Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,570,428 G→T 100% I483I (ATC→ATA)  yddB ← putative TonB‑dependent outer membrane receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,570,4280GT99.6% 734.5 / ‑4.2 232I483I (ATC→ATA) yddBputative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (129/102);  total (129/103)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

TTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAATGGTATGG  >  CP009273/1570282‑1570572
                                                                                                                                                  |                                                                                                                                                
  aCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAtata                                                                                                                                               <  2:2569343/148‑1 (MQ=255)
   ccTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGatatat                                                                                                                                              >  2:1955148/1‑148 (MQ=255)
      gATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATtgt                                                                                                                                           <  2:614471/148‑1 (MQ=255)
      gATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATtgt                                                                                                                                           >  1:2480495/1‑148 (MQ=255)
      gATACCGATTCCGTCCAGATATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATtgt                                                                                                                                           <  2:2124598/148‑1 (MQ=255)
       aTACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATtgtg                                                                                                                                          <  2:1116566/148‑1 (MQ=255)
       aTACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATtgtg                                                                                                                                          <  1:2264961/148‑1 (MQ=255)
          ccGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGtt                                                                                                                                       <  2:1608918/148‑1 (MQ=255)
          ccGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGtt                                                                                                                                       <  2:2376036/148‑1 (MQ=255)
               tCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACa                                                                                                                                  >  1:3321109/1‑148 (MQ=255)
               tCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACa                                                                                                                                  >  1:1300645/1‑148 (MQ=255)
                        cTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgt                                                                                                                         >  1:1990032/1‑148 (MQ=255)
                        cTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgt                                                                                                                         <  2:1187337/148‑1 (MQ=255)
                        cTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgt                                                                                                                         >  2:903031/1‑148 (MQ=255)
                          aTTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtca                                                                                                                       <  1:2242194/148‑1 (MQ=255)
                           ttGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcat                                                                                                                      <  1:1723889/148‑1 (MQ=255)
                            tGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcata                                                                                                                     >  1:2592686/1‑148 (MQ=255)
                               ggATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTgtcatagtc                                                                                                                  <  2:3300250/148‑1 (MQ=255)
                                gATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGCTATATTGTGGTTTGACAGATAGTTgtcatagtca                                                                                                                 >  2:2769452/1‑148 (MQ=255)
                                      aCGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGc                                                                                                           >  1:730660/1‑148 (MQ=255)
                                         tAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACg                                                                                                        <  1:1657767/148‑1 (MQ=255)
                                         tAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACg                                                                                                        >  2:315742/1‑148 (MQ=255)
                                            tCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCg                                                                                                     >  1:3103490/1‑148 (MQ=255)
                                            tCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTAACAGATAGTTGTCATAGTCATACCGCACGCCg                                                                                                     <  1:1489092/148‑1 (MQ=255)
                                             cccATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCgg                                                                                                    >  1:3140185/1‑148 (MQ=255)
                                                    cAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTaa                                                                                             >  2:2602205/1‑148 (MQ=255)
                                                    cAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTaa                                                                                             >  2:283681/1‑148 (MQ=255)
                                                      aGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATg                                                                                           >  2:357728/1‑148 (MQ=255)
                                                      aGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATg                                                                                           <  1:997849/148‑1 (MQ=255)
                                                        aaTATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGac                                                                                         >  1:3087175/1‑148 (MQ=255)
                                                          tatTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGacac                                                                                       >  1:561989/1‑148 (MQ=255)
                                                           atTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGAcaca                                                                                      <  2:597391/148‑1 (MQ=255)
                                                            ttCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACAt                                                                                     <  2:3464046/148‑1 (MQ=255)
                                                              cccGCCCTAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTa                                                                                   >  2:1322604/1‑148 (MQ=255)
                                                              cccGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTa                                                                                   >  2:1323807/1‑148 (MQ=255)
                                                                  ccATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGcc                                                                               <  2:2949068/148‑1 (MQ=255)
                                                                   cATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCa                                                                              >  2:3066800/1‑148 (MQ=255)
                                                                   cATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCa                                                                              <  2:3124374/148‑1 (MQ=255)
                                                                      aGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCt                                                                           >  1:3171548/1‑148 (MQ=255)
                                                                        tAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTaa                                                                         >  2:547226/1‑148 (MQ=255)
                                                                        tAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTaa                                                                         >  2:548792/1‑148 (MQ=255)
                                                                               tataACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATc                                                                  >  1:693990/1‑148 (MQ=255)
                                                                                 taACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCg                                                                >  1:297682/1‑148 (MQ=255)
                                                                                  aaCCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGc                                                               >  1:2906663/1‑148 (MQ=255)
                                                                                  aaCCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGc                                                               >  1:424235/1‑148 (MQ=255)
                                                                                           tAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAg                                                      <  1:2678115/148‑1 (MQ=255)
                                                                                               cATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCgtg                                                  >  1:911514/1‑148 (MQ=255)
                                                                                               cATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCgtg                                                  >  2:2887312/1‑148 (MQ=255)
                                                                                               cATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCgtg                                                  <  1:1184936/148‑1 (MQ=255)
                                                                                               cATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCgtg                                                  >  2:2887901/1‑148 (MQ=255)
                                                                                                 ttGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTa                                                >  1:184387/1‑148 (MQ=255)
                                                                                                 ttGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTa                                                >  2:3155921/1‑148 (MQ=255)
                                                                                                 ttGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTa                                                >  1:1158021/1‑148 (MQ=255)
                                                                                                  tGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAg                                               >  1:1509097/1‑148 (MQ=255)
                                                                                                  tGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAg                                               <  1:1279079/148‑1 (MQ=255)
                                                                                                        ttGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCa                                         <  2:938862/148‑1 (MQ=255)
                                                                                                        ttGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCa                                         >  1:3347190/1‑148 (MQ=255)
                                                                                                         tGATTAGCAAAAATATCCCAGTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCaa                                        <  2:2762412/148‑1 (MQ=255)
                                                                                                          gATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAAt                                       >  1:2778452/1‑148 (MQ=255)
                                                                                                          gATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAAt                                       >  1:2839364/1‑148 (MQ=255)
                                                                                                           aTTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAAtt                                      >  1:3281840/1‑148 (MQ=255)
                                                                                                            ttAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTc                                     >  1:193559/1‑148 (MQ=255)
                                                                                                             tAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTcc                                    <  2:3293147/148‑1 (MQ=255)
                                                                                                             tAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTcc                                    >  2:3342976/1‑148 (MQ=255)
                                                                                                              aGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTccc                                   >  2:311903/1‑148 (MQ=255)
                                                                                                                cAAAAATATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAg                                 >  1:177965/1‑148 (MQ=255)
                                                                                                                 aaaaaTATCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAgg                                <  2:3131756/148‑1 (MQ=255)
                                                                                                                     atatCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATGAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAggcggc                            <  2:2364495/148‑1 (MQ=255)
                                                                                                                       atCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcct                          <  1:3454877/148‑1 (MQ=255)
                                                                                                                        tCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctt                         >  1:1622165/1‑148 (MQ=255)
                                                                                                                        tCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctt                         >  2:1514141/1‑148 (MQ=255)
                                                                                                                        tCCCATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctt                         <  2:1150929/148‑1 (MQ=255)
                                                                                                                         cccATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGccttt                        <  2:2216381/148‑1 (MQ=255)
                                                                                                                         cccATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGccttt                        >  2:65271/1‑148 (MQ=255)
                                                                                                                           cATTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttac                      <  1:417133/148‑1 (MQ=255)
                                                                                                                            aTTCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacc                     >  2:2450566/1‑148 (MQ=255)
                                                                                                                             ttCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacct                    <  2:2692287/148‑1 (MQ=255)
                                                                                                                             ttCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacct                    >  1:2462556/1‑148 (MQ=255)
                                                                                                                             ttCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacct                    <  2:1896453/148‑1 (MQ=255)
                                                                                                                              tCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacctt                   >  2:3404649/1‑148 (MQ=255)
                                                                                                                              tCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacctt                   >  1:3329164/1‑148 (MQ=255)
                                                                                                                              tCCGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCAGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttacctt                   >  2:2074702/1‑148 (MQ=255)
                                                                                                                               ccGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttaccttt                  <  2:2304548/148‑1 (MQ=255)
                                                                                                                                cGTCATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGcctttaccttta                 >  2:2483098/1‑148 (MQ=255)
                                                                                                                                   cATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATgg              <  2:3103490/148‑1 (MQ=255)
                                                                                                                                   cATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATgg              >  2:1781990/1‑148 (MQ=255)
                                                                                                                                   cATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATgg              >  2:742297/1‑148 (MQ=255)
                                                                                                                                   cATAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATgg              >  1:656378/1‑148 (MQ=255)
                                                                                                                                    aTAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGAGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGt             >  2:946186/1‑148 (MQ=255)
                                                                                                                                     tAAAGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTa            >  2:2487218/1‑148 (MQ=255)
                                                                                                                                       aaGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTaaa          >  1:870612/1‑148 (MQ=255)
                                                                                                                                       aaGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTaaa          >  1:2139555/1‑148 (MQ=255)
                                                                                                                                        aGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAat         <  2:1990032/148‑1 (MQ=255)
                                                                                                                                        aGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAat         <  2:169761/148‑1 (MQ=255)
                                                                                                                                        aGCGCGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAat         <  2:138183/148‑1 (MQ=255)
                                                                                                                                         gcgcGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatg        >  1:1438770/1‑148 (MQ=255)
                                                                                                                                         gcgcGGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatg        <  1:1108814/148‑1 (MQ=255)
                                                                                                                                          cgcgGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatgg       <  1:982203/148‑1 (MQ=255)
                                                                                                                                          cgcgGGGATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatgg       <  2:85219/148‑1 (MQ=255)
                                                                                                                                               ggATATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAatggtatgg  <  2:3324460/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATGACACATTACGCCAGCTAATGCGATCCGCCATATACAGCGTGTAGTTGTCAATTCCCAGGCGGCCTTTACCTTTATGGTAAATGGTATGG  >  CP009273/1570282‑1570572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑