Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,482,005 G→T 100% D172Y (GAT→TAT)  ydcF → DUF218 superfamily protein, SAM‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,482,0050GT99.5% 686.3 / ‑4.1 209D172Y (GAT→TAT) ydcFDUF218 superfamily protein, SAM‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (96/112);  total (96/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

ACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTAC  >  CP009273/1481861‑1482149
                                                                                                                                                |                                                                                                                                                
acaaACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATg                                                                                                                                               >  1:2273219/1‑148 (MQ=255)
 caaACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGc                                                                                                                                              >  1:2867017/1‑148 (MQ=255)
   aaCTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGcac                                                                                                                                            >  2:782783/1‑148 (MQ=255)
       gCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACg                                                                                                                                        <  1:995203/148‑1 (MQ=255)
          gTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTg                                                                                                                                     >  1:3038494/1‑148 (MQ=255)
            gAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGt                                                                                                                                   >  2:2636510/1‑148 (MQ=255)
            gAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGt                                                                                                                                   >  2:1403759/1‑148 (MQ=255)
              aaaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTa                                                                                                                                 >  2:427027/1‑148 (MQ=255)
              aaaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTa                                                                                                                                 >  2:2194322/1‑148 (MQ=255)
               aaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTaa                                                                                                                                >  2:2409547/1‑148 (MQ=255)
               aaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTaa                                                                                                                                >  2:3002661/1‑148 (MQ=255)
               aaCGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTaa                                                                                                                                >  2:2738111/1‑148 (MQ=255)
                acgcacgcTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAg                                                                                                                               <  1:743808/148‑1 (MQ=255)
                   cacgcTTTATCATCGCGCTATTGAATCAGTCCGTAGAACGAGTTCATACGGCTATCTGTGTTCAGGACCACACAATGCAGCGGCGCACGAAGGCGACGTTCCGCCGTATGACTTGGGACAATCAATATTCACCACGCTGGTTAAGTTa                                                                                                                            >  2:1099464/1‑148 (MQ=255)
                      gcTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATcc                                                                                                                         <  2:3154737/148‑1 (MQ=255)
                         ttAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCgg                                                                                                                      >  1:2368649/1‑148 (MQ=255)
                          tAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGa                                                                                                                     >  2:2117859/1‑148 (MQ=255)
                            gCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGAtt                                                                                                                   <  1:1660470/148‑1 (MQ=255)
                               tCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGt                                                                                                                >  2:1496702/1‑148 (MQ=255)
                                cgcgCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGtt                                                                                                               <  2:1284985/148‑1 (MQ=255)
                                 gcgcTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTc                                                                                                              <  2:1635880/148‑1 (MQ=255)
                                     tATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCa                                                                                                          <  2:2003048/148‑1 (MQ=255)
                                        tGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGtt                                                                                                       <  1:910974/148‑1 (MQ=255)
                                        tGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGGTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGtt                                                                                                       >  1:1999401/1‑148 (MQ=255)
                                            tCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAGTCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGa                                                                                                   >  2:2120560/1‑148 (MQ=255)
                                             cAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGATGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGaa                                                                                                  <  1:895615/148‑1 (MQ=255)
                                              aGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGaaa                                                                                                 <  1:2204675/148‑1 (MQ=255)
                                               ggCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAAt                                                                                                <  2:2943809/148‑1 (MQ=255)
                                                gCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATa                                                                                               <  1:1840936/148‑1 (MQ=255)
                                                gCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATa                                                                                               <  2:1956385/148‑1 (MQ=255)
                                                gCCGTAGAACGAGTTCATACGGCTATCGTAGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATa                                                                                               >  2:847827/1‑148 (MQ=255)
                                                   gTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCNTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACg                                                                                            >  1:1785461/1‑148 (MQ=255)
                                                    tAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGc                                                                                           <  2:3311958/148‑1 (MQ=255)
                                                     aGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCa                                                                                          <  1:61351/148‑1 (MQ=255)
                                                      gAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAg                                                                                         <  2:2192611/148‑1 (MQ=255)
                                                       aaCGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGa                                                                                        >  2:530378/1‑148 (MQ=255)
                                                        aCGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGAc                                                                                       >  1:2148369/1‑148 (MQ=255)
                                                         cGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACa                                                                                      >  2:1885895/1‑148 (MQ=255)
                                                          gAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAg                                                                                     <  1:299261/148‑1 (MQ=255)
                                                           aGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAgt                                                                                    <  2:1946744/148‑1 (MQ=255)
                                                           aGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAgt                                                                                    >  2:1688854/1‑148 (MQ=255)
                                                              tCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTa                                                                                 <  1:3474693/148‑1 (MQ=255)
                                                              tCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTa                                                                                 <  1:3086953/148‑1 (MQ=255)
                                                                  aCGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCt                                                                             >  1:1188573/1‑148 (MQ=255)
                                                                  aCGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCt                                                                             <  2:1700165/148‑1 (MQ=255)
                                                                  aCGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCt                                                                             >  1:1616391/1‑148 (MQ=255)
                                                                    ggCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCttt                                                                           <  1:2664609/148‑1 (MQ=255)
                                                                       tATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTttatt                                                                        >  2:1846495/1‑148 (MQ=255)
                                                                         tCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTaa                                                                      <  2:390387/148‑1 (MQ=255)
                                                                            ttgttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCa                                                                   >  1:1053690/1‑148 (MQ=255)
                                                                             tgttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAg                                                                  <  2:2132272/148‑1 (MQ=255)
                                                                              gttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGt                                                                 <  2:1976089/148‑1 (MQ=255)
                                                                              gttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGt                                                                 <  1:3253800/148‑1 (MQ=255)
                                                                               ttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGtt                                                                <  2:1345868/148‑1 (MQ=255)
                                                                               ttCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGtt                                                                <  1:3180289/148‑1 (MQ=255)
                                                                                tctggACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTa                                                               <  2:1338653/145‑1 (MQ=255)
                                                                                tCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTa                                                               <  2:3085428/148‑1 (MQ=255)
                                                                                tCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTa                                                               >  1:2238297/1‑148 (MQ=255)
                                                                                tCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTa                                                               >  1:3283874/1‑148 (MQ=255)
                                                                                  aGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACa                                                             <  1:2441567/148‑1 (MQ=255)
                                                                                  aGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACa                                                             <  2:2371833/148‑1 (MQ=255)
                                                                                   ggACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACaa                                                            <  2:2258789/148‑1 (MQ=255)
                                                                                     aCCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAgg                                                          >  2:610213/1‑148 (MQ=255)
                                                                                      ccccACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGa                                                         <  1:458320/148‑1 (MQ=255)
                                                                                        ccaccaTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGAtt                                                       <  1:3298865/148‑1 (MQ=255)
                                                                                          accaTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTAt                                                     >  2:1465959/1‑148 (MQ=255)
                                                                                          accaTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTAt                                                     <  2:2427540/148‑1 (MQ=255)
                                                                                            caTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGAAGACAGTGTAATATTTATTAATCAGTTACAAGGAGTATgg                                                   >  1:969717/1‑148 (MQ=255)
                                                                                             aTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGc                                                  >  2:2587298/1‑148 (MQ=255)
                                                                                             aTGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGc                                                  <  1:356753/148‑1 (MQ=255)
                                                                                               gCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCa                                                >  2:2672681/1‑148 (MQ=255)
                                                                                               gCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGGTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCa                                                <  2:3388123/148‑1 (MQ=255)
                                                                                                 aGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGCCAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGt                                              <  2:1925545/148‑1 (MQ=255)
                                                                                                  gcggcgCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGtt                                             <  2:557047/148‑1 (MQ=255)
                                                                                                     gcgcACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAg                                          <  1:2869273/148‑1 (MQ=255)
                                                                                                       gcACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCg                                        <  1:1367285/148‑1 (MQ=255)
                                                                                                       gcACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCg                                        <  1:797294/148‑1 (MQ=255)
                                                                                                        cACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGt                                       >  2:2769515/1‑148 (MQ=255)
                                                                                                         aCGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGtt                                      <  1:3385782/148‑1 (MQ=255)
                                                                                                          cGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTa                                     <  1:575600/148‑1 (MQ=255)
                                                                                                          cGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTa                                     <  1:2117859/148‑1 (MQ=255)
                                                                                                          cGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTa                                     <  1:2741800/148‑1 (MQ=255)
                                                                                                          cGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTa                                     <  1:1275621/148‑1 (MQ=255)
                                                                                                             tGGCGACGGTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAtct                                  <  1:2355110/148‑1 (MQ=255)
                                                                                                               gCGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAtctct                                <  2:2788296/148‑1 (MQ=255)
                                                                                                                cGACGTTCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTAtctctc                               <  1:1470118/148‑1 (MQ=255)
                                                                                                                     ttCCGCCGTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCactac                          >  2:3070629/1‑148 (MQ=255)
                                                                                                                        cgccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGTATTATGGCAAGTTGAGCGTTATCTCTCACTACTCa                       >  1:3184849/1‑148 (MQ=255)
                                                                                                                        cgccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCa                       <  2:2867017/148‑1 (MQ=255)
                                                                                                                          ccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACt                     >  2:1780585/1‑148 (MQ=255)
                                                                                                                          ccgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACt                     >  2:2921152/1‑148 (MQ=255)
                                                                                                                           cgTATGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTg                    >  1:1289271/1‑148 (MQ=255)
                                                                                                                               tGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGa                >  1:1712699/1‑148 (MQ=255)
                                                                                                                               tGACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGa                >  1:3058217/1‑148 (MQ=255)
                                                                                                                                gACTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAg               <  2:1559369/148‑1 (MQ=255)
                                                                                                                                 aCTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGc              >  1:2095045/1‑148 (MQ=255)
                                                                                                                                 aCTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGc              <  1:1292319/148‑1 (MQ=255)
                                                                                                                                 aCTGGGGACAATCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGc              >  1:2094828/1‑148 (MQ=255)
                                                                                                                                          aaTCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATACCGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGtt     <  2:74370/148‑1 (MQ=255)
                                                                                                                                            tCCCTATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTa   >  2:922862/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATTGAATCAGGCCGTAGAACGAGTTCATACGGCTATCGTTGTTCAGGACCCCACCATGCAGCGGCGCACGATGGCGACGTTCCGCCGTATGACTGGGGACAATCCCGATGCACCACGCTGGTTAAGTTATCCCGGATTCGTTCCTCAGTTAGGAAATAACGCAGACAGTGTAATCTTTATTAATCAGTTACAAGGATTATGGCCAGTTGAGCGTTATCTCTCACTACTCACTGGCGAGCTGCCGCGTTTAC  >  CP009273/1481861‑1482149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑