Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 994,328 G→T 100% G128V (GGA→GTA)  elfD → putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273994,3280GT99.5% 668.2 / ‑4.1 202G128V (GGA→GTA) elfDputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (104/97);  total (104/98)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 202 total aligned reads displayed.

TTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTT  >  CP009273/994184‑994471
                                                                                                                                                |                                                                                                                                               
ttaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTaaa                                                                                                                                              <  1:1973359/148‑1 (MQ=255)
  acacCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATa                                                                                                                                            >  2:533912/1‑148 (MQ=255)
    accaccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATAcc                                                                                                                                          >  1:1856598/1‑148 (MQ=255)
          gCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAg                                                                                                                                    <  1:3233150/148‑1 (MQ=255)
            tATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGAt                                                                                                                                  <  2:2492231/148‑1 (MQ=255)
            tATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGAt                                                                                                                                  >  2:3376416/1‑148 (MQ=255)
               ttGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGc                                                                                                                               >  1:463978/1‑148 (MQ=255)
                   gCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCcaca                                                                                                                           <  1:485586/148‑1 (MQ=255)
                      gAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACaa                                                                                                                        <  1:1131768/148‑1 (MQ=255)
                      gAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACaa                                                                                                                        <  2:2000099/148‑1 (MQ=255)
                          ccGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCg                                                                                                                    >  1:1526278/1‑148 (MQ=255)
                            ggCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGtc                                                                                                                  <  1:1283428/148‑1 (MQ=255)
                              cAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGtcat                                                                                                                >  1:3319124/1‑148 (MQ=255)
                              cAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGtcat                                                                                                                >  2:2606611/1‑148 (MQ=255)
                                aaTGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGtcatca                                                                                                              >  2:1808366/1‑148 (MQ=255)
                                  tGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCaaa                                                                                                            >  1:2886960/1‑148 (MQ=255)
                                    agaATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACt                                                                                                          <  1:3262056/148‑1 (MQ=255)
                                      aaTCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACttt                                                                                                        >  1:3145938/1‑148 (MQ=255)
                                      aaTCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACttt                                                                                                        >  1:307793/1‑148 (MQ=255)
                                      aaTCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACttt                                                                                                        >  1:3514907/1‑148 (MQ=255)
                                      aaTCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACttt                                                                                                        <  2:3459592/148‑1 (MQ=255)
                                       aTCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACtttt                                                                                                       <  1:1730736/148‑1 (MQ=255)
                                        tCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTc                                                                                                      <  2:3247963/148‑1 (MQ=255)
                                          tGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTg                                                                                                    >  2:194296/1‑148 (MQ=255)
                                              aCGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGa                                                                                                <  1:2806668/148‑1 (MQ=255)
                                                  aTTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCaa                                                                                            >  2:636274/1‑148 (MQ=255)
                                                   ttATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCaaa                                                                                           <  2:1733975/148‑1 (MQ=255)
                                                    tATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCaaaa                                                                                          >  1:3056744/1‑148 (MQ=255)
                                                       gTACATTGGAGCGCCGTTGGCGAAAGACCGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGGCATCAAACTTTTCTGGCGACCAAAAGGt                                                                                       >  2:2531171/1‑148 (MQ=255)
                                                       gTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGt                                                                                       >  2:2948008/1‑148 (MQ=255)
                                                        tACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGtt                                                                                      <  1:974295/148‑1 (MQ=255)
                                                         aCATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGttt                                                                                     <  1:299175/148‑1 (MQ=255)
                                                         aCATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGttt                                                                                     <  2:2886960/148‑1 (MQ=255)
                                                                   gcCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACTAAGCCTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATccc                                                                           <  1:3448572/148‑1 (MQ=255)
                                                                    ccGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCt                                                                          <  2:1779013/148‑1 (MQ=255)
                                                                     cGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCtt                                                                         <  2:873682/148‑1 (MQ=255)
                                                                      gTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTa                                                                        >  2:1891225/1‑148 (MQ=255)
                                                                       ttGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAAAGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAg                                                                       >  1:2677215/1‑148 (MQ=255)
                                                                        tGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAgg                                                                      <  2:1720721/148‑1 (MQ=255)
                                                                          gCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCg                                                                    >  2:1634852/1‑148 (MQ=255)
                                                                             aaaGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAgg                                                                 >  1:2930041/1‑148 (MQ=255)
                                                                               aGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCt                                                               <  1:533912/148‑1 (MQ=255)
                                                                               aGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCt                                                               >  2:555096/1‑148 (MQ=255)
                                                                               aGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCt                                                               <  2:514565/148‑1 (MQ=255)
                                                                               aGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCt                                                               <  2:514505/148‑1 (MQ=255)
                                                                                gACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTc                                                              >  1:3477341/1‑148 (MQ=255)
                                                                                 aCAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTcc                                                             >  2:1399134/1‑148 (MQ=255)
                                                                                    gagaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGc                                                          >  1:2455493/1‑148 (MQ=255)
                                                                                    gagaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGc                                                          <  2:7570/148‑1 (MQ=255)
                                                                                    gagaAACCCTTTTCTTCACGAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGc                                                          <  2:7757/148‑1 (MQ=255)
                                                                                     agaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCg                                                         >  2:455799/1‑148 (MQ=255)
                                                                                     agaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCg                                                         >  1:2772588/1‑148 (MQ=255)
                                                                                     agaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCg                                                         >  2:2220923/1‑148 (MQ=255)
                                                                                       aaaCCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGaa                                                       <  1:846459/148‑1 (MQ=255)
                                                                                       aaaCCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGaa                                                       >  2:2049949/1‑148 (MQ=255)
                                                                                         aCCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAAc                                                     <  2:2064256/148‑1 (MQ=255)
                                                                                          cccTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACt                                                    >  2:353154/1‑148 (MQ=255)
                                                                                               ttcttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCTGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGtt                                               <  1:1877063/148‑1 (MQ=255)
                                                                                                  ttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCa                                            <  1:2332801/148‑1 (MQ=255)
                                                                                                  ttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCa                                            <  1:1399134/148‑1 (MQ=255)
                                                                                                     aCTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACtt                                         <  2:3429984/148‑1 (MQ=255)
                                                                                                      cTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTc                                        <  2:3471833/148‑1 (MQ=255)
                                                                                                      cTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTc                                        <  2:3154359/148‑1 (MQ=255)
                                                                                                      cTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTc                                        >  2:1768060/1‑148 (MQ=255)
                                                                                                       tAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCg                                       >  1:566525/1‑148 (MQ=255)
                                                                                                         gCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTc                                     <  2:2796908/148‑1 (MQ=255)
                                                                                                          cGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCa                                    >  1:641816/1‑148 (MQ=255)
                                                                                                          cGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCa                                    >  1:3509901/1‑148 (MQ=255)
                                                                                                          cGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCa                                    >  2:114933/1‑148 (MQ=255)
                                                                                                           gTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAg                                   <  1:607422/148‑1 (MQ=255)
                                                                                                             aCGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCt                                 >  1:869547/1‑148 (MQ=255)
                                                                                                             aCGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCt                                 >  1:2394204/1‑148 (MQ=255)
                                                                                                             aCGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCt                                 >  1:869325/1‑148 (MQ=255)
                                                                                                             aCGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCt                                 >  2:410995/1‑148 (MQ=255)
                                                                                                                ggCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGAc                              <  2:1556785/148‑1 (MQ=255)
                                                                                                                ggCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGAc                              >  1:2290345/1‑148 (MQ=255)
                                                                                                                   aGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATg                           <  1:1612646/148‑1 (MQ=255)
                                                                                                                    gTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATgg                          <  2:3414205/148‑1 (MQ=255)
                                                                                                                      cccTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGtt                        <  1:2090239/148‑1 (MQ=255)
                                                                                                                       ccTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTa                       >  2:552552/1‑148 (MQ=255)
                                                                                                                        cTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTAc                      >  1:3409247/1‑148 (MQ=255)
                                                                                                                           caacaaCGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGt                   <  2:312225/148‑1 (MQ=255)
                                                                                                                           caacaaCGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGt                   <  2:656399/148‑1 (MQ=255)
                                                                                                                            aacaacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTTCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTc                  <  2:3215138/148‑1 (MQ=255)
                                                                                                                            aacaacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTc                  >  1:10866/1‑148 (MQ=255)
                                                                                                                             acaacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCa                 >  2:3063286/1‑148 (MQ=255)
                                                                                                                              caacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAg                >  2:1025218/1‑148 (MQ=255)
                                                                                                                              caacGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAg                <  2:1606761/148‑1 (MQ=255)
                                                                                                                                acGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTa              <  1:2606611/148‑1 (MQ=255)
                                                                                                                                 cGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTaa             >  1:1712219/1‑148 (MQ=255)
                                                                                                                                  gAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAAc            <  2:3433225/148‑1 (MQ=255)
                                                                                                                                    aGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAAccc          >  2:1462190/1‑148 (MQ=255)
                                                                                                                                     gCGGATAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCa         <  1:1830231/148‑1 (MQ=255)
                                                                                                                                      cGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCaa        <  2:1449894/148‑1 (MQ=255)
                                                                                                                                         aaaGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAacac     >  2:2281472/1‑148 (MQ=255)
                                                                                                                                         aaaGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAacac     >  2:1269856/1‑148 (MQ=255)
                                                                                                                                          aaGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACAcc    <  2:191237/148‑1 (MQ=255)
                                                                                                                                           agagGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCt   <  1:994720/148‑1 (MQ=255)
                                                                                                                                            gagGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCtt  <  1:1651186/148‑1 (MQ=255)
                                                                                                                                            gagGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCtt  >  1:2467264/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTT  >  CP009273/994184‑994471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑