Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,264,442 G→T 100% V274V (GTG→GTT)  kdsA → 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,264,4420GT97.8% 555.9 / 3.5 179V274V (GTG→GTT) kdsA3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (91/84);  total (93/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 179 total aligned reads displayed.

AGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGG  >  CP009273/1264298‑1264582
                                                                                                                                                |                                                                                                                                            
aGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTaaa                                                                                                                                           <  2:3485028/148‑1 (MQ=255)
      tATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTc                                                                                                                                     >  2:1742338/1‑148 (MQ=255)
        tGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCga                                                                                                                                   >  1:1298406/1‑148 (MQ=255)
        tGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCga                                                                                                                                   <  1:997432/148‑1 (MQ=255)
        tGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCga                                                                                                                                   >  1:1484844/1‑148 (MQ=255)
         ggCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCgaa                                                                                                                                  >  2:96978/1‑148 (MQ=255)
         ggCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCgaa                                                                                                                                  <  1:2239771/148‑1 (MQ=255)
             gTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAAc                                                                                                                              >  1:291447/1‑148 (MQ=255)
             gTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAAc                                                                                                                              >  2:3317645/1‑148 (MQ=255)
              tAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACt                                                                                                                             >  1:2445995/1‑148 (MQ=255)
              tAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACt                                                                                                                             <  1:478388/148‑1 (MQ=255)
                ggTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTgg                                                                                                                           <  1:3023282/148‑1 (MQ=255)
                ggTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTgg                                                                                                                           >  1:761183/1‑148 (MQ=255)
                 gTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGa                                                                                                                          <  1:2843817/148‑1 (MQ=255)
                  tCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGAt                                                                                                                         <  1:605073/148‑1 (MQ=255)
                         gggCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCa                                                                                                                  >  1:1556070/1‑148 (MQ=255)
                              gTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTaa                                                                                                             <  1:1861953/148‑1 (MQ=255)
                              gTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTaa                                                                                                             <  2:3277649/148‑1 (MQ=255)
                                  aTTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCtt                                                                                                         >  2:1124826/1‑148 (MQ=255)
                                    tGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCtttt                                                                                                       >  2:2836745/1‑148 (MQ=255)
                                    tGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCtttt                                                                                                       <  1:903724/148‑1 (MQ=255)
                                    tGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCtttt                                                                                                       >  2:1280637/1‑148 (MQ=255)
                                     gAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCttttt                                                                                                      <  1:2546135/148‑1 (MQ=255)
                                      aaGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCtttttt                                                                                                     >  1:702186/1‑148 (MQ=255)
                                      aaGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCtttttt                                                                                                     >  1:2472836/1‑148 (MQ=255)
                                       aGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTg                                                                                                    <  1:2773150/148‑1 (MQ=255)
                                         cgcATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCt                                                                                                  <  1:3113861/148‑1 (MQ=255)
                                             tccggatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGaa                                                                                              >  1:245837/1‑148 (MQ=255)
                                               ccgatccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGaaaa                                                                                            <  1:2396495/146‑1 (MQ=255)
                                                   tccggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATaa                                                                                        <  2:1433380/148‑1 (MQ=255)
                                                     cggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAg                                                                                      <  1:3357948/148‑1 (MQ=255)
                                                     cggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAg                                                                                      <  1:2595911/148‑1 (MQ=255)
                                                      ggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGt                                                                                     <  1:2698064/148‑1 (MQ=255)
                                                      ggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGt                                                                                     <  2:60467/148‑1 (MQ=255)
                                                      ggaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGt                                                                                     <  2:3067732/148‑1 (MQ=255)
                                                       gaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTa                                                                                    <  1:1444112/148‑1 (MQ=255)
                                                       gaACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTa                                                                                    <  2:2915504/148‑1 (MQ=255)
                                                        aaCATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGaaaa                                                                                            >  2:477911/1‑139 (MQ=255)
                                                        aaCATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGaaaa                                                                                            <  1:477911/139‑1 (MQ=255)
                                                        aaCATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATATGGTTAAAGGTTTCGAAGAAATGGATACCAGCAAGTAATATTTTTTTATTTAAAAATatatagg                                                                                   >  2:2396495/1‑142 (MQ=255)
                                                          cATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTa                                                                                 >  1:1589221/1‑148 (MQ=255)
                                                            tGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGc                                                                               <  1:3504064/148‑1 (MQ=255)
                                                               gAAATGTGATGGTCCATCCGCGGTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGTTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt                                                                            <  2:3212941/148‑1 (MQ=255)
                                                               gAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGtt                                                                            <  1:2096645/148‑1 (MQ=255)
                                                                aaaTGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTc                                                                           <  1:3256515/148‑1 (MQ=255)
                                                                  aTGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTg                                                                         <  1:3064238/148‑1 (MQ=255)
                                                                    gtgATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCg                                                                       >  2:167833/1‑148 (MQ=255)
                                                                      gATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGtt                                                                     >  2:2185221/1‑148 (MQ=255)
                                                                       aTGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTa                                                                    <  2:735241/148‑1 (MQ=255)
                                                                       aTGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTa                                                                    <  2:2245735/148‑1 (MQ=255)
                                                                        tGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTaa                                                                   >  2:829190/1‑148 (MQ=255)
                                                                           tCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGAc                                                                <  1:1168337/148‑1 (MQ=255)
                                                                            ccATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACt                                                               <  1:1742338/148‑1 (MQ=255)
                                                                              aTCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACttt                                                             <  1:2709572/148‑1 (MQ=255)
                                                                               tCCGCGCTGCCGCTGGCTAAACTTGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTAGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtttt                                                            <  1:1150651/148‑1 (MQ=255)
                                                                               tCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtttt                                                            <  2:672972/148‑1 (MQ=255)
                                                                               tCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtttt                                                            >  1:554610/1‑148 (MQ=255)
                                                                               tCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtttt                                                            >  1:554626/1‑148 (MQ=255)
                                                                                ccGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACttttt                                                           <  1:1995765/148‑1 (MQ=255)
                                                                                   cgcTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCa                                                        >  2:2528257/1‑148 (MQ=255)
                                                                                     cTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAtt                                                                                  >  2:8935/1‑120 (MQ=255)
                                                                                         cGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTg                                                  <  2:3087255/148‑1 (MQ=255)
                                                                                         cGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTg                                                  <  2:2445995/148‑1 (MQ=255)
                                                                                              gCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGa                                             >  2:1372760/1‑148 (MQ=255)
                                                                                              gCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGa                                             >  2:2107253/1‑148 (MQ=255)
                                                                                                         aCCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAgg                                  >  2:1029019/1‑148 (MQ=255)
                                                                                                         aCCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAgg                                  <  1:1711950/148‑1 (MQ=255)
                                                                                                         aCCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAATACTTTTTTCATGGGTGCCGGATACAAAAAAgg                                  <  2:1810835/148‑1 (MQ=255)
                                                                                                           cGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGcc                                >  1:253468/1‑148 (MQ=255)
                                                                                                            gTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCg                               >  2:2343806/1‑148 (MQ=255)
                                                                                                               ccTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAg                            >  2:55242/1‑148 (MQ=255)
                                                                                                               ccTCAAGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAg                            >  1:3250735/1‑148 (MQ=255)
                                                                                                                   aaGCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTg                        >  1:1502652/1‑148 (MQ=255)
                                                                                                                     gCAGATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGtt                      <  2:2681673/148‑1 (MQ=255)
                                                                                                                        gATGAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTAcc                   >  2:2647089/1‑148 (MQ=255)
                                                                                                                          tGAAAGCGATTGATTAAATTGTTAAATGTTTCGAAGAACTGGATACAAGAAAGTAATCTTTTTTGCTTGAAAAATATATTATTAGCGTTCTGCGTTAAGACTTTTTTCTTGGGTGCCGGATAAAAAAAAGGCCGCAGGCTGTTAcccc                 <  1:2678774/148‑1 (MQ=255)
                                                                                                                           gAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCt                >  2:384972/1‑148 (MQ=255)
                                                                                                                           gAAAGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCt                <  2:1215300/148‑1 (MQ=255)
                                                                                                                            aaaGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTg               >  2:2778284/1‑148 (MQ=255)
                                                                                                                            aaaGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTg               <  2:3001703/148‑1 (MQ=255)
                                                                                                                             aaGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGc              >  2:1503698/1‑148 (MQ=255)
                                                                                                                             aaGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGc              >  2:377049/1‑148 (MQ=255)
                                                                                                                             aaGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGc              >  2:3120815/1‑148 (MQ=255)
                                                                                                                              aGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCg             >  1:854184/1‑148 (MQ=255)
                                                                                                                              aGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCg             >  1:2247275/1‑148 (MQ=255)
                                                                                                                              aGCGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCg             >  1:853926/1‑148 (MQ=255)
                                                                                                                                cGATTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGNATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGc           >  1:1781324/1‑148 (MQ=255)
                                                                                                                                  aTTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCg         >  2:1395537/1‑148 (MQ=255)
                                                                                                                                  aTTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCg         >  1:1264600/1‑148 (MQ=255)
                                                                                                                                  aTTGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCg         >  1:3175281/1‑148 (MQ=255)
                                                                                                                                   ttGATGATCTGTTTAAAGGTTTCGAAGAACTGGATACAAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTATTAGTTAAGACTTTTTTCATGGTTGCCTGATACAAAAAATGCAGCAGGCTGTTACCCCTGCGGCCgg        >  2:1960136/1‑148 (MQ=255)
                                                                                                                                   ttGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTCTTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCgg        <  1:1454497/148‑1 (MQ=255)
                                                                                                                                   ttGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCgg        >  2:1189407/1‑148 (MQ=255)
                                                                                                                                   ttGATGATCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCgg        >  1:2441347/1‑148 (MQ=255)
                                                                                                                                    tgatgaTCTGGTTAACGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGt       <  1:275101/148‑1 (MQ=255)
                                                                                                                                      atgatCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGttt     >  1:1722875/1‑148 (MQ=255)
                                                                                                                                       ttatCTGGTTAAATGTTTCGAAGAACTGGATACCAGAAAGTAAACTTTTTTGCTTTAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTc    <  1:1347956/146‑1 (MQ=39)
                                                                                                                                       tgatCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTc    <  1:1347912/148‑1 (MQ=255)
                                                                                                                                        gatCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCg   >  2:3321797/1‑148 (MQ=255)
                                                                                                                                         atCTGGTTAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCgg  <  1:2438913/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                            
AGCCGGTATGGCGGTAGGTCTGGCGGGGCTGTTTATTGAAGCGCATCCGGATCCGGAACATGCGAAATGTGATGGTCCATCCGCGCTGCCGCTGGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCATGGGTGCCGGATACAAAAAAGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGG  >  CP009273/1264298‑1264582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑