Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,146,692 A→T 100% T189T (ACA→ACT)  fabG → 3‑oxoacyl‑[acyl‑carrier‑protein] reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,146,6920AT96.8% 802.1 / 11.5 247T189T (ACA→ACT) fabG3‑oxoacyl‑[acyl‑carrier‑protein] reductase
Reads supporting (aligned to +/- strand):  ref base A (3/5);  new base T (126/113);  total (129/118)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 249 total aligned reads displayed.

GTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAAC  >  CP009273/1146547‑1146839
                                                                                                                                                 |                                                                                                                                                   
gtACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCg                                                                                                                                                   <  1:151777/148‑1 (MQ=255)
    cATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTgcg                                                                                                                                               <  1:1103246/148‑1 (MQ=255)
    cATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTgcg                                                                                                                                               <  1:1758602/148‑1 (MQ=255)
    cATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGAGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTgcg                                                                                                                                               <  1:2823507/148‑1 (MQ=255)
     aTGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTgcgc                                                                                                                                              >  1:2023381/1‑148 (MQ=255)
       gggAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTg                                                                                                                                            <  2:1781094/148‑1 (MQ=255)
       gggAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTg                                                                                                                                            >  2:2719003/1‑148 (MQ=255)
        ggAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGa                                                                                                                                           >  1:3253676/1‑148 (MQ=255)
           aaTGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCg                                                                                                                                        <  1:1058238/148‑1 (MQ=255)
            aTGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGa                                                                                                                                       >  1:593823/1‑148 (MQ=255)
            aTGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGa                                                                                                                                       >  2:2932144/1‑148 (MQ=255)
             tGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGAt                                                                                                                                      >  2:2177402/1‑148 (MQ=255)
             tGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGAt                                                                                                                                      <  1:2123743/148‑1 (MQ=255)
              ggCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATg                                                                                                                                     <  1:850764/148‑1 (MQ=255)
               gCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGa                                                                                                                                    >  2:3200843/1‑148 (MQ=255)
                  gTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCa                                                                                                                                 >  2:1164705/1‑148 (MQ=255)
                   tCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAg                                                                                                                                >  2:3241788/1‑148 (MQ=255)
                      ggCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGt                                                                                                                             >  1:2021718/1‑148 (MQ=255)
                       gCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTg                                                                                                                            >  1:2698720/1‑148 (MQ=255)
                         cAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCg                                                                                                                          <  2:3012521/148‑1 (MQ=255)
                         cAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCg                                                                                                                          <  2:3058308/148‑1 (MQ=255)
                          aaCTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCgg                                                                                                                         >  2:1483433/1‑148 (MQ=255)
                           aCTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCggg                                                                                                                        <  2:1302857/148‑1 (MQ=255)
                              aCGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTAt                                                                                                                     <  2:2350697/148‑1 (MQ=255)
                               cGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATc                                                                                                                    >  2:1111309/1‑148 (MQ=255)
                               cGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATc                                                                                                                    <  2:3354909/148‑1 (MQ=255)
                                   gcggcgAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTCTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTgg                                                                                                                >  2:452973/1‑148 (MQ=255)
                                       cgAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCa                                                                                                            >  1:824258/1‑148 (MQ=255)
                                        gAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATACTGGCGCAg                                                                                                           <  1:2545497/148‑1 (MQ=255)
                                          aaGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGt                                                                                                         <  1:2247823/148‑1 (MQ=255)
                                            gCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTc                                                                                                       >  2:71283/1‑148 (MQ=255)
                                            gCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGGTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTc                                                                                                       <  1:2776946/148‑1 (MQ=255)
                                             cGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTcc                                                                                                      >  1:1019636/1‑148 (MQ=255)
                                             cGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTcc                                                                                                      >  1:3283846/1‑148 (MQ=255)
                                                gCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGc                                                                                                   >  1:1964010/1‑148 (MQ=255)
                                                  ttGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCgg                                                                                                 >  2:104888/1‑148 (MQ=255)
                                                  ttGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCgg                                                                                                 <  1:2983633/148‑1 (MQ=255)
                                                   tGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCggg                                                                                                >  2:1971367/1‑148 (MQ=255)
                                                        ggCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGcc                                                                                           <  2:2871692/148‑1 (MQ=255)
                                                          cTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTc                                                                                         >  2:1407153/1‑148 (MQ=255)
                                                            tCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTcgg                                                                                       >  1:3188344/1‑148 (MQ=255)
                                                            tCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTcgg                                                                                       >  1:2279545/1‑148 (MQ=255)
                                                             cAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTcggc                                                                                      <  1:1533414/148‑1 (MQ=255)
                                                                  aaTCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGgcgc                                                                                 >  1:3505806/1‑148 (MQ=255)
                                                                   aTCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGca                                                                                <  2:2135484/148‑1 (MQ=255)
                                                                   aTCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGca                                                                                >  2:744044/1‑148 (MQ=255)
                                                                         ggCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGaa                                                                          <  1:738843/148‑1 (MQ=255)
                                                                          gcgcgcGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGaaa                                                                         <  2:3312574/148‑1 (MQ=255)
                                                                           cgcgcgAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAAt                                                                        <  1:2618776/148‑1 (MQ=255)
                                                                                   gTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAAcg                                                                >  1:3333609/1‑148 (MQ=255)
                                                                                    ttGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAACTCGCCAAcgc                                                               >  2:3408559/1‑148 (MQ=255)
                                                                                    ttGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGAGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCTGGTCGCCTCGGCGGCGCACAGGAAATCGCCAAcgc                                                               >  1:290768/1‑148 (MQ=255)
                                                                                     tGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAAcgcg                                                              >  2:2132739/1‑148 (MQ=255)
                                                                                     tGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAAcgcg                                                              >  2:1076202/1‑148 (MQ=255)
                                                                                      gCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCgg                                                             <  2:1360569/148‑1 (MQ=255)
                                                                                      gCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCgg                                                             <  2:3039251/148‑1 (MQ=255)
                                                                                          cACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGc                                                         >  2:877816/1‑148 (MQ=255)
                                                                                          cACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGc                                                         <  1:1225904/148‑1 (MQ=255)
                                                                                           aCGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCa                                                        >  2:390288/1‑148 (MQ=255)
                                                                                           aCGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCa                                                        <  2:1855114/148‑1 (MQ=255)
                                                                                            cgcgGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCAt                                                       >  2:12656/1‑148 (MQ=255)
                                                                                            cgcgGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCAt                                                       >  2:12674/1‑148 (MQ=255)
                                                                                            cgcgGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCAt                                                       <  1:1860880/148‑1 (MQ=255)
                                                                                             gcgGTATTACTGTAAACGTTGGTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCAtt                                                      <  1:572475/148‑1 (MQ=255)
                                                                                              cgGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTc                                                     <  1:3158811/148‑1 (MQ=255)
                                                                                               ggTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTcc                                                    <  1:295335/148‑1 (MQ=255)
                                                                                                  aTTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTgg                                                 <  1:1266571/148‑1 (MQ=255)
                                                                                                   ttACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGc                                                >  1:1022430/1‑148 (MQ=255)
                                                                                                   ttACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGc                                                >  2:262809/1‑148 (MQ=255)
                                                                                                     aCTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCAt                                              <  1:1307183/148‑1 (MQ=255)
                                                                                                     aCTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCAt                                              <  1:1307975/148‑1 (MQ=255)
                                                                                                      cTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATc                                             >  2:1992958/1‑148 (MQ=255)
                                                                                                       tGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATcc                                            >  2:2299059/1‑148 (MQ=255)
                                                                                                         tAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcga                                          <  1:1164717/148‑1 (MQ=255)
                                                                                                         tAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcga                                          <  2:2431609/148‑1 (MQ=255)
                                                                                                          aaaCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgac                                         >  2:1624547/1‑148 (MQ=255)
                                                                                                            aCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                       <  2:1208464/148‑1 (MQ=255)
                                                                                                            aCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                       >  2:739782/1‑148 (MQ=255)
                                                                                                            aCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                       >  1:1177456/1‑148 (MQ=255)
                                                                                                            aCGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCcgacga                                       <  2:1951517/148‑1 (MQ=255)
                                                                                                               ttgttgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAagc                                    >  2:604744/1‑148 (MQ=255)
                                                                                                               ttgttgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAagc                                    >  2:3479180/1‑148 (MQ=255)
                                                                                                                tgttgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAagca                                   >  2:16593/1‑148 (MQ=255)
                                                                                                                 gttgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAagcag                                  <  1:1766603/148‑1 (MQ=255)
                                                                                                                  ttgCTCCGTGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGAGCACAGGAATTCGCCAACGCGGTTGCATTCCTGGCATCCGACGAagcagc                                 <  1:1412111/148‑1 (MQ=255)
                                                                                                                   tgCTCCGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCt                                >  1:635046/1‑148 (MQ=255)
                                                                                                                       ccGGGCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACa                            >  1:2451124/1‑148 (MQ=255)
                                                                                                                         gggCTTTATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATc                          <  1:1483433/148‑1 (MQ=255)
                                                                                                                              ttATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACggg                     <  1:3053729/148‑1 (MQ=255)
                                                                                                                              ttATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACggg                     <  1:2898217/148‑1 (MQ=255)
                                                                                                                               tATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGt                    <  2:1029132/148‑1 (MQ=255)
                                                                                                                               tATTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGCGt                    <  2:2977929/148‑1 (MQ=255)
                                                                                                                                aTTGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTg                   >  2:2067119/1‑148 (MQ=255)
                                                                                                                                  tGAAACGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGaa                 <  1:1111310/148‑1 (MQ=255)
                                                                                                                                      aCGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACtt             <  1:739242/148‑1 (MQ=255)
                                                                                                                                       cGGACATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACttt            >  1:766576/1‑148 (MQ=255)
                                                                                                                                          aCATGACTCGTTCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCa         >  1:3363576/1‑148 (MQ=255)
                                                                                                                                          aCATGACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCa         <  1:2134305/148‑1 (MQ=255)
                                                                                                                                              gACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCAtgtg     >  2:3221869/1‑148 (MQ=255)
                                                                                                                                              gACTCGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCAtgtg     >  1:549385/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GTACCATGGGAAATGGCGGTCAGGCCAACTACGCTGCGGCGAAAGCGGGCTTGATCGGCTTCAGTAAATCACTGGCGCGCGAAGTTGCGTCACGCGGTATTACTGTAAACGTTGTTGCTCCGGGCTTTATTGAAACGGACATGACACGTGCGCTGAGCGATGACCAGCGTGCGGGTATCCTGGCGCAGGTTCCTGCGGGTCGCCTCGGCGGCGCACAGGAAATCGCCAACGCGGTTGCATTCCTGGCATCCGACGAAGCAGCTTACATCACGGGTGAAACTTTGCATGTGAAC  >  CP009273/1146547‑1146839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑