Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,723,809 G→T 100% E156* (GAA→TAA)  lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,723,8090GT99.0% 593.9 / ‑3.2 188E156* (GAA→TAA) lhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (99/87);  total (101/87)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 188 total aligned reads displayed.

CCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTG  >  CP009273/1723664‑1723952
                                                                                                                                                 |                                                                                                                                               
cccGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTaa                                                                                                                                               <  2:3273717/148‑1 (MQ=255)
 ccGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAAt                                                                                                                                              >  1:2795955/1‑148 (MQ=255)
  cGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATc                                                                                                                                             <  2:1379164/148‑1 (MQ=255)
     tGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCAct                                                                                                                                          >  2:392482/1‑148 (MQ=255)
       aaGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCActct                                                                                                                                        <  1:617304/148‑1 (MQ=255)
        aGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTa                                                                                                                                       <  2:2374138/148‑1 (MQ=255)
         gggTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTAt                                                                                                                                      >  2:553752/1‑148 (MQ=255)
             aTTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGa                                                                                                                                  <  2:2604721/148‑1 (MQ=255)
              ttGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGAt                                                                                                                                 <  1:751875/148‑1 (MQ=255)
              ttGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGAt                                                                                                                                 <  1:2513286/148‑1 (MQ=255)
              ttGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGAGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGAt                                                                                                                                 >  1:3013093/1‑148 (MQ=255)
               tGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATg                                                                                                                                >  2:1995569/1‑148 (MQ=255)
                 ccGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCt                                                                                                                              <  1:1390866/148‑1 (MQ=255)
                  cGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTg                                                                                                                             <  1:2461513/148‑1 (MQ=255)
                  cGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTg                                                                                                                             <  2:331232/148‑1 (MQ=255)
                   gATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGa                                                                                                                            >  1:2884755/1‑148 (MQ=255)
                     tGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGAcc                                                                                                                          <  2:1780789/148‑1 (MQ=255)
                        aCGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTcc                                                                                                                       <  1:3243480/148‑1 (MQ=255)
                        aCGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTcc                                                                                                                       <  1:925300/148‑1 (MQ=255)
                                   gcggcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGa                                                                                                            <  1:320145/148‑1 (MQ=255)
                                       cgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACg                                                                                                        >  1:2751402/1‑148 (MQ=255)
                                        gAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGc                                                                                                       <  1:3187718/148‑1 (MQ=255)
                                         aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCt                                                                                                      >  1:3429490/1‑148 (MQ=255)
                                         aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCt                                                                                                      >  1:2676809/1‑148 (MQ=255)
                                          aaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTa                                                                                                     >  1:1578484/1‑148 (MQ=255)
                                          aaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTa                                                                                                     <  2:2211195/148‑1 (MQ=255)
                                              gAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAcgcg                                                                                                 >  1:2290191/1‑148 (MQ=255)
                                               aaGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcgg                                                                                                <  1:504116/148‑1 (MQ=255)
                                                 gTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcggcg                                                                                              >  1:3371961/1‑148 (MQ=255)
                                                 gTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcggcg                                                                                              <  2:2501352/148‑1 (MQ=255)
                                                 gTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcggcg                                                                                              <  2:2501613/148‑1 (MQ=255)
                                                  tCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGt                                                                                             <  1:3268801/148‑1 (MQ=255)
                                                      tCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGaa                                                                                         >  1:1794625/1‑148 (MQ=255)
                                                          cgcgTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACgg                                                                                     >  2:2768649/1‑148 (MQ=255)
                                                             gTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTaa                                                                                  <  1:65471/148‑1 (MQ=255)
                                                              tAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAat                                                                                 >  1:2520070/1‑148 (MQ=255)
                                                               aGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAatt                                                                                >  2:2899878/1‑148 (MQ=255)
                                                                gggATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTAGCTGATGCTGACCTCCCGCGCGCGCGAGACGCTACGCGGCGTCGATACGGTCatta                                                                               >  2:1675445/1‑148 (MQ=255)
                                                                 ggATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAattat                                                                              >  2:3095639/1‑148 (MQ=255)
                                                                 ggATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAagtat                                                                              >  2:3095905/1‑148 (MQ=255)
                                                                   aTCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTCTtg                                                                            >  2:2463515/1‑148 (MQ=255)
                                                                   aTCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtg                                                                            >  2:336512/1‑148 (MQ=255)
                                                                     ccGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtgat                                                                          >  1:1174119/1‑148 (MQ=255)
                                                                       gTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTc                                                                                            <  1:202329/128‑1 (MQ=255)
                                                                        tACTGGCGATACGCCTGCACAGGAACGCAGCAACCTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGaa                                                                       <  2:1696297/148‑1 (MQ=255)
                                                                        tACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGaa                                                                       >  2:3446328/1‑148 (MQ=255)
                                                                          cTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGACACGGTCCGTATTGATGAAGt                                                                     >  2:2120899/1‑148 (MQ=255)
                                                                          cTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGt                                                                     <  2:596919/148‑1 (MQ=255)
                                                                                 tACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCAcgcg                                                              >  2:1590713/1‑148 (MQ=255)
                                                                                     ccTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGACACGCTACGCGGCGTCGAAACGGTAAGTATTGATGAAGTCCACGCGGTAg                                                          >  2:3418883/1‑148 (MQ=255)
                                                                                     ccTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAg                                                          <  1:1054420/148‑1 (MQ=255)
                                                                                           caGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCa                                                    >  2:408259/1‑148 (MQ=255)
                                                                                           caGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCa                                                    >  1:1208625/1‑148 (MQ=255)
                                                                                              gAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTa                                                 >  1:380937/1‑148 (MQ=255)
                                                                                               aaCGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTACTCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTaa                                                <  1:2307824/148‑1 (MQ=255)
                                                                                               aaCGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTaa                                                <  1:2686272/148‑1 (MQ=255)
                                                                                                 cGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAAc                                              >  1:2487194/1‑148 (MQ=255)
                                                                                                      caAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtggt                                         >  1:1461973/1‑148 (MQ=255)
                                                                                                        aaCTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTgc                                       >  1:2046460/1‑148 (MQ=255)
                                                                                                         aCTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTgcg                                      >  2:1556850/1‑148 (MQ=255)
                                                                                                            cACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCAt                                   >  1:890336/1‑148 (MQ=255)
                                                                                                            cACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCAt                                   <  1:985709/148‑1 (MQ=255)
                                                                                                            cACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCAt                                   >  1:890344/1‑148 (MQ=255)
                                                                                                             aCCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATc                                  <  2:979091/148‑1 (MQ=255)
                                                                                                             aCCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATc                                  >  1:2147994/1‑148 (MQ=255)
                                                                                                             aCCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATc                                  >  1:290818/1‑148 (MQ=255)
                                                                                                             aCCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATc                                  >  1:1423903/1‑148 (MQ=255)
                                                                                                                cGTAATCCGCCGGATATTCTGATCACCACACCCTCAGCACTCTATCTTATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGACGTCCACGGGGTAGCGGGCAGTAATAGTGGTGCGCATCTgg                               <  2:3013093/148‑1 (MQ=255)
                                                                                                                cGTAATCCGCCGGATATTCTGATCACCACACCCTACTCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTgg                               <  1:3365507/148‑1 (MQ=255)
                                                                                                                   aaTCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGt                            <  1:2594083/148‑1 (MQ=255)
                                                                                                                      ccgccgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTaa                         <  1:1733576/148‑1 (MQ=255)
                                                                                                                      ccgccgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTaa                         >  1:2614154/1‑148 (MQ=255)
                                                                                                                         ccgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTc                      <  1:156987/148‑1 (MQ=255)
                                                                                                                          cgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCt                     <  1:1211330/148‑1 (MQ=255)
                                                                                                                             atatTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGACACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGa                  >  2:1079943/1‑148 (MQ=255)
                                                                                                                             atatTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGa                  >  2:794416/1‑148 (MQ=255)
                                                                                                                              tatTCTGATCACCACACCCTAATCACTCTCTCTGAGGCTGACCTCCCGCGCGCGCGAAACGCGACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAg                 <  2:3471618/148‑1 (MQ=255)
                                                                                                                               atTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGc                >  1:1028476/1‑148 (MQ=255)
                                                                                                                                ttCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCg               >  1:1640405/1‑148 (MQ=255)
                                                                                                                                 tCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCgg              >  2:3502893/1‑148 (MQ=255)
                                                                                                                                 tCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCgg              >  1:1856894/1‑148 (MQ=255)
                                                                                                                                 tCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCgg              <  2:2461345/148‑1 (MQ=255)
                                                                                                                                  cTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGc             >  2:1192936/1‑148 (MQ=255)
                                                                                                                                  cTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGc             <  2:1973283/148‑1 (MQ=255)
                                                                                                                                  cTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGc             <  1:1995569/148‑1 (MQ=255)
                                                                                                                                      tCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGa         >  1:226586/1‑148 (MQ=255)
                                                                                                                                      tCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGa         <  1:1228867/148‑1 (MQ=255)
                                                                                                                                      tCACCACACCCTAATCACTCTATCTGATGCTGACCGCCCGCGCGCGCGACCCGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGa         <  1:947979/148‑1 (MQ=255)
                                                                                                                                       caccacACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGAt        >  2:2830210/1‑148 (MQ=255)
                                                                                                                                       caccacACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGAt        >  2:1315826/1‑148 (MQ=255)
                                                                                                                                         ccacACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATgc      >  2:3141191/1‑148 (MQ=255)
                                                                                                                                         ccacACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATgc      >  2:1392808/1‑148 (MQ=255)
                                                                                                                                           acacCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATgcgc    >  1:1757792/1‑148 (MQ=255)
                                                                                                                                           acacCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATgcgc    <  2:685919/148‑1 (MQ=255)
                                                                                                                                            cacCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgct   >  1:492493/1‑148 (MQ=255)
                                                                                                                                            cacCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgct   >  2:2184627/1‑148 (MQ=255)
                                                                                                                                            cacCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGAGGCgct   >  2:2796485/1‑148 (MQ=255)
                                                                                                                                            cacCCTAATCACTCTATCAGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgct   >  2:1418855/1‑148 (MQ=255)
                                                                                                                                             acCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgctg  <  1:3125634/148‑1 (MQ=255)
                                                                                                                                             acCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgctg  >  2:2024814/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTG  >  CP009273/1723664‑1723952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑