Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,576,325 T→A 100% N153Y (AAT→TAT)  ydeN ← putative Ser‑type periplasmic non‑aryl sulfatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,576,3250TA96.7% 672.1 / 9.6 210N153Y (AAT→TAT) ydeNputative Ser‑type periplasmic non‑aryl sulfatase
Reads supporting (aligned to +/- strand):  ref base T (6/1);  new base A (104/99);  total (110/100)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 214 total aligned reads displayed.

CGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGC  >  CP009273/1576180‑1576469
                                                                                                                                                 |                                                                                                                                                
cgcgTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAgg                                                                                                                                                >  2:2908152/1‑148 (MQ=255)
  cgTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGTCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGaa                                                                                                                                              >  1:437857/1‑148 (MQ=255)
  cgTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGaa                                                                                                                                              >  2:3144230/1‑148 (MQ=255)
  cgTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGaa                                                                                                                                              >  1:1787018/1‑148 (MQ=255)
  cgTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGaa                                                                                                                                              <  2:623654/148‑1 (MQ=255)
      tgtttATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGa                                                                                                                                          >  1:823810/1‑148 (MQ=255)
         ttATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGacac                                                                                                                                       <  2:1738810/148‑1 (MQ=255)
             cTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCaaa                                                                                                                                   >  2:2716184/1‑148 (MQ=255)
             cTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCaaa                                                                                                                                   >  2:2642301/1‑148 (MQ=255)
               tCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAgc                                                                                                                                 >  2:353195/1‑148 (MQ=255)
               tCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAgc                                                                                                                                 >  2:3306305/1‑148 (MQ=255)
                ccGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAgcg                                                                                                                                <  2:3005934/148‑1 (MQ=255)
                    tACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAgcgggcg                                                                                                                            >  2:2263929/1‑148 (MQ=255)
                     aCCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAgcgggcgg                                                                                                                           <  1:1354121/148‑1 (MQ=255)
                        ggCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAg                                                                                                                        <  1:160717/148‑1 (MQ=255)
                          cacaTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCt                                                                                                                      <  1:2980599/148‑1 (MQ=255)
                          cacaTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCt                                                                                                                      <  2:308750/148‑1 (MQ=255)
                          cacaTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCt                                                                                                                      <  1:980428/148‑1 (MQ=255)
                            caTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCg                                                                                                                    >  1:2064226/1‑148 (MQ=255)
                            caTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCg                                                                                                                    >  1:1314311/1‑148 (MQ=255)
                             aTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGa                                                                                                                   >  1:2798248/1‑148 (MQ=255)
                                  tGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGt                                                                                                              >  2:2010881/1‑148 (MQ=255)
                                  tGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGt                                                                                                              >  1:1743365/1‑148 (MQ=255)
                                   gATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTc                                                                                                             >  1:1745109/1‑148 (MQ=255)
                                   gATTTTTGACAAGTGCCATTTACCTACTGCTGAAGTGTAATAACCATTATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGATTAGACACCAAAGCGGGCGGGAGCTCGACCGGTc                                                                                                             <  1:3283133/148‑1 (MQ=255)
                                    aTTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCa                                                                                                            <  2:956599/148‑1 (MQ=255)
                                     tttttGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCat                                                                                                           >  2:1017281/1‑148 (MQ=255)
                                     tttttGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCat                                                                                                           >  2:3435454/1‑148 (MQ=255)
                                     tttttGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCat                                                                                                           <  2:2007859/148‑1 (MQ=255)
                                     tttttGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCat                                                                                                           <  1:1846601/148‑1 (MQ=255)
                                       tttGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCatta                                                                                                         <  1:1644136/148‑1 (MQ=255)
                                         tGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCattatt                                                                                                       <  2:1359789/148‑1 (MQ=255)
                                            cAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTgcg                                                                                                    >  1:2945884/1‑148 (MQ=255)
                                             aaGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTgcgg                                                                                                   <  1:2719809/148‑1 (MQ=255)
                                             aaGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTgcgg                                                                                                   <  1:2172202/148‑1 (MQ=255)
                                              aGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTgcggc                                                                                                  >  1:842339/1‑148 (MQ=255)
                                                tGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGgcgc                                                                                                >  2:3189304/1‑148 (MQ=255)
                                                tGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGgcgc                                                                                                >  2:1443527/1‑148 (MQ=255)
                                                  ccATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCgg                                                                                              >  2:567/1‑148 (MQ=255)
                                                  ccATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCgg                                                                                              >  2:3385882/1‑148 (MQ=255)
                                                  ccATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCgg                                                                                              <  2:2459567/148‑1 (MQ=255)
                                                   cATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCggg                                                                                             >  2:2180575/1‑148 (MQ=255)
                                                      ttACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAgg                                                                                          <  2:140128/148‑1 (MQ=255)
                                                       tACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAggg                                                                                         >  1:2291802/1‑148 (MQ=255)
                                                        aCCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAgggg                                                                                        >  1:3153466/1‑148 (MQ=255)
                                                          cTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGcc                                                                                      >  1:2516696/1‑148 (MQ=255)
                                                           tACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCg                                                                                     >  1:2634437/1‑148 (MQ=255)
                                                           tACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCg                                                                                     >  2:2785836/1‑148 (MQ=255)
                                                               gctgcAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGaaa                                                                                 <  1:1156456/148‑1 (MQ=255)
                                                                ctgcAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAac                                                                                >  2:272741/1‑148 (MQ=255)
                                                                ctgcAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAac                                                                                >  2:272281/1‑148 (MQ=255)
                                                                 tgcAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAaca                                                                               <  2:3104092/148‑1 (MQ=255)
                                                                 tgcAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAaca                                                                               <  2:1848190/148‑1 (MQ=255)
                                                                   cAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCTCGGGAGGGGCCGGAAACAcc                                                                             >  1:1944707/1‑148 (MQ=255)
                                                                    aGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCg                                                                            <  1:3076100/148‑1 (MQ=255)
                                                                      tgtAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCgtg                                                                          <  2:823926/148‑1 (MQ=255)
                                                                        taataaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGtgtg                                                                        >  2:801815/1‑148 (MQ=255)
                                                                         aataaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGc                                                                       >  1:2578008/1‑148 (MQ=255)
                                                                          ataaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGcc                                                                      >  1:1441273/1‑148 (MQ=255)
                                                                           taaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCca                                                                     <  1:1063884/148‑1 (MQ=255)
                                                                           taaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCca                                                                     >  1:2311743/1‑148 (MQ=255)
                                                                           taaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCca                                                                     >  2:2313485/1‑148 (MQ=255)
                                                                             aCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCcaca                                                                   >  1:2019870/1‑148 (MQ=255)
                                                                               cATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATa                                                                 >  2:2294174/1‑148 (MQ=255)
                                                                                 tGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTAGTGCGGCGCGGGCGGGGCCGGAACCACCGTGTGCCACCTAGc                                                               >  1:3349596/1‑148 (MQ=255)
                                                                                    ttCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGt                                                            >  1:1231037/1‑148 (MQ=255)
                                                                                    ttCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGt                                                            <  1:3215787/148‑1 (MQ=255)
                                                                                     tctcGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGtt                                                           >  2:911815/1‑148 (MQ=255)
                                                                                     tCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGtt                                                           <  2:1423097/148‑1 (MQ=255)
                                                                                     tCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGtt                                                           >  1:2998699/1‑148 (MQ=255)
                                                                                           ataatTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTaaa                                                     >  1:3258957/1‑148 (MQ=255)
                                                                                             aatTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAAc                                                   >  2:1319059/1‑148 (MQ=255)
                                                                                             aatTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAAc                                                   >  1:2763687/1‑148 (MQ=255)
                                                                                               ttCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGt                                                 <  1:3424911/148‑1 (MQ=255)
                                                                                               ttCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGt                                                 >  1:1931140/1‑148 (MQ=255)
                                                                                                tCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTa                                                >  1:1799050/1‑148 (MQ=255)
                                                                                                tCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTa                                                <  2:1389713/148‑1 (MQ=255)
                                                                                                tCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTa                                                <  2:1243717/148‑1 (MQ=255)
                                                                                                  aGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACg                                              <  1:689289/148‑1 (MQ=255)
                                                                                                   ggCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGc                                             <  1:3092275/148‑1 (MQ=255)
                                                                                                   ggCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGc                                             >  1:1852173/1‑148 (MQ=255)
                                                                                                       aGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTc                                         <  1:3144223/148‑1 (MQ=255)
                                                                                                       aGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTc                                         >  2:1660631/1‑148 (MQ=255)
                                                                                                        gAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCa                                        >  2:2625668/1‑148 (MQ=255)
                                                                                                          aaGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATc                                      <  1:1428090/148‑1 (MQ=255)
                                                                                                           aGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATcc                                     <  2:1744381/148‑1 (MQ=255)
                                                                                                            gTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCa                                    <  2:2998625/148‑1 (MQ=255)
                                                                                                                  gTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATg                              <  2:2532244/148‑1 (MQ=255)
                                                                                                                    tAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGaa                            <  2:3401050/148‑1 (MQ=255)
                                                                                                                         gAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAg                       <  2:437857/148‑1 (MQ=255)
                                                                                                                         gAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAg                       <  2:285280/148‑1 (MQ=255)
                                                                                                                          aaTACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGc                      <  1:2879671/148‑1 (MQ=255)
                                                                                                                           aTACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCg                     >  2:544436/1‑148 (MQ=255)
                                                                                                                           aTACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCg                     <  1:353195/148‑1 (MQ=255)
                                                                                                                             aCCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTc                   >  1:346478/1‑148 (MQ=255)
                                                                                                                                        cATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGAttt        >  2:333255/1‑148 (MQ=255)
                                                                                                                                           cGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTtg     >  2:283491/1‑148 (MQ=255)
                                                                                                                                           cGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTtg     >  2:3073082/1‑148 (MQ=255)
                                                                                                                                             gTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTtgtg   <  1:567/148‑1 (MQ=255)
                                                                                                                                              tataGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGc  >  2:1442433/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGC  >  CP009273/1576180‑1576469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑