Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 955,568 A→T 100% intergenic (+17/‑152) aroA → / → ycaL 5‑enolpyruvylshikimate‑3‑phosphate synthetase/putative peptidase‑related chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273955,5680AT99.0% 621.7 / ‑2.6 191intergenic (+17/‑152)aroA/ycaL5‑enolpyruvylshikimate‑3‑phosphate synthetase/putative peptidase‑related chaperone
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (67/122);  total (69/122)
Fisher's exact test for biased strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 196 total aligned reads displayed.

CGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGA  >  CP009273/955423‑955711
                                                                                                                                                 |                                                                                                                                               
cGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCaattaa                                                                                                                                               >  1:3050295/1‑148 (MQ=255)
cGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCaattaa                                                                                                                                               <  2:2817868/148‑1 (MQ=255)
 ggATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGTTTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCaattaat                                                                                                                                              <  2:906340/148‑1 (MQ=255)
 ggATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCaattaat                                                                                                                                              <  2:1646958/148‑1 (MQ=255)
    tGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGc                                                                                                                                           <  1:1706735/148‑1 (MQ=255)
        gATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCaaa                                                                                                                                       <  2:850725/148‑1 (MQ=255)
         aTGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAAt                                                                                                                                      <  2:722727/148‑1 (MQ=255)
         aTGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAAt                                                                                                                                      >  2:2461777/1‑148 (MQ=255)
         aTGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCAGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAAt                                                                                                                                      >  2:505/1‑148 (MQ=255)
          tgtgTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAAtc                                                                                                                                     <  2:1450457/148‑1 (MQ=255)
             gtTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAAtcttt                                                                                                                                  <  2:2694154/148‑1 (MQ=255)
             gtTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAAtcttt                                                                                                                                  <  1:41174/148‑1 (MQ=255)
              tttCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAAtctttc                                                                                                                                 <  2:180834/148‑1 (MQ=255)
               ttCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGNTTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAAtctttct                                                                                                                                >  1:1783564/1‑148 (MQ=255)
               ttCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAAtctttct                                                                                                                                <  2:177627/148‑1 (MQ=255)
                tctcGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAAtctttctt                                                                                                                               <  1:1771074/148‑1 (MQ=255)
                  tcGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTa                                                                                                                             <  2:1972147/148‑1 (MQ=255)
                  tcGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTa                                                                                                                             <  2:362292/148‑1 (MQ=255)
                              ttGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCgg                                                                                                                 <  1:3107594/148‑1 (MQ=255)
                              ttGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCgg                                                                                                                 >  2:212542/1‑148 (MQ=255)
                               tGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGc                                                                                                                <  2:2621508/148‑1 (MQ=255)
                                gTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGca                                                                                                               <  1:580904/148‑1 (MQ=255)
                                  cAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGcaca                                                                                                             <  1:1981759/148‑1 (MQ=255)
                                    gATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACAtt                                                                                                           >  2:3360857/1‑148 (MQ=255)
                                     aTACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTg                                                                                                          >  1:3140266/1‑148 (MQ=255)
                                      tACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGt                                                                                                         >  1:2333071/1‑148 (MQ=255)
                                      tACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGt                                                                                                         <  2:3188582/148‑1 (MQ=255)
                                        cacCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCg                                                                                                       >  2:886417/1‑148 (MQ=255)
                                          ccAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGc                                                                                                     >  1:488790/1‑148 (MQ=255)
                                           cAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCg                                                                                                    <  1:1307064/148‑1 (MQ=255)
                                            aGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGt                                                                                                   <  1:3221344/148‑1 (MQ=255)
                                             gTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGtt                                                                                                  >  2:3411514/1‑148 (MQ=255)
                                              tGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGttt                                                                                                 >  1:2577881/1‑148 (MQ=255)
                                              tGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGttt                                                                                                 >  1:3068937/1‑148 (MQ=255)
                                               gACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGtttt                                                                                                <  2:1608914/148‑1 (MQ=255)
                                               gACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGtttt                                                                                                <  2:1608904/148‑1 (MQ=255)
                                                aCGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGttttt                                                                                               <  1:1264665/148‑1 (MQ=255)
                                                aCGATTATTGATCCCAAATGCACCCCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGttttt                                                                                               <  1:2733582/148‑1 (MQ=255)
                                                 cGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGtgtttt                                                                                              >  1:1967588/1‑148 (MQ=255)
                                                   aTTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGtttttttt                                                                                            <  1:2905971/148‑1 (MQ=255)
                                                    ttCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTc                                                                                           >  2:2009212/1‑148 (MQ=255)
                                                       tttaTCACAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGa                                                                                        <  2:2989169/145‑1 (MQ=255)
                                                         gATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGAcc                                                                                      <  1:114478/148‑1 (MQ=255)
                                                         gATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGAcc                                                                                      >  1:43391/1‑148 (MQ=255)
                                                          aTCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCt                                                                                     <  2:789140/148‑1 (MQ=255)
                                                          aTCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCt                                                                                     <  1:1017914/148‑1 (MQ=255)
                                                          aTCCAAAATGCACGGCAAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCt                                                                                     <  1:798241/148‑1 (MQ=255)
                                                            cccAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCCGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACAGTGTCGGCGTTTTTTGTCGGACCTtg                                                                                   >  2:1677205/1‑148 (MQ=255)
                                                            cccAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTtg                                                                                   <  2:3266853/148‑1 (MQ=255)
                                                            cccAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTtg                                                                                   <  2:643386/148‑1 (MQ=255)
                                                             ccAAATGCACGGCCAAACCAGTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCgtgt                                                                                  <  2:2376123/148‑1 (MQ=255)
                                                               aaaTGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGa                                                                                <  1:1532651/148‑1 (MQ=255)
                                                               aaaTGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGACCATGTGa                                                                                >  2:2961475/1‑148 (MQ=255)
                                                                 aTGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGt                                                                              <  2:863839/148‑1 (MQ=255)
                                                                 aTGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGt                                                                              >  1:3467567/1‑148 (MQ=255)
                                                                   gCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCa                                                                            <  1:818275/148‑1 (MQ=255)
                                                                      cGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCAttt                                                                         <  1:666206/148‑1 (MQ=255)
                                                                      cGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGACCTTGTGAGTCAttt                                                                         >  2:1870603/1‑148 (MQ=255)
                                                                          cAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGACCTTGTGAGTGATTTTGAt                                                                     >  2:3147725/1‑148 (MQ=255)
                                                                            aaaCCTTTCCGTATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTa                                                                   <  2:1142466/148‑1 (MQ=255)
                                                                             aaCATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTTGGACCTTTTGGGTCAtgttttttta                                                                  >  1:389091/1‑142 (MQ=255)
                                                                             aaCATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTaa                                                                  >  2:2532423/1‑148 (MQ=255)
                                                                             aaCATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGACCTTGTGATTCATTTTGTTTaa                                                                  >  1:1716875/1‑148 (MQ=255)
                                                                                  ttCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTa                                                             >  2:2454263/1‑148 (MQ=255)
                                                                                     cGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCg                                                          <  1:1949018/148‑1 (MQ=255)
                                                                                        attattTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGTCCTTGTGTGTCATTTTGGTTAATGGTCGCGTCg                                                       >  2:625654/1‑148 (MQ=255)
                                                                                         ttattTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGc                                                      >  2:1384061/1‑148 (MQ=255)
                                                                                            tttCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTGGGACCTTGTGTGTCATTTTGATTAATGGTAGCGTCGCGTg                                                   >  2:2670476/1‑148 (MQ=255)
                                                                                            tttCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTg                                                   <  1:3435436/148‑1 (MQ=255)
                                                                                                   cagcTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTGTGATTACTGGTAGCGTCGCTTGTCAATGt                                            >  2:3446872/1‑148 (MQ=255)
                                                                                                     gcTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTaa                                          <  2:1284884/148‑1 (MQ=255)
                                                                                                       tGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgt                                        <  2:1744282/148‑1 (MQ=255)
                                                                                                         gcgcGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgttg                                      <  2:1618667/148‑1 (MQ=255)
                                                                                                          cgcgGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgttgt                                     <  1:1641244/148‑1 (MQ=255)
                                                                                                              gATTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGAt                                 <  2:2403492/148‑1 (MQ=255)
                                                                                                               aTTAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATa                                <  2:1932155/148‑1 (MQ=255)
                                                                                                                ttAGCCAGGCAGCCTGAATGAACACCGGGCAATTAAGAGCCAAATCTTTCTTTATCACACCGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATAc                               <  1:2328726/148‑1 (MQ=255)
                                                                                                                ttAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATAc                               <  2:782010/148‑1 (MQ=255)
                                                                                                                 tAGCCAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTGGGACCTTGTGTGTCATTTTGATTAATTGTATCGTCGCGGTTGAATGTTAGtttttgtttaa                              >  2:1494275/1‑143 (MQ=255)
                                                                                                                    ccAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACataa                           <  2:2493569/148‑1 (MQ=255)
                                                                                                                    ccAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACataa                           <  2:2877327/148‑1 (MQ=255)
                                                                                                                     cAGGCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAACACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACataat                          <  1:2130844/148‑1 (MQ=255)
                                                                                                                       ggCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAatat                        <  1:2665526/148‑1 (MQ=255)
                                                                                                                       ggCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAatat                        <  1:910381/148‑1 (MQ=255)
                                                                                                                       ggCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAatat                        <  2:2940460/148‑1 (MQ=255)
                                                                                                                       ggCAGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAatat                        <  2:2940459/148‑1 (MQ=255)
                                                                                                                          aGCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTta                     <  2:42223/148‑1 (MQ=255)
                                                                                                                           gCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTtat                    <  2:3125844/148‑1 (MQ=255)
                                                                                                                           gCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTtat                    <  2:608707/148‑1 (MQ=255)
                                                                                                                           gCCTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCGTGTCAAAGTTAGTTGTTGATACAAAATATTtat                    >  2:2726476/1‑148 (MQ=255)
                                                                                                                            ccTGAATGAACAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTtata                   <  1:2439111/148‑1 (MQ=255)
                                                                                                                                  tgaaCAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCTCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGAt             <  1:3491509/148‑1 (MQ=255)
                                                                                                                                  tgaaCAACGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTGAATGTAAGTTGTTGATAAATAAAATTTATATATGAt             >  2:1184440/1‑148 (MQ=255)
                                                                                                                                    aacaacGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTa           <  1:3262528/148‑1 (MQ=255)
                                                                                                                                     acaacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGGCCTTGTGTGTCATTTTGATTAATGGGAGCGTCGCGTGTGCATGTAAGCTGTTGTTTAATAATATTTATATATGATTaa          >  2:346254/1‑148 (MQ=255)
                                                                                                                                      caacGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAAt         <  1:2461777/148‑1 (MQ=255)
                                                                                                                                      caacGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGAACTTTTTTGTAATATTGAATAATGGTAGCCTCGCTTGTCAAACTTAGTTTTTTATACAAAATATTTTTATATAATTaaa         >  2:1279892/1‑147 (MQ=255)
                                                                                                                                         cGGGCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTGGGACCTTGTGTGTCATTTTGATTAATTGTAGCGTCGCGTGGGAAAGTAATTTTTTTTTAAAAAAAAATTATATTTTATTTATaaa      >  2:1240932/1‑148 (MQ=255)
                                                                                                                                            gCAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACgg   <  1:856616/148‑1 (MQ=255)
                                                                                                                                             cAATTAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGga  <  2:1005530/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CGGATGGCGATGTGTTTCTCGCTGGTGGCGTTGTCAGATACACCAGTGACGATTCTTGATCCCAAATGCACGGCCAAAACATTTCCGGATTATTTCGAGCAGCTGGCGCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGA  >  CP009273/955423‑955711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑