                                                                                      >  1:1716292/1‑148 (MQ=255)
          ctgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTAttt                                                                                                                                     <  2:443907/148‑1 (MQ=255)
           tgTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTc                                                                                                                                    <  1:2989504/148‑1 (MQ=255)
            gTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCa                                                                                                                                   <  2:3321285/148‑1 (MQ=255)
               aCCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACtt                                                                                                                                <  1:1320298/148‑1 (MQ=255)
                  gACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTtctc                                                                                                                             <  2:2125829/148‑1 (MQ=255)
                  gACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTtctc                                                                                                                             <  2:930728/148‑1 (MQ=255)
                    cTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAGTTATCTGCTG‑TTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtg                                                                                                                          >  2:609428/1‑148 (MQ=255)
                     tgatgatgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtg                                                                                                                          >  1:3079237/1‑148 (MQ=255)
                      gatgatgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtgt                                                                                                                         >  1:597923/1‑148 (MQ=255)
                       atgatgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTtgtg                                                                                                                        >  1:1292897/1‑148 (MQ=255)
                          atgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTTTCg                                                                                                                     >  2:3190966/1‑148 (MQ=255)
                           tgaAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGa                                                                                                                    <  2:3447783/148‑1 (MQ=255)
                             aaGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGAtt                                                                                                                  <  2:541679/148‑1 (MQ=255)
                               gggCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAAGTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTg                                                                                                                >  2:711582/1‑148 (MQ=255)
                                 gCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGc                                                                                                              <  1:1015740/148‑1 (MQ=255)
                                     aacaacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCtttt                                                                                                          >  2:2067449/1‑147 (MQ=255)
                                     aacaacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTa                                                                                                          >  2:300327/1‑148 (MQ=255)
                                       caacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATc                                                                                                        <  1:1849281/148‑1 (MQ=255)
                                        aacGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATca                                                                                                       <  1:729799/148‑1 (MQ=255)
                                         ccGTTACCGAAAACTCTTGCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATcac                                                                                                      <  2:1984163/147‑1 (MQ=255)
                                         acGTTACCTAAAAATATTTCCTCCAATTATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAAGATTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATcac                                                                                                      <  1:3119924/148‑1 (MQ=255)
                                         acGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATcac                                                                                                      <  1:422186/148‑1 (MQ=255)
                                         acGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATaac                                                                                                      >  2:3104218/1‑148 (MQ=255)
                                          cGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATcaca                                                                                                     <  2:153329/148‑1 (MQ=255)
                                          cGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATcaca                                                                                                     <  1:575592/148‑1 (MQ=255)
                                           gTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACAt                                                                                                    <  1:2401160/148‑1 (MQ=255)
                                              aCCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATaga                                                                                                 <  1:706159/148‑1 (MQ=255)
                                                  aaaaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCaa                                                                                             <  1:470923/148‑1 (MQ=255)
                                                   aaaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCaaa                                                                                            >  1:1656223/1‑148 (MQ=255)
                                                   aaaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCaaa                                                                                            >  2:344019/1‑148 (MQ=255)
                                                    aaCTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTGTTAACCTTTTTTTAATGATTATTTCACTTCTCTTGTGTCGATTTGGCTGTATCACATAGAGCAAat                                                                                           >  2:2676295/1‑148 (MQ=255)
                                                      cTATTTCCTCCACTGAAAACTCCTTTCGAGCAAGCAGTAGCTGGTGCAGTGGCTGCGCGCTGCAATATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTGACTTCTCTTGTGTCGATTTGGCTTGATAACAGAGAGGAAatat                                                                                         >  2:2517351/1‑148 (MQ=255)
                                                       tATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATg                                                                                        <  2:2735190/148‑1 (MQ=255)
                                                       tATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATg                                                                                        >  1:3176159/1‑148 (MQ=255)
                                                       gaTTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATg                                                                                        <  2:1933205/147‑1 (MQ=255)
                                                        aTTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTTGCTTTATCACATAGAGCAAATATGc                                                                                       >  1:785053/1‑148 (MQ=255)
                                                        aTTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGc                                                                                       <  2:2356770/148‑1 (MQ=255)
                                                        aTTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGc                                                                                       <  1:2879184/148‑1 (MQ=255)
                                                          ttCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTAGTGTGTCGATTTGGGTTTATCACATAGAGCAAATATGCAt                                                                                     >  1:3311340/1‑148 (MQ=255)
                                                              tccCCTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATaaaa                                                                                 <  2:2877461/148‑1 (MQ=255)
                                                              tccACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATaaaa                                                                                 <  2:411975/148‑1 (MQ=255)
                                                                cACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAAt                                                                               <  2:3501237/148‑1 (MQ=255)
                                                                 aCTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAAtt                                                                              <  2:1694403/148‑1 (MQ=255)
                                                                  cTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAAttt                                                                             <  1:1916463/148‑1 (MQ=255)
                                                                   tGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTg                                                                            <  1:732425/148‑1 (MQ=255)
                                                                       aaCTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTaa                                                                        <  2:1621147/148‑1 (MQ=255)
                                                                        aCTCCTTTCGAGCACTCAGTCGATGGTGCAGTGGCTGCTCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAATATTATTTCAATTCTCTTGTTTGGATTTGTCTTTATCACATAGATCAAATATGCATAAAAATTTGTTaaa                                                                       <  2:3233593/148‑1 (MQ=255)
                                                                        aCTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTaaa                                                                       <  1:3026106/148‑1 (MQ=255)
                                                                          tCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATa                                                                     >  1:2930639/1‑148 (MQ=255)
                                                                           ccTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATAc                                                                    >  1:221934/1‑148 (MQ=255)
                                                                             tttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCg                                                                  <  1:1205015/148‑1 (MQ=255)
                                                                             tttCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCg                                                                  >  1:2038204/1‑148 (MQ=255)
                                                                               tCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGtt                                                                <  2:3506611/148‑1 (MQ=255)
                                                                                cGAGCACGCAGTCGCTGTTTCAGTGGCTTCGCGCTGCAAAATTATCTGATTTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATATATCAAATATGCATAAAAATTTGTTAAATACCGttt                                                               <  2:23832/148‑1 (MQ=255)
                                                                                cGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGttt                                                               <  2:1997965/148‑1 (MQ=255)
                                                                                 gAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATGTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGtttt                                                              <  2:2857031/148‑1 (MQ=255)
                                                                                    cACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTa                                                           <  1:998154/148‑1 (MQ=255)
                                                                                    cACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTa                                                           <  1:3034099/148‑1 (MQ=255)
                                                                                     aCGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTaa                                                          <  1:249597/148‑1 (MQ=255)
                                                                                          gTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACGGTTTTTTAATCCGa                                                     >  2:3122653/1‑148 (MQ=255)
                                                                                           tCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAg                                                    <  1:1314146/148‑1 (MQ=255)
                                                                                              cTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCta                                                 <  2:1781045/148‑1 (MQ=255)
                                                                                              cTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCta                                                 >  1:3427260/1‑148 (MQ=255)
                                                                                              cTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCACTTGTGTCGATTTGGCTTTATCACATAGAGAAAATAAGCATAAAAATTTGTTAAATACAGTTTTTTAATCAGAGCta                                                 >  2:938766/1‑148 (MQ=255)
                                                                                               tGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCtat                                                <  2:148090/148‑1 (MQ=255)
                                                                                               tGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCtat                                                >  1:211406/1‑148 (MQ=255)
                                                                                                 gTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAg                                              <  1:344019/148‑1 (MQ=255)
                                                                                                     aGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGAATATTTCACTTCTCTTGTGTAGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACAGATTTTTAATCCGAGCTATAGtctc                                          >  1:2049675/1‑148 (MQ=255)
                                                                                                      gTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCa                                         <  1:1723247/148‑1 (MQ=255)
                                                                                                       tGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCaa                                        <  1:2239837/148‑1 (MQ=255)
                                                                                                         gCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAc                                      <  2:612705/148‑1 (MQ=255)
                                                                                                          cTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAAcc                                     >  2:1280872/1‑148 (MQ=255)
                                                                                                            gcgcgcTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCt                                   >  1:1033706/1‑148 (MQ=255)
                                                                                                                 cTGCAAAATTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTa                              >  1:3264386/1‑148 (MQ=255)
                                                                                                                       aaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTa                        >  2:2795968/1‑148 (MQ=255)
                                                                                                                       aaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTa                        >  2:1043013/1‑148 (MQ=255)
                                                                                                                       aaTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTa                        <  1:2372999/148‑1 (MQ=255)
                                                                                                                        aTTATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAt                       <  2:1115901/148‑1 (MQ=255)
                                                                                                                         ttATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTAtt                      >  1:3021784/1‑148 (MQ=255)
                                                                                                                          tATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTc                     >  2:2614865/1‑148 (MQ=255)
                                                                                                                          tATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTc                     >  1:362579/1‑148 (MQ=255)
                                                                                                                          tATCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTc                     >  2:872918/1‑148 (MQ=255)
                                                                                                                           aTCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCCAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCt                    >  1:1393531/1‑148 (MQ=255)
                                                                                                                           aTCTGCTGTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCt                    <  1:733558/148‑1 (MQ=255)
                                                                                                                             ctgctgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTg                  <  1:951667/148‑1 (MQ=255)
                                                                                                                               gctgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCg                >  1:1109493/1‑148 (MQ=255)
                                                                                                                                ctgTTTTTAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGa               >  2:3309372/1‑148 (MQ=255)
                                                                                                                                      ttAACCTTTTTTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCttt         <  2:2216935/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CCATCAACTGCTGTTACCGACTGATGATGAAGGGCAAAACAACGTTACCGAAAACTATTTCCTCCACTGATAACTCCTTTCGAGCACGCAGTCGCTGGTGCAGTGGCTGCGCGCTGCAAAATTATCTGCTGTTTTTAACCTTTTCTTAAAGATTATTTCACTTCTCTTGTGTCGATTTGGCTTTATCACATAGAGCAAATATGCATAAAAATTTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATGCTTTTATATAG  >  CP009273/888820‑889108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑