Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,735,082 G→T 100% V305V (GTG→GTT)  ydhC → putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,735,0820GT95.0% 656.2 / 22.8 222V305V (GTG→GTT) ydhCputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (7/4);  new base T (102/108);  total (110/112)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 226 total aligned reads displayed.

TGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTGGC  >  CP009273/1734935‑1735227
                                                                                                                                                   |                                                                                                                                                 
  gCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTAt                                                                                                                                                 <  1:3269973/148‑1 (MQ=255)
   cTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATg                                                                                                                                                >  1:1674722/1‑148 (MQ=255)
    tGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATgg                                                                                                                                               <  1:1600986/148‑1 (MQ=255)
      tCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCg                                                                                                                                             <  2:2855964/148‑1 (MQ=255)
       cgcgccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGa                                                                                                                                            <  2:1240604/148‑1 (MQ=255)
          gccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTg                                                                                                                                         <  2:146839/148‑1 (MQ=255)
           ccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGc                                                                                                                                        <  2:1917080/148‑1 (MQ=255)
           ccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGc                                                                                                                                        >  2:3350097/1‑148 (MQ=255)
           ccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGc                                                                                                                                        <  2:698696/148‑1 (MQ=255)
           ccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGc                                                                                                                                        <  2:698978/148‑1 (MQ=255)
              cgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCaa                                                                                                                                     <  2:789536/148‑1 (MQ=255)
               gcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAAt                                                                                                                                    <  1:2084511/148‑1 (MQ=255)
               gcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAAt                                                                                                                                    <  2:2791924/148‑1 (MQ=255)
                cTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATg                                                                                                                                   <  1:1502374/148‑1 (MQ=255)
                 tGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATgg                                                                                                                                  <  1:3411756/148‑1 (MQ=255)
                   cAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGgcg                                                                                                                                >  2:2096361/1‑148 (MQ=255)
                   cAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGgcg                                                                                                                                >  1:2189214/1‑148 (MQ=255)
                      aaaTGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGa                                                                                                                             >  2:308921/1‑148 (MQ=255)
                       aaTGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGAt                                                                                                                            <  1:2278502/148‑1 (MQ=255)
                         tGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCt                                                                                                                          >  2:2737843/1‑148 (MQ=255)
                         tGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCt                                                                                                                          >  2:1774225/1‑148 (MQ=255)
                               ggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGTGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTa                                                                                                                    <  1:283718/148‑1 (MQ=255)
                               ggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTa                                                                                                                    >  2:3392615/1‑148 (MQ=255)
                               ggcaagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTa                                                                                                                    >  2:2548208/1‑148 (MQ=255)
                                  aagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAttg                                                                                                                 <  1:1030306/148‑1 (MQ=255)
                                  aagCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAttg                                                                                                                 <  2:2640978/148‑1 (MQ=255)
                                    gCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAttgtt                                                                                                               >  2:3451811/1‑148 (MQ=255)
                                     cAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAttgttg                                                                                                              >  1:1842889/1‑148 (MQ=255)
                                     cAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTAttgttg                                                                                                              >  1:2489715/1‑148 (MQ=255)
                                        ttattaCCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCg                                                                                                           >  1:1236596/1‑148 (MQ=255)
                                          attaCCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGcc                                                                                                         >  2:2883582/1‑148 (MQ=255)
                                             aCCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAg                                                                                                      <  2:3044256/148‑1 (MQ=255)
                                                gTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGgcg                                                                                                   <  1:60658/148‑1 (MQ=255)
                                                gTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGgcg                                                                                                   <  1:2580950/148‑1 (MQ=255)
                                                gTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGgcg                                                                                                   >  2:2901596/1‑148 (MQ=255)
                                                 tGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGgcgc                                                                                                  >  2:2383940/1‑148 (MQ=255)
                                                   gTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTg                                                                                                <  1:1848087/148‑1 (MQ=255)
                                                         ggTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTccc                                                                                          <  2:3263985/148‑1 (MQ=255)
                                                              tGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTccc                                                                                     <  2:202294/148‑1 (MQ=255)
                                                              tGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTccc                                                                                     <  2:201939/148‑1 (MQ=255)
                                                                       tCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAAc                                                                            >  1:221930/1‑148 (MQ=255)
                                                                         aGCGTCATAGCGACCTGGGCTGCGGGAATCATTAGCCAACATTCGCTGGTCGAAATCCTGATCCCATTCTGTGTAATGGCCATTGCCAATGGCGCGATCTACACTATTGTTGTCGCCCAGGCGCTGCTTCCCTTACCACACGCAACTg                                                                          >  2:3502888/1‑148 (MQ=255)
                                                                          gCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTgg                                                                         <  1:3204342/148‑1 (MQ=255)
                                                                          gCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTgg                                                                         <  2:2104995/148‑1 (MQ=255)
                                                                             tCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTcg                                                                      >  1:2558576/1‑148 (MQ=255)
                                                                             tCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTcg                                                                      >  1:945598/1‑148 (MQ=255)
                                                                                     aCCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAg                                                              <  1:613663/148‑1 (MQ=255)
                                                                                      ccTGGGCTGCGGGCTTCATTAGCCATGTGTCTCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGc                                                             >  1:1935847/1‑148 (MQ=255)
                                                                                      ccTGGGCTGCGGGCTTCATTAGCCATGTGTCTCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGc                                                             >  1:2334256/1‑148 (MQ=255)
                                                                                      ccTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGc                                                             >  2:2553695/1‑148 (MQ=255)
                                                                                       cTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCg                                                            <  2:1888028/148‑1 (MQ=255)
                                                                                        tGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGt                                                           <  1:284826/148‑1 (MQ=255)
                                                                                        tGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGt                                                           >  2:811053/1‑148 (MQ=255)
                                                                                          ggCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTg                                                         <  2:1789303/148‑1 (MQ=255)
                                                                                          ggCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTg                                                         >  1:803213/1‑148 (MQ=255)
                                                                                                gggCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGCTCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAac                                                   <  2:1711845/148‑1 (MQ=255)
                                                                                                 ggCTTCATTAGCCATGTGTCGCTTGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCTCACGCAACTGGTCGCGCCGCAGCGTTGCAGAaca                                                  <  2:3265221/148‑1 (MQ=255)
                                                                                                 ggCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAaca                                                  <  2:3098572/148‑1 (MQ=255)
                                                                                                  gCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAacac                                                 >  2:212392/1‑148 (MQ=255)
                                                                                                    ttCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACActc                                               >  2:3134281/1‑148 (MQ=255)
                                                                                                    ttCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACActc                                               <  1:749753/148‑1 (MQ=255)
                                                                                                     tCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACActct                                              >  2:1345024/1‑148 (MQ=255)
                                                                                                       aTTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTc                                            <  2:1107302/148‑1 (MQ=255)
                                                                                                           gCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACt                                        <  1:2855354/148‑1 (MQ=255)
                                                                                                            ccATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTg                                       >  1:2407383/1‑148 (MQ=255)
                                                                                                              aTGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTggg                                     <  1:725003/148‑1 (MQ=255)
                                                                                                                gtgtCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTc                                   >  2:1040575/1‑148 (MQ=255)
                                                                                                                 tgtCGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCt                                  >  1:3073207/1‑148 (MQ=255)
                                                                                                                    cGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCtgtg                               <  2:2157155/148‑1 (MQ=255)
                                                                                                                    cGCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCtgtg                               <  1:2028723/148‑1 (MQ=255)
                                                                                                                     gCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGc                              <  2:1842933/148‑1 (MQ=255)
                                                                                                                     gCTGGTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGc                              <  2:804425/148‑1 (MQ=255)
                                                                                                                         gTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTcc                          >  1:1001295/1‑148 (MQ=255)
                                                                                                                         gTCGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTcc                          >  2:2824605/1‑148 (MQ=255)
                                                                                                                           cGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAAc                                         <  1:247631/131‑1 (MQ=255)
                                                                                                                           cGAAATCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAAc                                         >  2:247631/1‑131 (MQ=255)
                                                                                                                              aaTCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCa                     >  1:62432/1‑148 (MQ=255)
                                                                                                                              aaTCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCa                     <  2:423930/148‑1 (MQ=255)
                                                                                                                               aTCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCaa                    >  1:2410601/1‑148 (MQ=255)
                                                                                                                                tCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAg                   >  1:3304442/1‑148 (MQ=255)
                                                                                                                                tCCTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAg                   >  1:659409/1‑148 (MQ=255)
                                                                                                                                 ccTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGt                  >  2:1807881/1‑148 (MQ=255)
                                                                                                                                  cTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTc                 <  2:2489715/148‑1 (MQ=255)
                                                                                                                                  cTGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTc                 <  2:727238/148‑1 (MQ=255)
                                                                                                                                   tGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCt                >  2:2946210/1‑148 (MQ=255)
                                                                                                                                   tGATCCCATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCt                >  1:1669329/1‑148 (MQ=255)
                                                                                                                                       cccATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTa            >  1:2892178/1‑148 (MQ=255)
                                                                                                                                       cccATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTa            <  2:2334256/148‑1 (MQ=255)
                                                                                                                                       cccATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTa            >  1:663121/1‑148 (MQ=255)
                                                                                                                                       cccATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTa            <  2:1935847/148‑1 (MQ=255)
                                                                                                                                         cATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGt          >  1:2832337/1‑148 (MQ=255)
                                                                                                                                         cATTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGt          >  1:1037757/1‑148 (MQ=255)
                                                                                                                                          aTTCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGtt         >  2:2899808/1‑148 (MQ=255)
                                                                                                                                           ttCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGttt        >  2:2274414/1‑148 (MQ=255)
                                                                                                                                            tCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTc       >  1:3248927/1‑148 (MQ=255)
                                                                                                                                            tCTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTc       <  1:2291103/148‑1 (MQ=255)
                                                                                                                                             cTGTGTTATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTcc      <  2:743255/148‑1 (MQ=255)
                                                                                                                                             cTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTcc      <  2:2412834/148‑1 (MQ=255)
                                                                                                                                                tgttATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTgg   <  2:1871218/148‑1 (MQ=255)
                                                                                                                                                 gtgATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTGGc  <  1:3038681/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCATTCTGTGTGATGGCGATTGCCAATGGCGCGATCTACCCTATTGTTGTCGCCCAGGCGCTGCGTCCCTTCCCACACGCAACTGGTCGCGCCGCAGCGTTGCAGAACACTCTTCAACTGGGTCTGTGCTTCCTCGCAAGTCTGGTAGTTTCCTGGC  >  CP009273/1734935‑1735227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑