Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,614,837 G→T 100% I186I (ATC→ATA)  eamA ← cysteine and O‑acetyl‑L‑serine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,614,8370GT97.6% 634.9 / 6.0 205I186I (ATC→ATA) eamAcysteine and O‑acetyl‑L‑serine efflux system
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base T (105/95);  total (108/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 205 total aligned reads displayed.

ACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGC  >  CP009273/1614692‑1614981
                                                                                                                                                 |                                                                                                                                                
aCGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTAt                                                                                                                                                <  2:827153/148‑1 (MQ=255)
aCGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTAt                                                                                                                                                <  1:90716/148‑1 (MQ=255)
  gTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTg                                                                                                                                              <  1:272084/148‑1 (MQ=255)
    cccccAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTTATTggg                                                                                                                                            >  2:2522121/1‑148 (MQ=255)
    cccccAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTggg                                                                                                                                            <  2:3292581/148‑1 (MQ=255)
    cccccAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATAAATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTggg                                                                                                                                            <  2:426478/148‑1 (MQ=255)
     ccccAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGa                                                                                                                                           >  2:74733/1‑148 (MQ=255)
        cAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAATGAAGGGAATTATTGGGATTa                                                                                                                                        >  2:3375841/1‑148 (MQ=255)
        cAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTa                                                                                                                                        >  1:793717/1‑148 (MQ=255)
         aGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTaa                                                                                                                                       >  2:2540304/1‑148 (MQ=255)
          gATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTaaa                                                                                                                                      <  2:266332/148‑1 (MQ=255)
           aTCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTTATTGGGATTAAAg                                                                                                                                     >  1:1547980/1‑148 (MQ=255)
            tCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAgc                                                                                                                                    <  2:810483/148‑1 (MQ=255)
               cATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTTATTGGGATTAAAGCGCt                                                                                                                                 >  2:212729/1‑148 (MQ=255)
                 tAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAAGTATTGGGATTAAAGCGCTcc                                                                                                                               >  2:3378024/1‑148 (MQ=255)
                   aCCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAg                                                                                                                             >  1:2168964/1‑148 (MQ=255)
                    ccAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAATGAAGGGAATTATTGGGATTAAAGCGCTCCAGa                                                                                                                            >  2:2462837/1‑148 (MQ=255)
                    ccAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGa                                                                                                                            <  2:2714168/148‑1 (MQ=255)
                    ccAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGa                                                                                                                            >  1:3305102/1‑148 (MQ=255)
                       acaaTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTa                                                                                                                         >  1:2436038/1‑148 (MQ=255)
                        caaTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGAAACAAAGCAGGGAATTATTGGGATTAAAGAGCTCCAGATTAc                                                                                                                        >  2:2100192/1‑148 (MQ=255)
                         aaTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTAcc                                                                                                                       <  1:1715103/148‑1 (MQ=255)
                         aaTTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTAcc                                                                                                                       >  2:1249106/1‑148 (MQ=255)
                                   acaAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCATAATGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTTGGATTAAAGCGATCCAGATTACCAGCGACATCa                                                                                                             <  2:1962118/148‑1 (MQ=255)
                                        cGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCAccgcc                                                                                                        >  1:3240534/1‑148 (MQ=255)
                                               tACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGAcgcg                                                                                                 <  2:162490/148‑1 (MQ=255)
                                                aCATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGt                                                                                                <  2:2173074/148‑1 (MQ=255)
                                                   tCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGa                                                                                             >  1:532922/1‑148 (MQ=255)
                                                     agagACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGt                                                                                           <  1:2951929/148‑1 (MQ=255)
                                                      gagaCAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTg                                                                                          <  1:2646535/148‑1 (MQ=255)
                                                       agaCAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGc                                                                                         >  1:1172517/1‑148 (MQ=255)
                                                        gaCAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCg                                                                                        <  1:3339502/148‑1 (MQ=255)
                                                          cAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGAc                                                                                      >  2:2337940/1‑148 (MQ=255)
                                                          cAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGAc                                                                                      >  1:2222656/1‑148 (MQ=255)
                                                           aaGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACa                                                                                     <  1:2817021/148‑1 (MQ=255)
                                                           aaGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACa                                                                                     >  1:272305/1‑148 (MQ=255)
                                                           aaGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACa                                                                                     <  1:2576195/148‑1 (MQ=255)
                                                             gATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATg                                                                                   >  2:1570563/1‑148 (MQ=255)
                                                             gATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATg                                                                                   >  2:635495/1‑148 (MQ=255)
                                                              aTGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGa                                                                                  <  2:92257/148‑1 (MQ=255)
                                                              aTGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGa                                                                                  <  1:3279182/148‑1 (MQ=255)
                                                                ggtggtCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATc                                                                                <  2:1638855/148‑1 (MQ=255)
                                                                  tggtCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCtt                                                                              <  2:854441/148‑1 (MQ=255)
                                                                  tggtCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCtt                                                                              <  2:2430359/148‑1 (MQ=255)
                                                                    gtCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCtttt                                                                            >  2:785905/1‑148 (MQ=255)
                                                                        tatCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTAtt                                                                        <  2:2548311/148‑1 (MQ=255)
                                                                        tatCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTAtt                                                                        >  1:3391625/1‑148 (MQ=255)
                                                                         atCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTg                                                                       >  2:1468227/1‑148 (MQ=255)
                                                                          tCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGa                                                                      <  1:1973932/148‑1 (MQ=255)
                                                                           cGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGaa                                                                     >  2:181458/1‑148 (MQ=255)
                                                                            gATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAg                                                                    <  1:1878849/148‑1 (MQ=255)
                                                                             aTAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGa                                                                   <  1:761074/148‑1 (MQ=255)
                                                                             aTAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGa                                                                   <  1:2590112/148‑1 (MQ=255)
                                                                               aGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGACTTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATg                                                                 >  1:1621974/1‑148 (MQ=255)
                                                                               aGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATg                                                                 <  1:430183/148‑1 (MQ=255)
                                                                               aGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATg                                                                 <  2:3343542/148‑1 (MQ=255)
                                                                                gTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGt                                                                >  1:818909/1‑148 (MQ=255)
                                                                                   aCCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGc                                                             >  2:425772/1‑148 (MQ=255)
                                                                                    ccAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGcc                                                            >  1:812738/1‑148 (MQ=255)
                                                                                       gACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCcaca                                                         >  2:552303/1‑148 (MQ=255)
                                                                                         cTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACg                                                       <  2:3299302/148‑1 (MQ=255)
                                                                                          tgtgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGc                                                      <  1:884327/148‑1 (MQ=255)
                                                                                            tgAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGccc                                                    >  1:971861/1‑148 (MQ=255)
                                                                                                tCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACt                                                >  1:855976/1‑148 (MQ=255)
                                                                                                  aTGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTaa                                              <  1:2411136/148‑1 (MQ=255)
                                                                                                  aTGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTaa                                              <  2:1524195/148‑1 (MQ=255)
                                                                                                      ttGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtgc                                          <  1:1808801/148‑1 (MQ=255)
                                                                                                       tGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtgct                                         >  1:2024091/1‑148 (MQ=255)
                                                                                                         cGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAAtgctgc                                       <  1:805018/148‑1 (MQ=255)
                                                                                                              ccATCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCa                                  <  2:385985/148‑1 (MQ=255)
                                                                                                                 tCGAGAATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAggg                               >  1:2203462/1‑148 (MQ=255)
                                                                                                                     gaATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCaa                           <  1:667829/148‑1 (MQ=255)
                                                                                                                     gaATCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCaa                           <  1:1468227/148‑1 (MQ=255)
                                                                                                                      aaTCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAAc                          <  2:2315708/148‑1 (MQ=255)
                                                                                                                        tCAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCAACACGCCCAACTAAATGCTGCCGCCAGGGTCAACAt                        >  2:1080339/1‑148 (MQ=255)
                                                                                                                         cAGCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATa                       >  1:1682799/1‑148 (MQ=255)
                                                                                                                         cAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATa                       >  1:1356757/1‑148 (MQ=255)
                                                                                                                           gCGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATaaa                     <  2:2852814/148‑1 (MQ=255)
                                                                                                                            cGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAg                    >  2:21624/1‑148 (MQ=255)
                                                                                                                            cGAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAg                    >  2:423473/1‑148 (MQ=255)
                                                                                                                             gAGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGc                   <  2:2797275/148‑1 (MQ=255)
                                                                                                                              aGGCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGcc                  >  1:122644/1‑148 (MQ=255)
                                                                                                                               ggCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCg                 >  1:2203158/1‑148 (MQ=255)
                                                                                                                                gCAACAAAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGa                >  1:490445/1‑148 (MQ=255)
                                                                                                                                  aacaaAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGc              >  2:2356061/1‑148 (MQ=255)
                                                                                                                                   acaaAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCa             >  2:2951189/1‑148 (MQ=255)
                                                                                                                                    caaAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCAt            <  2:2436038/148‑1 (MQ=255)
                                                                                                                                    caaAGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCAt            <  1:3107336/148‑1 (MQ=255)
                                                                                                                                     aaaGAAGGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATc           >  1:2769192/1‑148 (MQ=255)
                                                                                                                                        gaagGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGcc        >  1:2316146/1‑148 (MQ=255)
                                                                                                                                        gaagGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGcc        <  1:275045/148‑1 (MQ=255)
                                                                                                                                        gaagGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGcc        >  2:1922384/1‑148 (MQ=255)
                                                                                                                                         aagGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCca       >  1:65638/1‑148 (MQ=255)
                                                                                                                                          agGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCcac      >  2:482110/1‑148 (MQ=255)
                                                                                                                                          agGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCcac      <  2:2237273/148‑1 (MQ=255)
                                                                                                                                          agGGAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCcac      <  1:3183811/148‑1 (MQ=255)
                                                                                                                                           gggAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCcaca     >  1:109167/1‑148 (MQ=255)
                                                                                                                                             gAATTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATg   <  2:598096/148‑1 (MQ=255)
                                                                                                                                              aaTTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGc  <  2:3454630/148‑1 (MQ=255)
                                                                                                                                              aaTTATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGc  <  1:599654/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACGTCCCCCAGATCCCATAACCAACAATTGTCGCCACAAACGCCAGATACATCAGAGACAAGATGGTGGTCATATCGATAGTAACCAGACTGTGAATCATGGTTGCGGAACCATCGAGAATCAGCGAGGCAACAAAGAAGGGAATGATTGGGATTAAAGCGCTCCAGATTACCAGCGACATCACCGCCGGACGCGTTGAGTGCGACATGATCTTTTTATTGAAGATGTTGCCACACGCCCAACTAAATGCTGCCGCCAGGGTCAACATAAAGCCGAGCATCGCCACATGC  >  CP009273/1614692‑1614981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑