Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,666,805 A→C 94.3% S197R (AGT→CGT)  ydgD → putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,666,8050AC94.3% 846.1 / 17.9 225S197R (AGT→CGT) ydgDputative peptidase
Reads supporting (aligned to +/- strand):  ref base A (7/6);  new base C (123/89);  total (130/95)
Fisher's exact test for biased strand distribution p-value = 7.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 225 total aligned reads displayed.

ATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTC  >  CP009273/1666661‑1666949
                                                                                                                                                |                                                                                                                                                
 ttgTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGtca                                                                                                                                              >  2:2599019/1‑148 (MQ=255)
  tgtgCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGtcat                                                                                                                                             >  1:2940993/1‑148 (MQ=255)
  tgtgCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGtcat                                                                                                                                             <  2:1042804/148‑1 (MQ=255)
      cTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCaaa                                                                                                                                         <  2:912755/148‑1 (MQ=255)
       tACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCaaaa                                                                                                                                        >  1:2561470/1‑148 (MQ=255)
         cGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACt                                                                                                                                      >  2:1598778/1‑148 (MQ=255)
          gTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACtg                                                                                                                                     >  1:1326092/1‑148 (MQ=255)
           tAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACtgt                                                                                                                                    <  2:784525/148‑1 (MQ=255)
             aTCCCCCTTCGGGCATTACGCCGTTGCCGTTATTTGAGGTAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGa                                                                                                                                  <  2:17251/148‑1 (MQ=255)
              tCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGaa                                                                                                                                 >  2:264744/1‑148 (MQ=255)
               cccccTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAg                                                                                                                                >  2:565217/1‑148 (MQ=255)
                 cccTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTg                                                                                                                              <  2:2535298/148‑1 (MQ=255)
                 cccTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTg                                                                                                                              <  1:888966/148‑1 (MQ=255)
                  ccTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGa                                                                                                                             >  2:1276540/1‑148 (MQ=255)
                  ccTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGa                                                                                                                             >  2:3467826/1‑148 (MQ=255)
                   cTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGAc                                                                                                                            >  2:1846165/1‑148 (MQ=255)
                    ttCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAATTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGAct                                                                                                                           >  1:3431959/1‑148 (MQ=255)
                     tCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGActg                                                                                                                          >  1:1731914/1‑148 (MQ=255)
                     tCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGActg                                                                                                                          >  1:2406061/1‑148 (MQ=255)
                      cTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGActgg                                                                                                                         >  1:3000075/1‑148 (MQ=255)
                        ggCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGActggct                                                                                                                       >  1:3503483/1‑148 (MQ=255)
                        ggCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGActggct                                                                                                                       <  1:2744812/148‑1 (MQ=255)
                         gCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGActggctg                                                                                                                      >  1:2856214/1‑148 (MQ=255)
                           aTTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTggg                                                                                                                    >  2:2783772/1‑148 (MQ=255)
                             tACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGgcg                                                                                                                  >  1:3164735/1‑148 (MQ=255)
                             tACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGgcg                                                                                                                  >  2:2205722/1‑148 (MQ=255)
                                gccgttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCaa                                                                                                               <  1:1600674/148‑1 (MQ=255)
                                gccgttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCaa                                                                                                               <  1:1048521/148‑1 (MQ=255)
                                   gttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCGACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACg                                                                                                            <  2:135589/148‑1 (MQ=255)
                                   gttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCGACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACg                                                                                                            <  2:1908707/148‑1 (MQ=255)
                                    ttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGt                                                                                                           <  1:511959/148‑1 (MQ=255)
                                    ttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGt                                                                                                           <  1:511474/148‑1 (MQ=255)
                                     tgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTc                                                                                                          >  1:1461362/1‑148 (MQ=255)
                                       ccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCgg                                                                                                        >  2:430173/1‑148 (MQ=255)
                                       ccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCgg                                                                                                        >  2:1720653/1‑148 (MQ=255)
                                       ccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAAAGTCgg                                                                                                        >  1:2277737/1‑148 (MQ=255)
                                         gttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTg                                                                                                      >  2:2228092/1‑148 (MQ=255)
                                           tATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGAt                                                                                                    <  2:2676948/148‑1 (MQ=255)
                                             tttGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGt                                                                                                  >  1:177324/1‑148 (MQ=255)
                                                      gaTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGt                                                                                                  <  2:2318162/139‑1 (MQ=255)
                                                      gaTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGt                                                                                         >  1:994612/1‑148 (MQ=255)
                                                       aTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTg                                                                                        >  1:2685754/1‑148 (MQ=255)
                                                       aTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTg                                                                                        >  1:877631/1‑148 (MQ=255)
                                                       aTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACAGCAGTg                                                                                        >  2:537312/1‑148 (MQ=255)
                                                       aTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGAAACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTg                                                                                        >  2:1132364/1‑148 (MQ=255)
                                                        tAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGc                                                                                       <  1:2802652/148‑1 (MQ=255)
                                                        tAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGc                                                                                       >  2:1322141/1‑148 (MQ=255)
                                                          aaGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGa                                                                                     >  2:2488006/1‑148 (MQ=255)
                                                           aGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGAt                                                                                    >  1:622094/1‑148 (MQ=255)
                                                           aGCCGCGCTAACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGAt                                                                                    >  2:3287197/1‑148 (MQ=255)
                                                              cgcgCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATAcc                                                                                 <  2:2077061/148‑1 (MQ=255)
                                                              cgcgCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATAcc                                                                                 <  2:3200405/148‑1 (MQ=255)
                                                                 gcTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTg                                                                              <  1:2671138/148‑1 (MQ=255)
                                                                    tACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCg                                                                           <  1:2402973/148‑1 (MQ=255)
                                                                     aCCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCgg                                                                          >  2:1215591/1‑148 (MQ=255)
                                                                        gccgcATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTg                                                                       <  2:1059977/148‑1 (MQ=255)
                                                                        gccgcATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTg                                                                       <  2:3251371/148‑1 (MQ=255)
                                                                          cgcATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGAc                                                                     >  1:1965927/1‑148 (MQ=255)
                                                                          cgcATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGAc                                                                     <  2:3423055/148‑1 (MQ=255)
                                                                               tAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATAACTTGCCGGGTGACAGCgg                                                                >  2:3086874/1‑148 (MQ=255)
                                                                                aaaaGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGt                                                               <  1:114775/148‑1 (MQ=255)
                                                                                aaaaGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGt                                                               >  2:1963862/1‑148 (MQ=255)
                                                                                 aaaGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGtt                                                              <  1:2064411/148‑1 (MQ=255)
                                                                                  aaGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTc                                                             >  1:2734880/1‑148 (MQ=255)
                                                                                     cGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGcc                                                          >  2:829794/1‑148 (MQ=255)
                                                                                      ggCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCct                                                         <  1:840847/148‑1 (MQ=255)
                                                                                       gCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCctc                                                        <  2:3142246/148‑1 (MQ=255)
                                                                                       gCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCctc                                                        <  2:355618/148‑1 (MQ=255)
                                                                                        cAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCctct                                                       >  2:981375/1‑148 (MQ=255)
                                                                                           gTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGAt                                                    >  1:11567/1‑148 (MQ=255)
                                                                                            tCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCAACAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATg                                                   >  1:1996165/1‑148 (MQ=255)
                                                                                                  aGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCAt                                             <  2:2421936/148‑1 (MQ=255)
                                                                                                         caggcaggCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATg                                      <  2:1516789/148‑1 (MQ=255)
                                                                                                         caggcaggCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATg                                      <  1:2139781/148‑1 (MQ=255)
                                                                                                         caggcaggCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATg                                      >  1:3335768/1‑148 (MQ=255)
                                                                                                         caggcaggCTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATg                                      <  1:2907657/148‑1 (MQ=255)
                                                                                                             caggcTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGGTCGCCTCTGATGTTGCATACCGATGACgg                                  <  2:1787059/148‑1 (MQ=255)
                                                                                                                gcTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTg                               >  1:1870586/1‑148 (MQ=255)
                                                                                                                gcTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTg                               >  2:2510640/1‑148 (MQ=255)
                                                                                                                gcTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTg                               >  1:1711457/1‑148 (MQ=255)
                                                                                                                gcTACCCTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTg                               <  2:2918822/148‑1 (MQ=255)
                                                                                                                    cccTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCaa                           >  2:2296671/1‑148 (MQ=255)
                                                                                                                    cccTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCaa                           <  2:881840/148‑1 (MQ=255)
                                                                                                                    cccTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCaa                           <  1:2783772/148‑1 (MQ=255)
                                                                                                                    cccTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCaa                           <  1:710920/148‑1 (MQ=255)
                                                                                                                     ccTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCaat                          >  1:868829/1‑148 (MQ=255)
                                                                                                                     ccTGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCaat                          >  2:481667/1‑148 (MQ=255)
                                                                                                                     ccTGAAGATCATCTCGATACGTTGTAACGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACTACGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCaat                          >  1:2227543/1‑148 (MQ=255)
                                                                                                                       tGAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCaatta                        >  2:618997/1‑148 (MQ=255)
                                                                                                                        gAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCaattaa                       <  2:2190754/148‑1 (MQ=255)
                                                                                                                        gAAGATCATCTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCaattaa                       <  2:2886438/148‑1 (MQ=255)
                                                                                                                         aaGATCATCTCGATACGTTGTTCCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGTCaattaat                      <  1:2477304/148‑1 (MQ=255)
                                                                                                                            atcatcTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTgg                   >  2:1321301/1‑148 (MQ=255)
                                                                                                                              catcTCGATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTgggg                 <  1:2567866/148‑1 (MQ=255)
                                                                                                                                    gATACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCaaa           <  1:2441553/148‑1 (MQ=255)
                                                                                                                                     aTACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGCTGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAg          <  1:1798540/148‑1 (MQ=255)
                                                                                                                                     aTACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAg          >  2:1456738/1‑148 (MQ=255)
                                                                                                                                      tACGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGt         >  1:2184400/1‑148 (MQ=255)
                                                                                                                                        cGTTGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTc       >  1:1146376/1‑148 (MQ=255)
                                                                                                                                          ttGTACCGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCgg     >  1:1582683/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ATTGTGCTACGTAATCCCCCTTCTGGCATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTGTGAAGTGACTGGCTGGGCGCAAACGTCGGTGATGTCACATCAGTGCGATACCTTGCCGGGTGACAGCGGTTCGCCTCTGATGTTGCATACCGATGACGGCTGGCAATTAATTGGGGTGCAAAGTTCGGCTC  >  CP009273/1666661‑1666949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑